Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/5748 http://dx.doi.org/10.1590/S1413-86702010000300023 |
Resumo: | Cytomegalovirus (CMV) antigenemia is still one of the two major assays available for diagnosis and monitoring of CMV infections. A commercial rapid test recently available in Brazil for quantification of human cytomegalovirus pp65 antigenemia revealed by immunofluorescence technique was compared with the original in-house method revealed by immunoperoxidase in patients receiving solid organ transplants. Of 80 blood samples tested for CMV antigenemia, 34 (42.5%) were positive: commercial assay detected 33 (97%) and in-house assay detected 20 (58.8%) samples. The numbers of positive cells in the two assays were different, with a median of 4.5 and 12 positive cells obtained by in-house and commercial kit, respectively. Discrepancies between assays occurred in 15 specimens from patients with low-grade antigenemia (median 6 positive cells). The assay-time was reduced in approximately 50% compared to in-house methodology. In conclusion, besides comparable results obtained for both assays, the commercial antigenemia assay provides more rapid and sensitive results. |
id |
UFSP_efbe1cfc31aec676241ca58084ce93cb |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/5748 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Carraro, Emerson [UNIFESP]Pasternak, Jacyr [UNIFESP]Perosa, Ana Helena Sitta [UNIFESP]Siqueira, Itacy [UNIFESP]Martino, Marines Dalla Vale [UNIFESP]Hospital Albert Einstein Clinical Laboratory Microbiology SectionUniversidade Federal de São Paulo (UNIFESP)2015-06-14T13:41:41Z2015-06-14T13:41:41Z2010-06-01Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 14, n. 3, p. 322-324, 2010.1413-8670http://repositorio.unifesp.br/handle/11600/5748http://dx.doi.org/10.1590/S1413-86702010000300023S1413-86702010000300023.pdfS1413-8670201000030002310.1590/S1413-86702010000300023WOS:000281693400023Cytomegalovirus (CMV) antigenemia is still one of the two major assays available for diagnosis and monitoring of CMV infections. A commercial rapid test recently available in Brazil for quantification of human cytomegalovirus pp65 antigenemia revealed by immunofluorescence technique was compared with the original in-house method revealed by immunoperoxidase in patients receiving solid organ transplants. Of 80 blood samples tested for CMV antigenemia, 34 (42.5%) were positive: commercial assay detected 33 (97%) and in-house assay detected 20 (58.8%) samples. The numbers of positive cells in the two assays were different, with a median of 4.5 and 12 positive cells obtained by in-house and commercial kit, respectively. Discrepancies between assays occurred in 15 specimens from patients with low-grade antigenemia (median 6 positive cells). The assay-time was reduced in approximately 50% compared to in-house methodology. In conclusion, besides comparable results obtained for both assays, the commercial antigenemia assay provides more rapid and sensitive results.Hospital Albert Einstein Clinical Laboratory Microbiology SectionUNIFESP Infectious Diseases Department Clinical Virology LaboratoryUNIFESP, Infectious Diseases Department Clinical Virology LaboratorySciELO322-324engBrazilian Society of Infectious DiseasesBrazilian Journal of Infectious Diseasescytomegalovirusantigenemia assayimmmunoperoxidaseimmunofluorescenceComparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS1413-86702010000300023.pdfapplication/pdf736166${dspace.ui.url}/bitstream/11600/5748/1/S1413-86702010000300023.pdfcb665939b0446b5fc007d5d2b23f2eacMD51open accessTEXTS1413-86702010000300023.pdf.txtS1413-86702010000300023.pdf.txtExtracted texttext/plain15689${dspace.ui.url}/bitstream/11600/5748/2/S1413-86702010000300023.pdf.txtde21602abad96298935ea6d1bb55efe3MD52open access11600/57482021-10-05 21:51:32.775open accessoai:repositorio.unifesp.br:11600/5748Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652021-10-06T00:51:32Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients |
title |
Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients |
spellingShingle |
Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients Carraro, Emerson [UNIFESP] cytomegalovirus antigenemia assay immmunoperoxidase immunofluorescence |
title_short |
Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients |
title_full |
Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients |
title_fullStr |
Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients |
title_full_unstemmed |
Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients |
title_sort |
Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients |
author |
Carraro, Emerson [UNIFESP] |
author_facet |
Carraro, Emerson [UNIFESP] Pasternak, Jacyr [UNIFESP] Perosa, Ana Helena Sitta [UNIFESP] Siqueira, Itacy [UNIFESP] Martino, Marines Dalla Vale [UNIFESP] |
author_role |
author |
author2 |
Pasternak, Jacyr [UNIFESP] Perosa, Ana Helena Sitta [UNIFESP] Siqueira, Itacy [UNIFESP] Martino, Marines Dalla Vale [UNIFESP] |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
Hospital Albert Einstein Clinical Laboratory Microbiology Section Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Carraro, Emerson [UNIFESP] Pasternak, Jacyr [UNIFESP] Perosa, Ana Helena Sitta [UNIFESP] Siqueira, Itacy [UNIFESP] Martino, Marines Dalla Vale [UNIFESP] |
dc.subject.eng.fl_str_mv |
cytomegalovirus antigenemia assay immmunoperoxidase immunofluorescence |
topic |
cytomegalovirus antigenemia assay immmunoperoxidase immunofluorescence |
description |
Cytomegalovirus (CMV) antigenemia is still one of the two major assays available for diagnosis and monitoring of CMV infections. A commercial rapid test recently available in Brazil for quantification of human cytomegalovirus pp65 antigenemia revealed by immunofluorescence technique was compared with the original in-house method revealed by immunoperoxidase in patients receiving solid organ transplants. Of 80 blood samples tested for CMV antigenemia, 34 (42.5%) were positive: commercial assay detected 33 (97%) and in-house assay detected 20 (58.8%) samples. The numbers of positive cells in the two assays were different, with a median of 4.5 and 12 positive cells obtained by in-house and commercial kit, respectively. Discrepancies between assays occurred in 15 specimens from patients with low-grade antigenemia (median 6 positive cells). The assay-time was reduced in approximately 50% compared to in-house methodology. In conclusion, besides comparable results obtained for both assays, the commercial antigenemia assay provides more rapid and sensitive results. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-06-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:41:41Z |
dc.date.available.fl_str_mv |
2015-06-14T13:41:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 14, n. 3, p. 322-324, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/5748 http://dx.doi.org/10.1590/S1413-86702010000300023 |
dc.identifier.issn.none.fl_str_mv |
1413-8670 |
dc.identifier.file.none.fl_str_mv |
S1413-86702010000300023.pdf |
dc.identifier.scielo.none.fl_str_mv |
S1413-86702010000300023 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1413-86702010000300023 |
dc.identifier.wos.none.fl_str_mv |
WOS:000281693400023 |
identifier_str_mv |
Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 14, n. 3, p. 322-324, 2010. 1413-8670 S1413-86702010000300023.pdf S1413-86702010000300023 10.1590/S1413-86702010000300023 WOS:000281693400023 |
url |
http://repositorio.unifesp.br/handle/11600/5748 http://dx.doi.org/10.1590/S1413-86702010000300023 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Brazilian Journal of Infectious Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
322-324 |
dc.publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
bitstream.url.fl_str_mv |
${dspace.ui.url}/bitstream/11600/5748/1/S1413-86702010000300023.pdf ${dspace.ui.url}/bitstream/11600/5748/2/S1413-86702010000300023.pdf.txt |
bitstream.checksum.fl_str_mv |
cb665939b0446b5fc007d5d2b23f2eac de21602abad96298935ea6d1bb55efe3 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764150211870720 |