Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1186/s12879-017-2366-0 http://www.locus.ufv.br/handle/123456789/12468 |
Resumo: | While the macrophage polarization is well characterized in helminth infections, the natural heterogeneity of monocytes with multiple cell phenotypes might influence the outcome of neglected diseases, such hookworm infection. Here, we report the profile of monocytes in human hookworm infections as a model to study the regulatory subpopulation of monocytes in helminth infections. Blood samples were collected from 19 Necator americanus-infected individuals and 13 healthy individuals. Peripheral blood mononuclear cells (PBMCs) were isolated, and immunophenotyping was conducted by flow cytometry. The expressions of genes encoding human nitric oxide synthase (iNOS), interleukin 4 (IL-4), arginase-1 (Arg-1) and glyceraldehyde 3-phosphate dehydrogenase were quantified by qPCR. Plasma levels of IL-4 were determined by sandwich ELISA. Unpaired t-tests or Mann-Whitney tests were used depending on the data distribution.Hookworm infected individuals (HWI) showed a significant increase in the number of monocytes/mm3 (555.2 ± 191.0) compared to that of the non-infected (NI) individuals (120.4 ± 44.7) (p < 0.0001). While the frequencies of CD14+IL-10+ and CD14+IL-12+ cells were significantly reduced in the HWI compared to NI group (p = 0.0289 and p < 0.0001, respectively), the ratio between IL-10/IL-12 producing monocytes was significantly elevated in HWI (p = 0.0004), indicating the potential regulatory activity of these cells. Measurement of IL-4 levels and gene expression of IL-4 and Arg-1 (highly expressed in alternatively activated macrophages) revealed no significant differences between the NI and HWI groups. Interestingly, individuals from the HWI group had higher expression of the iNOS gene (associated with a regulatory profile) (20.27 ± 2.97) compared to the NI group (11.28 ± 1.18, p = 0.0409). Finally, individuals from the HWI group had a significantly higher frequency of CD206+CD23+IL-10+ (7.57 ± 1.96) cells compared to individuals from the NI group (0.35 ± 0.09) (p < 0.001), suggesting that activated monocytes are a potential source of regulatory cytokines during hookworm infection.Natural hookworm infection induces a high frequency of circulating monocytes that present a regulatory profile and promote the downmodulation of the proinflammatory response, which may contribute to prolonged survival of the parasite in the host. |
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Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infectionRegulatory monocytesHelminth infectionsModulationInfectionHuman hookwormWhile the macrophage polarization is well characterized in helminth infections, the natural heterogeneity of monocytes with multiple cell phenotypes might influence the outcome of neglected diseases, such hookworm infection. Here, we report the profile of monocytes in human hookworm infections as a model to study the regulatory subpopulation of monocytes in helminth infections. Blood samples were collected from 19 Necator americanus-infected individuals and 13 healthy individuals. Peripheral blood mononuclear cells (PBMCs) were isolated, and immunophenotyping was conducted by flow cytometry. The expressions of genes encoding human nitric oxide synthase (iNOS), interleukin 4 (IL-4), arginase-1 (Arg-1) and glyceraldehyde 3-phosphate dehydrogenase were quantified by qPCR. Plasma levels of IL-4 were determined by sandwich ELISA. Unpaired t-tests or Mann-Whitney tests were used depending on the data distribution.Hookworm infected individuals (HWI) showed a significant increase in the number of monocytes/mm3 (555.2 ± 191.0) compared to that of the non-infected (NI) individuals (120.4 ± 44.7) (p < 0.0001). While the frequencies of CD14+IL-10+ and CD14+IL-12+ cells were significantly reduced in the HWI compared to NI group (p = 0.0289 and p < 0.0001, respectively), the ratio between IL-10/IL-12 producing monocytes was significantly elevated in HWI (p = 0.0004), indicating the potential regulatory activity of these cells. Measurement of IL-4 levels and gene expression of IL-4 and Arg-1 (highly expressed in alternatively activated macrophages) revealed no significant differences between the NI and HWI groups. Interestingly, individuals from the HWI group had higher expression of the iNOS gene (associated with a regulatory profile) (20.27 ± 2.97) compared to the NI group (11.28 ± 1.18, p = 0.0409). Finally, individuals from the HWI group had a significantly higher frequency of CD206+CD23+IL-10+ (7.57 ± 1.96) cells compared to individuals from the NI group (0.35 ± 0.09) (p < 0.001), suggesting that activated monocytes are a potential source of regulatory cytokines during hookworm infection.Natural hookworm infection induces a high frequency of circulating monocytes that present a regulatory profile and promote the downmodulation of the proinflammatory response, which may contribute to prolonged survival of the parasite in the host.BMC Infectious Diseases2017-10-26T17:34:18Z2017-10-26T17:34:18Z2017-04-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf1471-2334https://doi.org/10.1186/s12879-017-2366-0http://www.locus.ufv.br/handle/123456789/12468eng17:253, April 2017Mendes, Tiago Antônio de OliveiraPassos, Lívia Silva AraújoGazzinelli-Guimarães, Pedro HenriqueGuimarães, Ana Clara GazzinelliLemos, Denise da SilveiraRicci, Natasha DelaquaGonçalves, RicardoBartholomeu, Daniella CastanheiraFujiwara, Ricardo ToshioBueno, Lilian Lacerdainfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:19:04Zoai:locus.ufv.br:123456789/12468Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:19:04LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection |
