Chemical constituents and in vivo preliminary evaluation of the toxicological activity of Ouratea spectabilis (OCHNACEAE) and Clitoria guianensis (Fabaceae) leaves

Detalhes bibliográficos
Autor(a) principal: Sousa, Frank Bruno Vieira de
Data de Publicação: 2023
Outros Autores: Cruz, Állefe Barbosa, Soares, Daniela Francisca, Cunha, Camila Luiza, Holzbach, Juliana C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/62323
Resumo: Clitoria guianensis and Ouratea spectabilis, found in the Brazilian Cerrado, are used in folk medicine, despite the few chemical and biological studies reported in the literature. The present study aims to investigate the toxicity and effect of extracts from both species on the microcrustacean Artemia salina, and to determine the chemical composition of the hexane extract of O. spectabilis leaves and the EtOAc fraction of C. guianensis leaves. Kaempferitrin, a flavonoid isolated from of the EtOAc fraction of C. guianensis leaves, was identified by chemical analysis. Analysis of the hexane extract of O. spectabilis leaves using gas chromatography-mass spectrometry (GC-MS) suggested the presence of twenty-five known substances. The Hex, EtOAc, and EtOH crude extracts of C. guianensis leaves exhibited high and moderate toxicity against Artemia salina, with median lethal dose values (LD50) of 43.7, 25.4, and 233.4 mg.L−1, respectively. The acetone extract of O. spectabilis leaves showed moderate toxicity against Artemia salina with an LD50 value of 115.13 mg.L−1.
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spelling Chemical constituents and in vivo preliminary evaluation of the toxicological activity of Ouratea spectabilis (OCHNACEAE) and Clitoria guianensis (Fabaceae) leavesArtemia salinaCerradoKaempferitrin.Biological SciencesClitoria guianensis and Ouratea spectabilis, found in the Brazilian Cerrado, are used in folk medicine, despite the few chemical and biological studies reported in the literature. The present study aims to investigate the toxicity and effect of extracts from both species on the microcrustacean Artemia salina, and to determine the chemical composition of the hexane extract of O. spectabilis leaves and the EtOAc fraction of C. guianensis leaves. Kaempferitrin, a flavonoid isolated from of the EtOAc fraction of C. guianensis leaves, was identified by chemical analysis. Analysis of the hexane extract of O. spectabilis leaves using gas chromatography-mass spectrometry (GC-MS) suggested the presence of twenty-five known substances. The Hex, EtOAc, and EtOH crude extracts of C. guianensis leaves exhibited high and moderate toxicity against Artemia salina, with median lethal dose values (LD50) of 43.7, 25.4, and 233.4 mg.L−1, respectively. The acetone extract of O. spectabilis leaves showed moderate toxicity against Artemia salina with an LD50 value of 115.13 mg.L−1.As espécies Clitoria guianensis e Ouratea spectabilis, encontradas no Cerrado brasileiro, são utilizadas na medicina popular, apesar de não apresentarem estudos químicos e biológicos reportados na literatura. O presente trabalho busca investigar a toxicidade frente ao microcrustáceo Artemia salina, dos extratos destas espécies, assim como a composição química do extrato hexânico das folhas de O. spectabilis e da fração EtOAc das folhas de C. guianensis. O estudo químico da fração EtOAc das folhas de C. guianensis resultou no isolamento de um flavonoide conhecido como Kaempferitrin. A análise do extrato hexânico das folhas de O. spectabilis, por CG-EM, sugere a presença de 13 substâncias conhecidas. Os extratos Hex, EtOAc e EtOH bruto de C. guianensis mostraram alta e moderada toxicidade frente a Artemia salina, respectivamente, com valores de dose letal (DL50) de 43.7, 25.4 e 233.4 mg.L-1. O extrato acetônico das folhas de O. spectabilis apresentou moderada toxicidade frente a Artemia salina com DL50 de 114.8 mg.L-1.Universidade Federal de Uberlândia2023-01-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/6232310.14393/BJ-v39n0a2023-62323Bioscience Journal ; Vol. 39 (2023): Continuous Publication; e39010Bioscience Journal ; v. 39 (2023): Continuous Publication; e390101981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/62323/35500Brazil; Contemporary Copyright (c) 2023 Frank Bruno Vieira de Sousa, Állefe Barbosa Cruz, Daniela Francisca Soares, Camila Luiza Cunha, Juliana C. Holzbachhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSousa, Frank Bruno Vieira deCruz, Állefe BarbosaSoares, Daniela FranciscaCunha, Camila LuizaHolzbach, Juliana C.2024-01-31T19:16:18Zoai:ojs.www.seer.ufu.br:article/62323Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2024-01-31T19:16:18Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Chemical constituents and in vivo preliminary evaluation of the toxicological activity of Ouratea spectabilis (OCHNACEAE) and Clitoria guianensis (Fabaceae) leaves
title Chemical constituents and in vivo preliminary evaluation of the toxicological activity of Ouratea spectabilis (OCHNACEAE) and Clitoria guianensis (Fabaceae) leaves
spellingShingle Chemical constituents and in vivo preliminary evaluation of the toxicological activity of Ouratea spectabilis (OCHNACEAE) and Clitoria guianensis (Fabaceae) leaves
Sousa, Frank Bruno Vieira de
Artemia salina
Cerrado
Kaempferitrin.
