Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis

Detalhes bibliográficos
Autor(a) principal: Sequetto, Priscila L.
Data de Publicação: 2017
Outros Autores: Gonçalves, Reggiani V., Pinto, Aloísio S., Oliveira, Maria G. A., Maldonado, Izabel R. S. C., Oliveira, Tânia T., Novaes, Rômulo D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1017/S1431927617012363
http://www.locus.ufv.br/handle/123456789/18731
Resumo: By using an experimental model of dexamethasone-induced osteoporosis we investigated the effects of different therapeutic schemes combining sodium alendronate (SA) and simvastatin on bone mineral and protein composition, microstructural and mechanical remodeling. Wistar rats were randomized into eight groups: G1: non-osteoporotic; G2: osteoporotic; G3, G4, and G5: osteoporotic + SA (0.2, 0.4, and 0.8 mg/kg, respectively); G6, G7, and G8: osteoporotic + SA (0.2, 0.4, and 0.8 mg/kg, respectively) + simvastatin (0.4, 0.6, and 1 mg/kg, respectively). Osteoporosis was induced by dexamethasone (7 mg/kg, i.m.) once a week for 5 weeks. All treatments were administered for 8 weeks. Dexamethasone increased serum levels of alkaline phosphatase, calcium, phosphorus, and urea, especially in non-treated animals, which showed severe osteoporosis. Dexamethasone also induced bone microstructural fragility and reduced mechanical resistance, which were associated with a marked depletion in mineral mass, collagenous and non-collagenous protein levels in cortical and cancellous bone. Although SA has attenuated osteoporosis severity, the effectiveness of drug therapy was enhanced combining alendronate and simvastatin. The restoration in serum parameters, organic and inorganic bone mass, and mechanical behavior showed a dose-dependent effect that was potentially related to the complementary mechanisms by which each drug acts to induce bone anabolism, accelerating tissue repair.
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spelling Sequetto, Priscila L.Gonçalves, Reggiani V.Pinto, Aloísio S.Oliveira, Maria G. A.Maldonado, Izabel R. S. C.Oliveira, Tânia T.Novaes, Rômulo D.2018-04-16T17:34:36Z2018-04-16T17:34:36Z2017-06-1914358115https://doi.org/10.1017/S1431927617012363http://www.locus.ufv.br/handle/123456789/18731By using an experimental model of dexamethasone-induced osteoporosis we investigated the effects of different therapeutic schemes combining sodium alendronate (SA) and simvastatin on bone mineral and protein composition, microstructural and mechanical remodeling. Wistar rats were randomized into eight groups: G1: non-osteoporotic; G2: osteoporotic; G3, G4, and G5: osteoporotic + SA (0.2, 0.4, and 0.8 mg/kg, respectively); G6, G7, and G8: osteoporotic + SA (0.2, 0.4, and 0.8 mg/kg, respectively) + simvastatin (0.4, 0.6, and 1 mg/kg, respectively). Osteoporosis was induced by dexamethasone (7 mg/kg, i.m.) once a week for 5 weeks. All treatments were administered for 8 weeks. Dexamethasone increased serum levels of alkaline phosphatase, calcium, phosphorus, and urea, especially in non-treated animals, which showed severe osteoporosis. Dexamethasone also induced bone microstructural fragility and reduced mechanical resistance, which were associated with a marked depletion in mineral mass, collagenous and non-collagenous protein levels in cortical and cancellous bone. Although SA has attenuated osteoporosis severity, the effectiveness of drug therapy was enhanced combining alendronate and simvastatin. The restoration in serum parameters, organic and inorganic bone mass, and mechanical behavior showed a dose-dependent effect that was potentially related to the complementary mechanisms by which each drug acts to induce bone anabolism, accelerating tissue repair.engMicroscopy and Microanalysisv. 23, Issue 5, p. 989-1001, October 2017Microscopy Society of Americainfo:eu-repo/semantics/openAccessBoneGlucocorticoidMicroanalysisPathologyScanning electron microscopyLow doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf1443462https://locus.ufv.br//bitstream/123456789/18731/1/artigo.pdf4c87564670c6adfb5728d4dc4f17281bMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/18731/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5178https://locus.ufv.br//bitstream/123456789/18731/3/artigo.pdf.jpg79aaa993ee1bdd06130cf172c944eb4cMD53123456789/187312018-04-16 23:00:38.801oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-04-17T02:00:38LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis
title Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis
spellingShingle Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis
Sequetto, Priscila L.
