Diversidade e estrutura genética de begomovírus em duas regiões produtoras de tomate do Sudeste do Brasil

Detalhes bibliográficos
Autor(a) principal: Urquiza, Gloria Patricia Castillo
Data de Publicação: 2008
Tipo de documento: Tese
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/1021
Resumo: The incidence of begomoviruses has sharply increased in Brazil since the mid 1990 s, after the introduction of the B biotype of the whitefly Bemisia tabaci. It is believed that the insect vector transferred indigenous viruses infecting wild and weed hosts to tomato. After a rapid evolutionary process, novel species adapted to the new host became prevalent in the field. To date, five begomovirus species infecting tomatoes have been characterized, and six tentative species have been partially characterized. The objectives of this work were to determine the diversity and the genetic structure of begomovirus populations infecting tomatoes and associated weeds in two major tomato growing regions of Southeastern Brazil, the cities of Paty do Alferes (Rio de Janeiro state) and Coimbra (Minas Gerais state). The genetic structure of populations refers to the degree of genetic variability and its distribution within and between subpopulations, reflecting the evolutionary history and the potential of the population to evolve. A total of 119 tomato samples were collected in Paty do Alferes in May 2005. In Coimbra, 17 tomato samples and 43 weed samples were collected in July 2007. Total DNA was extracted from the samples and full-length begomovirus genomes were cloned by rolling circle amplification. Clones were completely sequenced and the sequences were used for comparisons with previously described begomoviruses, for phylogenetic analysis and for the determination of the genetic structure of viral populations. Sequence comparisons indicated the presence of two novel begomovirus species in tomato plants collected in Paty do Alferes, three novel species in weed samples collected in Coimbra, and one novel species in tomato samples collected at both locations. Sequence features indicate that all six species are typical New World, bipartite begomoviruses. Four of the novel species (represented by 42 DNA-A clones) clustered with Brazilian viruses in the phylogenetic tree. Interestingly, the two other new species (represented by 27 DNA-A clones) clustered with begomoviruses from Mexico and Central America. These two species could represent a distinct lineage of New World begomoviruses, found in Brazil for the first time. Analysis of the genetic structure of ToYVSV and ToCmMV populations indicates a higher degree of genetic variability for ToCmMV. Furthermore, comparison between the ToCmMV subpopulations from Coimbra and Paty do Alferes indicates greater variability in Coimbra. Considering that the presence of begomoviruses in tomatoes has been verified in Coimbra since 2001, but was verified for the first time in Paty do Alferes only in 2005, these results are consistent with a recent introduction of viral populations in Paty do Alferes, followed by a rapid populational expansion. In Coimbra, the virus and its host may have been coevolving for a longer time. Further analyses will investigate the existence of recombination events among the detected viruses, as well as their phylogeographic relationships.
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spelling Urquiza, Gloria Patricia Castillohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4778353J2Zerbini, Poliane Alfenashttp://lattes.cnpq.br/8632328159533071Mizubuti, Eduardo Seiti Gomidehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785633J8Zerbini Júnior, Francisco Murilohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5Nagata, Alice Kazuko Inouehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784060P3Fontes, Elizabeth Pacheco Batistahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781848H22015-03-26T12:41:51Z2008-08-122015-03-26T12:41:51Z2008-03-19URQUIZA, Gloria Patricia Castillo. Diversity and genetic structure of begomovirus populations in tomatoes in two growing regions of Southeastern Brazil. 2008. 