Identificação de genes potencialmente envolvidos na interação tomateiro - Potyvirus

• Autor(a) principal: Zerbini, Poliane Alfenas
• Data de Publicação: 2006
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/1388
• topic: Interação PepYMV-tomateiro; Transcriptoma; Virus induced gene silincing (VIGS); Tomato-PepYMV interaction; Transcriptional regulations; Virus induced gene silincing (VIGS); CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL

During co-evolution between virus and host, a complex interaction has been developed involving several mechanisms of pathogen attack and host defense. Host defense responses cause up- and downward shifts in gene expression. However, the effects of viral infection in the host s gene expression profile are still poorly understood. With the objective of identifying differentially expressed genes in a virushost interaction, susceptible tomato plants were inoculated with the potyvirus Pepper yellow mosaic virus (PepYMV), and a subtractive library was constructed based on mRNA extracted from inoculated leaves at 72 h after inoculation. A total of 777 genes differentially expressed were identified, including genes involved in a number of plant defense responses as well as transcriptional regulators and signaling proteins, including catalase, aldolase, cystein proteases, heat shock proteins, polyubiquitin, proteassome subunits, proteins involved in gene silencing, among others. A total of 104 down-regulated genes were also identified, which likewise display homology with genes involved in several metabolic pathways. Differential expression of selected genes was validated by quantitative RT-PCR and macroarray analysis. The study of these genes, together with information on the viral infection cycle, provides a global view of the host factors used by the virus to synthesize its proteins and to infect new cells and tissues, as well as the responses from the host, in its attempt to contain or at least minimize the damage caused by the infection. Functional analysis of these genes will be necessary in order to determine their specific role in the interaction.