Bone
Glucocorticoid
Microanalysis
Pathology
Scanning electron microscopy
title_short Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis
title_full Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis
title_fullStr Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis
title_full_unstemmed Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis
title_sort Low doses of simvastatin potentiate the effect of sodium alendronate in inhibiting bone resorption and rtestore microstructural and mechanical bone properties in Glucocorticoid-Induced Osteoporosis
author Sequetto, Priscila L.
author_facet Sequetto, Priscila L.
Gonçalves, Reggiani V.
Pinto, Aloísio S.
Oliveira, Maria G. A.
Maldonado, Izabel R. S. C.
Oliveira, Tânia T.
Novaes, Rômulo D.
author_role author
author2 Gonçalves, Reggiani V.
Pinto, Aloísio S.
Oliveira, Maria G. A.
Maldonado, Izabel R. S. C.
Oliveira, Tânia T.
Novaes, Rômulo D.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sequetto, Priscila L.
Gonçalves, Reggiani V.
Pinto, Aloísio S.
Oliveira, Maria G. A.
Maldonado, Izabel R. S. C.
Oliveira, Tânia T.
Novaes, Rômulo D.
dc.subject.pt-BR.fl_str_mv Bone
Glucocorticoid
Microanalysis
Pathology
Scanning electron microscopy
topic Bone
Glucocorticoid
Microanalysis
Pathology
Scanning electron microscopy
description By using an experimental model of dexamethasone-induced osteoporosis we investigated the effects of different therapeutic schemes combining sodium alendronate (SA) and simvastatin on bone mineral and protein composition, microstructural and mechanical remodeling. Wistar rats were randomized into eight groups: G1: non-osteoporotic; G2: osteoporotic; G3, G4, and G5: osteoporotic + SA (0.2, 0.4, and 0.8 mg/kg, respectively); G6, G7, and G8: osteoporotic + SA (0.2, 0.4, and 0.8 mg/kg, respectively) + simvastatin (0.4, 0.6, and 1 mg/kg, respectively). Osteoporosis was induced by dexamethasone (7 mg/kg, i.m.) once a week for 5 weeks. All treatments were administered for 8 weeks. Dexamethasone increased serum levels of alkaline phosphatase, calcium, phosphorus, and urea, especially in non-treated animals, which showed severe osteoporosis. Dexamethasone also induced bone microstructural fragility and reduced mechanical resistance, which were associated with a marked depletion in mineral mass, collagenous and non-collagenous protein levels in cortical and cancellous bone. Although SA has attenuated osteoporosis severity, the effectiveness of drug therapy was enhanced combining alendronate and simvastatin. The restoration in serum parameters, organic and inorganic bone mass, and mechanical behavior showed a dose-dependent effect that was potentially related to the complementary mechanisms by which each drug acts to induce bone anabolism, accelerating tissue repair.
publishDate 2017
dc.date.issued.fl_str_mv 2017-06-19
dc.date.accessioned.fl_str_mv 2018-04-16T17:34:36Z
dc.date.available.fl_str_mv 2018-04-16T17:34:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv https://doi.org/10.1017/S1431927617012363
http://www.locus.ufv.br/handle/123456789/18731
dc.identifier.issn.none.fl_str_mv 14358115
identifier_str_mv 14358115
url https://doi.org/10.1017/S1431927617012363
http://www.locus.ufv.br/handle/123456789/18731
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv v. 23, Issue 5, p. 989-1001, October 2017
dc.rights.driver.fl_str_mv Microscopy Society of America
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