Papel funcional das rocks sobre a proliferação de células de carcinoma epidermoide oral in vitro

Detalhes bibliográficos
Autor(a) principal: BORGES, Guilherme Henrique
Data de Publicação: 2017
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFTM
Texto Completo: http://bdtd.uftm.edu.br/handle/tede/781
Resumo: O carcinoma epidermoide oral é uma neoplasia epitelial responsável pela maioria das lesões malignas que acometem a região de cabeça e pescoço. O e o Y-27632 são inibidores das ROCKs, que são efetoras das GTPases Rho, e estão associadas à patogênese e progressão de tumores humanos. Este estudo visou avaliar o papel funcional das ROCKs na regulação da proliferação de células SCC-4 de carcinoma epidermoide oral in vitro. Foram quantificadas as células SCC-4 de carcinoma epidermoide oral controle e tratadas com HA-1077 (25, 50 e 100 μM) por 6 e 24 horas; e HA-1077 (25, 50 e 100 μM), HA-1077 50 µM e/ou Y-27632 30 µM por 6 horas, em ensaios de incorporação de BrdU e imunoexpressão de KI-67. Foi considerado significante p<0,05. O número de células SCC-4 tratadas com HA-1077 (25, 50 e 100 μM) que incorporaram BrdU foi significativamente menor que o de células controle em 6 horas [F(3,11) = 107,461, p<0,0001] e 24 horas [F(3,11) = 167,433, p<0,0001], de modo dose dependente. O número de células SCC-4 tratadas com HA-1077 (25, 50 e 100 μM), HA- 1077 50 µM e/ou Y-27632, por 6 horas, que incorporaram BrdU [F(5,17) = 443,818, p<0,0001], e o número que imunoexpressaram KI-67, também, foi significativamente menor [F(5,17) = 192,595, p<0,0001], que o de células controle. Os resultados obtidos sugerem que as proteínas ROCKs desempenham um papel funcional importante na regulação da proliferação celular em carcinoma epidermoide oral, uma vez que o HA-1077 e/ou o Y- 27632, inibidores de ROCKs, têm um efeito inibitório sobre a proliferação de células SCC-4.
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spelling Papel funcional das rocks sobre a proliferação de células de carcinoma epidermoide oral in vitroCarcinoma de epidermoide oral.HA-1077.Proliferação celular.ROCKs.Y-27632.Oral squamous cell carcinoma.HA-1077.Cell proliferation.ROCKs.Y-27632.MorfologiaCitologia e Biologia CelularO carcinoma epidermoide oral é uma neoplasia epitelial responsável pela maioria das lesões malignas que acometem a região de cabeça e pescoço. O e o Y-27632 são inibidores das ROCKs, que são efetoras das GTPases Rho, e estão associadas à patogênese e progressão de tumores humanos. Este estudo visou avaliar o papel funcional das ROCKs na regulação da proliferação de células SCC-4 de carcinoma epidermoide oral in vitro. Foram quantificadas as células SCC-4 de carcinoma epidermoide oral controle e tratadas com HA-1077 (25, 50 e 100 μM) por 6 e 24 horas; e HA-1077 (25, 50 e 100 μM), HA-1077 50 µM e/ou Y-27632 30 µM por 6 horas, em ensaios de incorporação de BrdU e imunoexpressão de KI-67. Foi considerado significante p<0,05. O número de células SCC-4 tratadas com HA-1077 (25, 50 e 100 μM) que incorporaram BrdU foi significativamente menor que o de células controle em 6 horas [F(3,11) = 107,461, p<0,0001] e 24 horas [F(3,11) = 167,433, p<0,0001], de modo dose dependente. O número de células SCC-4 tratadas com HA-1077 (25, 50 e 100 μM), HA- 1077 50 µM e/ou Y-27632, por 6 horas, que incorporaram BrdU [F(5,17) = 443,818, p<0,0001], e o número que imunoexpressaram KI-67, também, foi significativamente menor [F(5,17) = 192,595, p<0,0001], que o de células controle. Os resultados obtidos sugerem que as proteínas ROCKs desempenham um papel funcional importante na regulação da proliferação celular em carcinoma epidermoide oral, uma vez que o HA-1077 e/ou o Y- 27632, inibidores