The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/42777 |
Resumo: | Angiogenesis is a fundamental physiological process with strong implications in tissue homeostasis. Animal models helping to identify how angiogenesis is regulated are fundamental to answer many biological questions. Chick embryo chorioallantoic membrane (CAM) assay is one of the most employed methods to study angiogenesis. In this study we applied a scientometric approach to evaluate the employment of CAM assay in published articles. Temporal trends indicated that CAM assay was the preferred method to investigate angiogenesis over time. The publications had a significant number of citations and the impact factor of journals publishing articles is relevant for the scientific community. A total of 52 different research areas have articles published using this particular technique. Oncology is the research field in which CAM assay was mostly used. Accordingly, tumor-derived cell lines were the most frequent sample tested on CAM. We also identified that 73,6% of articles published used only CAM assay to answer questions concerning angiogenesis. We concluded that although the CAM assay is a classical approach, that does not need so much infrastructure and financial support to be performed, it is a well-accepted technique by the scientific community. In addition, this methodology has gain attention in scientific community because no pain is experienced by the chick and they are minor ethical concerns to employ this method. Moreover, this data can help researchers who are unfamiliar with the CAM assay to identify if this particular method is suitable for their research. |
id |
UFU-14_0813fae98182b778257cd3c81b76d30f |
---|---|
oai_identifier_str |
oai:ojs.www.seer.ufu.br:article/42777 |
network_acronym_str |
UFU-14 |
network_name_str |
Bioscience journal (Online) |
repository_id_str |
|
spelling |
The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesisEnsaio da membrana corioalantóide de embrião de galinha como modelo para o estudo da angiogêneseAlternative methodangiogenesisbibliometric analysisCAM assayoncologytumor vascularization.Health sciencesAngiogenesis is a fundamental physiological process with strong implications in tissue homeostasis. Animal models helping to identify how angiogenesis is regulated are fundamental to answer many biological questions. Chick embryo chorioallantoic membrane (CAM) assay is one of the most employed methods to study angiogenesis. In this study we applied a scientometric approach to evaluate the employment of CAM assay in published articles. Temporal trends indicated that CAM assay was the preferred method to investigate angiogenesis over time. The publications had a significant number of citations and the impact factor of journals publishing articles is relevant for the scientific community. A total of 52 different research areas have articles published using this particular technique. Oncology is the research field in which CAM assay was mostly used. Accordingly, tumor-derived cell lines were the most frequent sample tested on CAM. We also identified that 73,6% of articles published used only CAM assay to answer questions concerning angiogenesis. We concluded that although the CAM assay is a classical approach, that does not need so much infrastructure and financial support to be performed, it is a well-accepted technique by the scientific community. In addition, this methodology has gain attention in scientific community because no pain is experienced by the chick and they are minor ethical concerns to employ this method. Moreover, this data can help researchers who are unfamiliar with the CAM assay to identify if this particular method is suitable for their research. A angiogênese é um processo fisiológico fundamental com fortes implicações na homeostase tecidual. Modelos animais que ajudam a entender como a angiogênese é regulada, são fundamentais para responder a muitas questões biológicas. O ensaio de membrana corioalantóide de embrião de galinha (CAM) é um dos métodos mais empregados para estudar a angiogênese. Neste estudo foi aplicada uma abordagem cientométrica para avaliar o emprego do ensaio CAM em artigos científicos já publicados. Tendências temporais indicaram que o ensaio CAM foi o método mais usado para investigar a angiogênese ao longo do tempo. Os artigos científicos que usaram a metodologia CAM foram publicados em periódicos com significativos números de citações e fator de impacto. No total 52 diferentes áreas de conhecimento usaram a técnica CAM, sendo a oncologia o campo o qual produziu maior número de artigos usando essa metodologia. Consequentemente o material biológico mais testado foi as linhagens celulares tumorais. Também foi identificado que 73,6% dos artigos publicados utilizaram apenas o teste CAM para responder questões relacionadas à angiogênese. Pode se concluir que embora o ensaio CAM seja uma abordagem clássica, que não necessita de muita infraestrutura e apoio financeiro para ser realizado, é uma técnica bem aceita pela comunidade científica. Além disso, esta metodologia