Diversidade genética de populações de Hemileia vastatrix no estado de Minas Gerais

• Autor(a) principal: Nunes, Cristiano Caixeta
• Data de Publicação: 2006
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/4448
• topic: Café; Ferrugem do cafeeiro; RAPD; Hemileia vastatrix; Coffee; Coffee leaf rust; RAPD; Hemileia vastatrix; CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOSSANIDADE::FITOPATOLOGIA

A total of 120 isolates of Hemileia vastatrix, obtained from two coffee crop systems (conventional and organic) and six counties from two main production regions in Minas Gerais (Zona da Mata and Sul de Minas), sampled and multiplied in 2005, were characterized by microsatellite and RAPD markers. After checking DNA integrity of the samples by ITS 1 and ITS 4 primer pairs, we assessed the genetic diversity of all isolates with three microsatellite primer pairs and three RAPD primers. Bands from microsatellite primers were monomorphic, whereas bands from RAPD primers were polymorphic. Over all loci, the genetic diversity (h) was 0.28 and the genetic differentiation (Gst) was 0.22, therefore the Gst was considered high. Higher h (0.29) and Gst (0.25) values were estimated for the crops under organic system. When regions were compared, for Zona da Mata we estimated high h (0.27), but low Gst (0.17) values. The highest h and number of migrants (Nm) values were estimated for Tombos (0.29) and Poços de Caldas (11.90) counties, respectively. The cluster analysis did not show any structure regarding to the origin, crop system or production region of the isolates.