The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis

Detalhes bibliográficos
Autor(a) principal: do Prado, Angélida Daiane Lemos
Data de Publicação: 2019
Outros Autores: Bailão, Elisa Flávia Luiz Cardoso, Nabout, João Carlos, Rabachini, Tatiana, Melo Reis, Paulo Roberto de, Gonçalves, Pablo José, Almeida, Luciane Madureira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/42777
Resumo: Angiogenesis is a fundamental physiological process with strong implications in tissue homeostasis. Animal models helping to identify how angiogenesis is regulated are fundamental to answer many biological questions. Chick embryo chorioallantoic membrane (CAM) assay is one of the most employed methods to study angiogenesis. In this study we applied a scientometric approach to evaluate the employment of CAM assay in published articles. Temporal trends indicated that CAM assay was the preferred method to investigate angiogenesis over time. The publications had a significant number of citations and the impact factor of journals publishing articles is relevant for the scientific community. A total of 52 different research areas have articles published using this particular technique. Oncology is the research field in which CAM assay was mostly used. Accordingly, tumor-derived cell lines were the most frequent sample tested on CAM. We also identified that 73,6% of articles published used only CAM assay to answer questions concerning angiogenesis. We concluded that although the CAM assay is a classical approach, that does not need so much infrastructure and financial support to be performed, it is a well-accepted technique by the scientific community. In addition, this methodology has gain attention in scientific community because no pain is experienced by the chick and they are minor ethical concerns to employ this method. Moreover, this data can help researchers who are unfamiliar with the CAM assay to identify if this particular method is suitable for their research.  
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spelling The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesisEnsaio da membrana corioalantóide de embrião de galinha como modelo para o estudo da angiogêneseAlternative methodangiogenesisbibliometric analysisCAM assayoncologytumor vascularization.Health sciencesAngiogenesis is a fundamental physiological process with strong implications in tissue homeostasis. Animal models helping to identify how angiogenesis is regulated are fundamental to answer many biological questions. Chick embryo chorioallantoic membrane (CAM) assay is one of the most employed methods to study angiogenesis. In this study we applied a scientometric approach to evaluate the employment of CAM assay in published articles. Temporal trends indicated that CAM assay was the preferred method to investigate angiogenesis over time. The publications had a significant number of citations and the impact factor of journals publishing articles is relevant for the scientific community. A total of 52 different research areas have articles published using this particular technique. Oncology is the research field in which CAM assay was mostly used. Accordingly, tumor-derived cell lines were the most frequent sample tested on CAM. We also identified that 73,6% of articles published used only CAM assay to answer questions concerning angiogenesis. We concluded that although the CAM assay is a classical approach, that does not need so much infrastructure and financial support to be performed, it is a well-accepted technique by the scientific community. In addition, this methodology has gain attention in scientific community because no pain is experienced by the chick and they are minor ethical concerns to employ this method. Moreover, this data can help researchers who are unfamiliar with the CAM assay to identify if this particular method is suitable for their research.  A angiogênese é um processo fisiológico fundamental com fortes implicações na homeostase tecidual. Modelos animais que ajudam a entender como a angiogênese é regulada, são fundamentais para responder a muitas questões biológicas. O ensaio de membrana corioalantóide de embrião de galinha (CAM) é um dos métodos mais empregados para estudar a angiogênese. Neste estudo foi aplicada uma abordagem cientométrica para avaliar o emprego do ensaio CAM em artigos científicos já publicados. Tendências temporais indicaram que o ensaio CAM foi o método mais usado para investigar a angiogênese ao longo do tempo. Os artigos científicos que usaram a metodologia CAM foram publicados em periódicos com significativos números de citações e fator de impacto. No total 52 diferentes áreas de conhecimento usaram a técnica CAM, sendo a oncologia o campo o qual produziu maior número de artigos usando essa metodologia. Consequentemente o material biológico mais testado foi as linhagens celulares tumorais. Também foi identificado que 73,6% dos artigos publicados utilizaram apenas o teste CAM para responder questões relacionadas à angiogênese. Pode se concluir que embora o ensaio CAM seja uma abordagem clássica, que não necessita de muita infraestrutura e apoio financeiro para ser realizado, é uma técnica bem aceita pela