Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection

Detalhes bibliográficos
Autor(a) principal: Tavares, Aldo H.
Data de Publicação: 2012
Outros Autores: Derengowski, Lorena S., Ferreira, Karen S. [UNIFESP], Silva, Simoneide S., Macedo, Claudia, Bocca, Anamelia L., Passos, Geraldo A., Almeida, Sandro R., Silva-Pereira, Ildinete
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1371/journal.pntd.0001459
http://repositorio.unifesp.br/handle/11600/34393
Resumo: Limited information is available regarding the modulation of genes involved in the innate host response to Paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis. Therefore, we sought to characterize, for the first time, the transcriptional profile of murine bone marrow-derived dendritic cells (DCs) at an early stage following their initial interaction with P. brasiliensis. DCs connect innate and adaptive immunity by recognizing invading pathogens and determining the type of effector T-cell that mediates an immune response. Gene expression profiles were analyzed using microarray and validated using real-time RT-PCR and protein secretion studies. A total of 299 genes were differentially expressed, many of which are involved in immunity, signal transduction, transcription and apoptosis. Genes encoding the cytokines IL-12 and TNF-alpha, along with the chemokines CCL22, CCL27 and CXCL10, were up-regulated, suggesting that P. brasiliensis induces a potent proinflammatory response in DCs. in contrast, pattern recognition receptor (PRR)-encoding genes, particularly those related to Toll-like receptors, were down-regulated or unchanged. This result prompted us to evaluate the expression profiles of dectin-1 and mannose receptor, two other important fungal PRRs that were not included in the microarray target cDNA sequences. Unlike the mannose receptor, the dectin-1 receptor gene was significantly induced, suggesting that this beta-glucan receptor participates in the recognition of P. brasiliensis. We also used a receptor inhibition assay to evaluate the roles of these receptors in coordinating the expression of several immune-related genes in DCs upon fungal exposure. Altogether, our results provide an initial characterization of early host responses to P. brasiliensis and a basis for better understanding the infectious process of this important neglected pathogen.
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spelling Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis InfectionLimited information is available regarding the modulation of genes involved in the innate host response to Paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis. Therefore, we sought to characterize, for the first time, the transcriptional profile of murine bone marrow-derived dendritic cells (DCs) at an early stage following their initial interaction with P. brasiliensis. DCs connect innate and adaptive immunity by recognizing invading pathogens and determining the type of effector T-cell that mediates an immune response. Gene expression profiles were analyzed using microarray and validated using real-time RT-PCR and protein secretion studies. A total of 299 genes were differentially expressed, many of which are involved in immunity, signal transduction, transcription and apoptosis. Genes encoding the cytokines IL-12 and TNF-alpha, along with the chemokines CCL22, CCL27 and CXCL10, were up-regulated, suggesting that P. brasiliensis induces a potent proinflammatory response in DCs. in contrast, pattern recognition receptor (PRR)-encoding genes, particularly those related to Toll-like receptors, were down-regulated or unchanged. This result prompted us to evaluate the expression profiles of dectin-1 and mannose receptor, two other important fungal PRRs that were not included in the microarray target cDNA sequences. Unlike the mannose receptor, the dectin-1 receptor gene was significantly induced, suggesting that this beta-glucan receptor participates in the recognition of P. brasiliensis. We also used a receptor inhibition assay to evaluate the roles of these receptors in coordinating the expression of several immune-related genes in DCs upon fungal exposure. Altogether, our results provide an initial characterization of early host responses to P. brasiliensis and a basis for better understanding the infectious process of this important neglected pathogen.Univ Brasilia, Fac Ceilandia, Brasilia, DF, BrazilUniv Brasilia, Dept Biol Celular, Brasilia, DF, BrazilUniversidade Federal de São Paulo, Dept Ciencias Biol, São Paulo, BrazilUniv São Paulo, Dept Genet, Fac Med Ribeirao Preto, São Paulo, BrazilUniv São Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Ciencias Biol, São Paulo, BrazilWeb of ScienceConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundacao de Amparo a Pesquisa do Distrito FederalCNPq: CNPQ-proc. no. 476554/2007-1Public Library ScienceUniversidade de Brasília (UnB)Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Tavares, Aldo H.Derengowski, Lorena S.Ferreira, Karen S. [UNIFESP]Silva, Simoneide S.Macedo, ClaudiaBocca, Anamelia L.Passos, Geraldo A.Almeida, Sandro R.Silva-Pereira, Ildinete2016-01-24T14:17:38Z2016-01-24T14:17:38Z2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion9application/pdfhttp://dx.doi.org/10.1371/journal.pntd.0001459Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 6, n. 1, 9 p., 2012.10.1371/journal.pntd.0001459WOS000300416100012.pdf1935-2727http://repositorio.unifesp.br/handle/11600/34393WOS:000300416100012engPlos Neglected Tropical Diseasesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T23:09:40Zoai:repositorio.unifesp.br/:11600/34393Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T23:09:40Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection
title Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection
spellingShingle Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection
Tavares, Aldo H.
