Identificação e caracterização de genes que codificam proteínas secretadas por Hemileia vastatrix na interação com o cafeeiro

Detalhes bibliográficos
Autor(a) principal: Fernandes, Michelle Bayerl
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: por
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://locus.ufv.br/handle/123456789/4390
Resumo: The coffee leaf rust caused by the biotrophic fungus Hemileia vastatrix, it is the most important fungal disease of coffee, causing productivity losses and its control increases the costs of coffee production. This study aimed to identify genes of H. vastatrix that encode secreted proteins that may function as effectors needed for the establishment of the biotrophic interaction and / or as triggers of resistance responses, by analyzing a database consisting of 9828 expressed sequence tags (ESTs) from an susceptible interaction. These ESTs were generated by sequencing the 5' end of cDNA clones from a library constructed from mRNA isolated from coffee leaves collected 12 days after inoculation with single pustule isolation HV-01. It was obtained 1004 contigs and 3301 singlets after clustering the sequences using the CAP3 program. From 890 unique transcripts that encode proteins without similarity to sequences of non-redundant database of GenBank / NCBI, which had no identity to sequences of Coffea spp., also deposited in this database, were identified 46 ORFs that encode peptides over 60 amino acids with positive prediction from five or more parameters of algorithms used to predict secretion signal sequences. We selected five genes which were amplified from the H. vastatrix genome, generating fragments equal or higher than those amplified from cDNAs. These genes, unique to H. vastatrix showed no identity with cDNA sequences derived from germination spores, demonstrating that their expression may occur within the infected tissue. The secretion of proteins encoded by four genes was demonstrated using the Yeast Secretion System. Functional studies should be conducted to confirm the effector activity of the genes characterized, as well as from other genes identified in this study, whose origin and secretion of fungal the predicted proteins in yeast has been demonstrated.
id UFV_bd6d4727a406f983c1909efa0c6e72cd
oai_identifier_str oai:locus.ufv.br:123456789/4390
network_acronym_str UFV
network_name_str LOCUS Repositório Institucional da UFV
repository_id_str 2145
spelling Fernandes, Michelle Bayerlhttp://lattes.cnpq.br/2277716495490007Pereira, Olinto Liparinihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767879D4Mizubuti, Eduardo Seiti Gomidehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785633J8Brommonschenkel, Sérgio Hermíniohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780948Y4Queiroz, Marisa Vieira dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785812Z5Furtado, Gleiber Quintãohttp://lattes.cnpq.br/16764925089494642015-03-26T13:37:44Z2011-11-012015-03-26T13:37:44Z2011-02-24FERNANDES, Michelle Bayerl. Identification and characterization of genes encoding proteins secreted by Hemileia vastatrix during interaction with coffee. 2011. 97 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2011.http://locus.ufv.br/handle/123456789/4390The coffee leaf rust caused by the biotrophic fungus Hemileia vastatrix, it is the most important fungal disease of coffee, causing productivity losses and its control increases the costs of coffee production. This study aimed to identify genes of H. vastatrix that encode secreted proteins that may function as effectors needed for the establishment of the biotrophic interaction and / or as triggers of resistance responses, by analyzing a database consisting of 9828 expressed sequence tags (ESTs) from an susceptible interaction. These ESTs were generated by sequencing the 5' end of cDNA clones from a library constructed from mRNA isolated from coffee leaves collected 12 days after inoculation with single pustule isolation HV-01. It was obtained 1004 contigs and 3301 singlets after clustering the sequences using the CAP3 program. From 890 unique transcripts that encode proteins without similarity to sequences of non-redundant database of GenBank / NCBI, which had no identity to sequences of Coffea spp., also deposited in this database, were identified 46 ORFs that encode peptides over 60 amino acids with positive prediction from five or more parameters of algorithms used to predict secretion signal sequences. We selected five genes which were amplified from the H. vastatrix genome, generating fragments equal or higher than those amplified from cDNAs. These genes, unique to H. vastatrix showed no identity with cDNA sequences derived from germination spores, demonstrating that their expression may occur within the infected tissue. The secretion of proteins encoded by four genes was demonstrated using the Yeast Secretion System. Functional