117 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2008.http://locus.ufv.br/handle/123456789/1021The incidence of begomoviruses has sharply increased in Brazil since the mid 1990 s, after the introduction of the B biotype of the whitefly Bemisia tabaci. It is believed that the insect vector transferred indigenous viruses infecting wild and weed hosts to tomato. After a rapid evolutionary process, novel species adapted to the new host became prevalent in the field. To date, five begomovirus species infecting tomatoes have been characterized, and six tentative species have been partially characterized. The objectives of this work were to determine the diversity and the genetic structure of begomovirus populations infecting tomatoes and associated weeds in two major tomato growing regions of Southeastern Brazil, the cities of Paty do Alferes (Rio de Janeiro state) and Coimbra (Minas Gerais state). The genetic structure of populations refers to the degree of genetic variability and its distribution within and between subpopulations, reflecting the evolutionary history and the potential of the population to evolve. A total of 119 tomato samples were collected in Paty do Alferes in May 2005. In Coimbra, 17 tomato samples and 43 weed samples were collected in July 2007. Total DNA was extracted from the samples and full-length begomovirus genomes were cloned by rolling circle amplification. Clones were completely sequenced and the sequences were used for comparisons with previously described begomoviruses, for phylogenetic analysis and for the determination of the genetic structure of viral populations. Sequence comparisons indicated the presence of two novel begomovirus species in tomato plants collected in Paty do Alferes, three novel species in weed samples collected in Coimbra, and one novel species in tomato samples collected at both locations. Sequence features indicate that all six species are typical New World, bipartite begomoviruses. Four of the novel species (represented by 42 DNA-A clones) clustered with Brazilian viruses in the phylogenetic tree. Interestingly, the two other new species (represented by 27 DNA-A clones) clustered with begomoviruses from Mexico and Central America. These two species could represent a distinct lineage of New World begomoviruses, found in Brazil for the first time. Analysis of the genetic structure of ToYVSV and ToCmMV populations indicates a higher degree of genetic variability for ToCmMV. Furthermore, comparison between the ToCmMV subpopulations from Coimbra and Paty do Alferes indicates greater variability in Coimbra. Considering that the presence of begomoviruses in tomatoes has been verified in Coimbra since 2001, but was verified for the first time in Paty do Alferes only in 2005, these results are consistent with a recent introduction of viral populations in Paty do Alferes, followed by a rapid populational expansion. In Coimbra, the virus and its host may have been coevolving for a longer time. Further analyses will investigate the existence of recombination events among the detected viruses, as well as their phylogeographic relationships.A incidência de begomovírus em tomateiros no Brasil vem aumentando gradativamente desde meados da década de 1990, após a introdução do biótipo B da mosca-branca Bemisia tabaci. O inseto vetor teria transferido vírus nativos que infectam plantas silvestres ou daninhas para o tomateiro. Após um rápido processo evolutivo, novas espécies mais adaptadas ao novo hospedeiro passaram a predominar no campo. Atualmente, cinco espécies de begomovírus que infectam o tomateiro encontram-se caracterizadas, e seis espécies tentativas foram parcialmente caracterizadas. Este trabalho teve como objetivos determinar a diversidade e a estrutura genética de populações de begomovírus em tomateiro e em plantas daninhas em duas regiões produtoras da região Sudeste do Brasil, os municípios de Paty do Alferes (RJ) e Coimbra (MG). A estrutura genética de populações refere-se à quantidade de variabilidade genética e a sua distribuição dentro e entre subpopulações, refletindo a história evolutiva da população. Um total de 115 amostras de tomateiro foram coletadas em Paty do Alferes em maio de 2005. Em Coimbra, em julho de 2007, foram coletadas 17 amostras de tomateiro e 43 amostras de plantas daninhas. A partir de DNA extraído das amostras, os genomas de begomovírus foram clonados e completamente sequenciados. As seqüências foram comparadas com as de begomovírus previamente descritos, e foram realizadas análises filogenéticas e de determinação da estrutura genética das populações virais. Duas novas espécies virais em tomateiros foram detectadas em Paty do Alferes, três novas espécies nas plantas daninhas Blainvillea rhomboidea, Sida micrantha e S. rhombifolia, foram detectadas próximas a campos de tomateiro em Coimbra, e uma nova espécie presente em plantas de tomateiro nas duas localidades. As seis espécies virais possuem todas as características de begomovírus bissegmentados das Américas. Quatro das novas espécies (num total de 