de ROCKs, têm um efeito inibitório sobre a proliferação de células SCC-4.The oral carcinoma cell squamous (OSCC) is epithelial cancer responsible for the majority of malignant lesions affecting the head and neck region. The HA-1077 and the Y-27632 are inhibitors of the ROCKs which are effector of Rho GTPases, and are associated with pathogenesis and progression of human tumors. This study aimed to evaluate the functional role of the ROCKs in the regulation of cellular proliferation of SCC-4 cell line from OSCC. Control cells and cells treated with HA-1077 (25, 50 and 100 μM) were quantified for 6 and 24 hours; and HA-1077 (25, 50 and 100 μM), HA-1077 50 µM and/or Y-27632 30 µM for 6 hours, in BrdU assays and immunoexpression of KI-67 assays. Was considered significant p<0.05. The number of SCC-4 cells treated with HA-1077 (25, 50 and 100 μM) that incorporated BrdU was significantly lower than control cells in 6 hours [F (3.11) = 107.461, p<0.0001] and 24 hours [F (3.11) = 167.433, p<0.0001], therefore dose dependent. The number of proliferative SCC-4 cells treated with HA-1077 (25, 50 and 100 μM), HA-1077 50 µM and/or Y-27632, for 6 hours, in BrdU assay [F (5.17) = 443.818, p<0.0001], and in imunoexpressaram KI-67 assay, also, was significantly less [F (5.17) = 192.595, p<0.0001], than control cells. The results obtained suggest that the ROCKs play an important functional role in the regulation of cellular proliferation in OSCC, since both HA-1077 and/or the Y- 27632, inhibitors of ROCKs, have an inhibitory effect on proliferation of SCC-4 cells.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorFundação de Amparo à Pesquisa do Estado de Minas GeraisUniversidade Federal do Triângulo MineiroUniversidade Federal do Triângulo MineiroInstituto de Ciências da Saúde - ICS::Programa de Pós-Graduação em Ciências da SaúdeBrasilUFTMPrograma de Pós-Graduação em Ciências da SaúdeCREMA, Virgínia Oliveira66127211620http://lattes.cnpq.br/2599388555203811BORGES, Guilherme Henrique2019-07-22T14:25:45Z2017-11-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfBORGES, Guilherme Henrique. Papel funcional das rocks sobre a proliferação de células de carcinoma epidermoide oral in vitro. 2017. 74f. 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dc.title.none.fl_str_mv Papel funcional das rocks sobre a proliferação de células de carcinoma epidermoide oral in vitro
title Papel funcional das rocks sobre a proliferação de células de carcinoma epidermoide oral in vitro
spellingShingle Papel funcional das rocks sobre a proliferação de células de carcinoma epidermoide oral in vitro
BORGES, Guilherme Henrique
Carcinoma de epidermoide oral.
HA-1077.
Proliferação celular.
ROCKs.
Y-27632.
Oral squamous cell carcinoma.
HA-1077.
Cell proliferation.
ROCKs.
Y-27632.
Morfologia
Citologia e Biologia Celular
title_short Papel funcional das rocks sobre a proliferação de células de carcinoma epidermoide oral in vitro
title_full Papel funcional das rocks sobre a proliferação de células de carcinoma epidermoide oral in vitro
title_fullStr Papel funcional das rocks sobre a proliferação de células de carcinoma epidermoide oral in vitro
title_full_unstemmed Papel funcional das rocks sobre a proliferação de células de carcinoma epidermoide oral in vitro
title_sort Papel funcional das rocks sobre a proliferação de células de carcinoma epidermoide oral in vitro
author BORGES, Guilherme Henrique
author_facet BORGES, Guilherme Henrique
author_role author
dc.contributor.none.fl_str_mv CREMA, Virgínia Oliveira
66127211620
http://lattes.cnpq.br/2599388555203811
dc.contributor.author.fl_str_mv BORGES, Guilherme Henrique
dc.subject.por.fl_str_mv Carcinoma de epidermoide oral.