studies should be conducted to confirm the effector activity of the genes characterized, as well as from other genes identified in this study, whose origin and secretion of fungal the predicted proteins in yeast has been demonstrated.A ferrugem causada pelo fungo biotrófico Hemileia vastatrix é a doença mais importante do cafeeiro, pois atinge, com gravidade, grandes áreas de lavouras, onde causa prejuízos na produtividade e seu controle aumenta os custos de produção. O presente trabalho teve por objetivo identificar genes de H. vastatrix que codificam proteínas secretadas que possam funcionar como efetores necessários para o estabelecimento da interação biotrófica e, ou, como desencadeadores de respostas de resistência, por meio da análise de um banco constituídos por 9828 etiquetas de sequências expressas (ESTs) em uma interação suscetível. Essas ESTs foram geradas pelo sequenciamento da extremidade 5´de clones de cDNA de uma biblioteca construída a partir de mRNA isolado de folhas de cafeeiro coletadas 12 dias após a inoculação com o isolado monopustular HV-01. Foram obtidos 1004 contíguos e 3301 singletos após o alinhamento das sequências pelo programa CAP3. A partir de 890 transcritos únicos que codificam proteínas sem similaridade a sequências do banco não redundante do GenBank/NCBI e que não possuíam identidade a sequências de Coffea spp. também depositadas nesse banco de dados, foram obtidas 46 ORFs que codificam peptídeos com mais de 60 aminoácidos e predição positiva em cinco ou mais parâmetros do algoritmo de predição de sequências sinal de exportação SignalP. Foram selecionados cinco genes que tiveram a região da ORF amplificada do genoma de H. vastatrix, gerando fragmentos iguais ou maiores aos amplificados a partir do cDNA. Esses genes, exclusivos de H. vastatrix, não mostraram identidade com sequências únicas derivadas de esporos germinados desse patógeno, demonstrando que a sua expressão ocorre no interior do tecido infectado. A secreção das proteínas codificadas por quatro genes foi confirmada em levedura. Estudos funcionais deverão ser realizados para comprovar a atividade efetora dos genes caracterizados, assim como dos demais genes identificados nesse estudo, cuja origem fúngica e secreção das proteínas preditas em levedura seja demonstrada.Fundação de Amparo a Pesquisa do Estado de Minas Geraisapplication/pdfporUniversidade Federal de ViçosaMestrado em FitopatologiaUFVBREtiologia; Epidemiologia; ControleFerrugem do cafeeiroHemileia vastatrixEfetoresCoffee rustHemileia vastatrixEffectorsCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIAIdentificação e caracterização de genes que codificam proteínas secretadas por Hemileia vastatrix na interação com o cafeeiroIdentification and characterization of genes encoding proteins secreted by Hemileia vastatrix during interaction with coffeeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf1333692https://locus.ufv.br//bitstream/123456789/4390/1/texto%20completo.pdf9bcd2dfa7f5cb679627a7e4240a82858MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain217345https://locus.ufv.br//bitstream/123456789/4390/2/texto%20completo.pdf.txt5318993569924fa6283fd1563e49d146MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3702https://locus.ufv.br//bitstream/123456789/4390/3/texto%20completo.pdf.jpgcbe35635ef095cdacf6c9458fd95d5e5MD53123456789/43902016-04-10 23:09:05.317oai:locus.ufv.br:123456789/4390Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-11T02:09:05LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Identificação e caracterização de genes que codificam proteínas secretadas por Hemileia vastatrix na interação com o cafeeiro
dc.title.alternative.eng.fl_str_mv Identification and characterization of genes encoding proteins secreted by Hemileia vastatrix during interaction with coffee
title Identificação e caracterização de genes que codificam proteínas secretadas por Hemileia vastatrix na interação com o cafeeiro
spellingShingle Identificação e caracterização de genes que codificam proteínas secretadas por Hemileia vastatrix na interação com o cafeeiro
Fernandes, Michelle Bayerl
Ferrugem do cafeeiro
Hemileia vastatrix
Efetores
Coffee rust
Hemileia vastatrix
Effectors
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
title_short Identificação e caracterização de genes que codificam proteínas secretadas por Hemileia vastatrix na interação com o cafeeiro
title_full Identificação e caracterização de genes que codificam proteínas secretadas por Hemileia vastatrix na interação com o cafeeiro
title_fullStr Identificação e caracterização de genes que codificam proteínas secretadas por Hemileia vastatrix na interação com o cafeeiro
title_full_unstemmed Identificação e caracterização de genes que codificam proteínas secretadas por Hemileia vastatrix na interação com o cafeeiro
title_sort Identificação e caracterização de genes que codificam proteínas secretadas por Hemileia vastatrix na interação com o cafeeiro
