Potencial do isotilcianato de alilo no controle de Sclerotium rolfsii e Sclerotinia sclerotiorum

Detalhes bibliográficos
Autor(a) principal: Schurt, Daniel Augusto
Data de Publicação: 2006
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/4447
Resumo: The losses caused by fungi in the nursery of seedling production are high if the planting substrate is not treated for their eradication. Transplanting infected seedlings to field causes disease in the field besides infesting the soil with pathogens transported by the disease seedling, where the disease control becomes economically unviable. The objective of this study was evaluate the effect of the, allyl isothiocyanate (AITC) for the soil treatment, to control Sclerotium rolfsii and Sclerotinia sclerotiorum and also to evaluate its effect on the general soil microbiota. The study about diffusion of AITC vapors in the soil showed that AITC diffused downwards better in dry soils (detected up to 15 cm of depth) than in moist soils, where it was detected up to 10 and 7,5 cm depth at soil moisture of 17 % and 22% respectively. Irrigating soil with water containing AITC to provide 150 µL/kg soil reduced the viability of the scletoria of S. rolfsii and S. sclerotiorum to a depth of 7,5 cm. In vitro, AITC vapors in the concentration of 150 µL/L killed 80% of concentration of AITC, in vitro or in soil, delayed sclerotial germination in the first 48 hours, and full germination potential was observed only after 120 h. The AITC effect on scletoria viality was slightly greater the air-dry soil compared to the soils, however from the practical point of view the soil moisture up to 20% and the high temperature did not affect the influence of AITC on sclerotial viability. In field conditions the used AITC at the rate of 8,86 ml/m2 killed 98% sclerotia of S. rolfsii when the plots were covered with a plastic mulch after the application. Soil treatment with AITC also reduced the microbial activity, changed significantly depending upon the soil conditions, but in general, the populations of actinomycetes, bacteria and fungi decreased.
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spelling Schurt, Daniel Augustohttp://lattes.cnpq.br/6986086365325708Dhingra, Onkar Devhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788051H5Oliveira, Rosângela D arc de Limahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788026Z3Jham, Gulab Newandramhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783775P0Rodrigues, Fabrício de ávilahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4709080E6Oliveira, José Rogério dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785966E62015-03-26T13:37:56Z2007-10-152015-03-26T13:37:56Z2006-03-20SCHURT, Daniel Augusto. Potential do allyl isothiocyanate to control Sclerotium rolfsii and Sclerotinia sclerotiorum. 2006. 57 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2006.http://locus.ufv.br/handle/123456789/4447The losses caused by fungi in the nursery of seedling production are high if the planting substrate is not treated for their eradication. Transplanting infected seedlings to field causes disease in the field besides infesting the soil with pathogens transported by the disease seedling, where the disease control becomes economically unviable. The objective of this study was evaluate the effect of the, allyl isothiocyanate (AITC) for the soil treatment, to control Sclerotium rolfsii and Sclerotinia sclerotiorum and also to evaluate its effect on the general soil microbiota. The study about diffusion of AITC vapors in the soil showed that AITC diffused downwards better in dry soils (detected up to 15 cm of depth) than in moist soils, where it was detected up to 10 and 7,5 cm depth at soil moisture of 17 % and 22% respectively. Irrigating soil with water containing AITC to provide 150 µL/kg soil reduced the viability of the scletoria of S. rolfsii and S. sclerotiorum to a depth of 7,5 cm. In vitro, AITC vapors in the concentration of 150 µL/L killed 80% of concentration of AITC, in vitro or in soil, delayed sclerotial germination in the first 48 hours, and full germination potential was observed only after 120 h. The AITC effect on scletoria viality was slightly greater the air-dry soil compared to the soils, however from the practical point of view the soil moisture up to 20% and the high temperature did not affect the influence of AITC on