Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pntd.0001459 http://repositorio.unifesp.br/handle/11600/34393 |
Resumo: | Limited information is available regarding the modulation of genes involved in the innate host response to Paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis. Therefore, we sought to characterize, for the first time, the transcriptional profile of murine bone marrow-derived dendritic cells (DCs) at an early stage following their initial interaction with P. brasiliensis. DCs connect innate and adaptive immunity by recognizing invading pathogens and determining the type of effector T-cell that mediates an immune response. Gene expression profiles were analyzed using microarray and validated using real-time RT-PCR and protein secretion studies. A total of 299 genes were differentially expressed, many of which are involved in immunity, signal transduction, transcription and apoptosis. Genes encoding the cytokines IL-12 and TNF-alpha, along with the chemokines CCL22, CCL27 and CXCL10, were up-regulated, suggesting that P. brasiliensis induces a potent proinflammatory response in DCs. in contrast, pattern recognition receptor (PRR)-encoding genes, particularly those related to Toll-like receptors, were down-regulated or unchanged. This result prompted us to evaluate the expression profiles of dectin-1 and mannose receptor, two other important fungal PRRs that were not included in the microarray target cDNA sequences. Unlike the mannose receptor, the dectin-1 receptor gene was significantly induced, suggesting that this beta-glucan receptor participates in the recognition of P. brasiliensis. We also used a receptor inhibition assay to evaluate the roles of these receptors in coordinating the expression of several immune-related genes in DCs upon fungal exposure. Altogether, our results provide an initial characterization of early host responses to P. brasiliensis and a basis for better understanding the infectious process of this important neglected pathogen. |
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Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis InfectionLimited information is available regarding the modulation of genes involved in the innate host response to Paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis. Therefore, we sought to characterize, for the first time, the transcriptional profile of murine bone marrow-derived dendritic cells (DCs) at an early stage following their initial interaction with P. brasiliensis. DCs connect innate and adaptive immunity by recognizing invading pathogens and determining the type of effector T-cell that mediates an immune response. Gene expression profiles were analyzed using microarray and validated using real-time RT-PCR and protein secretion studies. A total of 299 genes were differentially expressed, many of which are involved in immunity, signal transduction, transcription and apoptosis. Genes encoding the cytokines IL-12 and TNF-alpha, along with the chemokines CCL22, CCL27 and CXCL10, were up-regulated, suggesting that P. brasiliensis induces a potent proinflammatory response in DCs. in contrast, pattern recognition receptor (PRR)-encoding genes, particularly those related to Toll-like receptors, were down-regulated or unchanged. This result prompted us to evaluate the expression profiles of dectin-1 and mannose receptor, two other important fungal PRRs that were not included in the microarray target cDNA sequences. Unlike the mannose receptor, the dectin-1 receptor gene was significantly induced, suggesting that this beta-glucan receptor participates in the recognition of P. brasiliensis. We also used a receptor inhibition assay to evaluate the roles of these receptors in coordinating the expression of several immune-related genes in DCs upon fungal exposure. Altogether, our results provide an initial characterization of early host responses to P. brasiliensis and a basis for better understanding the infectious process of this important neglected pathogen.Univ Brasilia, Fac Ceilandia, Brasilia, DF, BrazilUniv Brasilia, Dept Biol Celular, Brasilia, DF, BrazilUniversidade Federal de São Paulo, Dept Ciencias Biol, São Paulo, BrazilUniv São Paulo, Dept Genet, Fac Med Ribeirao Preto, São Paulo, BrazilUniv São Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Ciencias Biol, São Paulo, BrazilWeb of ScienceConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundacao de Amparo a Pesquisa do Distrito FederalCNPq: CNPQ-proc. no. 476554/2007-1Public Library ScienceUniversidade de Brasília (UnB)Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Tavares, Aldo H.Derengowski, Lorena S.Ferreira, Karen S. [UNIFESP]Silva, Simoneide