tem ganhado atenção na comunidade científica porque os animais testados não sofrem dor e por essa razão esse modelo experimental exige mínimas preocupações éticas. Além disso, esses dados podem ajudar os pesquisadores que não estão familiarizados com o ensaio CAM a identificar se esse método específico é adequado para sua pesquisa.EDUFU2019-08-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/4277710.14393/BJ-v35n4a2019-42777Bioscience Journal ; Vol. 35 No. 4 (2019): July/Aug.; 1262-1275Bioscience Journal ; v. 35 n. 4 (2019): July/Aug.; 1262-12751981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/42777/26672Brazil; ContemporaryCopyright (c) 2019 Angélida Daiane Lemos do Prado, Elisa Flávia Luiz Cardoso Bailão, João Carlos Nabout, Tatiana Rabachini, Paulo Roberto de Melo Reis, Pablo José Gonçalves, Luciane Madureira Almeidahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessdo Prado, Angélida Daiane LemosBailão, Elisa Flávia Luiz CardosoNabout, João CarlosRabachini, TatianaMelo Reis, Paulo Roberto deGonçalves, Pablo José Almeida, Luciane Madureira2022-01-31T22:09:19Zoai:ojs.www.seer.ufu.br:article/42777Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-01-31T22:09:19Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis Ensaio da membrana corioalantóide de embrião de galinha como modelo para o estudo da angiogênese |
title |
The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis |
spellingShingle |
The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis do Prado, Angélida Daiane Lemos Alternative method angiogenesis bibliometric analysis CAM assay oncology tumor vascularization. Health sciences |
title_short |
The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis |
title_full |
The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis |
title_fullStr |
The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis |
title_full_unstemmed |
The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis |
title_sort |
The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis |
author |
do Prado, Angélida Daiane Lemos |
author_facet |
do Prado, Angélida Daiane Lemos Bailão, Elisa Flávia Luiz Cardoso Nabout, João Carlos Rabachini, Tatiana Melo Reis, Paulo Roberto de Gonçalves, Pablo José Almeida, Luciane Madureira |
author_role |
author |
author2 |
Bailão, Elisa Flávia Luiz Cardoso Nabout, João Carlos Rabachini, Tatiana Melo Reis, Paulo Roberto de Gonçalves, Pablo José Almeida, Luciane Madureira |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
do Prado, Angélida Daiane Lemos Bailão, Elisa Flávia Luiz Cardoso Nabout, João Carlos Rabachini, Tatiana Melo Reis, Paulo Roberto de Gonçalves, Pablo José Almeida, Luciane Madureira |
dc.subject.por.fl_str_mv |
Alternative method angiogenesis bibliometric analysis CAM assay oncology tumor vascularization. Health sciences |
topic |
Alternative method angiogenesis bibliometric analysis CAM assay oncology tumor vascularization. Health sciences |
description |
Angiogenesis is a fundamental physiological process with strong implications in tissue homeostasis. Animal models helping to identify how angiogenesis is regulated are fundamental to answer many biological questions. Chick embryo chorioallantoic membrane (CAM) assay is one of the most employed methods to study angiogenesis. In this study we applied a scientometric approach to evaluate the employment of CAM assay in published articles. Temporal trends indicated that CAM assay was the preferred method to investigate angiogenesis over time. The publications had a significant number of citations and the impact factor of journals publishing articles is relevant for the scientific community. A total of 52 different research areas have articles published using this particular technique. Oncology is the research field in which CAM assay was mostly used. Accordingly, tumor-derived cell lines were the most frequent sample tested on CAM. We also identified that 73,6% of articles published used only CAM assay to answer questions concerning angiogenesis. We concluded that although the CAM assay is a classical approach, that does not need so much infrastructure and financial support to be performed, it is a well-accepted technique by the scientific community. In addition, this methodology has gain attention in scientific community because no pain is experienced by the chick and they are minor ethical concerns to employ this method. Moreover, this data can help researchers who are unfamiliar with the CAM assay to identify if this particular method is suitable for their research. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08-08 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/42777 10.14393/BJ-v35n4a2019-42777 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/42777 |
identifier_str_mv |
10.14393/BJ-v35n4a2019-42777 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/42777/26672 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 35 No. 4 (2019): July/Aug.; 1262-1275 Bioscience Journal ; v. 35 n. 4 (2019): July/Aug.; 1262-1275 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069080954404864 |