Extração de antocianinas dos frutos do açai da Mata Atlântica (Euterpe edulis Martius) e sua atuação nas atividades antioxidante e antiaterogênica em camundongos ApoE -/-

• Autor(a) principal: Novello, Alexandre Azevedo
• Data de Publicação: 2011
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/2758
• topic: Extração; Antocianinas; Atividade antioxidante; Açaí; Euterpe edulis; Aterosclerose; ApoE -/-; Extraction; Anthocyanins, Antioxidant activity; Açai; Euterpe edulis; Atherosclerosis; ApoE -/-; CNPQ::CIENCIAS DA SAUDE::NUTRICAO

Açaí is a fruit of the palm trees of the genus Euterpe Martius. In the Amazon region the açaí drink is obtained from the pulp of the fruits of açaizeiro (Euterpe oleracea Martius). In the region of the Atlantic Forest can be obtained from the açaí fruit from Juçara (Euterpe edulis Martius). This is a palm characteristic of the Atlantic Forest occurring from the Rio Grande do Sul to Bahia, with main distribution in the Atlantic coast. Currently, E. edulis is a palm species threatened with extinction. There are few reports of beneficial effects and composition of the fruits from this species. It has been argued that positive effect on health is related to the presence of unsaturated fatty acids, minerals and phenolic compounds such as anthocyanins in this fruit. In this study we tested the best solvent for the extraction of anthocyanins and evaluated their antioxidant and antiatherogenic effects by adding the extract of freeze-dried açaí pulp to the diet of ApoE-/- mice divided into four experimental groups. The group C+ (positive control) received the standard AIN-93M diet, the group 2% and 6% received extracts of açaí in the AIN-93M diet at 2% and 6% respectively, and the group SINV received the drug simvastatin on the estimated amount of 50mg/kg/day for mice. A fifth group, C57BL6 mice (negative control) received standard AIN-93M diet for 75 days of experiment. A reduction in the levels of antioxidant enzymes (superoxide dismutase and catalase) in the liver tissue of mice treated with açaí, suggesting that this activity may have been suppressed due to the synergistic effect of anthocyanins. There was no reduction in the evolution of atherosclerotic plaques for the treated groups compared to the positive control, but açaí was able to reduce glucose, total cholesterol and LDL, showing that there was no relationship between the plaques evolution and cholesterol levels during this treatment time. The results showed that the extract of freeze-dried açaí improved antioxidant activity, blood glucose and lipid profile, but was not able to minimize the development of atherosclerotic plaque in ApoE-/- mice during the treatment period.