Atividade da urease e crescimento de alface no solo em resposta a níquel

Detalhes bibliográficos
Autor(a) principal: Oliveira, Thomas Carlos de
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/5416
Resumo: The studies with Ni in soils and plants, in general, are more related to its toxicity effects to plants with emphasis in the hability of Ni hyperacumulator plants to tolerate high Ni concentrations. In recent years, this approach has been changed with more attention to the Ni participation in the plant mineral nutrition. It has to be noted that Ni is related to the plant N metabolism, due to its participation in the activity of the enzyme urease which mediate the transformation of urea in NH3 and CO2. The aim of this research was to evaluate the growth of lettuce (Lactuca sativa L.) in response to soil Ni fertilization; the effects of Ni in the urease activity in plants supplied with urea (CO(NH2)2) and ammonium nitrate (NH4NO3) as N sources; to compare the efficiency of Mehlich-3 and DTPA extractions in the determination of Ni availability in a Red-Yellow Latossol (Oxysol). The experiment was set as a 2 x 8 (2 N sources: CO (NH2)2 and NH4NO3; 8 Ni doses: 0, 2, 4, 8, 12, 16, 24 and 32 mg/dm3) with 5 replications, in an entirely ramdomly block design. The experimental unity was a 3 dm3 plastic vase with soil, treatments and two lettuce plants. Nitrogen (200 mg/dm3) was supplied as urea (Ur) or ammonium nitrate (AN), half in the seedling and half surface dressed, and Ni was supplied as NiCl2 at the seedling. One lettuce plant, per vase, was harvested 63 days after seedling and oven dried for the determination of the dry matter mass (DM) and analysis of the plant mineral composition. One week later, the other plant was harvested and its fresh matter used for the determination of urease activity by the method of indophenols. In the dry matter it was determined the dry matter contents of K, Ca, Mg, Mn, Cu, Zn, Ni (nitric-perchloric digestion and ICP-OES dosage) and N by the Kjeldahl method. In the soil it was determined the available Ni by Mehlich-3 and DTPA extractions and AAS dosage. The analysis of variance of the data was made and regression equations were adjusted for dry matter production, urease activity, soil nutrient and plant nutrient concentrations, as a function of the Ni doses applied to soil, for each N source, Ur and NA. Analysis of correlation was made between the concentrations and contents of Ni in the lettuce leaves and soil extracted Ni by Mehlich-3 and DTPA. Additionally, linear regression equations were adjusted for the plant Ni contents and the Mehlich-3 and DTPA soil recovered Ni, as a function of the Ni doses applied to soil. There was significant difference in the dry matter yield between theplants supplied by different N sources (5.07 g/plant mean for NA supplied plants and 4.20 g/plant mean for Ur supplied plants). For Ni doses applied to soil there was no significant difference in the lettuce dry matter yield whereas for the urease activity there was increase as Ni was added to soil. There was no difference between the N sources (Ur and AN) as related to the urease activity in the lettuce leaves. Smaller N concentration was detected in plants grown under the AN soil treatments, which was probably caused by a dilution effect. The total N in the plants was uniform in response to the increased Ni doses applied to the soil. The amounts of Ni recovered by Mehlich-3 and DTPA from the Red-Yellow Latossol increased as a function of the increase in the soil applied Ni. The higher correlation was shown between the Ni extracted from soil with Mehlich-3 and the plant Ni contents and concentrations. The bigger difference between the results from Mehlich-3 and DTPA soil extraction was shown in the treatments with AN added to soil.