author Fernandes, Michelle Bayerl
author_facet Fernandes, Michelle Bayerl
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/2277716495490007
dc.contributor.author.fl_str_mv Fernandes, Michelle Bayerl
dc.contributor.advisor-co1.fl_str_mv Pereira, Olinto Liparini
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767879D4
dc.contributor.advisor-co2.fl_str_mv Mizubuti, Eduardo Seiti Gomide
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785633J8
dc.contributor.advisor1.fl_str_mv Brommonschenkel, Sérgio Hermínio
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780948Y4
dc.contributor.referee1.fl_str_mv Queiroz, Marisa Vieira de
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785812Z5
dc.contributor.referee2.fl_str_mv Furtado, Gleiber Quintão
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/1676492508949464
contributor_str_mv Pereira, Olinto Liparini
Mizubuti, Eduardo Seiti Gomide
Brommonschenkel, Sérgio Hermínio
Queiroz, Marisa Vieira de
Furtado, Gleiber Quintão
dc.subject.por.fl_str_mv Ferrugem do cafeeiro
Hemileia vastatrix
Efetores
topic Ferrugem do cafeeiro
Hemileia vastatrix
Efetores
Coffee rust
Hemileia vastatrix
Effectors
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
dc.subject.eng.fl_str_mv Coffee rust
Hemileia vastatrix
Effectors
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA
description The coffee leaf rust caused by the biotrophic fungus Hemileia vastatrix, it is the most important fungal disease of coffee, causing productivity losses and its control increases the costs of coffee production. This study aimed to identify genes of H. vastatrix that encode secreted proteins that may function as effectors needed for the establishment of the biotrophic interaction and / or as triggers of resistance responses, by analyzing a database consisting of 9828 expressed sequence tags (ESTs) from an susceptible interaction. These ESTs were generated by sequencing the 5' end of cDNA clones from a library constructed from mRNA isolated from coffee leaves collected 12 days after inoculation with single pustule isolation HV-01. It was obtained 1004 contigs and 3301 singlets after clustering the sequences using the CAP3 program. From 890 unique transcripts that encode proteins without similarity to sequences of non-redundant database of GenBank / NCBI, which had no identity to sequences of Coffea spp., also deposited in this database, were identified 46 ORFs that encode peptides over 60 amino acids with positive prediction from five or more parameters of algorithms used to predict secretion signal sequences. We selected five genes which were amplified from the H. vastatrix genome, generating fragments equal or higher than those amplified from cDNAs. These genes, unique to H. vastatrix showed no identity with cDNA sequences derived from germination spores, demonstrating that their expression may occur within the infected tissue. The secretion of proteins encoded by four genes was demonstrated using the Yeast Secretion System. Functional studies should be conducted to confirm the effector activity of the genes characterized, as well as from other genes identified in this study, whose origin and secretion of fungal the predicted proteins in yeast has been demonstrated.
publishDate 2011
dc.date.available.fl_str_mv 2011-11-01
2015-03-26T13:37:44Z
dc.date.issued.fl_str_mv 2011-02-24
dc.date.accessioned.fl_str_mv 2015-03-26T13:37:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERNANDES, Michelle Bayerl. Identification and characterization of genes encoding proteins secreted by Hemileia vastatrix during interaction with coffee. 2011. 97 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2011.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/4390
identifier_str_mv FERNANDES, Michelle Bayerl. Identification and characterization of genes encoding proteins secreted by Hemileia vastatrix during interaction with coffee. 2011. 97 f. Dissertação (Mestrado em Etiologia; Epidemiologia; Controle) - Universidade Federal de Viçosa, Viçosa, 2011.
url http://locus.ufv.br/handle/123456789/4390
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Viçosa
dc.publisher.program.fl_str_mv Mestrado em Fitopatologia
dc.publisher.initials.fl_str_mv UFV
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Etiologia; Epidemiologia; Controle
publisher.none.fl_str_mv Universidade Federal de Viçosa
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
bitstream.url.fl_str_mv https://locus.ufv.br//bitstream/123456789/4390/1/texto%20completo.pdf
https://locus.ufv.br//bitstream/123456789/4390/2/texto%20completo.pdf.txt
https://locus.ufv.br//bitstream/123456789/4390/3/texto%20completo.pdf.jpg
bitstream.checksum.fl_str_mv 9bcd2dfa7f5cb679627a7e4240a82858
5318993569924fa6283fd1563e49d146
cbe35635ef095cdacf6c9458fd95d5e5
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
_version_ 1801213008522772480