Chromomeric pattern of maize pachytene chromosomes after trypsin treatment
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://www.locus.ufv.br/handle/123456789/12077 |
Resumo: | The bivalent chromomeres of maize were analyzed by means of enzymatic treatment with trypsin. The aim was to verify if the effect of swelling observed in human meiotic chromosomes also occurs in these bivalents, and if this morphological change allows a greater visual resolution of the chromomeric pattern. Bivalents treated with trypsin showed a structure with sequential and linear distribution of small rings. Comparisons between bivalent morphology, treated and non-treated, indicated a collinearity among the sequences of rings and the chromomeres. The results obtained were considered useful to analyze and to make a chromomere map. This treatment can also provide cytological evidence that may help to understand the pairing process. It was concluded that the ring-shape was caused by trypsin-induced “disjoining” just in the chromomeric but not in the interchromomeric regions. |
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Caixeta, Evelie TexeiraCarvalho, Carlos Roberto de2017-10-16T12:50:59Z2017-10-16T12:50:59Z2001-01-091601-522310.1111/j.1601-5223.2000.00183.xhttp://www.locus.ufv.br/handle/123456789/12077The bivalent chromomeres of maize were analyzed by means of enzymatic treatment with trypsin. The aim was to verify if the effect of swelling observed in human meiotic chromosomes also occurs in these bivalents, and if this morphological change allows a greater visual resolution of the chromomeric pattern. Bivalents treated with trypsin showed a structure with sequential and linear distribution of small rings. Comparisons between bivalent morphology, treated and non-treated, indicated a collinearity among the sequences of rings and the chromomeres. The results obtained were considered useful to analyze and to make a chromomere map. This treatment can also provide cytological evidence that may help to understand the pairing process. It was concluded that the ring-shape was caused by trypsin-induced “disjoining” just in the chromomeric but not in the interchromomeric regions.engHereditas133: 183-187 (2000) , janeiro 2001Trypsin treatmentChromomeric patternChromomeric pattern of maize pachytene chromosomes after trypsin treatmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALCaixeta_et_al-2001-Hereditas.pdfCaixeta_et_al-2001-Hereditas.pdftexto completoapplication/pdf524582https://locus.ufv.br//bitstream/123456789/12077/1/Caixeta_et_al-2001-Hereditas.pdf0966fc0eb5835303642c63b34772ab93MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/12077/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILCaixeta_et_al-2001-Hereditas.pdf.jpgCaixeta_et_al-2001-Hereditas.pdf.jpgIM Thumbnailimage/jpeg4817https://locus.ufv.br//bitstream/123456789/12077/3/Caixeta_et_al-2001-Hereditas.pdf.jpg41ad051eab0483904ce6dd64c138a4c7MD53123456789/120772017-10-16 22:00:30.932oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-10-17T01:00:30LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Chromomeric pattern of maize pachytene chromosomes after trypsin treatment |
title |
Chromomeric pattern of maize pachytene chromosomes after trypsin treatment |
spellingShingle |
Chromomeric pattern of maize pachytene chromosomes after trypsin treatment Caixeta, Evelie Texeira Trypsin treatment Chromomeric pattern |
title_short |
Chromomeric pattern of maize pachytene chromosomes after trypsin treatment |
title_full |
Chromomeric pattern of maize pachytene chromosomes after trypsin treatment |
title_fullStr |
Chromomeric pattern of maize pachytene chromosomes after trypsin treatment |
title_full_unstemmed |
Chromomeric pattern of maize pachytene chromosomes after trypsin treatment |
title_sort |
Chromomeric pattern of maize pachytene chromosomes after trypsin treatment |
author |
Caixeta, Evelie Texeira |
author_facet |
Caixeta, Evelie Texeira Carvalho, Carlos Roberto de |
author_role |
author |
author2 |
Carvalho, Carlos Roberto de |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Caixeta, Evelie Texeira Carvalho, Carlos Roberto de |
dc.subject.pt-BR.fl_str_mv |
Trypsin treatment Chromomeric pattern |
topic |
Trypsin treatment Chromomeric pattern |
description |
The bivalent chromomeres of maize were analyzed by means of enzymatic treatment with trypsin. The aim was to verify if the effect of swelling observed in human meiotic chromosomes also occurs in these bivalents, and if this morphological change allows a greater visual resolution of the chromomeric pattern. Bivalents treated with trypsin showed a structure with sequential and linear distribution of small rings. Comparisons between bivalent morphology, treated and non-treated, indicated a collinearity among the sequences of rings and the chromomeres. The results obtained were considered useful to analyze and to make a chromomere map. This treatment can also provide cytological evidence that may help to understand the pairing process. It was concluded that the ring-shape was caused by trypsin-induced “disjoining” just in the chromomeric but not in the interchromomeric regions. |
publishDate |
2001 |
dc.date.issued.fl_str_mv |
2001-01-09 |
dc.date.accessioned.fl_str_mv |
2017-10-16T12:50:59Z |
dc.date.available.fl_str_mv |
2017-10-16T12:50:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
10.1111/j.1601-5223.2000.00183.x http://www.locus.ufv.br/handle/123456789/12077 |
dc.identifier.issn.none.fl_str_mv |
1601-5223 |
identifier_str_mv |
1601-5223 10.1111/j.1601-5223.2000.00183.x |
url |
http://www.locus.ufv.br/handle/123456789/12077 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
133: 183-187 (2000) , janeiro 2001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hereditas |
publisher.none.fl_str_mv |
Hereditas |
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reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
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UFV |
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LOCUS Repositório Institucional da UFV |
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