In vitro evaluation of novel crude extracts produced by actinobacteria for modulation of ruminal fermentation
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://locus.ufv.br//handle/123456789/30721 https://doi.org/10.1590/rbz4820190066 |
Resumo: | We evaluated the inclusion of two crude extracts produced by Streptomyces genus on in vitro dry matter and organic matter digestibility (IVDMD; IVOMD), cumulative gas production, volatile fatty acids (VFA), and methane (CH4 ) and ammoniacal nitrogen (NH3 -N) concentration. The experimental design was randomized blocks with three blocks and four treatments: AMC (1.2 mg/25 mL ruminal inoculum), Caat (1.2 mg/25 mL ruminal inoculum), negative control (no inclusion of extracts), and positive control (sodium monensin, 1.7 mg/25 mL ruminal inoculum). Ruminal fluid samples were collected from three multiparous Holstein dairy cows fitted with ruminal cannula and incubated in a 24-h fermentation assay. There was no effect of crude extract inclusion in comparison with negative control on cumulative gas production in 24 h. However, cumulative gas production was lower when Caat extract was included in comparison with AMC inclusion. The Caat inclusion increased propionic acid concentration and reduced the concentration of butyric acid and acetate:propionate (A:P) ratio in relation to negative control. The CH4 concentration was lower with Caat inclusion in relation to AMC, and the ratio of CH4 concentration to digestible dry matter was lower in the negative control compared with all additives. Caat inclusion decreased the NH3 -N concentration, and IVDMD was not altered compared with negative control. Additionally, the inclusion of Caat crude extract increased propionic acid concentration and reduced butyric acid concentration and A:P ratio, without reducing the IVDMD and IVOMD. Caat extract modulates rumen fermentation, increasing available energy and decreasing gas production without causing changes in dry matter and organic matter digestibility |
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Alves, Bruna GomesMartins, Cristian Marlon de Magalhães RodriguesPeti, Ana Paula FerrantiMoraes, Luiz Alberto Beraldo deSantos, Marcos Veiga dos2023-04-14T14:02:49Z2023-04-14T14:02:49Z2019-11-06Alves, B. G.; Martins, C. M. M. R.; Peti, A. P. F.; Moraes, L. A. B. and Santos, M. V. 2019. In vitro evaluation of novel crude extracts produced by actinobacteria for modulation of ruminal fermentation. Revista Brasileira de Zootecnia 48:e201900661806-9290https://locus.ufv.br//handle/123456789/30721https://doi.org/10.1590/rbz4820190066We evaluated the inclusion of two crude extracts produced by Streptomyces genus on in vitro dry matter and organic matter digestibility (IVDMD; IVOMD), cumulative gas production, volatile fatty acids (VFA), and methane (CH4 ) and ammoniacal nitrogen (NH3 -N) concentration. The experimental design was randomized blocks with three blocks and four treatments: AMC (1.2 mg/25 mL ruminal inoculum), Caat (1.2 mg/25 mL ruminal inoculum), negative control (no inclusion of extracts), and positive control (sodium monensin, 1.7 mg/25 mL ruminal inoculum). Ruminal fluid samples were collected from three multiparous Holstein dairy cows fitted with ruminal cannula and incubated in a 24-h fermentation assay. There was no effect of crude extract inclusion in comparison with negative control on cumulative gas production in 24 h. However, cumulative gas production was lower when Caat extract was included in comparison with AMC inclusion. The Caat inclusion increased propionic acid concentration and reduced the concentration of butyric acid and acetate:propionate (A:P) ratio in relation to negative control. The CH4 concentration was lower with Caat inclusion in relation to AMC, and the ratio of CH4 concentration to digestible dry matter was lower in the negative control compared with all additives. Caat inclusion decreased the NH3 -N concentration, and IVDMD was not altered compared with negative control. Additionally, the inclusion of Caat crude extract increased propionic acid concentration and reduced butyric acid concentration and A:P ratio, without reducing the IVDMD and IVOMD. Caat extract modulates rumen fermentation, increasing available energy and decreasing gas production without causing changes in dry matter and organic matter digestibilityengBrazilian Journal of Animal ScienceR. Bras. Zootec., 48:e20190066, 2019Creative Commons Attribution Licenseinfo:eu-repo/semantics/openAccessactinobacteriaextractsin vitro fermentationmonensinIn vitro evaluation of novel crude extracts produced by actinobacteria for modulation of ruminal fermentationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINAL1806-9290-rbz-48-e20190066.pdf1806-9290-rbz-48-e20190066.pdfartigoapplication/pdf344806https://locus.ufv.br//bitstream/123456789/30721/1/1806-9290-rbz-48-e20190066.pdf404b77dabbe12e097491c3bd1f43ead5MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/30721/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/307212023-04-14 11:02:50.156oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452023-04-14T14:02:50LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
