Hidroxiapatita associada à lignina no preenchimento de falha óssea experimental na tíbia de coelhos

Detalhes bibliográficos
Autor(a) principal: Vital, Cristiane Carneiro
Data de Publicação: 2005
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/5018
Resumo: The propose of this work was to evaluate synthetic hydroxyapatite with lignin in solid form as substitute in bone regeneration. Twenty rabbits, from New Zealand race, clinically healthy were used. The animals were divided in two groups with 10 animals each one. Group one received hydroxyapatite associated lignin and group two didn t received the implant. After customary anesthetic and surgical protocol in all the animals, a skin incision was made on lateral surface in the right pelvic member in their third middle. A circular bone fragment with 4,5 mm of diameter was retreated from the tibia in all groups, and the bone defect was filled up as a protocol for each treatment group. The animals were submitted to a daily clinical inspection to observe inflammatory reaction and painful sensibility. Radiographs were taken immediately after the surgery and at four, eight, 15, 30 and 60 days after surgery to evaluate bone healing. At same time, two animals per time period were sacrificed and fragments for histopathological evaluations through optical microscopy were collected. The samples were fixed in 10% formol and processed as customary technical in histopathology. The clinical results showed that most of the animals had a normal clinical evolution without infection or rejection of the implants. The results of the X-rays showed a decreasing of opacity on the bone defect until they look like regular bone in the groups that received hidroxyapatite. In the control group, the X-rays revealed increasing of opacity on the bone defect. The histological results showed normal evaluation of the bone regeneration in control group and in the treated one, although it was more precocious in the group that received hidroxyapatite until the 30th day observation. Therefore, the use of hidroxyapatite associated with lignin as bone substitute showed to be efficient, without inflammatory reaction and showing to be biocompatible.
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spelling Vital, Cristiane Carneirohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4755000E3Borges, Andrea Pacheco Batistahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767373D8Fonseca, Cláudio Césarhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780777E6Pompermayer, Luiz Gonzagahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4769212Z2Malm, Christinahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784181Y6Melo, Eliane Gonçalves dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4796951E22015-03-26T13:46:49Z2007-01-172015-03-26T13:46:49Z2005-03-21VITAL, Cristiane Carneiro. Hydroxyapatite associated to lignin in the filling of experimental bone defect in rabbit s tibia. 2005. 57 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2005.http://locus.ufv.br/handle/123456789/5018The propose of this work was to evaluate synthetic hydroxyapatite with lignin in solid form as substitute in bone regeneration. Twenty rabbits, from New Zealand race, clinically healthy were used. The animals were divided in two groups with 10 animals each one. Group one received hydroxyapatite associated lignin and group two didn t received the implant. After customary anesthetic and surgical protocol in all the animals, a skin incision was made on lateral surface in the right pelvic member in their third middle. A circular bone fragment with 4,5 mm of diameter was retreated from the tibia in all groups, and the bone defect was filled up as a protocol for each treatment group. The animals were submitted to a daily clinical inspection to observe inflammatory reaction and painful sensibility. Radiographs were taken immediately after the surgery and at four, eight, 15, 30 and 60 days after surgery to evaluate bone healing. At same time, two animals per time period were sacrificed and fragments for histopathological evaluations through optical microscopy were collected. The samples were fixed in 10% formol and processed as customary technical in histopathology. The clinical results showed that most of the animals had a normal clinical evolution without infection or rejection of the implants. The results of the X-rays showed a decreasing of opacity on the bone defect until they look like regular bone in the groups that received hidroxyapatite. In the control group, the X-rays revealed increasing of opacity on the bone defect. The histological results showed normal evaluation of the bone regeneration in control group and in the treated one, although it was more precocious in the group that received hidroxyapatite until the 30th day observation. Therefore, the use of hidroxyapatite associated with lignin as bone substitute showed to be efficient, without inflammatory reaction and showing to be biocompatible.O objetivo deste trabalho foi avaliar a hidroxiapatita sintética associada à lignina em forma de bloco, como substituto na regeneração óssea. Para tanto, foram utilizados vinte coelhos, clinicamente sadios, da raça Nova Zelândia. Os animais foram divididos em dois grupos, com 10 animais em cada. O grupo um recebeu hidroxiapatita associada à lignina e o grupo dois não recebeu o implante. Após a realização de protocolo anestésico rotineiro, foi feita uma incisão de pele no terço médio do membro pélvico direito, expondo a tíbia. Um fragmento ósseo circular de 4,5 mm de diâmetro foi retirado, com o auxílio de uma broca trefina, nos animais dos dois grupos, e o defeito foi preenchido de acordo com o protocolo de cada grupo. Os animais foram submetidos à avaliação clinica diária com o objetivo de se observar reação inflamatória e sensibilidade dolorosa. Foram realizadas radiografias imediatamente após o procedimento cirúrgico e aos quatro, oito, 15, 30 e 60 dias do pós-operatório para se avaliar o processo de regeneração óssea. Nas mesmas datas, exceto no dia da cirurgia, dois animais de cada grupo foram sacrificados objetivando a coleta de material para histologia. As amostras foram fixadas em formol 10%, tamponado, e processadas segundo técnicas rotineiras de histologia. Os resultados clínicos mostraram que a maioria dos animais teve evolução clinica normal, sem indícios de infecção ou de rejeição do implante. As radiografias mostraram, no grupo tratado, um decréscimo de radiopacidade do defeito, até que a imagem ficasse semelhante ao osso. Já no grupo controle, observou-se o inverso, ou seja, aumento gradual da radiopacidade do defeito. Os resultados