Morfologia, imuno-histoquímica e aspectos ultraestruturais das células imunorreativas à insulina no intestino delgado do gambá Didelphis aurita (Wied-Neuwied, 1826) adulto

• Autor(a) principal: Basile, Daniel Raul Santurio
• Data de Publicação: 2011
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/248
• topic: Células imunorreativas; Insulina; Intestino delgado; Gambá; Immunoreactive cells; Insulin; Small intestine; Opossum; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

This research had as its objective to describe the morphologic, histochemical and immunohistochemical aspects of the small intestine of the opossum Didelphis aurita (Wied-Neuwied, 1826) adult, as well as the ultrastructural characterisitics of the secretory granules of insuline immunoreactive cells in this animal. The biogenesis of insulin granules and its classification, as well as physiological activity in insulin producer regions, are areas of continuous investigation in the control of diabetes and other pathologies. The material of study consisted of seven exemplars of opossum Didelphis aurita, males, with average weight of 1,03±0,14 kg. The number of insulin immunoreactive cells (IRI) per mm² in the small intestine of opossum D. aurita was different due to the analyzed segment. According to the multiple comparison of ordered averages, the number of IRI cells per mm² in the duodenum and jejunum was larger than in the ileum (p<0,05). The small intestine of the adult opossum D. aurita presented differences in the distribution of IRI cells, being them difuses along the intestinal epithelium and with higher concentration in the crypts and reduction in the villus. The IRI cells are characterized by the presence of secretor granules, by stretchered pyramidal morphology and by the localization among different cells. Additionally, it was possible to affirm that they presented morphology similar to beta cells of pancreas, nevertheless with larger granular volume and diameter. In the opossum Didelphis aurita, were observed values between 2,7 and 2,5 5 &#956;IU/mL of plasmatic insuline, values which were inferior to the ones verified in other mammals and humans. The results related to insulinemia, in this specie, suggest the maintenance of homeostasis of glucose in face to extrapancreatic insulin secretor activity. In the morphologic and histochemical studies of the argirophile and argentafins in the small intestine, it was demonstrated that argirophile cells were more numerous related to argentafins and that both were characterized by having pyramidal conformation ovoid nucleus, being the type closed or open, presenting granular aspect and being more frequent in intestinal glands. It was quantified a greater number of argirophile cells per mm² than argentafins, and the ileum was the segment with the shortest number of quantified endocrinous cells. The height and thickness of intestinal villus, as well as the density of absorption area of the intestinal epithelium of adult opossums D. aurita did not vary among the segments. The thickness of the muscular layer of the jejunum was thicker than the ileum, and the other comparisons did not present significant morphological differences.