title |
Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection |
spellingShingle |
Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection Mendes, Tiago Antônio de Oliveira Regulatory monocytes Helminth infections Modulation Infection Human hookworm |
title_short |
Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection |
title_full |
Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection |
title_fullStr |
Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection |
title_full_unstemmed |
Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection |
title_sort |
Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection |
author |
Mendes, Tiago Antônio de Oliveira |
author_facet |
Mendes, Tiago Antônio de Oliveira Passos, Lívia Silva Araújo Gazzinelli-Guimarães, Pedro Henrique Guimarães, Ana Clara Gazzinelli Lemos, Denise da Silveira Ricci, Natasha Delaqua Gonçalves, Ricardo Bartholomeu, Daniella Castanheira Fujiwara, Ricardo Toshio Bueno, Lilian Lacerda |
author_role |
author |
author2 |
Passos, Lívia Silva Araújo Gazzinelli-Guimarães, Pedro Henrique Guimarães, Ana Clara Gazzinelli Lemos, Denise da Silveira Ricci, Natasha Delaqua Gonçalves, Ricardo Bartholomeu, Daniella Castanheira Fujiwara, Ricardo Toshio Bueno, Lilian Lacerda |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Mendes, Tiago Antônio de Oliveira Passos, Lívia Silva Araújo Gazzinelli-Guimarães, Pedro Henrique Guimarães, Ana Clara Gazzinelli Lemos, Denise da Silveira Ricci, Natasha Delaqua Gonçalves, Ricardo Bartholomeu, Daniella Castanheira Fujiwara, Ricardo Toshio Bueno, Lilian Lacerda |
dc.subject.por.fl_str_mv |
Regulatory monocytes Helminth infections Modulation Infection Human hookworm |
topic |
Regulatory monocytes Helminth infections Modulation Infection Human hookworm |
description |
While the macrophage polarization is well characterized in helminth infections, the natural heterogeneity of monocytes with multiple cell phenotypes might influence the outcome of neglected diseases, such hookworm infection. Here, we report the profile of monocytes in human hookworm infections as a model to study the regulatory subpopulation of monocytes in helminth infections. Blood samples were collected from 19 Necator americanus-infected individuals and 13 healthy individuals. Peripheral blood mononuclear cells (PBMCs) were isolated, and immunophenotyping was conducted by flow cytometry. The expressions of genes encoding human nitric oxide synthase (iNOS), interleukin 4 (IL-4), arginase-1 (Arg-1) and glyceraldehyde 3-phosphate dehydrogenase were quantified by qPCR. Plasma levels of IL-4 were determined by sandwich ELISA. Unpaired t-tests or Mann-Whitney tests were used depending on the data distribution.Hookworm infected individuals (HWI) showed a significant increase in the number of monocytes/mm3 (555.2 ± 191.0) compared to that of the non-infected (NI) individuals (120.4 ± 44.7) (p < 0.0001). While the frequencies of CD14+IL-10+ and CD14+IL-12+ cells were significantly reduced in the HWI compared to NI group (p = 0.0289 and p < 0.0001, respectively), the ratio between IL-10/IL-12 producing monocytes was significantly elevated in HWI (p = 0.0004), indicating the potential regulatory activity of these cells. Measurement of IL-4 levels and gene expression of IL-4 and Arg-1 (highly expressed in alternatively activated macrophages) revealed no significant differences between the NI and HWI groups. Interestingly, individuals from the HWI group had higher expression of the iNOS gene (associated with a regulatory profile) (20.27 ± 2.97) compared to the NI group (11.28 ± 1.18, p = 0.0409). Finally, individuals from the HWI group had a significantly higher frequency of CD206+CD23+IL-10+ (7.57 ± 1.96) cells compared to individuals from the NI group (0.35 ± 0.09) (p < 0.001), suggesting that activated monocytes are a potential source of regulatory cytokines during hookworm infection.Natural hookworm infection induces a high frequency of circulating monocytes that present a regulatory profile and promote the downmodulation of the proinflammatory response, which may contribute to prolonged survival of the parasite in the host. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-26T17:34:18Z 2017-10-26T17:34:18Z 2017-04-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
1471-2334 https://doi.org/10.1186/s12879-017-2366-0 http://www.locus.ufv.br/handle/123456789/12468 |
identifier_str_mv |
1471-2334 |
url |
https://doi.org/10.1186/s12879-017-2366-0 http://www.locus.ufv.br/handle/123456789/12468 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
17:253, April 2017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
pdf application/pdf |
dc.publisher.none.fl_str_mv |
BMC Infectious Diseases |
publisher.none.fl_str_mv |
BMC Infectious Diseases |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1822610621710991360 |