sclerotial viability. In field conditions the used AITC at the rate of 8,86 ml/m2 killed 98% sclerotia of S. rolfsii when the plots were covered with a plastic mulch after the application. Soil treatment with AITC also reduced the microbial activity, changed significantly depending upon the soil conditions, but in general, the populations of actinomycetes, bacteria and fungi decreased.As perdas causadas por fungos nas sementeiras e nos viveiros de produção de mudas são altas se o substrato não sofrer tratamento para a sua erradicação. O transplantio de mudas infectadas provoca doença além de infestar o solo com patógenos transportados pelas mudas, tornando-se economicamente inviável o controle do patógeno. O objetivo foi avaliar o efeito do isotiocianato de alilo (ITCA), para o tratamento de solo, visando ao controle de patógenos (Sclerotium rolfsii e Sclerotinia sclerotiorum) e avaliar seu efeito na microbiota geral do solo. Estudando a difusão descendente do ITCA no solo em tubos de PVC, coletou-se os vapores de ITCA na massa do solo em diferentes umidades e profundidades. Foi observado que o ITCA difundiu-se melhor em solos secos (15 cm de profundidade) que em solos com 17% (10 cm) e 22% (7,5 cm) umidade. A utilização do ITCA na água da irrigação diminuiu a viabilidade dos escleródios de S. rolfsii e S. sclerotiorum na profundidade de 7,5 cm em 95 % com ITCA na concentração de 150 µL/kg de solo. Em condições in vitro o ITCA na concentração de 150 µL/L matou 80% dos escleródios de S. rolfsii e S. sclerotiorum com 4 dias de exposição aos vapores de ITCA. O efeito do ITCA provocou um atraso na germinação dos escleródios, na concentração de 50 µL/L nas primeiras 48 horas, a germinação dos escleródios foram de 3,7 e 10% para S. rolfsii e S. sclerotiorum, respectivamente. No solo seco ao ar, foi observado um maior efeito do ITCA na mortalidade dos escleródios, que em solos úmidos (17 e 22% umidade) a mudança de umidade ou temperatura influenciaram na viabilidade dos escleródios. Em condições de campo, sem a lona a dose utilizada de 8,86 ml/m2 de ITCA controlou os escleródios de S. rolfsii, mas com a utilização de lona a mortalidade dos escleródios foi de 98%. Foi observado que a utilização de ITCA no solo diminuiu a atividade microbiana dependendo das condições de solo. A comunidade microbiana no solo, actinomicetos, bactérias e fungos sofreram mudanças com a adição do ITCA no solo. A unidades formadoras de colônias (ufc s) de actinomicetos e bactérias diminuíram, dependendo das condições do solo e ufc s de fungos também diminuíram com a adição do ITCA. A utilização no campo do ITCA aumentou o número de ufc s de bactérias e actinomicetos e diminuiu o ufc s de fungos.Fundação de Amparo a Pesquisa do Estado de Minas Geraisapplication/pdfporUniversidade Federal de ViçosaMestrado em FitopatologiaUFVBREtiologia; Epidemiologia; ControleÓleo mostardaFungoControleFungiControlCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIAPotencial do isotilcianato de alilo no controle de Sclerotium rolfsii e Sclerotinia sclerotiorumPotential do allyl isothiocyanate to control Sclerotium rolfsii and Sclerotinia sclerotioruminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf270927https://locus.ufv.br//bitstream/123456789/4447/1/texto%20completo.pdf2b5f4fb095ba6214b6028293429c9823MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain112239https://locus.ufv.br//bitstream/123456789/4447/2/texto%20completo.pdf.txt45c882e58e34ff168d69c9505ac0bfb6MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3562https://locus.ufv.br//bitstream/123456789/4447/3/texto%20completo.pdf.jpg5f4228efcf2d850444ba261cfd5fbc8cMD53123456789/44472016-04-10 23:10:16.799oai:locus.ufv.br:123456789/4447Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:10:16LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Potencial do isotilcianato de alilo no controle de Sclerotium rolfsii e Sclerotinia sclerotiorum
dc.title.alternative.eng.fl_str_mv Potential do allyl isothiocyanate to control Sclerotium rolfsii and Sclerotinia sclerotiorum
title Potencial do isotilcianato de alilo no controle de Sclerotium rolfsii e Sclerotinia sclerotiorum
spellingShingle Potencial do isotilcianato de alilo no controle de Sclerotium rolfsii e Sclerotinia sclerotiorum
Schurt, Daniel Augusto
Óleo mostarda
Fungo
Controle
Fungi
Control
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