HA-1077.
Proliferação celular.
ROCKs.
Y-27632.
Oral squamous cell carcinoma.
HA-1077.
Cell proliferation.
ROCKs.
Y-27632.
Morfologia
Citologia e Biologia Celular
topic Carcinoma de epidermoide oral.
HA-1077.
Proliferação celular.
ROCKs.
Y-27632.
Oral squamous cell carcinoma.
HA-1077.
Cell proliferation.
ROCKs.
Y-27632.
Morfologia
Citologia e Biologia Celular
description O carcinoma epidermoide oral é uma neoplasia epitelial responsável pela maioria das lesões malignas que acometem a região de cabeça e pescoço. O e o Y-27632 são inibidores das ROCKs, que são efetoras das GTPases Rho, e estão associadas à patogênese e progressão de tumores humanos. Este estudo visou avaliar o papel funcional das ROCKs na regulação da proliferação de células SCC-4 de carcinoma epidermoide oral in vitro. Foram quantificadas as células SCC-4 de carcinoma epidermoide oral controle e tratadas com HA-1077 (25, 50 e 100 μM) por 6 e 24 horas; e HA-1077 (25, 50 e 100 μM), HA-1077 50 µM e/ou Y-27632 30 µM por 6 horas, em ensaios de incorporação de BrdU e imunoexpressão de KI-67. Foi considerado significante p<0,05. O número de células SCC-4 tratadas com HA-1077 (25, 50 e 100 μM) que incorporaram BrdU foi significativamente menor que o de células controle em 6 horas [F(3,11) = 107,461, p<0,0001] e 24 horas [F(3,11) = 167,433, p<0,0001], de modo dose dependente. O número de células SCC-4 tratadas com HA-1077 (25, 50 e 100 μM), HA- 1077 50 µM e/ou Y-27632, por 6 horas, que incorporaram BrdU [F(5,17) = 443,818, p<0,0001], e o número que imunoexpressaram KI-67, também, foi significativamente menor [F(5,17) = 192,595, p<0,0001], que o de células controle. Os resultados obtidos sugerem que as proteínas ROCKs desempenham um papel funcional importante na regulação da proliferação celular em carcinoma epidermoide oral, uma vez que o HA-1077 e/ou o Y- 27632, inibidores de ROCKs, têm um efeito inibitório sobre a proliferação de células SCC-4.
publishDate 2017
dc.date.none.fl_str_mv 2017-11-22
2019-07-22T14:25:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv BORGES, Guilherme Henrique. Papel funcional das rocks sobre a proliferação de células de carcinoma epidermoide oral in vitro. 2017. 74f. Dissertação (Mestrado em Ciências da Saúde) - Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Triângulo Mineiro, Uberaba, 2017.
http://bdtd.uftm.edu.br/handle/tede/781
identifier_str_mv BORGES, Guilherme Henrique. Papel funcional das rocks sobre a proliferação de células de carcinoma epidermoide oral in vitro. 2017. 74f. Dissertação (Mestrado em Ciências da Saúde) - Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Triângulo Mineiro, Uberaba, 2017.
url http://bdtd.uftm.edu.br/handle/tede/781
dc.language.iso.fl_str_mv por
language por
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Brasil
UFTM
Programa de Pós-Graduação em Ciências da Saúde
publisher.none.fl_str_mv Universidade Federal do Triângulo Mineiro
Instituto de Ciências da Saúde - ICS::Programa de Pós-Graduação em Ciências da Saúde
Brasil
UFTM
Programa de Pós-Graduação em Ciências da Saúde
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