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spelling Oliveira, Thomas Carlos deVenegas, Victor Hugo Alvarezhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727865T0Cecon, Paulo Robertohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788114T5Fontes, Renildes Lúcio Ferreirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781182P3Cantarutti, Reinaldo Bertolahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780430A1Rezende, Sebastião Tavares dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787599A32015-03-26T13:53:07Z2009-08-072015-03-26T13:53:07Z2009-03-31OLIVEIRA, Thomas Carlos de. Urease activity and lettuce growth in response to soil added nickel. 2009. 50 f. Dissertação (Mestrado em Fertilidade do solo e nutrição de plantas; Gênese, Morfologia e Classificação, Mineralogia, Química,) - Universidade Federal de Viçosa, Viçosa, 2009.http://locus.ufv.br/handle/123456789/5416The studies with Ni in soils and plants, in general, are more related to its toxicity effects to plants with emphasis in the hability of Ni hyperacumulator plants to tolerate high Ni concentrations. In recent years, this approach has been changed with more attention to the Ni participation in the plant mineral nutrition. It has to be noted that Ni is related to the plant N metabolism, due to its participation in the activity of the enzyme urease which mediate the transformation of urea in NH3 and CO2. The aim of this research was to evaluate the growth of lettuce (Lactuca sativa L.) in response to soil Ni fertilization; the effects of Ni in the urease activity in plants supplied with urea (CO(NH2)2) and ammonium nitrate (NH4NO3) as N sources; to compare the efficiency of Mehlich-3 and DTPA extractions in the determination of Ni availability in a Red-Yellow Latossol (Oxysol). The experiment was set as a 2 x 8 (2 N sources: CO (NH2)2 and NH4NO3; 8 Ni doses: 0, 2, 4, 8, 12, 16, 24 and 32 mg/dm3) with 5 replications, in an entirely ramdomly block design. The experimental unity was a 3 dm3 plastic vase with soil, treatments and two lettuce plants. Nitrogen (200 mg/dm3) was supplied as urea (Ur) or ammonium nitrate (AN), half in the seedling and half surface dressed, and Ni was supplied as NiCl2 at the seedling. One lettuce plant, per vase, was harvested 63 days after seedling and oven dried for the determination of the dry matter mass (DM) and analysis of the plant mineral composition. One week later, the other plant was harvested and its fresh matter used for the determination of urease activity by the method of indophenols. In the dry matter it was determined the dry matter contents of K, Ca, Mg, Mn, Cu, Zn, Ni (nitric-perchloric digestion and ICP-OES dosage) and N by the Kjeldahl method. In the soil it was determined the available Ni by Mehlich-3 and DTPA extractions and AAS dosage. The analysis of variance of the data was made and regression equations were adjusted for dry matter production, urease activity, soil nutrient and plant nutrient concentrations, as a function of the Ni doses applied to soil, for each N source, Ur and NA. Analysis of correlation was made between the concentrations and contents of Ni in the lettuce leaves and soil extracted Ni by Mehlich-3 and DTPA. Additionally, linear regression equations were adjusted for the plant Ni contents and the Mehlich-3 and DTPA soil recovered Ni, as a function of the Ni doses applied to soil. There was significant difference in the dry matter yield between theplants supplied by different N sources (5.07 g/plant mean for NA supplied plants and 4.20 g/plant mean for Ur supplied plants). For Ni doses applied to soil there was no significant difference in the lettuce dry matter yield whereas for the urease activity there was increase as Ni was added to soil. There was no difference between the N sources (Ur and AN) as related to the urease activity in the lettuce leaves. Smaller N concentration was detected in plants grown under the AN soil treatments, which was probably caused by a dilution effect. The total N in the plants was uniform in response to the increased Ni doses applied to the soil. The amounts of Ni recovered by Mehlich-3 and DTPA from the Red-Yellow Latossol increased as a function of the increase in the soil applied Ni. The higher correlation was shown between the Ni extracted from soil with Mehlich-3 and the plant Ni contents and concentrations. The bigger difference between the results from Mehlich-3 and DTPA soil extraction was shown in the treatments with AN added to soil.O foco dos estudos sobre Ni, em geral, está voltado para os seus efeitos tóxicos às plantas, o estudo de como as plantas hiperacumuladoras de Ni toleram suas altas concentrações é uma das principais abordagens. Em anos recentes esse foco tem sido mudado dando-se mais atenção à participação do Ni na nutrição das plantas. Deve-se ressaltar que a função do Ni nas plantas está relacionada ao metabolismo do N, uma vez que o Ni tem papel essencial na atividade da urease, enzima que desdobra a uréia em NH3 e CO2. O objetivo deste trabalho foi avaliar o crescimento de plantas de alface (Lactuca sativa L.) em resposta à adubação com Ni via solo; avaliar os efeitos das doses de Ni sobre a atividade da urease em plantas que receberam uréia (Ur) (CO(NH2)2) ou nitrato de amônio (NA) (NH4NO3) como fontes de