Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis
Autor(a) principal: | |
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Data de Publicação: | 1996 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://dx.doi.org/10.1080/02681219680000451 https://repositorio.unifesp.br/handle/11600/44493 |
Resumo: | The exoantigen of Paracoccidioides brasiliensis standardized by Camargo et al. [1] (AgR) was used to evaluate the in vivo and in vitro cell immune response of experimental animals and of patients with paracoccidioidomycosis (PBM). Fava Netto antigen (AgF) was tested in parallel as a control antigen. The study was conducted with mice and guinea pigs infected with P. brasiliensis or immunized with its fungal antigens, on patients with PBM and on their respective control groups. The cell immune response was analysed by skin tests, and by the macrophage and leucocyte migration inhibition tests (MMIT and LMIT) in the animals and in the patients, respectively. The skin test with AgR as paracoccidioidin was positive in infected or immunized mice and guinea pigs and negative in control animals. The skin tests with AgR (24 h) showed 96.7% positivity in patients with PBM and were negative in control individuals. Histopathological study of the in vivo tests in the different experimental models was consistent with a delayed hypersensitivity response (DHR). Immunohistochemical study of the skin tests of PBM patients demonstrated a predominance of T lymphocytes, confirming the nature of a DHR to the fungal antigens. The in vitro cell immune response showed variable results for the various experimental models, i.e. significant rates of MMIT in immunized mice, a tendency to positivity in infected guinea pigs, and the absence of migration inhibition in PBM patients. Taken together, the data indicate that the AgR is efficient as paracoccidioidin in the evaluation of DHR in PBM, with an optimum time of reading the test of 24 h. |
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Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosisParacoccidioides brasiliensisParacoccidioidinParacoccidioidomycosisThe exoantigen of Paracoccidioides brasiliensis standardized by Camargo et al. [1] (AgR) was used to evaluate the in vivo and in vitro cell immune response of experimental animals and of patients with paracoccidioidomycosis (PBM). Fava Netto antigen (AgF) was tested in parallel as a control antigen. The study was conducted with mice and guinea pigs infected with P. brasiliensis or immunized with its fungal antigens, on patients with PBM and on their respective control groups. The cell immune response was analysed by skin tests, and by the macrophage and leucocyte migration inhibition tests (MMIT and LMIT) in the animals and in the patients, respectively. The skin test with AgR as paracoccidioidin was positive in infected or immunized mice and guinea pigs and negative in control animals. The skin tests with AgR (24 h) showed 96.7% positivity in patients with PBM and were negative in control individuals. Histopathological study of the in vivo tests in the different experimental models was consistent with a delayed hypersensitivity response (DHR). Immunohistochemical study of the skin tests of PBM patients demonstrated a predominance of T lymphocytes, confirming the nature of a DHR to the fungal antigens. The in vitro cell immune response showed variable results for the various experimental models, i.e. significant rates of MMIT in immunized mice, a tendency to positivity in infected guinea pigs, and the absence of migration inhibition in PBM patients. Taken together, the data indicate that the AgR is efficient as paracoccidioidin in the evaluation of DHR in PBM, with an optimum time of reading the test of 24 h.UNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FAC MED BOTUCATU,DEPT PATOL,BOTUCATU,SP,BRAZILUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,INST BIOCIENCIAS,DIV IMUNOL,BOTUCATU,SP,BRAZILESCOLA PAULISTA MED,DIV BIOL CELULAR,SAO PAULO,BRAZILUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FAC MED BOTCATU,DEPT DERMATOL,BOTUCATU,SP,BRAZILUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FAC MED BOTCATU,DEPT MOLESTIAS INFECC,BOTUCATU,SP,BRAZILESCOLA PAULISTA MED,DIV BIOL CELULAR,SAO PAULO,BRAZILWeb of ScienceBlackwell Science LtdUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Marques, Mariangela Esther AlencarSoares, Angela Maria Victoriano de CamposFranco, MarcelloCamargo, Zoilo Pires de [UNIFESP]Marques, Silvio AlencarMendes, Rinaldo PoncioSouza, Lenice do Rosário deCoelho, Kunie Iabuki Rabello2018-06-15T18:07:24Z2018-06-15T18:07:24Z1996-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion265-272https://dx.doi.org/10.1080/02681219680000451Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 4, p. 265-272, 1996.10.1080/026812196800004510268-1218https://repositorio.unifesp.br/handle/11600/44493WOS:A1996VC30200006engJournal Of Medical And Veterinary Mycologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-09T09:42:18Zoai:repositorio.unifesp.br/:11600/44493Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-09T09:42:18Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis |
