Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/34554 http://dx.doi.org/10.1371/journal.pntd.0001519 |
Resumo: | Paracoccidioidomycosis (PCM), caused by Paracoccidioides brasiliensis, is the most prevalent invasive fungal disease in South America. Systemic mycoses are the 10th most common cause of death among infectious diseases in Brazil and PCM is responsible for more than 50% of deaths due to fungal infections. PCM is typically treated with sulfonamides, amphotericin B or azoles, although complete eradication of the fungus may not occur and relapsing disease is frequently reported. A 15-mer peptide from the major diagnostic antigen gp43, named P10, can induce a strong T-CD4+ helper-1 immune response in mice. the TEPITOPE algorithm and experimental data have confirmed that most HLA-DR molecules can present P10, which suggests that P10 is a candidate antigen for a PCM vaccine. in the current work, the therapeutic efficacy of plasmid immunization with P10 and/or IL-12 inserts was tested in murine models of PCM. When given prior to or after infection with P. brasiliensis virulent Pb 18 isolate, plasmid-vaccination with P10 and/or IL-12 inserts successfully reduced the fungal burden in lungs of infected mice. in fact, intramuscular administration of a combination of plasmids expressing P10 and IL-12 given weekly for one month, followed by single injections every month for 3 months restored normal lung architecture and eradicated the fungus in mice that were infected one month prior to treatment. the data indicate that immunization with these plasmids is a powerful procedure for prevention and treatment of experimental PCM, with the perspective of being also effective in human patients. |
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Rittner, Glauce M. G.Munoz, Julian E.Marques, Alexandre F.Nosanchuk, Joshua D.Taborda, Carlos Pelleschi [UNIFESP]Travassos, Luiz R. [UNIFESP]Universidade de São Paulo (USP)Albert Einstein Coll MedUniversidade Federal de São Paulo (UNIFESP)2016-01-24T14:17:50Z2016-01-24T14:17:50Z2012-02-01Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 6, n. 2, 9 p., 2012.1935-2735http://repositorio.unifesp.br/handle/11600/34554http://dx.doi.org/10.1371/journal.pntd.0001519WOS000300949200031.pdf10.1371/journal.pntd.0001519WOS:000300949200031Paracoccidioidomycosis (PCM), caused by Paracoccidioides brasiliensis, is the most prevalent invasive fungal disease in South America. Systemic mycoses are the 10th most common cause of death among infectious diseases in Brazil and PCM is responsible for more than 50% of deaths due to fungal infections. PCM is typically treated with sulfonamides, amphotericin B or azoles, although complete eradication of the fungus may not occur and relapsing disease is frequently reported. A 15-mer peptide from the major diagnostic antigen gp43, named P10, can induce a strong T-CD4+ helper-1 immune response in mice. the TEPITOPE algorithm and experimental data have confirmed that most HLA-DR molecules can present P10, which suggests that P10 is a candidate antigen for a PCM vaccine. in the current work, the therapeutic efficacy of plasmid immunization with P10 and/or IL-12 inserts was tested in murine models of PCM. When given prior to or after infection with P. brasiliensis virulent Pb 18 isolate, plasmid-vaccination with P10 and/or IL-12 inserts successfully reduced the fungal burden in lungs of infected mice. in fact, intramuscular administration of a combination of plasmids expressing P10 and IL-12 given weekly for one month, followed by single injections every month for 3 months restored normal lung architecture and eradicated the fungus in mice that were infected one month prior to treatment. the data indicate that immunization with these plasmids is a powerful procedure for prevention and treatment of experimental PCM, with the perspective of being also effective in human patients.