Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion

Detalhes bibliográficos
Autor(a) principal: Fernandes, Maria Cecília
Data de Publicação: 2011
Outros Autores: Andrade, Leonardo Rodrigues De, Andrews, Norma Windsor, Mortara, Renato Arruda [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0074-02762011000800019
http://repositorio.unifesp.br/handle/11600/6720
Resumo: It has been recently shown that Trypanosoma cruzi trypomastigotes subvert a constitutive membrane repair mechanism to invade HeLa cells. Using a membrane extraction protocol and high-resolution microscopy, the HeLa cytoskeleton and T. cruzi parasites were imaged during the invasion process after 15 min and 45 min. Parasites were initially found under cells and were later observed in the cytoplasm. At later stages, parasite-driven protrusions with parallel filaments were observed, with trypomastigotes at their tips. We conclude that T. cruzi trypomastigotes induce deformations of the cortical actin cytoskeleton shortly after invasion, leading to the formation of pseudopod-like structures.
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spelling Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasionTrypanosoma cruzicell invasioncytoskeletonmembrane protrusionIt has been recently shown that Trypanosoma cruzi trypomastigotes subvert a constitutive membrane repair mechanism to invade HeLa cells. Using a membrane extraction protocol and high-resolution microscopy, the HeLa cytoskeleton and T. cruzi parasites were imaged during the invasion process after 15 min and 45 min. Parasites were initially found under cells and were later observed in the cytoplasm. At later stages, parasite-driven protrusions with parallel filaments were observed, with trypomastigotes at their tips. We conclude that T. cruzi trypomastigotes induce deformations of the cortical actin cytoskeleton shortly after invasion, leading to the formation of pseudopod-like structures.University of Maryland Department of Cell Biology and GeneticsNational Institutes of Health National Institute on Deafness and Other Communication Disorder Laboratory of Cell Structure and Dynamics, Section on Structural Cell BiologyUniversidade Federal do Rio de Janeiro Centro de Ciências da Saúde Instituto de Ciências BiomédicUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Microbiologia, Imunologia e ParasitologiaUNIFESP, EPM, Depto. de Microbiologia, Imunologia e ParasitologiaSciELOInstituto Oswaldo Cruz, Ministério da SaúdeUniversity of Maryland Department of Cell Biology and GeneticsNational Institutes of Health National Institute on Deafness and Other Communication Disorder Laboratory of Cell Structure and Dynamics, Section on Structural Cell BiologyUniversidade Federal do Rio de Janeiro Centro de Ciências da Saúde Instituto de Ciências BiomédicUniversidade Federal de São Paulo (UNIFESP)Fernandes, Maria CecíliaAndrade, Leonardo Rodrigues DeAndrews, Norma WindsorMortara, Renato Arruda [UNIFESP]2015-06-14T13:43:22Z2015-06-14T13:43:22Z2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1014-1016application/pdfhttp://dx.doi.org/10.1590/S0074-02762011000800019Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 106, n. 8, p. 1014-1016, 2011.10.1590/S0074-02762011000800019S0074-02762011000800019.pdf0074-0276S0074-02762011000800019http://repositorio.unifesp.br/handle/11600/6720WOS:000299068800019engMemórias do Instituto Oswaldo Cruzinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-28T23:16:35Zoai:repositorio.unifesp.br/:11600/6720Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-28T23:16:35Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
title Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
spellingShingle Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
Fernandes, Maria Cecília
Trypanosoma cruzi
cell invasion
cytoskeleton
membrane protrusion
title_short Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
title_full Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
title_fullStr Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
title_full_unstemmed Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
title_sort Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
author Fernandes, Maria Cecília
author_facet Fernandes, Maria Cecília
Andrade, Leonardo Rodrigues De
Andrews, Norma Windsor
Mortara, Renato Arruda [UNIFESP]
author_role author
author2 Andrade, Leonardo Rodrigues De
Andrews, Norma Windsor
Mortara, Renato Arruda [UNIFESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv University of Maryland Department of Cell Biology and Genetics
National Institutes of Health National Institute on Deafness and Other Communication Disorder Laboratory of Cell Structure and Dynamics, Section on Structural Cell Biology
Universidade Federal do Rio de Janeiro Centro de Ciências da Saúde Instituto de Ciências Biomédic
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Fernandes, Maria Cecília
Andrade, Leonardo Rodrigues De
Andrews, Norma Windsor
Mortara, Renato Arruda [UNIFESP]
dc.subject.por.fl_str_mv Trypanosoma cruzi
cell invasion
cytoskeleton
membrane protrusion
topic Trypanosoma cruzi
cell invasion
cytoskeleton
membrane protrusion
description It has been recently shown that Trypanosoma cruzi trypomastigotes subvert a constitutive membrane repair mechanism to invade HeLa cells. Using a membrane extraction protocol and high-resolution microscopy, the HeLa cytoskeleton and T. cruzi parasites were imaged during the invasion process after 15 min and 45 min. Parasites were initially found under cells and were later observed in the cytoplasm. At later stages, parasite-driven protrusions with parallel filaments were observed, with trypomastigotes at their tips. We conclude that T. cruzi trypomastigotes induce deformations of the cortical actin cytoskeleton shortly after invasion, leading to the formation of pseudopod-like structures.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
2015-06-14T13:43:22Z
2015-06-14T13:43:22Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0074-02762011000800019
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 106, n. 8, p. 1014-1016, 2011.
10.1590/S0074-02762011000800019
S0074-02762011000800019.pdf
0074-0276
S0074-02762011000800019
http://repositorio.unifesp.br/handle/11600/6720
WOS:000299068800019
url http://dx.doi.org/10.1590/S0074-02762011000800019
http://repositorio.unifesp.br/handle/11600/6720
identifier_str_mv Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 106, n. 8, p. 1014-1016, 2011.
10.1590/S0074-02762011000800019
S0074-02762011000800019.pdf
0074-0276
S0074-02762011000800019
WOS:000299068800019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1014-1016
application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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