HA1077 inhibits cell proliferation of oral squamous cell carcinoma in vitro
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/26730 |
Resumo: | Oral squamous cell carcinoma is a common malignant lesion. ROCKs proteins are associated with pathogenesis and progression of human tumours. This study aimed to evaluate the functional role of ROCKs in the regulation of cell proliferation of oral squamous cell carcinoma in vitro. BrdU incorporation assays and KI-67 immunoexpression were performed by using SCC-4 cell line from oral squamous cell carcinoma. Control and treated cells: HA-1077 (25, 50 and 100 μmol/l), 50 μmol HA-1077 and Y-27632 30 μmol/l, Y-27632 30 μmol/l were cutured for 6 h. The number of SCC-4 cells treated with: HA-1077 (25, 50 and 100 μmol/l), HA-1077 50 μmol/l and/or Y-27632 30 μmol/l was significantly lower than control cells in BrdU assay [F (5.17) = 443.818, p<0.0001] and in KI-67 assay [F = 192.595, d.f. = 5,17; p<0.0001]. The results obtained suggest that the pathways that evolve ROCKs proteins play an important functional role in the positive regulation of cell proliferation in oral squamous cell carcinoma. |
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HA1077 inhibits cell proliferation of oral squamous cell carcinoma in vitroHA1077 inhibe la proliferación celular en el carcinoma oral de células escamosas in vitroHA1077 inibe a proliferação celular em carcinoma de células escamosas oral in vitroCell proliferationHA-1077Oral squamous cell carcinomaROCKsSCC-4Y-27632.Proliferação celularHA-1077Carcinoma de células escamosas oralROCKsSCC-4Y-27632.Proliferación celularHA-1077Carcinoma oral de células escamosasROCKsSCC-4Y-27632.Oral squamous cell carcinoma is a common malignant lesion. ROCKs proteins are associated with pathogenesis and progression of human tumours. This study aimed to evaluate the functional role of ROCKs in the regulation of cell proliferation of oral squamous cell carcinoma in vitro. BrdU incorporation assays and KI-67 immunoexpression were performed by using SCC-4 cell line from oral squamous cell carcinoma. Control and treated cells: HA-1077 (25, 50 and 100 μmol/l), 50 μmol HA-1077 and Y-27632 30 μmol/l, Y-27632 30 μmol/l were cutured for 6 h. The number of SCC-4 cells treated with: HA-1077 (25, 50 and 100 μmol/l), HA-1077 50 μmol/l and/or Y-27632 30 μmol/l was significantly lower than control cells in BrdU assay [F (5.17) = 443.818, p<0.0001] and in KI-67 assay [F = 192.595, d.f. = 5,17; p<0.0001]. The results obtained suggest that the pathways that evolve ROCKs proteins play an important functional role in the positive regulation of cell proliferation in oral squamous cell carcinoma.El carcinoma oral de células escamosas es una lesión maligna común. Las proteínas ROCKs están asociadas con la patogénesis y progresión de tumores humanos. Este estudio tuvo como objetivo evaluar el papel funcional de las ROCK en la regulación de la proliferación celular del carcinoma oral de células escamosas in vitro. Los ensayos de incorporación de BrdU y la inmunoexpresión de KI-67 se realizaron utilizando la línea celular SCC-4 de carcinoma de células escamosas orales. Células control y tratadas: HA-1077 (25, 50 y 100 μmol/l), 50 μmol HA-1077 y Y-27632 30 μmol/l, Y-27632 30 μmol/l se cultivaron durante 6 h. El número de células SCC-4 tratadas con: HA-1077 (25, 50 y 100 μmol/l), HA-1077 50 μmol/l y/o Y-27632 30 μmol/l fue significativamente menor que las células de control en el ensayo BrdU [F (5,17) = 443.818, p<0,0001] y en ensayo KI-67 [F = 192.595, df = 5,17; p<0,0001]. Los resultados obtenidos sugieren que las vías que evolucionan las proteínas ROCKs juegan un papel funcional importante en la regulación positiva de la proliferación celular en el carcinoma de células escamosas oral.Uma forma de tentar controlar o carcinoma de células escamosas oral é investir em novas terapias voltadas para a biologia molecular dos receptores e suas vias de sinalização intracelular. Este estudo teve como objetivo avaliar o efeito do LY2109761 (um inibidor dos receptores TGF-β) na migração celular no carcinoma epidermóide oral in vitro. Citoesqueleto de actina de controle de células SCC-4 e LY2109761 (1, 5 e 10 μM) tratado em Matrigel tridimensional foram analisados usando microscopia confocal a laser. Controle e células tratadas com LY2109761 (1, 5 e 10 μM) que migraram através da membrana de ensaios de migração de células tridimensionais foram contadas, a significância foi p <0,05. Células controle foram observadas com citoplasma volumoso, córtex celular preservado e citoesqueleto de actina bem desenvolvido com filamentos de actina bem distribuídos. Independentemente da concentração, as células tratadas apresentaram: morfologia arredondada e tamanho pequeno, citoplasma escasso, F-actina cortical menos clara que as células de controle e rompimento dos filamentos de actina. As células migratórias foram inibidas pelo tratamento com LY2109761 [F (3, 11) = 3742, p <0,0001], de uma forma dependente da dose. Esses resultados sugerem que o LY2109761 exerce um efeito inibitório sobre o citoesqueleto de actina e a migração celular nas células SCC-4, portanto, é uma opção terapêutica promissora para o carcinoma de células escamosas oral.Research, Society and Development2022-02-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2673010.33448/rsd-v11i3.26730Research, Society