In vitro evaluation of novel crude extracts produced by actinobacteria for modulation of ruminal fermentation |
title |
In vitro evaluation of novel crude extracts produced by actinobacteria for modulation of ruminal fermentation |
spellingShingle |
In vitro evaluation of novel crude extracts produced by actinobacteria for modulation of ruminal fermentation Alves, Bruna Gomes actinobacteria extracts in vitro fermentation monensin |
title_short |
In vitro evaluation of novel crude extracts produced by actinobacteria for modulation of ruminal fermentation |
title_full |
In vitro evaluation of novel crude extracts produced by actinobacteria for modulation of ruminal fermentation |
title_fullStr |
In vitro evaluation of novel crude extracts produced by actinobacteria for modulation of ruminal fermentation |
title_full_unstemmed |
In vitro evaluation of novel crude extracts produced by actinobacteria for modulation of ruminal fermentation |
title_sort |
In vitro evaluation of novel crude extracts produced by actinobacteria for modulation of ruminal fermentation |
author |
Alves, Bruna Gomes |
author_facet |
Alves, Bruna Gomes Martins, Cristian Marlon de Magalhães Rodrigues Peti, Ana Paula Ferranti Moraes, Luiz Alberto Beraldo de Santos, Marcos Veiga dos |
author_role |
author |
author2 |
Martins, Cristian Marlon de Magalhães Rodrigues Peti, Ana Paula Ferranti Moraes, Luiz Alberto Beraldo de Santos, Marcos Veiga dos |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Alves, Bruna Gomes Martins, Cristian Marlon de Magalhães Rodrigues Peti, Ana Paula Ferranti Moraes, Luiz Alberto Beraldo de Santos, Marcos Veiga dos |
dc.subject.eng.fl_str_mv |
actinobacteria extracts in vitro fermentation monensin |
topic |
actinobacteria extracts in vitro fermentation monensin |
description |
We evaluated the inclusion of two crude extracts produced by Streptomyces genus on in vitro dry matter and organic matter digestibility (IVDMD; IVOMD), cumulative gas production, volatile fatty acids (VFA), and methane (CH4 ) and ammoniacal nitrogen (NH3 -N) concentration. The experimental design was randomized blocks with three blocks and four treatments: AMC (1.2 mg/25 mL ruminal inoculum), Caat (1.2 mg/25 mL ruminal inoculum), negative control (no inclusion of extracts), and positive control (sodium monensin, 1.7 mg/25 mL ruminal inoculum). Ruminal fluid samples were collected from three multiparous Holstein dairy cows fitted with ruminal cannula and incubated in a 24-h fermentation assay. There was no effect of crude extract inclusion in comparison with negative control on cumulative gas production in 24 h. However, cumulative gas production was lower when Caat extract was included in comparison with AMC inclusion. The Caat inclusion increased propionic acid concentration and reduced the concentration of butyric acid and acetate:propionate (A:P) ratio in relation to negative control. The CH4 concentration was lower with Caat inclusion in relation to AMC, and the ratio of CH4 concentration to digestible dry matter was lower in the negative control compared with all additives. Caat inclusion decreased the NH3 -N concentration, and IVDMD was not altered compared with negative control. Additionally, the inclusion of Caat crude extract increased propionic acid concentration and reduced butyric acid concentration and A:P ratio, without reducing the IVDMD and IVOMD. Caat extract modulates rumen fermentation, increasing available energy and decreasing gas production without causing changes in dry matter and organic matter digestibility |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019-11-06 |
dc.date.accessioned.fl_str_mv |
2023-04-14T14:02:49Z |
dc.date.available.fl_str_mv |
2023-04-14T14:02:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Alves, B. G.; Martins, C. M. M. R.; Peti, A. P. F.; Moraes, L. A. B. and Santos, M. V. 2019. In vitro evaluation of novel crude extracts produced by actinobacteria for modulation of ruminal fermentation. Revista Brasileira de Zootecnia 48:e20190066 |
dc.identifier.uri.fl_str_mv |
https://locus.ufv.br//handle/123456789/30721 |
dc.identifier.issn.none.fl_str_mv |
1806-9290 |
dc.identifier.doi.pt-BR.fl_str_mv |
https://doi.org/10.1590/rbz4820190066 |
identifier_str_mv |
Alves, B. G.; Martins, C. M. M. R.; Peti, A. P. F.; Moraes, L. A. B. and Santos, M. V. 2019. In vitro evaluation of novel crude extracts produced by actinobacteria for modulation of ruminal fermentation. Revista Brasileira de Zootecnia 48:e20190066 1806-9290 |
url |
https://locus.ufv.br//handle/123456789/30721 https://doi.org/10.1590/rbz4820190066 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
R. Bras. Zootec., 48:e20190066, 2019 |
dc.rights.driver.fl_str_mv |
Creative Commons Attribution License info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Creative Commons Attribution License |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Brazilian Journal of Animal Science |
publisher.none.fl_str_mv |
Brazilian Journal of Animal Science |
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reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
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LOCUS Repositório Institucional da UFV |
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