42 clones correspondentes a DNA-A) se agruparam com as demais espécies brasileiras na árvore filogenética. Entretanto, duas das novas espécies (num total de 27 clones correspondentes a DNA-A) foram agrupadas com vírus identificados no México e na América Central. Essas espécies podem pertencer a uma linhagem distinta de begomovírus, detectada no Brasil pela primeira vez. A análise da estrutura genética das populações de ToYVSV e ToCmMV indicou maior variabilidade genética em ToCmMV. Além disso, a comparação das subpopulações de Coimbra e Paty do Alferes do ToCmMV indica maior variabilidade em Coimbra. Considerando-se que a presença de begomovírus na região de Coimbra vem sendo constatada desde 2001, ao passo que em Paty do Alferes ocorreu apenas em 2005, os resultados obtidos são consistentes com a introdução recente das populações virais em Paty do Alferes, seguido de expansão populacional. Em Coimbra, o vírus e seu hospedeiro provavelmente estão co-evoluindo há mais tempo. Análises posteriores investigarão a existência de eventos de recombinação entre os diferentes vírus encontrados, bem como o relacionamento filogeográfico entre as espécies.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de ViçosaDoutorado em FitopatologiaUFVBREtiologia; Epidemiologia; ControleBegomovírusTomateGenética de populaçõesVírus de plantasBegomovirusTomatoPopulation geneticsPlant virusCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIADiversidade e estrutura genética de begomovírus em duas regiões produtoras de tomate do Sudeste do BrasilDiversity and genetic structure of begomovirus populations in tomatoes in two growing regions of Southeastern Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf701069https://locus.ufv.br//bitstream/123456789/1021/1/texto%20completo.pdfa58352382d967242dc970bdd1fde8dbaMD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain220597https://locus.ufv.br//bitstream/123456789/1021/2/texto%20completo.pdf.txt46377245f8b498a723d907ec4926432cMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3567https://locus.ufv.br//bitstream/123456789/1021/3/texto%20completo.pdf.jpg3e1a13e889aaaac0434bb42143897f26MD53123456789/10212016-04-06 23:16:50.757oai:locus.ufv.br:123456789/1021Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-07T02:16:50LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Diversidade e estrutura genética de begomovírus em duas regiões produtoras de tomate do Sudeste do Brasil
dc.title.alternative.eng.fl_str_mv Diversity and genetic structure of begomovirus populations in tomatoes in two growing regions of Southeastern Brazil
title Diversidade e estrutura genética de begomovírus em duas regiões produtoras de tomate do Sudeste do Brasil
spellingShingle Diversidade e estrutura genética de begomovírus em duas regiões produtoras de tomate do Sudeste do Brasil
Urquiza, Gloria Patricia Castillo
Begomovírus
Tomate
Genética de populações
Vírus de plantas
Begomovirus
Tomato
Population genetics
Plant virus
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
title_short Diversidade e estrutura genética de begomovírus em duas regiões produtoras de tomate do Sudeste do Brasil
title_full Diversidade e estrutura genética de begomovírus em duas regiões produtoras de tomate do Sudeste do Brasil
title_fullStr Diversidade e estrutura genética de begomovírus em duas regiões produtoras de tomate do Sudeste do Brasil
title_full_unstemmed Diversidade e estrutura genética de begomovírus em duas regiões produtoras de tomate do Sudeste do Brasil
title_sort Diversidade e estrutura genética de begomovírus em duas regiões produtoras de tomate do Sudeste do Brasil
author Urquiza, Gloria Patricia Castillo
author_facet Urquiza, Gloria Patricia Castillo
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4778353J2
dc.contributor.author.fl_str_mv Urquiza, Gloria Patricia Castillo
dc.contributor.advisor-co1.fl_str_mv Zerbini, Poliane Alfenas
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/8632328159533071
dc.contributor.advisor-co2.fl_str_mv Mizubuti, Eduardo Seiti Gomide
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785633J8
dc.contributor.advisor1.fl_str_mv Zerbini Júnior, Francisco Murilo
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783743U5
dc.contributor.referee1.fl_str_mv Nagata, Alice Kazuko Inoue
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784060P3
dc.contributor.referee2.fl_str_mv Fontes, Elizabeth Pacheco Batista
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781848H2
contributor_str_mv Zerbini, Poliane Alfenas
Mizubuti, Eduardo Seiti Gomide
Zerbini Júnior, Francisco Murilo