title_short Potencial do isotilcianato de alilo no controle de Sclerotium rolfsii e Sclerotinia sclerotiorum
title_full Potencial do isotilcianato de alilo no controle de Sclerotium rolfsii e Sclerotinia sclerotiorum
title_fullStr Potencial do isotilcianato de alilo no controle de Sclerotium rolfsii e Sclerotinia sclerotiorum
title_full_unstemmed Potencial do isotilcianato de alilo no controle de Sclerotium rolfsii e Sclerotinia sclerotiorum
title_sort Potencial do isotilcianato de alilo no controle de Sclerotium rolfsii e Sclerotinia sclerotiorum
author Schurt, Daniel Augusto
author_facet Schurt, Daniel Augusto
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/6986086365325708
dc.contributor.author.fl_str_mv Schurt, Daniel Augusto
dc.contributor.advisor1.fl_str_mv Dhingra, Onkar Dev
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788051H5
dc.contributor.referee1.fl_str_mv Oliveira, Rosângela D arc de Lima
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788026Z3
dc.contributor.referee2.fl_str_mv Jham, Gulab Newandram
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783775P0
dc.contributor.referee3.fl_str_mv Rodrigues, Fabrício de ávila
dc.contributor.referee3Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4709080E6
dc.contributor.referee4.fl_str_mv Oliveira, José Rogério de
dc.contributor.referee4Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785966E6
contributor_str_mv Dhingra, Onkar Dev
Oliveira, Rosângela D arc de Lima
Jham, Gulab Newandram
Rodrigues, Fabrício de ávila
Oliveira, José Rogério de
dc.subject.por.fl_str_mv Óleo mostarda
Fungo
Controle
topic Óleo mostarda
Fungo
Controle
Fungi
Control
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
dc.subject.eng.fl_str_mv Fungi
Control
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
description The losses caused by fungi in the nursery of seedling production are high if the planting substrate is not treated for their eradication. Transplanting infected seedlings to field causes disease in the field besides infesting the soil with pathogens transported by the disease seedling, where the disease control becomes economically unviable. The objective of this study was evaluate the effect of the, allyl isothiocyanate (AITC) for the soil treatment, to control Sclerotium rolfsii and Sclerotinia sclerotiorum and also to evaluate its effect on the general soil microbiota. The study about diffusion of AITC vapors in the soil showed that AITC diffused downwards better in dry soils (detected up to 15 cm of depth) than in moist soils, where it was detected up to 10 and 7,5 cm depth at soil moisture of 17 % and 22% respectively. Irrigating soil with water containing AITC to provide 150 µL/kg soil reduced the viability of the scletoria of S. rolfsii and S. sclerotiorum to a depth of 7,5 cm. In vitro, AITC vapors in the concentration of 150 µL/L killed 80% of concentration of AITC, in vitro or in soil, delayed sclerotial germination in the first 48 hours, and full germination potential was observed only after 120 h. The AITC effect on scletoria viality was slightly greater the air-dry soil compared to the soils, however from the practical point of view the soil moisture up to 20% and the high temperature did not affect the influence of AITC on sclerotial viability. In field conditions the used AITC at the rate of 8,86 ml/m2 killed 98% sclerotia of S. rolfsii when the plots were covered with a plastic mulch after the application. Soil treatment with AITC also reduced the microbial activity, changed significantly depending upon the soil conditions, but in general, the populations of actinomycetes, bacteria and fungi decreased.
publishDate 2006
dc.date.issued.fl_str_mv 2006-03-20
dc.date.available.fl_str_mv 2007-10-15
2015-03-26T13:37:56Z
dc.date.accessioned.fl_str_mv 2015-03-26T13:37:56Z
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dc.identifier.citation.fl_str_mv SCHURT, Daniel Augusto. Potential do allyl isothiocyanate to control Sclerotium rolfsii and Sclerotinia sclerotiorum. 2006. 57 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2006.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/4447
identifier_str_mv SCHURT, Daniel Augusto. Potential do allyl isothiocyanate to control Sclerotium rolfsii and Sclerotinia sclerotiorum. 2006. 57 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2006.
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