histológicos mostraram evolução normal no processo de regeneração óssea nos dois grupos. Entretanto, nos animais tratados, o processo de reparação óssea se mostrou mais precoce nas observações de 15 e 30 dias, não havendo diferença entre os grupos na observação subseqüente. Com isto se conclui que a hidroxiapatita associada à lignina como substituto ósseo é eficiente, não causando reação inflamatória e se mostrando biocompatível.application/pdfporUniversidade Federal de ViçosaMestrado em Medicina VeterináriaUFVBRBiotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. deOrtopedia veterináriaHidroxiapatitaLigninaHydroxyapatiteLigninCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMALHidroxiapatita associada à lignina no preenchimento de falha óssea experimental na tíbia de coelhosHydroxyapatite associated to lignin in the filling of experimental bone defect in rabbit s tibiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf1216531https://locus.ufv.br//bitstream/123456789/5018/1/texto%20completo.pdf138862093563670993d56bc12bc4f483MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain74854https://locus.ufv.br//bitstream/123456789/5018/2/texto%20completo.pdf.txtfc5d4eae1e96299cd7f8a28c74fcd470MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3821https://locus.ufv.br//bitstream/123456789/5018/3/texto%20completo.pdf.jpg4ab652be7c02935ad4779e71569a37a1MD53123456789/50182016-04-11 23:07:01.85oai:locus.ufv.br:123456789/5018Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-12T02:07:01LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Hidroxiapatita associada à lignina no preenchimento de falha óssea experimental na tíbia de coelhos
dc.title.alternative.eng.fl_str_mv Hydroxyapatite associated to lignin in the filling of experimental bone defect in rabbit s tibia
title Hidroxiapatita associada à lignina no preenchimento de falha óssea experimental na tíbia de coelhos
spellingShingle Hidroxiapatita associada à lignina no preenchimento de falha óssea experimental na tíbia de coelhos
Vital, Cristiane Carneiro
Ortopedia veterinária
Hidroxiapatita
Lignina
Hydroxyapatite
Lignin
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL
title_short Hidroxiapatita associada à lignina no preenchimento de falha óssea experimental na tíbia de coelhos
title_full Hidroxiapatita associada à lignina no preenchimento de falha óssea experimental na tíbia de coelhos
title_fullStr Hidroxiapatita associada à lignina no preenchimento de falha óssea experimental na tíbia de coelhos
title_full_unstemmed Hidroxiapatita associada à lignina no preenchimento de falha óssea experimental na tíbia de coelhos
title_sort Hidroxiapatita associada à lignina no preenchimento de falha óssea experimental na tíbia de coelhos
author Vital, Cristiane Carneiro
author_facet Vital, Cristiane Carneiro
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4755000E3
dc.contributor.author.fl_str_mv Vital, Cristiane Carneiro
dc.contributor.advisor1.fl_str_mv Borges, Andrea Pacheco Batista
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767373D8
dc.contributor.referee1.fl_str_mv Fonseca, Cláudio César
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780777E6
dc.contributor.referee2.fl_str_mv Pompermayer, Luiz Gonzaga
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4769212Z2
dc.contributor.referee3.fl_str_mv Malm, Christina
dc.contributor.referee3Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784181Y6
dc.contributor.referee4.fl_str_mv Melo, Eliane Gonçalves de
dc.contributor.referee4Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4796951E2
contributor_str_mv Borges, Andrea Pacheco Batista
Fonseca, Cláudio César
Pompermayer, Luiz Gonzaga
Malm, Christina
Melo, Eliane Gonçalves de
dc.subject.por.fl_str_mv Ortopedia veterinária
Hidroxiapatita
Lignina
topic Ortopedia veterinária
Hidroxiapatita
Lignina
Hydroxyapatite
Lignin
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL
dc.subject.eng.fl_str_mv Hydroxyapatite
Lignin
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL
description The propose of this work was to evaluate synthetic hydroxyapatite with lignin in solid form as substitute in bone regeneration. Twenty rabbits, from New Zealand race, clinically healthy were used. The animals were divided in two groups with 10 animals each one. Group one received hydroxyapatite associated lignin and group two didn t received the implant. After customary anesthetic and surgical protocol in all the animals, a skin incision was made on lateral surface in the right pelvic member in their third middle. A circular bone fragment with 4,5 mm of diameter was retreated from the tibia in all groups, and the bone defect was filled up as a protocol for each treatment group. The animals were submitted to a daily clinical inspection to observe inflammatory reaction and painful sensibility. Radiographs were taken immediately after the surgery and at four, eight, 15, 30 and 60 days after surgery to evaluate bone healing. At same time, two animals per time period were sacrificed and fragments for histopathological evaluations through optical microscopy were collected. The samples were fixed in 10% formol and processed as customary technical in histopathology. The clinical results showed that most of the animals had a normal clinical evolution without infection or rejection of the implants. The results of the X-rays showed a decreasing of opacity on the bone defect until they look like regular bone in the groups that received hidroxyapatite. In the control group, the X-rays revealed increasing of opacity on the bone defect. The histological results showed normal evaluation of the bone regeneration in control group and in the treated one, although it was more precocious in the group that received hidroxyapatite until the 30th day observation. Therefore, the use of hidroxyapatite associated with lignin as bone substitute showed to be efficient, without inflammatory reaction and showing to be biocompatible.
publishDate 2005
dc.date.issued.fl_str_mv 2005-03-21
dc.date.available.fl_str_mv 2007-01-17
2015-03-26T13:46:49Z
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dc.identifier.citation.fl_str_mv VITAL, Cristiane Carneiro. Hydroxyapatite associated to lignin in the filling of experimental bone defect in rabbit s tibia. 2005. 57 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2005.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/5018
identifier_str_mv VITAL, Cristiane Carneiro. Hydroxyapatite associated to lignin in the filling of experimental bone defect in rabbit s tibia. 2005. 57 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2005.
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