Efeito da adição de compostos fenólicos sobre o crescimento de levedura etanologênica (Saccharomyces sp.) em meio sintético e em caldo de cana-de-açúcar

• Autor(a) principal: Silva, Marcus Vinicius Bastos da
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2931
• topic: Compostos fenólicos; Fermentação alcoólica; Biodisponibilidade; Saccharomyces cerevisae; Phenolic compounds; Phenolic compounds; Alcoholic fermentation; Bioavailability; Saccharomyces cerevisiae; CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS

This study aimed to evaluate the effect of phenolic compounds addition on the etanologenic yeast growth and fermentation activity (Saccharomyces sp.) in synthetic medium (YPD) and within broths of two cane sugar varieties. Most of these compounds are soluble in organic solvents such as methanol, ethanol, ethyl acetate and others. For solubilization of the phenolic compounds added were evaluated these three solvents, and their influence on etanologenic yeast growth in the growth medium YPD (2 % glucose) at 28 ° C. Among the concentrations 0, 2, 4, 8 and 16 % and the solvents tested, ethanol concentration of 2%, affected less the growth of etanologenic yeast, thus, it was selected for the other tests. Using this selected solvent, tests were performed with ferulic acid, quercetin, resveratrol and rutin added at these concentrations: 0, 5, 25, 50 and 100 μg.mL-1 and 1,0 x 10-4, 1,5 x 10-4, 2,0 x 10-4 and 2,5 x 10-4 μmol.mL-1. These tests showed that phenolic compounds have a protective effect against inhibition by ethanol, related to the yeast growth and among these compounds, the best is rutin. Posteriorly, experiments were made with broths of cane sugar of these varieties, RB 867515 and RB 92579, with higher and lower levels of phenolic compounds, respectively. The fermentation tests with cane sugar broths from two varieties were analyzed with and without addition of rutin within three repetitions. It was confirmed the protective effect of rutin on ethanol as an inhibitor agent of oxidant activity.