Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis

Detalhes bibliográficos
Autor(a) principal: Marques, Mariangela Esther Alencar
Data de Publicação: 1996
Outros Autores: Soares, Angela Maria Victoriano de Campos, Franco, Marcello, Camargo, Zoilo Pires de [UNIFESP], Marques, Silvio Alencar, Mendes, Rinaldo Poncio, Souza, Lenice do Rosário de, Coelho, Kunie Iabuki Rabello
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://dx.doi.org/10.1080/02681219680000451
https://repositorio.unifesp.br/handle/11600/44493
Resumo: The exoantigen of Paracoccidioides brasiliensis standardized by Camargo et al. [1] (AgR) was used to evaluate the in vivo and in vitro cell immune response of experimental animals and of patients with paracoccidioidomycosis (PBM). Fava Netto antigen (AgF) was tested in parallel as a control antigen. The study was conducted with mice and guinea pigs infected with P. brasiliensis or immunized with its fungal antigens, on patients with PBM and on their respective control groups. The cell immune response was analysed by skin tests, and by the macrophage and leucocyte migration inhibition tests (MMIT and LMIT) in the animals and in the patients, respectively. The skin test with AgR as paracoccidioidin was positive in infected or immunized mice and guinea pigs and negative in control animals. The skin tests with AgR (24 h) showed 96.7% positivity in patients with PBM and were negative in control individuals. Histopathological study of the in vivo tests in the different experimental models was consistent with a delayed hypersensitivity response (DHR). Immunohistochemical study of the skin tests of PBM patients demonstrated a predominance of T lymphocytes, confirming the nature of a DHR to the fungal antigens. The in vitro cell immune response showed variable results for the various experimental models, i.e. significant rates of MMIT in immunized mice, a tendency to positivity in infected guinea pigs, and the absence of migration inhibition in PBM patients. Taken together, the data indicate that the AgR is efficient as paracoccidioidin in the evaluation of DHR in PBM, with an optimum time of reading the test of 24 h.
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spelling Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosisParacoccidioides brasiliensisParacoccidioidinParacoccidioidomycosisThe exoantigen of Paracoccidioides brasiliensis standardized by Camargo et al. [1] (AgR) was used to evaluate the in vivo and in vitro cell immune response of experimental animals and of patients with paracoccidioidomycosis (PBM). Fava Netto antigen (AgF) was tested in parallel as a control antigen. The study was conducted with mice and guinea pigs infected with P. brasiliensis or immunized with its fungal antigens, on patients with PBM and on their respective control groups. The cell immune response was analysed by skin tests, and by the macrophage and leucocyte migration inhibition tests (MMIT and LMIT) in the animals and in the patients, respectively. The skin test with AgR as paracoccidioidin was positive in infected or immunized mice and guinea pigs and negative in control animals. The skin tests with AgR (24 h) showed 96.7% positivity in patients with PBM and were negative in control individuals. Histopathological study of the in vivo tests in the different experimental models was consistent with a delayed hypersensitivity response (DHR). Immunohistochemical study of the skin tests of PBM patients demonstrated a predominance of T lymphocytes, confirming the nature of a DHR to the fungal antigens. The in vitro cell immune response showed variable results for the various experimental models, i.e. significant rates of MMIT in immunized mice, a tendency to positivity in infected guinea pigs, and the absence of migration inhibition in PBM patients. Taken together, the data indicate that the AgR is efficient as paracoccidioidin in the evaluation of DHR in PBM, with an optimum time of reading the test of 24 h.UNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FAC MED BOTUCATU,DEPT PATOL,BOTUCATU,SP,BRAZILUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,INST BIOCIENCIAS,DIV IMUNOL,BOTUCATU,SP,BRAZILESCOLA PAULISTA MED,DIV BIOL CELULAR,SAO PAULO,BRAZILUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FAC MED BOTCATU,DEPT DERMATOL,BOTUCATU,SP,BRAZILUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FAC MED BOTCATU,DEPT MOLESTIAS INFECC,BOTUCATU,SP,BRAZILESCOLA PAULISTA MED,DIV BIOL CELULAR,SAO PAULO,BRAZILWeb of ScienceBlackwell Science LtdUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Marques, Mariangela Esther AlencarSoares, Angela Maria Victoriano de CamposFranco, MarcelloCamargo, Zoilo Pires de [UNIFESP]Marques, Silvio AlencarMendes, Rinaldo PoncioSouza, Lenice do Rosário deCoelho, Kunie Iabuki Rabello2018-06-15T18:07:24Z2018-06-15T18:07:24Z1996-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion265-272https://dx.doi.org/10.1080/02681219680000451Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 4, p. 265-272, 1996.10.1080/026812196800004510268-1218https://repositorio.unifesp.br/handle/11600/44493WOS:A1996VC30200006engJournal Of Medical And Veterinary Mycologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-09T09:42:18Zoai:repositorio.unifesp.br/:11600/44493Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-09T09:42:18Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis
title Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis
spellingShingle Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis
Marques, Mariangela Esther Alencar
Paracoccidioides brasiliensis
Paracoccidioidin
Paracoccidioidomycosis
title_short Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis
title_full Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis
title_fullStr Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis
title_full_unstemmed Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis
title_sort Evaluation of Paracoccidioides brasiliensis exoantigen in the detection of delayed dermal hypersensitivity in experimental and human paracoccidioidomycosis
author Marques, Mariangela Esther Alencar
author_facet Marques, Mariangela Esther Alencar
Soares, Angela Maria Victoriano de Campos
Franco, Marcello
Camargo, Zoilo Pires de [UNIFESP]
Marques, Silvio Alencar
Mendes, Rinaldo Poncio
Souza, Lenice do Rosário de
Coelho, Kunie Iabuki Rabello
author_role author
author2 Soares, Angela Maria Victoriano de Campos
Franco, Marcello
Camargo, Zoilo Pires de [UNIFESP]
Marques, Silvio Alencar
Mendes, Rinaldo Poncio
Souza, Lenice do Rosário de
Coelho, Kunie Iabuki Rabello
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Marques, Mariangela Esther Alencar
Soares, Angela Maria Victoriano de Campos
Franco, Marcello
Camargo, Zoilo Pires de [UNIFESP]
Marques, Silvio Alencar
Mendes, Rinaldo Poncio
Souza, Lenice do Rosário de
Coelho, Kunie Iabuki Rabello
dc.subject.por.fl_str_mv Paracoccidioides brasiliensis
Paracoccidioidin
Paracoccidioidomycosis
topic Paracoccidioides brasiliensis
Paracoccidioidin
Paracoccidioidomycosis
description The exoantigen of Paracoccidioides brasiliensis standardized by Camargo et al. [1] (AgR) was used to evaluate the in vivo and in vitro cell immune response of experimental animals and of patients with paracoccidioidomycosis (PBM). Fava Netto antigen (AgF) was tested in parallel as a control antigen. The study was conducted with mice and guinea pigs infected with P. brasiliensis or immunized with its fungal antigens, on patients with PBM and on their respective control groups. The cell immune response was analysed by skin tests, and by the macrophage and leucocyte migration inhibition tests (MMIT and LMIT) in the animals and in the patients, respectively. The skin test with AgR as paracoccidioidin was positive in infected or immunized mice and guinea pigs and negative in control animals. The skin tests with AgR (24 h) showed 96.7% positivity in patients with PBM and were negative in control individuals. Histopathological study of the in vivo tests in the different experimental models was consistent with a delayed hypersensitivity response (DHR). Immunohistochemical study of the skin tests of PBM patients demonstrated a predominance of T lymphocytes, confirming the nature of a DHR to the fungal antigens. The in vitro cell immune response showed variable results for the various experimental models, i.e. significant rates of MMIT in immunized mice, a tendency to positivity in infected guinea pigs, and the absence of migration inhibition in PBM patients. Taken together, the data indicate that the AgR is efficient as paracoccidioidin in the evaluation of DHR in PBM, with an optimum time of reading the test of 24 h.
publishDate 1996
dc.date.none.fl_str_mv 1996-07-01
2018-06-15T18:07:24Z
2018-06-15T18:07:24Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://dx.doi.org/10.1080/02681219680000451
Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 4, p. 265-272, 1996.
10.1080/02681219680000451
0268-1218
https://repositorio.unifesp.br/handle/11600/44493
WOS:A1996VC30200006
url https://dx.doi.org/10.1080/02681219680000451
https://repositorio.unifesp.br/handle/11600/44493
identifier_str_mv Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 4, p. 265-272, 1996.
10.1080/02681219680000451
0268-1218
WOS:A1996VC30200006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Medical And Veterinary Mycology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 265-272
dc.publisher.none.fl_str_mv Blackwell Science Ltd
publisher.none.fl_str_mv Blackwell Science Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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