Influência da concentração do EDTA, tempo e temperatura de armazenagem sobre parâmetros hematológicos de cães no hemograma automatizado e manual

• Autor(a) principal: Oliveira, Aécio Carlos de
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/4986
• topic: Sangue; Cão; Hemograma; Blood; Dog; Count blood; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL

The study aimed to identify the effects of time, temperature of storage and excess of anticoagulant on hematological parameters of dogs. Blood samples of ten, clinically healthy dogs, of different breeds were utilized. Aliquots were taken with 1.8 mg, 3.6 mg, 7.2 mg and 14.4 mg of ethylenediaminetetraacetic acid (EDTA) per mL of blood, divided into two groups: 2°C to 8ºC and room temperature. Right after collection, they were evaluated in four times: 0h, 12h, 24h and 48 h. White blood cells, red blood cells, hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red cell distribution width (RDW) platelet, thrombocrit (PCT), mean platelet volume (MPV) and platelet distribution width (PDW) were evaluated in the automatic cell counter. Hematocrit was determined using the microhematocrit technique, simultaneously with the analysis carried out in the automatic blood cell counter. The hemoglobin determination in the spectrophotometer, the total leukocyte count in the Neubauer chamber, the leukocyte count by microscopy of stained smears and the plasma protein dosage by refractometry were also done. In automatic cell counter analysis, the MCV increased significantly in higher concentrations of EDTA (7.2 mg / ml and 14.4 mg / mL) peaking at2.36%, in the highest concentration. Temperature and time also influenced the MCV, which showed a decrease in time 12 hours, in the temperature of 2 º C to 8 º C and an increase in time 24 hours and 48 hours at room temperature. MCH showed minor changes related to time and temperature and MCHC, related to time, temperature and EDTA. MPV and PDW showed a slight increase over time. Storage temperature influenced slightly in the leukocytes anderythrocytes counts, which showed lower values at room temperature. Hemoglobin, hematocrit, platelet and PCT did not change significantly. The changes observed did not compromise the results obtained automatic cell counter, showing that the blood samples stored for two days remained in good condition for processing, especially when stored under refrigeration. In the data obtained without automation, the hematocrit decreased significantly with the increasing concentration of EDTA: in the highest concentration (14.4 mg of EDTA per mL of blood), it showed an average decrease of 25.35%. The time also affected the hematocrit, which increased (p<0.05) in 48 hours time. The time factor has positively influenced the protein, which had an average increase of 11% in 48 hours time. The protein was also influenced by the concentration of EDTA, with considerable increase in the average value, in the concentration of 14.4 mg/mL of blood. In the differential count, made by microscopy, small changes in the number of neutrophils and monocytes were detected, related to time and the concentration of EDTA. Rods counting suffered the most significant changes related to time and temperature. WBC, hemoglobin, lymphocytes, eosinophils and basophils did not present relevant changes. The hematocrit decrease in the samples with high concentration of EDTA demonstrated the importance of the blood / EDTA proportion to the quality of the blood count, using the microhematocrit technique.