title |
Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis |
spellingShingle |
Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis Marques, Mariangela Esther Alencar Paracoccidioides brasiliensis Paracoccidioidin Paracoccidioidomycosis |
title_short |
Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis |
title_full |
Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis |
title_fullStr |
Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis |
title_full_unstemmed |
Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis |
title_sort |
Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis |
author |
Marques, Mariangela Esther Alencar |
author_facet |
Marques, Mariangela Esther Alencar Soares, Angela Maria Victoriano de Campos Franco, Marcello Camargo, Zoilo Pires de [UNIFESP] Marques, Silvio Alencar Mendes, Rinaldo Poncio Souza, Lenice do Rosário de Coelho, Kunie Iabuki Rabello |
author_role |
author |
author2 |
Soares, Angela Maria Victoriano de Campos Franco, Marcello Camargo, Zoilo Pires de [UNIFESP] Marques, Silvio Alencar Mendes, Rinaldo Poncio Souza, Lenice do Rosário de Coelho, Kunie Iabuki Rabello |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Marques, Mariangela Esther Alencar Soares, Angela Maria Victoriano de Campos Franco, Marcello Camargo, Zoilo Pires de [UNIFESP] Marques, Silvio Alencar Mendes, Rinaldo Poncio Souza, Lenice do Rosário de Coelho, Kunie Iabuki Rabello |
dc.subject.por.fl_str_mv |
Paracoccidioides brasiliensis Paracoccidioidin Paracoccidioidomycosis |
topic |
Paracoccidioides brasiliensis Paracoccidioidin Paracoccidioidomycosis |
description |
The exoantigen of Paracoccidioides brasiliensis standardized by Camargo et al. [1] (AgR) was used to evaluate the in vivo and in vitro cell immune response of experimental animals and of patients with paracoccidioidomycosis (PBM). Fava Netto antigen (AgF) was tested in parallel as a control antigen. The study was conducted with mice and guinea pigs infected with P. brasiliensis or immunized with its fungal antigens, on patients with PBM and on their respective control groups. The cell immune response was analysed by skin tests, and by the macrophage and leucocyte migration inhibition tests (MMIT and LMIT) in the animals and in the patients, respectively. The skin test with AgR as paracoccidioidin was positive in infected or immunized mice and guinea pigs and negative in control animals. The skin tests with AgR (24 h) showed 96.7% positivity in patients with PBM and were negative in control individuals. Histopathological study of the in vivo tests in the different experimental models was consistent with a delayed hypersensitivity response (DHR). Immunohistochemical study of the skin tests of PBM patients demonstrated a predominance of T lymphocytes, confirming the nature of a DHR to the fungal antigens. The in vitro cell immune response showed variable results for the various experimental models, i.e. significant rates of MMIT in immunized mice, a tendency to positivity in infected guinea pigs, and the absence of migration inhibition in PBM patients. Taken together, the data indicate that the AgR is efficient as paracoccidioidin in the evaluation of DHR in PBM, with an optimum time of reading the test of 24 h. |
publishDate |
1996 |
dc.date.none.fl_str_mv |
1996-07-01 2018-06-15T18:07:24Z 2018-06-15T18:07:24Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://dx.doi.org/10.1080/02681219680000451 Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 4, p. 265-272, 1996. 10.1080/02681219680000451 0268-1218 https://repositorio.unifesp.br/handle/11600/44493 WOS:A1996VC30200006 |
url |
https://dx.doi.org/10.1080/02681219680000451 https://repositorio.unifesp.br/handle/11600/44493 |
identifier_str_mv |
Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 4, p. 265-272, 1996. 10.1080/02681219680000451 0268-1218 WOS:A1996VC30200006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Medical And Veterinary Mycology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
265-272 |
dc.publisher.none.fl_str_mv |
Blackwell Science Ltd |
publisher.none.fl_str_mv |
Blackwell Science Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268443707310080 |