N; comparar a eficiência dos extratores Mehlich-3 e DTPA na determinação do Ni disponível em Latossolo Vermelho-Amarelo. O experimento foi constituído de fatorial 2 x 8 (2 fontes de N: Ur e NA; 8 doses de Ni: 0, 2, 4, 8, 12, 16, 24 e 32 mg/dm3) com cinco repetições, no delineamento em blocos casualizados, sendo a unidade experimental um vaso plástico contendo 3 dm3 de solo e duas plantas de alface. O N (200 mg/dm3) foi suprido às plantas na forma de Ur ou NA, metade no plantio e metade em cobertura, e o Ni foi suprido na forma de NiCl2. Uma planta de alface, por vaso, foi colhida 63 d após a semeadura para obtenção da massa de matéria seca (MS) e análise da composição mineral. Uma semana depois, foi colhida a outra planta, com sua matéria fresca sendo analisada para determinação da atividade da urease pelo método do indofenol. Na matéria seca, foram determinados os teores de K, Ca, Mg, Mn, Cu, Zn , Ni (digestão nítrico- perclórica, dosagem por ICP-OES) e N pelo método do Kjeldahl. No solo, foi determinado Ni disponível (extração com Mehlich-3 ou DTPA, dosagem por Espectrofotometria de Absorção Atômica). Foi feita análise de variância e ajuste das curvas de regressão para os dados referentes à produção de matéria seca, atividade da urease e teores dos elementos no solo e nas plantas para mostrar os efeitos das doses de Ni dentro das fontes de N. Foi feita análise de correlação entre os teores e conteúdos do Ni na parte aérea das plantas de alface com os teores de Ni extraídos do solo por Mehlich-3 e DTPA. Também ajustaram-se equações de regressão linear dos conteúdos de Ni nas plantas de alface e dos teores dos metais recuperados pelos dois extratores, em função das doses de Ni aplicadas ao solo. Na produção de MS da parte aérea das plantas houve diferença significativa devido às fontes de N (médias iguais a 5,07 g/pl para os tratamentos com NA e 4,20 g/pl para os tratamentos com Ur) enquanto que para as doses de Ni não houve resposta significativa. A atividade da urease aumentou significativamente com a adição das doses de Ni ao solo. Já para as fontes de N (NA ou Ur), não houve resposta com diferença significativa das plantas de alface quanto à atividade da urease. Nos tratamentos com NA houve menor teor de N total nas plantas, o que provavelmente se deveu a um efeito de diluição. Já para o suprimento de Ni ao solo, não houve resposta do teor de N total das plantas às doses desse micronutriente aplicado ao solo. Com o aumento das doses de Ni aplicadas ao Latossolo Vermelho Amarelo, houve aumento nas quantidades de Ni recuperadas pelos extratores Mehlich-3 e DTPA. A maior correlação foi obtida entre os teores de Ni extraídos com Mehlich-3 e os teores e conteúdos de Ni nas plantas, sendo que a maior diferença entre os extratores foi encontrada nos tratamentos com aplicação de NA.Fundação de Amparo a Pesquisa do Estado de Minas Geraisapplication/pdfporUniversidade Federal de ViçosaMestrado em Solos e Nutrição de PlantasUFVBRFertilidade do solo e nutrição de plantas; Gênese, Morfologia e Classificação, Mineralogia, Química,NiquelUreaseAlfaceNickelUreaseLettuceCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLOAtividade da urease e crescimento de alface no solo em resposta a níquelUrease activity and lettuce growth in response to soil added nickelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf328878https://locus.ufv.br//bitstream/123456789/5416/1/texto%20completo.pdfd346f15ea9b0761f5f95737e7a3028d4MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain83822https://locus.ufv.br//bitstream/123456789/5416/2/texto%20completo.pdf.txt0290c08332040aba323c93ce3679d938MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3663https://locus.ufv.br//bitstream/123456789/5416/3/texto%20completo.pdf.jpg184cdb85af47f95f371e2eb9a8eb5029MD53123456789/54162016-04-11 23:01:58.107oai:locus.ufv.br:123456789/5416Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-12T02:01:58LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Atividade da urease e crescimento de alface no solo em resposta a níquel
dc.title.alternative.eng.fl_str_mv Urease activity and lettuce growth in response to soil added nickel
title Atividade da urease e crescimento de alface no solo em resposta a níquel
spellingShingle Atividade da urease e crescimento de alface no solo em resposta a níquel
Oliveira, Thomas Carlos de
Niquel
Urease
Alface
Nickel
Urease
Lettuce
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO
title_short Atividade da urease e crescimento de alface no solo em resposta a níquel
title_full Atividade da urease e crescimento de alface no solo em resposta a níquel
title_fullStr Atividade da urease e crescimento de alface no solo em resposta a níquel
title_full_unstemmed Atividade da urease e crescimento de alface no solo em resposta a níquel
title_sort Atividade da urease e crescimento de alface no solo em resposta a níquel
author Oliveira, Thomas Carlos de
author_facet Oliveira, Thomas Carlos de
author_role author
dc.contributor.author.fl_str_mv Oliveira, Thomas Carlos de
dc.contributor.advisor-co1.fl_str_mv Venegas, Victor Hugo Alvarez
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727865T0
dc.contributor.advisor-co2.fl_str_mv Cecon, Paulo Roberto
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788114T5
