Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk

Detalhes bibliográficos
Autor(a) principal: Vanetti, Maria C.D.
Data de Publicação: 2014
Outros Autores: Martins, Maurilio L., Pinto, Uelinton, M., Riedel, Kathrin, Mantovani, Hilário C., Araújo, Elza F. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/S1517-83822014000300037
http://www.locus.ufv.br/handle/123456789/13153
Resumo: Numerous bacteria coordinate gene expression in response to small signalling molecules in many cases known as acylhomoserine lactones (AHLs), which accumulate as a function of cell density in a process known as quorum sensing. This work aimed to determine if phenotypes that are important to define microbial activity in foods such as biofilm formation, swarming motility and proteolytic activity of two Pseudomonas fluorescens strains, isolated from refrigerated raw milk, are influenced by AHL molecules. The tested P. fluorescens strains did not produce AHL molecules in none of the evaluated media. We found that biofilm formation was dependent on the culture media, but it was not influenced by AHLs. Our results indicate that biofilm formation, swarming motility and proteolytic activity of the tested P. fluorescens strains are not regulated by acyl-homoserine lactones. It is likely that AHL-dependent quorum sensing system is absent from these strains
id UFV_19a29d2a11906a928001bafdb98c8dd7
oai_identifier_str oai:locus.ufv.br:123456789/13153
network_acronym_str UFV
network_name_str LOCUS Repositório Institucional da UFV
repository_id_str 2145
spelling Vanetti, Maria C.D.Martins, Maurilio L.Pinto, Uelinton, M.Riedel, KathrinMantovani, Hilário C.Araújo, Elza F. de2017-11-16T17:24:24Z2017-11-16T17:24:24Z2014-10-0916784405http://dx.doi.org/10.1590/S1517-83822014000300037http://www.locus.ufv.br/handle/123456789/13153Numerous bacteria coordinate gene expression in response to small signalling molecules in many cases known as acylhomoserine lactones (AHLs), which accumulate as a function of cell density in a process known as quorum sensing. This work aimed to determine if phenotypes that are important to define microbial activity in foods such as biofilm formation, swarming motility and proteolytic activity of two Pseudomonas fluorescens strains, isolated from refrigerated raw milk, are influenced by AHL molecules. The tested P. fluorescens strains did not produce AHL molecules in none of the evaluated media. We found that biofilm formation was dependent on the culture media, but it was not influenced by AHLs. Our results indicate that biofilm formation, swarming motility and proteolytic activity of the tested P. fluorescens strains are not regulated by acyl-homoserine lactones. It is likely that AHL-dependent quorum sensing system is absent from these strainsengBrazilian Journal of Microbiology45: 3, p. 1039–1046, Oct. 2014MilkPseudomonas fluorescensBiofilm formationQuorum sensingQuorum quenchingLack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milkinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALbjm-45-1039.pdfbjm-45-1039.pdftexto completoapplication/pdf906584https://locus.ufv.br//bitstream/123456789/13153/1/bjm-45-1039.pdfab9b64962ce3ebe972262dd2932bb2a4MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/13153/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILbjm-45-1039.pdf.jpgbjm-45-1039.pdf.jpgIM Thumbnailimage/jpeg4975https://locus.ufv.br//bitstream/123456789/13153/3/bjm-45-1039.pdf.jpgda1252b2d815f467d0ebfdf4efed5c94MD53123456789/131532017-11-16 22:00:25.011oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-11-17T01:00:25LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk
title Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk
spellingShingle Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk
Vanetti, Maria C.D.
Milk
Pseudomonas fluorescens
Biofilm formation
Quorum sensing
Quorum quenching
title_short Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk
title_full Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk
title_fullStr Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk
title_full_unstemmed Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk
title_sort Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk
author Vanetti, Maria C.D.
author_facet Vanetti, Maria C.D.
Martins, Maurilio L.
Pinto, Uelinton, M.
Riedel, Kathrin
Mantovani, Hilário C.
Araújo, Elza F. de
author_role author
author2 Martins, Maurilio L.
Pinto, Uelinton, M.
Riedel, Kathrin
Mantovani, Hilário C.
Araújo, Elza F. de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Vanetti, Maria C.D.
Martins, Maurilio L.
Pinto, Uelinton, M.
Riedel, Kathrin
Mantovani, Hilário C.
Araújo, Elza F. de
dc.subject.pt-BR.fl_str_mv Milk
Pseudomonas fluorescens
Biofilm formation
Quorum sensing
Quorum quenching
topic Milk
Pseudomonas fluorescens
Biofilm formation
Quorum sensing
Quorum quenching
description Numerous bacteria coordinate gene expression in response to small signalling molecules in many cases known as acylhomoserine lactones (AHLs), which accumulate as a function of cell density in a process known as quorum sensing. This work aimed to determine if phenotypes that are important to define microbial activity in foods such as biofilm formation, swarming motility and proteolytic activity of two Pseudomonas fluorescens strains, isolated from refrigerated raw milk, are influenced by AHL molecules. The tested P. fluorescens strains did not produce AHL molecules in none of the evaluated media. We found that biofilm formation was dependent on the culture media, but it was not influenced by AHLs. Our results indicate that biofilm formation, swarming motility and proteolytic activity of the tested P. fluorescens strains are not regulated by acyl-homoserine lactones. It is likely that AHL-dependent quorum sensing system is absent from these strains
publishDate 2014
dc.date.issued.fl_str_mv 2014-10-09
dc.date.accessioned.fl_str_mv 2017-11-16T17:24:24Z
dc.date.available.fl_str_mv 2017-11-16T17:24:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1517-83822014000300037
http://www.locus.ufv.br/handle/123456789/13153
dc.identifier.issn.none.fl_str_mv 16784405
identifier_str_mv 16784405
url http://dx.doi.org/10.1590/S1517-83822014000300037
http://www.locus.ufv.br/handle/123456789/13153
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv 45: 3, p. 1039–1046, Oct. 2014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Brazilian Journal of Microbiology
publisher.none.fl_str_mv Brazilian Journal of Microbiology
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
bitstream.url.fl_str_mv https://locus.ufv.br//bitstream/123456789/13153/1/bjm-45-1039.pdf
https://locus.ufv.br//bitstream/123456789/13153/2/license.txt
https://locus.ufv.br//bitstream/123456789/13153/3/bjm-45-1039.pdf.jpg
bitstream.checksum.fl_str_mv ab9b64962ce3ebe972262dd2932bb2a4
8a4605be74aa9ea9d79846c1fba20a33
da1252b2d815f467d0ebfdf4efed5c94
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
_version_ 1801213094023659520