Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion

Detalhes bibliográficos
Autor(a) principal: Fernandes,Maria Cecília
Data de Publicação: 2011
Outros Autores: Andrade,Leonardo Rodrigues de, Andrews,Norma Windsor, Mortara,Renato Arruda
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000800019
Resumo: It has been recently shown that Trypanosoma cruzi trypomastigotes subvert a constitutive membrane repair mechanism to invade HeLa cells. Using a membrane extraction protocol and high-resolution microscopy, the HeLa cytoskeleton and T. cruzi parasites were imaged during the invasion process after 15 min and 45 min. Parasites were initially found under cells and were later observed in the cytoplasm. At later stages, parasite-driven protrusions with parallel filaments were observed, with trypomastigotes at their tips. We conclude that T. cruzi trypomastigotes induce deformations of the cortical actin cytoskeleton shortly after invasion, leading to the formation of pseudopod-like structures.
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spelling Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasionTrypanosoma cruzicell invasioncytoskeletonmembrane protrusionIt has been recently shown that Trypanosoma cruzi trypomastigotes subvert a constitutive membrane repair mechanism to invade HeLa cells. Using a membrane extraction protocol and high-resolution microscopy, the HeLa cytoskeleton and T. cruzi parasites were imaged during the invasion process after 15 min and 45 min. Parasites were initially found under cells and were later observed in the cytoplasm. At later stages, parasite-driven protrusions with parallel filaments were observed, with trypomastigotes at their tips. We conclude that T. cruzi trypomastigotes induce deformations of the cortical actin cytoskeleton shortly after invasion, leading to the formation of pseudopod-like structures.Instituto Oswaldo Cruz, Ministério da Saúde2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000800019Memórias do Instituto Oswaldo Cruz v.106 n.8 2011reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762011000800019info:eu-repo/semantics/openAccessFernandes,Maria CecíliaAndrade,Leonardo Rodrigues deAndrews,Norma WindsorMortara,Renato Arrudaeng2020-04-25T17:51:05Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:59.468Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
title Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
spellingShingle Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
Fernandes,Maria Cecília
Trypanosoma cruzi
cell invasion
cytoskeleton
membrane protrusion
title_short Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
title_full Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
title_fullStr Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
title_full_unstemmed Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
title_sort Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
author Fernandes,Maria Cecília
author_facet Fernandes,Maria Cecília
Andrade,Leonardo Rodrigues de
Andrews,Norma Windsor
Mortara,Renato Arruda
author_role author
author2 Andrade,Leonardo Rodrigues de
Andrews,Norma Windsor
Mortara,Renato Arruda
author2_role author
author
author
dc.contributor.author.fl_str_mv Fernandes,Maria Cecília
Andrade,Leonardo Rodrigues de
Andrews,Norma Windsor
Mortara,Renato Arruda
dc.subject.por.fl_str_mv Trypanosoma cruzi
cell invasion
cytoskeleton
membrane protrusion
topic Trypanosoma cruzi
cell invasion
cytoskeleton
membrane protrusion
dc.description.none.fl_txt_mv It has been recently shown that Trypanosoma cruzi trypomastigotes subvert a constitutive membrane repair mechanism to invade HeLa cells. Using a membrane extraction protocol and high-resolution microscopy, the HeLa cytoskeleton and T. cruzi parasites were imaged during the invasion process after 15 min and 45 min. Parasites were initially found under cells and were later observed in the cytoplasm. At later stages, parasite-driven protrusions with parallel filaments were observed, with trypomastigotes at their tips. We conclude that T. cruzi trypomastigotes induce deformations of the cortical actin cytoskeleton shortly after invasion, leading to the formation of pseudopod-like structures.
description It has been recently shown that Trypanosoma cruzi trypomastigotes subvert a constitutive membrane repair mechanism to invade HeLa cells. Using a membrane extraction protocol and high-resolution microscopy, the HeLa cytoskeleton and T. cruzi parasites were imaged during the invasion process after 15 min and 45 min. Parasites were initially found under cells and were later observed in the cytoplasm. At later stages, parasite-driven protrusions with parallel filaments were observed, with trypomastigotes at their tips. We conclude that T. cruzi trypomastigotes induce deformations of the cortical actin cytoskeleton shortly after invasion, leading to the formation of pseudopod-like structures.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000800019
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000800019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762011000800019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.106 n.8 2011
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
_version_ 1669937710960214016

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