dc.contributor.advisor1.fl_str_mv Fontes, Renildes Lúcio Ferreira
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781182P3
dc.contributor.referee1.fl_str_mv Cantarutti, Reinaldo Bertola
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780430A1
dc.contributor.referee2.fl_str_mv Rezende, Sebastião Tavares de
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787599A3
contributor_str_mv Venegas, Victor Hugo Alvarez
Cecon, Paulo Roberto
Fontes, Renildes Lúcio Ferreira
Cantarutti, Reinaldo Bertola
Rezende, Sebastião Tavares de
dc.subject.por.fl_str_mv Niquel
Urease
Alface
topic Niquel
Urease
Alface
Nickel
Urease
Lettuce
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO
dc.subject.eng.fl_str_mv Nickel
Urease
Lettuce
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO
description The studies with Ni in soils and plants, in general, are more related to its toxicity effects to plants with emphasis in the hability of Ni hyperacumulator plants to tolerate high Ni concentrations. In recent years, this approach has been changed with more attention to the Ni participation in the plant mineral nutrition. It has to be noted that Ni is related to the plant N metabolism, due to its participation in the activity of the enzyme urease which mediate the transformation of urea in NH3 and CO2. The aim of this research was to evaluate the growth of lettuce (Lactuca sativa L.) in response to soil Ni fertilization; the effects of Ni in the urease activity in plants supplied with urea (CO(NH2)2) and ammonium nitrate (NH4NO3) as N sources; to compare the efficiency of Mehlich-3 and DTPA extractions in the determination of Ni availability in a Red-Yellow Latossol (Oxysol). The experiment was set as a 2 x 8 (2 N sources: CO (NH2)2 and NH4NO3; 8 Ni doses: 0, 2, 4, 8, 12, 16, 24 and 32 mg/dm3) with 5 replications, in an entirely ramdomly block design. The experimental unity was a 3 dm3 plastic vase with soil, treatments and two lettuce plants. Nitrogen (200 mg/dm3) was supplied as urea (Ur) or ammonium nitrate (AN), half in the seedling and half surface dressed, and Ni was supplied as NiCl2 at the seedling. One lettuce plant, per vase, was harvested 63 days after seedling and oven dried for the determination of the dry matter mass (DM) and analysis of the plant mineral composition. One week later, the other plant was harvested and its fresh matter used for the determination of urease activity by the method of indophenols. In the dry matter it was determined the dry matter contents of K, Ca, Mg, Mn, Cu, Zn, Ni (nitric-perchloric digestion and ICP-OES dosage) and N by the Kjeldahl method. In the soil it was determined the available Ni by Mehlich-3 and DTPA extractions and AAS dosage. The analysis of variance of the data was made and regression equations were adjusted for dry matter production, urease activity, soil nutrient and plant nutrient concentrations, as a function of the Ni doses applied to soil, for each N source, Ur and NA. Analysis of correlation was made between the concentrations and contents of Ni in the lettuce leaves and soil extracted Ni by Mehlich-3 and DTPA. Additionally, linear regression equations were adjusted for the plant Ni contents and the Mehlich-3 and DTPA soil recovered Ni, as a function of the Ni doses applied to soil. There was significant difference in the dry matter yield between theplants supplied by different N sources (5.07 g/plant mean for NA supplied plants and 4.20 g/plant mean for Ur supplied plants). For Ni doses applied to soil there was no significant difference in the lettuce dry matter yield whereas for the urease activity there was increase as Ni was added to soil. There was no difference between the N sources (Ur and AN) as related to the urease activity in the lettuce leaves. Smaller N concentration was detected in plants grown under the AN soil treatments, which was probably caused by a dilution effect. The total N in the plants was uniform in response to the increased Ni doses applied to the soil. The amounts of Ni recovered by Mehlich-3 and DTPA from the Red-Yellow Latossol increased as a function of the increase in the soil applied Ni. The higher correlation was shown between the Ni extracted from soil with Mehlich-3 and the plant Ni contents and concentrations. The bigger difference between the results from Mehlich-3 and DTPA soil extraction was shown in the treatments with AN added to soil.
publishDate 2009
dc.date.available.fl_str_mv 2009-08-07
2015-03-26T13:53:07Z
dc.date.issued.fl_str_mv 2009-03-31
dc.date.accessioned.fl_str_mv 2015-03-26T13:53:07Z
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dc.identifier.citation.fl_str_mv OLIVEIRA, Thomas Carlos de. Urease activity and lettuce growth in response to soil added nickel. 2009. 50 f. Dissertação (Mestrado em Fertilidade do solo e nutrição de plantas; Gênese, Morfologia e Classificação, Mineralogia, Química,) - Universidade Federal de Viçosa, Viçosa, 2009.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/5416
identifier_str_mv OLIVEIRA, Thomas Carlos de. Urease activity and lettuce growth in response to soil added nickel. 2009. 50 f. Dissertação (Mestrado em Fertilidade do solo e nutrição de plantas; Gênese, Morfologia e Classificação, Mineralogia, Química,) - Universidade Federal de Viçosa, Viçosa, 2009.
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