Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection

Detalhes bibliográficos
Autor(a) principal: Mendes, Tiago Antônio de Oliveira
Data de Publicação: 2017
Outros Autores: Passos, Lívia Silva Araújo, Gazzinelli-Guimarães, Pedro Henrique, Guimarães, Ana Clara Gazzinelli, Lemos, Denise da Silveira, Ricci, Natasha Delaqua, Gonçalves, Ricardo, Bartholomeu, Daniella Castanheira, Fujiwara, Ricardo Toshio, Bueno, Lilian Lacerda
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1186/s12879-017-2366-0
http://www.locus.ufv.br/handle/123456789/12468
Resumo: While the macrophage polarization is well characterized in helminth infections, the natural heterogeneity of monocytes with multiple cell phenotypes might influence the outcome of neglected diseases, such hookworm infection. Here, we report the profile of monocytes in human hookworm infections as a model to study the regulatory subpopulation of monocytes in helminth infections. Blood samples were collected from 19 Necator americanus-infected individuals and 13 healthy individuals. Peripheral blood mononuclear cells (PBMCs) were isolated, and immunophenotyping was conducted by flow cytometry. The expressions of genes encoding human nitric oxide synthase (iNOS), interleukin 4 (IL-4), arginase-1 (Arg-1) and glyceraldehyde 3-phosphate dehydrogenase were quantified by qPCR. Plasma levels of IL-4 were determined by sandwich ELISA. Unpaired t-tests or Mann-Whitney tests were used depending on the data distribution.Hookworm infected individuals (HWI) showed a significant increase in the number of monocytes/mm3 (555.2 ± 191.0) compared to that of the non-infected (NI) individuals (120.4 ± 44.7) (p < 0.0001). While the frequencies of CD14+IL-10+ and CD14+IL-12+ cells were significantly reduced in the HWI compared to NI group (p = 0.0289 and p < 0.0001, respectively), the ratio between IL-10/IL-12 producing monocytes was significantly elevated in HWI (p = 0.0004), indicating the potential regulatory activity of these cells. Measurement of IL-4 levels and gene expression of IL-4 and Arg-1 (highly expressed in alternatively activated macrophages) revealed no significant differences between the NI and HWI groups. Interestingly, individuals from the HWI group had higher expression of the iNOS gene (associated with a regulatory profile) (20.27 ± 2.97) compared to the NI group (11.28 ± 1.18, p = 0.0409). Finally, individuals from the HWI group had a significantly higher frequency of CD206+CD23+IL-10+ (7.57 ± 1.96) cells compared to individuals from the NI group (0.35 ± 0.09) (p < 0.001), suggesting that activated monocytes are a potential source of regulatory cytokines during hookworm infection.Natural hookworm infection induces a high frequency of circulating monocytes that present a regulatory profile and promote the downmodulation of the proinflammatory response, which may contribute to prolonged survival of the parasite in the host.
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spelling Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infectionRegulatory monocytesHelminth infectionsModulationInfectionHuman hookwormWhile the macrophage polarization is well characterized in helminth infections, the natural heterogeneity of monocytes with multiple cell phenotypes might influence the outcome of neglected diseases, such hookworm infection. Here, we report the profile of monocytes in human hookworm infections as a model to study the regulatory subpopulation of monocytes in helminth infections. Blood samples were collected from 19 Necator americanus-infected individuals and 13 healthy individuals. Peripheral blood mononuclear cells (PBMCs) were isolated, and immunophenotyping was conducted by flow cytometry. The expressions of genes encoding human nitric oxide synthase (iNOS), interleukin 4 (IL-4), arginase-1 (Arg-1) and glyceraldehyde 3-phosphate dehydrogenase were quantified by qPCR. Plasma levels of IL-4 were determined by sandwich ELISA. Unpaired t-tests or Mann-Whitney tests were used depending on the data distribution.Hookworm infected individuals (HWI) showed a significant increase in the number of monocytes/mm3 (555.2 ± 191.0) compared to that of the non-infected (NI) individuals (120.4 ± 44.7) (p < 0.0001). While the frequencies of CD14+IL-10+ and CD14+IL-12+ cells were significantly reduced in the HWI compared to NI group (p = 0.0289 and p < 0.0001, respectively), the ratio between IL-10/IL-12 producing monocytes was significantly elevated in HWI (p = 0.0004), indicating the potential regulatory activity of these cells. Measurement of IL-4 levels and gene expression of IL-4 and Arg-1 (highly expressed in alternatively activated macrophages) revealed no significant differences between the NI and HWI groups. Interestingly, individuals from the HWI group had higher expression of the iNOS gene (associated with a regulatory profile) (20.27 ± 2.97) compared to the NI group (11.28 ± 1.18, p = 0.0409). Finally, individuals from the HWI group had a significantly higher frequency of CD206+CD23+IL-10+ (7.57 ± 1.96) cells compared to individuals from the NI group (0.35 ± 0.09) (p < 0.001), suggesting that activated monocytes are a potential source of regulatory cytokines during hookworm infection.Natural hookworm infection induces a high frequency of circulating monocytes that present a regulatory profile and promote the downmodulation of the proinflammatory response, which may contribute to prolonged survival of the parasite in the host.BMC Infectious Diseases2017-10-26T17:34:18Z2017-10-26T17:34:18Z2017-04-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf1471-2334https://doi.org/10.1186/s12879-017-2366-0http://www.locus.ufv.br/handle/123456789/12468eng17:253, April 2017Mendes, Tiago Antônio de OliveiraPassos, Lívia Silva AraújoGazzinelli-Guimarães, Pedro HenriqueGuimarães, Ana Clara GazzinelliLemos, Denise da SilveiraRicci, Natasha DelaquaGonçalves, RicardoBartholomeu, Daniella CastanheiraFujiwara, Ricardo ToshioBueno, Lilian Lacerdainfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:19:04Zoai:locus.ufv.br:123456789/12468Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:19:04LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection
title Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection
spellingShingle Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection
Mendes, Tiago Antônio de Oliveira
Regulatory monocytes
Helminth infections
Modulation
Infection
Human hookworm
title_short Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection
title_full Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection
title_fullStr Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection
title_full_unstemmed Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection
title_sort Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection
author Mendes, Tiago Antônio de Oliveira
author_facet Mendes, Tiago Antônio de Oliveira
Passos, Lívia Silva Araújo
Gazzinelli-Guimarães, Pedro Henrique
Guimarães, Ana Clara Gazzinelli
Lemos, Denise da Silveira
Ricci, Natasha Delaqua
Gonçalves, Ricardo
Bartholomeu, Daniella Castanheira
Fujiwara, Ricardo Toshio
Bueno, Lilian Lacerda
author_role author
author2 Passos, Lívia Silva Araújo
Gazzinelli-Guimarães, Pedro Henrique
Guimarães, Ana Clara Gazzinelli
Lemos, Denise da Silveira
Ricci, Natasha Delaqua
Gonçalves, Ricardo
Bartholomeu, Daniella Castanheira
Fujiwara, Ricardo Toshio
Bueno, Lilian Lacerda
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mendes, Tiago Antônio de Oliveira
Passos, Lívia Silva Araújo
Gazzinelli-Guimarães, Pedro Henrique
Guimarães, Ana Clara Gazzinelli
Lemos, Denise da Silveira
Ricci, Natasha Delaqua
Gonçalves, Ricardo
Bartholomeu, Daniella Castanheira
Fujiwara, Ricardo Toshio
Bueno, Lilian Lacerda
dc.subject.por.fl_str_mv Regulatory monocytes
Helminth infections
Modulation
Infection
Human hookworm
topic Regulatory monocytes
Helminth infections
Modulation
Infection
Human hookworm
description While the macrophage polarization is well characterized in helminth infections, the natural heterogeneity of monocytes with multiple cell phenotypes might influence the outcome of neglected diseases, such hookworm infection. Here, we report the profile of monocytes in human hookworm infections as a model to study the regulatory subpopulation of monocytes in helminth infections. Blood samples were collected from 19 Necator americanus-infected individuals and 13 healthy individuals. Peripheral blood mononuclear cells (PBMCs) were isolated, and immunophenotyping was conducted by flow cytometry. The expressions of genes encoding human nitric oxide synthase (iNOS), interleukin 4 (IL-4), arginase-1 (Arg-1) and glyceraldehyde 3-phosphate dehydrogenase were quantified by qPCR. Plasma levels of IL-4 were determined by sandwich ELISA. Unpaired t-tests or Mann-Whitney tests were used depending on the data distribution.Hookworm infected individuals (HWI) showed a significant increase in the number of monocytes/mm3 (555.2 ± 191.0) compared to that of the non-infected (NI) individuals (120.4 ± 44.7) (p < 0.0001). While the frequencies of CD14+IL-10+ and CD14+IL-12+ cells were significantly reduced in the HWI compared to NI group (p = 0.0289 and p < 0.0001, respectively), the ratio between IL-10/IL-12 producing monocytes was significantly elevated in HWI (p = 0.0004), indicating the potential regulatory activity of these cells. Measurement of IL-4 levels and gene expression of IL-4 and Arg-1 (highly expressed in alternatively activated macrophages) revealed no significant differences between the NI and HWI groups. Interestingly, individuals from the HWI group had higher expression of the iNOS gene (associated with a regulatory profile) (20.27 ± 2.97) compared to the NI group (11.28 ± 1.18, p = 0.0409). Finally, individuals from the HWI group had a significantly higher frequency of CD206+CD23+IL-10+ (7.57 ± 1.96) cells compared to individuals from the NI group (0.35 ± 0.09) (p < 0.001), suggesting that activated monocytes are a potential source of regulatory cytokines during hookworm infection.Natural hookworm infection induces a high frequency of circulating monocytes that present a regulatory profile and promote the downmodulation of the proinflammatory response, which may contribute to prolonged survival of the parasite in the host.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-26T17:34:18Z
2017-10-26T17:34:18Z
2017-04-08
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 1471-2334
https://doi.org/10.1186/s12879-017-2366-0
http://www.locus.ufv.br/handle/123456789/12468
identifier_str_mv 1471-2334
url https://doi.org/10.1186/s12879-017-2366-0
http://www.locus.ufv.br/handle/123456789/12468
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 17:253, April 2017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv BMC Infectious Diseases
publisher.none.fl_str_mv BMC Infectious Diseases
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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