S.Macedo, ClaudiaBocca, Anamelia L.Passos, Geraldo A.Almeida, Sandro R.Silva-Pereira, Ildinete2016-01-24T14:17:38Z2016-01-24T14:17:38Z2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion9application/pdfhttp://dx.doi.org/10.1371/journal.pntd.0001459Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 6, n. 1, 9 p., 2012.10.1371/journal.pntd.0001459WOS000300416100012.pdf1935-2727http://repositorio.unifesp.br/handle/11600/34393WOS:000300416100012engPlos Neglected Tropical Diseasesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T23:09:40Zoai:repositorio.unifesp.br/:11600/34393Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T23:09:40Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection |
title |
Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection |
spellingShingle |
Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection Tavares, Aldo H. |
title_short |
Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection |
title_full |
Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection |
title_fullStr |
Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection |
title_full_unstemmed |
Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection |
title_sort |
Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection |
author |
Tavares, Aldo H. |
author_facet |
Tavares, Aldo H. Derengowski, Lorena S. Ferreira, Karen S. [UNIFESP] Silva, Simoneide S. Macedo, Claudia Bocca, Anamelia L. Passos, Geraldo A. Almeida, Sandro R. Silva-Pereira, Ildinete |
author_role |
author |
author2 |
Derengowski, Lorena S. Ferreira, Karen S. [UNIFESP] Silva, Simoneide S. Macedo, Claudia Bocca, Anamelia L. Passos, Geraldo A. Almeida, Sandro R. Silva-Pereira, Ildinete |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de Brasília (UnB) Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Tavares, Aldo H. Derengowski, Lorena S. Ferreira, Karen S. [UNIFESP] Silva, Simoneide S. Macedo, Claudia Bocca, Anamelia L. Passos, Geraldo A. Almeida, Sandro R. Silva-Pereira, Ildinete |
description |
Limited information is available regarding the modulation of genes involved in the innate host response to Paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis. Therefore, we sought to characterize, for the first time, the transcriptional profile of murine bone marrow-derived dendritic cells (DCs) at an early stage following their initial interaction with P. brasiliensis. DCs connect innate and adaptive immunity by recognizing invading pathogens and determining the type of effector T-cell that mediates an immune response. Gene expression profiles were analyzed using microarray and validated using real-time RT-PCR and protein secretion studies. A total of 299 genes were differentially expressed, many of which are involved in immunity, signal transduction, transcription and apoptosis. Genes encoding the cytokines IL-12 and TNF-alpha, along with the chemokines CCL22, CCL27 and CXCL10, were up-regulated, suggesting that P. brasiliensis induces a potent proinflammatory response in DCs. in contrast, pattern recognition receptor (PRR)-encoding genes, particularly those related to Toll-like receptors, were down-regulated or unchanged. This result prompted us to evaluate the expression profiles of dectin-1 and mannose receptor, two other important fungal PRRs that were not included in the microarray target cDNA sequences. Unlike the mannose receptor, the dectin-1 receptor gene was significantly induced, suggesting that this beta-glucan receptor participates in the recognition of P. brasiliensis. We also used a receptor inhibition assay to evaluate the roles of these receptors in coordinating the expression of several immune-related genes in DCs upon fungal exposure. Altogether, our results provide an initial characterization of early host responses to P. brasiliensis and a basis for better understanding the infectious process of this important neglected pathogen. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 2016-01-24T14:17:38Z 2016-01-24T14:17:38Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pntd.0001459 Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 6, n. 1, 9 p., 2012. 10.1371/journal.pntd.0001459 WOS000300416100012.pdf 1935-2727 http://repositorio.unifesp.br/handle/11600/34393 WOS:000300416100012 |
url |
http://dx.doi.org/10.1371/journal.pntd.0001459 http://repositorio.unifesp.br/handle/11600/34393 |
identifier_str_mv |
Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 6, n. 1, 9 p., 2012. 10.1371/journal.pntd.0001459 WOS000300416100012.pdf 1935-2727 WOS:000300416100012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos Neglected Tropical Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268397014220800 |