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ São Paulo, Inst Biomed Sci, Dept Microbiol, São Paulo, BrazilAlbert Einstein Coll Med, Dept Med, Bronx, NY 10467 USAAlbert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10467 USAUniv São Paulo, Med Mycol Lab IMTSP & HCFMUSP LIM53, São Paulo, BrazilFed Univ São Paulo UNIFESP, Div Cell Biol, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilFed Univ São Paulo UNIFESP, Div Cell Biol, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilFAPESP: 09/15823-7FAPESP: 07/07588-2FAPESP: 06/50634-2FAPESP: 05/02776-0CNPq: 470513/2009-8Web of Science9engPublic Library SciencePlos Neglected Tropical DiseasesTherapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000300949200031.pdfapplication/pdf409154${dspace.ui.url}/bitstream/11600/34554/1/WOS000300949200031.pdf2a592101c1dce93355ee2d5e84128a09MD51open accessTEXTWOS000300949200031.pdf.txtWOS000300949200031.pdf.txtExtracted texttext/plain41379${dspace.ui.url}/bitstream/11600/34554/2/WOS000300949200031.pdf.txtab1a642ddfc7ce9eeb83b086be6657aaMD52open access11600/345542022-02-08 11:53:18.954open accessoai:repositorio.unifesp.br:11600/34554Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-02-08T14:53:18Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis |
title |
Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis |
spellingShingle |
Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis Rittner, Glauce M. G. |
title_short |
Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis |
title_full |
Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis |
title_fullStr |
Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis |
title_full_unstemmed |
Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis |
title_sort |
Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis |
author |
Rittner, Glauce M. G. |
author_facet |
Rittner, Glauce M. G. Munoz, Julian E. Marques, Alexandre F. Nosanchuk, Joshua D. Taborda, Carlos Pelleschi [UNIFESP] Travassos, Luiz R. [UNIFESP] |
author_role |
author |
author2 |
Munoz, Julian E. Marques, Alexandre F. Nosanchuk, Joshua D. Taborda, Carlos Pelleschi [UNIFESP] Travassos, Luiz R. [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade de São Paulo (USP) Albert Einstein Coll Med Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Rittner, Glauce M. G. Munoz, Julian E. Marques, Alexandre F. Nosanchuk, Joshua D. Taborda, Carlos Pelleschi [UNIFESP] Travassos, Luiz R. [UNIFESP] |
description |
Paracoccidioidomycosis (PCM), caused by Paracoccidioides brasiliensis, is the most prevalent invasive fungal disease in South America. Systemic mycoses are the 10th most common cause of death among infectious diseases in Brazil and PCM is responsible for more than 50% of deaths due to fungal infections. PCM is typically treated with sulfonamides, amphotericin B or azoles, although complete eradication of the fungus may not occur and relapsing disease is frequently reported. A 15-mer peptide from the major diagnostic antigen gp43, named P10, can induce a strong T-CD4+ helper-1 immune response in mice. the TEPITOPE algorithm and experimental data have confirmed that most HLA-DR molecules can present P10, which suggests that P10 is a candidate antigen for a PCM vaccine. in the current work, the therapeutic efficacy of plasmid immunization with P10 and/or IL-12 inserts was tested in murine models of PCM. When given prior to or after infection with P. brasiliensis virulent Pb 18 isolate, plasmid-vaccination with P10 and/or IL-12 inserts successfully reduced the fungal burden in lungs of infected mice. in fact, intramuscular administration of a combination of plasmids expressing P10 and IL-12 given weekly for one month, followed by single injections every month for 3 months restored normal lung architecture and eradicated the fungus in mice that were infected one month prior to treatment. the data indicate that immunization with these plasmids is a powerful procedure for prevention and treatment of experimental PCM, with the perspective of being also effective in human patients. |
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2012 |
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2016-01-24T14:17:50Z |
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Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 6, n. 2, 9 p., 2012. |
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http://repositorio.unifesp.br/handle/11600/34554 http://dx.doi.org/10.1371/journal.pntd.0001519 |
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1935-2735 |
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WOS000300949200031.pdf |
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Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 6, n. 2, 9 p., 2012. 1935-2735 WOS000300949200031.pdf 10.1371/journal.pntd.0001519 WOS:000300949200031 |
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