comunidade científica. Além disso, esta metodologia tem ganhado atenção na comunidade científica porque os animais testados não sofrem dor e por essa razão esse modelo experimental exige mínimas preocupações éticas. Além disso, esses dados podem ajudar os pesquisadores que não estão familiarizados com o ensaio CAM a identificar se esse método específico é adequado para sua pesquisa.EDUFU2019-08-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/4277710.14393/BJ-v35n4a2019-42777Bioscience Journal ; Vol. 35 No. 4 (2019): July/Aug.; 1262-1275Bioscience Journal ; v. 35 n. 4 (2019): July/Aug.; 1262-12751981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/42777/26672Brazil; ContemporaryCopyright (c) 2019 Angélida Daiane Lemos do Prado, Elisa Flávia Luiz Cardoso Bailão, João Carlos Nabout, Tatiana Rabachini, Paulo Roberto de Melo Reis, Pablo José Gonçalves, Luciane Madureira Almeidahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessdo Prado, Angélida Daiane LemosBailão, Elisa Flávia Luiz CardosoNabout, João CarlosRabachini, TatianaMelo Reis, Paulo Roberto deGonçalves, Pablo José Almeida, Luciane Madureira2022-01-31T22:09:19Zoai:ojs.www.seer.ufu.br:article/42777Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-01-31T22:09:19Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis
Ensaio da membrana corioalantóide de embrião de galinha como modelo para o estudo da angiogênese
title The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis
spellingShingle The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis
do Prado, Angélida Daiane Lemos
Alternative method
angiogenesis
bibliometric analysis
CAM assay
oncology
tumor vascularization.
Health sciences
title_short The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis
title_full The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis
title_fullStr The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis
title_full_unstemmed The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis
title_sort The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis
author do Prado, Angélida Daiane Lemos
author_facet do Prado, Angélida Daiane Lemos
Bailão, Elisa Flávia Luiz Cardoso
Nabout, João Carlos
Rabachini, Tatiana
Melo Reis, Paulo Roberto de
Gonçalves, Pablo José
Almeida, Luciane Madureira
author_role author
author2 Bailão, Elisa Flávia Luiz Cardoso
Nabout, João Carlos
Rabachini, Tatiana
Melo Reis, Paulo Roberto de
Gonçalves, Pablo José
Almeida, Luciane Madureira
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv do Prado, Angélida Daiane Lemos
Bailão, Elisa Flávia Luiz Cardoso
Nabout, João Carlos
Rabachini, Tatiana
Melo Reis, Paulo Roberto de
Gonçalves, Pablo José
Almeida, Luciane Madureira
dc.subject.por.fl_str_mv Alternative method
angiogenesis
bibliometric analysis
CAM assay
oncology
tumor vascularization.
Health sciences
topic Alternative method
angiogenesis
bibliometric analysis
CAM assay
oncology
tumor vascularization.
Health sciences
description Angiogenesis is a fundamental physiological process with strong implications in tissue homeostasis. Animal models helping to identify how angiogenesis is regulated are fundamental to answer many biological questions. Chick embryo chorioallantoic membrane (CAM) assay is one of the most employed methods to study angiogenesis. In this study we applied a scientometric approach to evaluate the employment of CAM assay in published articles. Temporal trends indicated that CAM assay was the preferred method to investigate angiogenesis over time. The publications had a significant number of citations and the impact factor of journals publishing articles is relevant for the scientific community. A total of 52 different research areas have articles published using this particular technique. Oncology is the research field in which CAM assay was mostly used. Accordingly, tumor-derived cell lines were the most frequent sample tested on CAM. We also identified that 73,6% of articles published used only CAM assay to answer questions concerning angiogenesis. We concluded that although the CAM assay is a classical approach, that does not need so much infrastructure and financial support to be performed, it is a well-accepted technique by the scientific community. In addition, this methodology has gain attention in scientific community because no pain is experienced by the chick and they are minor ethical concerns to employ this method. Moreover, this data can help researchers who are unfamiliar with the CAM assay to identify if this particular method is suitable for their research.  
publishDate 2019
dc.date.none.fl_str_mv 2019-08-08
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/42777
10.14393/BJ-v35n4a2019-42777
url https://seer.ufu.br/index.php/biosciencejournal/article/view/42777
identifier_str_mv 10.14393/BJ-v35n4a2019-42777
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/42777/26672
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Brazil; Contemporary
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 35 No. 4 (2019): July/Aug.; 1262-1275
Bioscience Journal ; v. 35 n. 4 (2019): July/Aug.; 1262-1275
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
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