Nagata, Alice Kazuko Inoue
Fontes, Elizabeth Pacheco Batista
dc.subject.por.fl_str_mv Begomovírus
Tomate
Genética de populações
Vírus de plantas
topic Begomovírus
Tomate
Genética de populações
Vírus de plantas
Begomovirus
Tomato
Population genetics
Plant virus
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
dc.subject.eng.fl_str_mv Begomovirus
Tomato
Population genetics
Plant virus
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
description The incidence of begomoviruses has sharply increased in Brazil since the mid 1990 s, after the introduction of the B biotype of the whitefly Bemisia tabaci. It is believed that the insect vector transferred indigenous viruses infecting wild and weed hosts to tomato. After a rapid evolutionary process, novel species adapted to the new host became prevalent in the field. To date, five begomovirus species infecting tomatoes have been characterized, and six tentative species have been partially characterized. The objectives of this work were to determine the diversity and the genetic structure of begomovirus populations infecting tomatoes and associated weeds in two major tomato growing regions of Southeastern Brazil, the cities of Paty do Alferes (Rio de Janeiro state) and Coimbra (Minas Gerais state). The genetic structure of populations refers to the degree of genetic variability and its distribution within and between subpopulations, reflecting the evolutionary history and the potential of the population to evolve. A total of 119 tomato samples were collected in Paty do Alferes in May 2005. In Coimbra, 17 tomato samples and 43 weed samples were collected in July 2007. Total DNA was extracted from the samples and full-length begomovirus genomes were cloned by rolling circle amplification. Clones were completely sequenced and the sequences were used for comparisons with previously described begomoviruses, for phylogenetic analysis and for the determination of the genetic structure of viral populations. Sequence comparisons indicated the presence of two novel begomovirus species in tomato plants collected in Paty do Alferes, three novel species in weed samples collected in Coimbra, and one novel species in tomato samples collected at both locations. Sequence features indicate that all six species are typical New World, bipartite begomoviruses. Four of the novel species (represented by 42 DNA-A clones) clustered with Brazilian viruses in the phylogenetic tree. Interestingly, the two other new species (represented by 27 DNA-A clones) clustered with begomoviruses from Mexico and Central America. These two species could represent a distinct lineage of New World begomoviruses, found in Brazil for the first time. Analysis of the genetic structure of ToYVSV and ToCmMV populations indicates a higher degree of genetic variability for ToCmMV. Furthermore, comparison between the ToCmMV subpopulations from Coimbra and Paty do Alferes indicates greater variability in Coimbra. Considering that the presence of begomoviruses in tomatoes has been verified in Coimbra since 2001, but was verified for the first time in Paty do Alferes only in 2005, these results are consistent with a recent introduction of viral populations in Paty do Alferes, followed by a rapid populational expansion. In Coimbra, the virus and its host may have been coevolving for a longer time. Further analyses will investigate the existence of recombination events among the detected viruses, as well as their phylogeographic relationships.
publishDate 2008
dc.date.available.fl_str_mv 2008-08-12
2015-03-26T12:41:51Z
dc.date.issued.fl_str_mv 2008-03-19
dc.date.accessioned.fl_str_mv 2015-03-26T12:41:51Z
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dc.identifier.citation.fl_str_mv URQUIZA, Gloria Patricia Castillo. Diversity and genetic structure of begomovirus populations in tomatoes in two growing regions of Southeastern Brazil. 2008. 117 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2008.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/1021
identifier_str_mv URQUIZA, Gloria Patricia Castillo. Diversity and genetic structure of begomovirus populations in tomatoes in two growing regions of Southeastern Brazil. 2008. 117 f. Tese (Doutorado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2008.
url http://locus.ufv.br/handle/123456789/1021
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dc.publisher.program.fl_str_mv Doutorado em Fitopatologia
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dc.publisher.department.fl_str_mv Etiologia; Epidemiologia; Controle
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