Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1017/S1431927617012363 http://www.locus.ufv.br/handle/123456789/18731 |
Resumo: | By using an experimental model of dexamethasone-induced osteoporosis we investigated the effects of different therapeutic schemes combining sodium alendronate (SA) and simvastatin on bone mineral and protein composition, microstructural and mechanical remodeling. Wistar rats were randomized into eight groups: G1: non-osteoporotic; G2: osteoporotic; G3, G4, and G5: osteoporotic + SA (0.2, 0.4, and 0.8 mg/kg, respectively); G6, G7, and G8: osteoporotic + SA (0.2, 0.4, and 0.8 mg/kg, respectively) + simvastatin (0.4, 0.6, and 1 mg/kg, respectively). Osteoporosis was induced by dexamethasone (7 mg/kg, i.m.) once a week for 5 weeks. All treatments were administered for 8 weeks. Dexamethasone increased serum levels of alkaline phosphatase, calcium, phosphorus, and urea, especially in non-treated animals, which showed severe osteoporosis. Dexamethasone also induced bone microstructural fragility and reduced mechanical resistance, which were associated with a marked depletion in mineral mass, collagenous and non-collagenous protein levels in cortical and cancellous bone. Although SA has attenuated osteoporosis severity, the effectiveness of drug therapy was enhanced combining alendronate and simvastatin. The restoration in serum parameters, organic and inorganic bone mass, and mechanical behavior showed a dose-dependent effect that was potentially related to the complementary mechanisms by which each drug acts to induce bone anabolism, accelerating tissue repair. |
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Sequetto, Priscila L.Gonçalves, Reggiani V.Pinto, Aloísio S.Oliveira, Maria G. A.Maldonado, Izabel R. S. C.Oliveira, Tânia T.Novaes, Rômulo D.2018-04-16T17:34:36Z2018-04-16T17:34:36Z2017-06-1914358115https://doi.org/10.1017/S1431927617012363http://www.locus.ufv.br/handle/123456789/18731By using an experimental model of dexamethasone-induced osteoporosis we investigated the effects of different therapeutic schemes combining sodium alendronate (SA) and simvastatin on bone mineral and protein composition, microstructural and mechanical remodeling. Wistar rats were randomized into eight groups: G1: non-osteoporotic; G2: osteoporotic; G3, G4, and G5: osteoporotic + SA (0.2, 0.4, and 0.8 mg/kg, respectively); G6, G7, and G8: osteoporotic + SA (0.2, 0.4, and 0.8 mg/kg, respectively) + simvastatin (0.4, 0.6, and 1 mg/kg, respectively). Osteoporosis was induced by dexamethasone (7 mg/kg, i.m.) once a week for 5 weeks. All treatments were administered for 8 weeks. Dexamethasone increased serum levels of alkaline phosphatase, calcium, phosphorus, and urea, especially in non-treated animals, which showed severe osteoporosis. Dexamethasone also induced bone microstructural fragility and reduced mechanical resistance, which were associated with a marked depletion in mineral mass, collagenous and non-collagenous protein levels in cortical and cancellous bone. Although SA has attenuated osteoporosis severity, the effectiveness of drug therapy was enhanced combining alendronate and simvastatin. The restoration in serum parameters, organic and inorganic bone mass, and mechanical behavior showed a dose-dependent effect that was potentially related to the complementary mechanisms by which each drug acts to induce bone anabolism, accelerating tissue repair.engMicroscopy and Microanalysisv. 23, Issue 5, p. 989-1001, October 2017Microscopy Society of Americainfo:eu-repo/semantics/openAccessBoneGlucocorticoidMicroanalysisPathologyScanning electron microscopyLow doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf1443462https://locus.ufv.br//bitstream/123456789/18731/1/artigo.pdf4c87564670c6adfb5728d4dc4f17281bMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/18731/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5178https://locus.ufv.br//bitstream/123456789/18731/3/artigo.pdf.jpg79aaa993ee1bdd06130cf172c944eb4cMD53123456789/187312018-04-16 23:00:38.801oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-04-17T02:00:38LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis |
title |
Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis |
spellingShingle |
Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis Sequetto, Priscila L. Bone Glucocorticoid Microanalysis Pathology Scanning electron microscopy |
title_short |
Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis |
title_full |
Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis |
title_fullStr |
Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis |
title_full_unstemmed |
Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis |
title_sort |
Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis |
author |
Sequetto, Priscila L. |
author_facet |
Sequetto, Priscila L. Gonçalves, Reggiani V. Pinto, Aloísio S. Oliveira, Maria G. A. Maldonado, Izabel R. S. C. Oliveira, Tânia T. Novaes, Rômulo D. |
author_role |
author |
author2 |
Gonçalves, Reggiani V. Pinto, Aloísio S. Oliveira, Maria G. A. Maldonado, Izabel R. S. C. Oliveira, Tânia T. Novaes, Rômulo D. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Sequetto, Priscila L. Gonçalves, Reggiani V. Pinto, Aloísio S. Oliveira, Maria G. A. Maldonado, Izabel R. S. C. Oliveira, Tânia T. Novaes, Rômulo D. |
dc.subject.pt-BR.fl_str_mv |
Bone Glucocorticoid Microanalysis Pathology Scanning electron microscopy |
topic |
Bone Glucocorticoid Microanalysis Pathology Scanning electron microscopy |
description |
By using an experimental model of dexamethasone-induced osteoporosis we investigated the effects of different therapeutic schemes combining sodium alendronate (SA) and simvastatin on bone mineral and protein composition, microstructural and mechanical remodeling. Wistar rats were randomized into eight groups: G1: non-osteoporotic; G2: osteoporotic; G3, G4, and G5: osteoporotic + SA (0.2, 0.4, and 0.8 mg/kg, respectively); G6, G7, and G8: osteoporotic + SA (0.2, 0.4, and 0.8 mg/kg, respectively) + simvastatin (0.4, 0.6, and 1 mg/kg, respectively). Osteoporosis was induced by dexamethasone (7 mg/kg, i.m.) once a week for 5 weeks. All treatments were administered for 8 weeks. Dexamethasone increased serum levels of alkaline phosphatase, calcium, phosphorus, and urea, especially in non-treated animals, which showed severe osteoporosis. Dexamethasone also induced bone microstructural fragility and reduced mechanical resistance, which were associated with a marked depletion in mineral mass, collagenous and non-collagenous protein levels in cortical and cancellous bone. Although SA has attenuated osteoporosis severity, the effectiveness of drug therapy was enhanced combining alendronate and simvastatin. The restoration in serum parameters, organic and inorganic bone mass, and mechanical behavior showed a dose-dependent effect that was potentially related to the complementary mechanisms by which each drug acts to induce bone anabolism, accelerating tissue repair. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-06-19 |
dc.date.accessioned.fl_str_mv |
2018-04-16T17:34:36Z |
dc.date.available.fl_str_mv |
2018-04-16T17:34:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1017/S1431927617012363 http://www.locus.ufv.br/handle/123456789/18731 |
dc.identifier.issn.none.fl_str_mv |
14358115 |
identifier_str_mv |
14358115 |
url |
https://doi.org/10.1017/S1431927617012363 http://www.locus.ufv.br/handle/123456789/18731 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 23, Issue 5, p. 989-1001, October 2017 |
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Microscopy Society of America info:eu-repo/semantics/openAccess |
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Microscopy Society of America |
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openAccess |
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Microscopy and Microanalysis |
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Microscopy and Microanalysis |
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