and Development; Vol. 11 No. 3; e36611326730Research, Society and Development; Vol. 11 Núm. 3; e36611326730Research, Society and Development; v. 11 n. 3; e366113267302525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/26730/23333Copyright (c) 2022 Guilherme Henrique Borges; Simone de Sales Costa Moreira Carboni; Anna Cecília Dias Maciel Carneiro; Lorraine Stephanie Hiss; Isadora Caixeta da Silveira; Virginia Oliveira Cremahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBorges, Guilherme HenriqueCarboni, Simone de Sales Costa Moreira Carneiro, Anna Cecília Dias Maciel Hiss, Lorraine Stephanie Silveira, Isadora Caixeta daCrema, Virginia Oliveira2022-03-09T13:44:38Zoai:ojs.pkp.sfu.ca:article/26730Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:44:41.931388Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
HA1077 inhibits cell proliferation of oral squamous cell carcinoma in vitro HA1077 inhibe la proliferación celular en el carcinoma oral de células escamosas in vitro HA1077 inibe a proliferação celular em carcinoma de células escamosas oral in vitro |
| title |
HA1077 inhibits cell proliferation of oral squamous cell carcinoma in vitro |
| spellingShingle |
HA1077 inhibits cell proliferation of oral squamous cell carcinoma in vitro Borges, Guilherme Henrique Cell proliferation HA-1077 Oral squamous cell carcinoma ROCKs SCC-4 Y-27632. Proliferação celular HA-1077 Carcinoma de células escamosas oral ROCKs SCC-4 Y-27632. Proliferación celular HA-1077 Carcinoma oral de células escamosas ROCKs SCC-4 Y-27632. |
| title_short |
HA1077 inhibits cell proliferation of oral squamous cell carcinoma in vitro |
| title_full |
HA1077 inhibits cell proliferation of oral squamous cell carcinoma in vitro |
| title_fullStr |
HA1077 inhibits cell proliferation of oral squamous cell carcinoma in vitro |
| title_full_unstemmed |
HA1077 inhibits cell proliferation of oral squamous cell carcinoma in vitro |
| title_sort |
HA1077 inhibits cell proliferation of oral squamous cell carcinoma in vitro |
| author |
Borges, Guilherme Henrique |
| author_facet |
Borges, Guilherme Henrique Carboni, Simone de Sales Costa Moreira Carneiro, Anna Cecília Dias Maciel Hiss, Lorraine Stephanie Silveira, Isadora Caixeta da Crema, Virginia Oliveira |
| author_role |
author |
| author2 |
Carboni, Simone de Sales Costa Moreira Carneiro, Anna Cecília Dias Maciel Hiss, Lorraine Stephanie Silveira, Isadora Caixeta da Crema, Virginia Oliveira |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Borges, Guilherme Henrique Carboni, Simone de Sales Costa Moreira Carneiro, Anna Cecília Dias Maciel Hiss, Lorraine Stephanie Silveira, Isadora Caixeta da Crema, Virginia Oliveira |
| dc.subject.por.fl_str_mv |
Cell proliferation HA-1077 Oral squamous cell carcinoma ROCKs SCC-4 Y-27632. Proliferação celular HA-1077 Carcinoma de células escamosas oral ROCKs SCC-4 Y-27632. Proliferación celular HA-1077 Carcinoma oral de células escamosas ROCKs SCC-4 Y-27632. |
| topic |
Cell proliferation HA-1077 Oral squamous cell carcinoma ROCKs SCC-4 Y-27632. Proliferação celular HA-1077 Carcinoma de células escamosas oral ROCKs SCC-4 Y-27632. Proliferación celular HA-1077 Carcinoma oral de células escamosas ROCKs SCC-4 Y-27632. |
| description |
Oral squamous cell carcinoma is a common malignant lesion. ROCKs proteins are associated with pathogenesis and progression of human tumours. This study aimed to evaluate the functional role of ROCKs in the regulation of cell proliferation of oral squamous cell carcinoma in vitro. BrdU incorporation assays and KI-67 immunoexpression were performed by using SCC-4 cell line from oral squamous cell carcinoma. Control and treated cells: HA-1077 (25, 50 and 100 μmol/l), 50 μmol HA-1077 and Y-27632 30 μmol/l, Y-27632 30 μmol/l were cutured for 6 h. The number of SCC-4 cells treated with: HA-1077 (25, 50 and 100 μmol/l), HA-1077 50 μmol/l and/or Y-27632 30 μmol/l was significantly lower than control cells in BrdU assay [F (5.17) = 443.818, p<0.0001] and in KI-67 assay [F = 192.595, d.f. = 5,17; p<0.0001]. The results obtained suggest that the pathways that evolve ROCKs proteins play an important functional role in the positive regulation of cell proliferation in oral squamous cell carcinoma. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-02-26 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/26730 10.33448/rsd-v11i3.26730 |
| url |
https://rsdjournal.org/index.php/rsd/article/view/26730 |
| identifier_str_mv |
10.33448/rsd-v11i3.26730 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/26730/23333 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Research, Society and Development |
| publisher.none.fl_str_mv |
Research, Society and Development |
| dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 3; e36611326730 Research, Society and Development; Vol. 11 Núm. 3; e36611326730 Research, Society and Development; v. 11 n. 3; e36611326730 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
| instname_str |
Universidade Federal de Itajubá (UNIFEI) |
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UNIFEI |
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UNIFEI |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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1797052706057093120 |