Biological Sciences
title_short Chemical constituents and in vivo preliminary evaluation of the toxicological activity of Ouratea spectabilis (OCHNACEAE) and Clitoria guianensis (Fabaceae) leaves
title_full Chemical constituents and in vivo preliminary evaluation of the toxicological activity of Ouratea spectabilis (OCHNACEAE) and Clitoria guianensis (Fabaceae) leaves
title_fullStr Chemical constituents and in vivo preliminary evaluation of the toxicological activity of Ouratea spectabilis (OCHNACEAE) and Clitoria guianensis (Fabaceae) leaves
title_full_unstemmed Chemical constituents and in vivo preliminary evaluation of the toxicological activity of Ouratea spectabilis (OCHNACEAE) and Clitoria guianensis (Fabaceae) leaves
title_sort Chemical constituents and in vivo preliminary evaluation of the toxicological activity of Ouratea spectabilis (OCHNACEAE) and Clitoria guianensis (Fabaceae) leaves
author Sousa, Frank Bruno Vieira de
author_facet Sousa, Frank Bruno Vieira de
Cruz, Állefe Barbosa
Soares, Daniela Francisca
Cunha, Camila Luiza
Holzbach, Juliana C.
author_role author
author2 Cruz, Állefe Barbosa
Soares, Daniela Francisca
Cunha, Camila Luiza
Holzbach, Juliana C.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Sousa, Frank Bruno Vieira de
Cruz, Állefe Barbosa
Soares, Daniela Francisca
Cunha, Camila Luiza
Holzbach, Juliana C.
dc.subject.por.fl_str_mv Artemia salina
Cerrado
Kaempferitrin.
Biological Sciences
topic Artemia salina
Cerrado
Kaempferitrin.
Biological Sciences
description Clitoria guianensis and Ouratea spectabilis, found in the Brazilian Cerrado, are used in folk medicine, despite the few chemical and biological studies reported in the literature. The present study aims to investigate the toxicity and effect of extracts from both species on the microcrustacean Artemia salina, and to determine the chemical composition of the hexane extract of O. spectabilis leaves and the EtOAc fraction of C. guianensis leaves. Kaempferitrin, a flavonoid isolated from of the EtOAc fraction of C. guianensis leaves, was identified by chemical analysis. Analysis of the hexane extract of O. spectabilis leaves using gas chromatography-mass spectrometry (GC-MS) suggested the presence of twenty-five known substances. The Hex, EtOAc, and EtOH crude extracts of C. guianensis leaves exhibited high and moderate toxicity against Artemia salina, with median lethal dose values (LD50) of 43.7, 25.4, and 233.4 mg.L−1, respectively. The acetone extract of O. spectabilis leaves showed moderate toxicity against Artemia salina with an LD50 value of 115.13 mg.L−1.
publishDate 2023
dc.date.none.fl_str_mv 2023-01-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/62323
10.14393/BJ-v39n0a2023-62323
url https://seer.ufu.br/index.php/biosciencejournal/article/view/62323
identifier_str_mv 10.14393/BJ-v39n0a2023-62323
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/62323/35500
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Brazil; Contemporary
dc.publisher.none.fl_str_mv Universidade Federal de Uberlândia
publisher.none.fl_str_mv Universidade Federal de Uberlândia
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 39 (2023): Continuous Publication; e39010
Bioscience Journal ; v. 39 (2023): Continuous Publication; e39010
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
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