title_short Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection
title_full Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection
title_fullStr Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection
title_full_unstemmed Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection
title_sort Murine Dendritic Cells Transcriptional Modulation upon Paracoccidioides brasiliensis Infection
author Tavares, Aldo H.
author_facet Tavares, Aldo H.
Derengowski, Lorena S.
Ferreira, Karen S. [UNIFESP]
Silva, Simoneide S.
Macedo, Claudia
Bocca, Anamelia L.
Passos, Geraldo A.
Almeida, Sandro R.
Silva-Pereira, Ildinete
author_role author
author2 Derengowski, Lorena S.
Ferreira, Karen S. [UNIFESP]
Silva, Simoneide S.
Macedo, Claudia
Bocca, Anamelia L.
Passos, Geraldo A.
Almeida, Sandro R.
Silva-Pereira, Ildinete
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Brasília (UnB)
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Tavares, Aldo H.
Derengowski, Lorena S.
Ferreira, Karen S. [UNIFESP]
Silva, Simoneide S.
Macedo, Claudia
Bocca, Anamelia L.
Passos, Geraldo A.
Almeida, Sandro R.
Silva-Pereira, Ildinete
description Limited information is available regarding the modulation of genes involved in the innate host response to Paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis. Therefore, we sought to characterize, for the first time, the transcriptional profile of murine bone marrow-derived dendritic cells (DCs) at an early stage following their initial interaction with P. brasiliensis. DCs connect innate and adaptive immunity by recognizing invading pathogens and determining the type of effector T-cell that mediates an immune response. Gene expression profiles were analyzed using microarray and validated using real-time RT-PCR and protein secretion studies. A total of 299 genes were differentially expressed, many of which are involved in immunity, signal transduction, transcription and apoptosis. Genes encoding the cytokines IL-12 and TNF-alpha, along with the chemokines CCL22, CCL27 and CXCL10, were up-regulated, suggesting that P. brasiliensis induces a potent proinflammatory response in DCs. in contrast, pattern recognition receptor (PRR)-encoding genes, particularly those related to Toll-like receptors, were down-regulated or unchanged. This result prompted us to evaluate the expression profiles of dectin-1 and mannose receptor, two other important fungal PRRs that were not included in the microarray target cDNA sequences. Unlike the mannose receptor, the dectin-1 receptor gene was significantly induced, suggesting that this beta-glucan receptor participates in the recognition of P. brasiliensis. We also used a receptor inhibition assay to evaluate the roles of these receptors in coordinating the expression of several immune-related genes in DCs upon fungal exposure. Altogether, our results provide an initial characterization of early host responses to P. brasiliensis and a basis for better understanding the infectious process of this important neglected pathogen.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
2016-01-24T14:17:38Z
2016-01-24T14:17:38Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pntd.0001459
Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 6, n. 1, 9 p., 2012.
10.1371/journal.pntd.0001459
WOS000300416100012.pdf
1935-2727
http://repositorio.unifesp.br/handle/11600/34393
WOS:000300416100012
url http://dx.doi.org/10.1371/journal.pntd.0001459
http://repositorio.unifesp.br/handle/11600/34393
identifier_str_mv Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 6, n. 1, 9 p., 2012.
10.1371/journal.pntd.0001459
WOS000300416100012.pdf
1935-2727
WOS:000300416100012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Plos Neglected Tropical Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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