Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1186/s12985-018-0930-x http://www.locus.ufv.br/handle/123456789/18626 |
Resumo: | Since the discovery of giant viruses infecting amoebae in 2003, many dogmas of virology have been revised and the search for these viruses has been intensified. Over the last few years, several new groups of these viruses have been discovered in various types of samples and environments.In this work, we describe the isolation of 68 giant viruses of amoeba obtained from environmental samples from Brazil and Antarctica. Isolated viruses were identified by hemacolor staining, PCR assays and electron microscopy (scanning and/or transmission). A total of 64 viruses belonging to the Mimiviridae family were isolated (26 from lineage A, 13 from lineage B, 2 from lineage C and 23 from unidentified lineages) from different types of samples, including marine water from Antarctica, thus being the first mimiviruses isolated in this extreme environment to date. Furthermore, a marseillevirus was isolated from sewage samples along with two pandoraviruses and a cedratvirus (the third to be isolated in the world so far). Considering the different type of samples, we found a higher number of viral groups in sewage samples. Our results reinforce the importance of prospective studies in different environmental samples, therefore improving our comprehension about the circulation anddiversity of these viruses in nature. |
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Abrahão, WalterAndrade, Ana Cláudia dos S. P.Arantes, Thalita S.Rodrigues, Rodrigo A. L.Machado, Talita B.Dornas, Fábio P.Landell, Melissa F.Furst, CinthiaBorges, Luiz G. A.Dutra, Lara A. L.Almeida, GabrielTrindade, Giliane de S.Bergier, IvanBorges, Iara A.Cortines, Juliana R.Oliveira, Danilo B. deKroon, Erna G.Abrahão, Jônatas S.2018-04-04T10:39:36Z2018-04-04T10:39:36Z2018-01-241743422Xhttp://dx.doi.org/10.1186/s12985-018-0930-xhttp://www.locus.ufv.br/handle/123456789/18626Since the discovery of giant viruses infecting amoebae in 2003, many dogmas of virology have been revised and the search for these viruses has been intensified. Over the last few years, several new groups of these viruses have been discovered in various types of samples and environments.In this work, we describe the isolation of 68 giant viruses of amoeba obtained from environmental samples from Brazil and Antarctica. Isolated viruses were identified by hemacolor staining, PCR assays and electron microscopy (scanning and/or transmission). A total of 64 viruses belonging to the Mimiviridae family were isolated (26 from lineage A, 13 from lineage B, 2 from lineage C and 23 from unidentified lineages) from different types of samples, including marine water from Antarctica, thus being the first mimiviruses isolated in this extreme environment to date. Furthermore, a marseillevirus was isolated from sewage samples along with two pandoraviruses and a cedratvirus (the third to be isolated in the world so far). Considering the different type of samples, we found a higher number of viral groups in sewage samples. Our results reinforce the importance of prospective studies in different environmental samples, therefore improving our comprehension about the circulation anddiversity of these viruses in nature.engVirology Journalv. 15, n. 22, p. 01-10, 2018Giant virusesProspectionBrazilAntarcticaPandoravirusCedratvirusMarseillevirusMimivirusUbiquitous giants: a plethora of giant viruses found in Brazil and Antarcticainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdftexto completoapplication/pdf4122848https://locus.ufv.br//bitstream/123456789/18626/1/artigo.pdf9e261fa47d3d7b2cf4b0b7fdf2830ee5MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/18626/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5183https://locus.ufv.br//bitstream/123456789/18626/3/artigo.pdf.jpg26c92a6d5aaeb01251946ec4bee75c63MD53123456789/186262018-04-04 23:00:52.42oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-04-05T02:00:52LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica |
title |
Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica |
spellingShingle |
Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica Abrahão, Walter Giant viruses Prospection Brazil Antarctica Pandoravirus Cedratvirus Marseillevirus Mimivirus |
title_short |
Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica |
title_full |
Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica |
title_fullStr |
Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica |
title_full_unstemmed |
Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica |
title_sort |
Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica |
author |
Abrahão, Walter |
author_facet |
Abrahão, Walter Andrade, Ana Cláudia dos S. P. Arantes, Thalita S. Rodrigues, Rodrigo A. L. Machado, Talita B. Dornas, Fábio P. Landell, Melissa F. Furst, Cinthia Borges, Luiz G. A. Dutra, Lara A. L. Almeida, Gabriel Trindade, Giliane de S. Bergier, Ivan Borges, Iara A. Cortines, Juliana R. Oliveira, Danilo B. de Kroon, Erna G. Abrahão, Jônatas S. |
author_role |
author |
author2 |
Andrade, Ana Cláudia dos S. P. Arantes, Thalita S. Rodrigues, Rodrigo A. L. Machado, Talita B. Dornas, Fábio P. Landell, Melissa F. Furst, Cinthia Borges, Luiz G. A. Dutra, Lara A. L. Almeida, Gabriel Trindade, Giliane de S. Bergier, Ivan Borges, Iara A. Cortines, Juliana R. Oliveira, Danilo B. de Kroon, Erna G. Abrahão, Jônatas S. |
author2_role |
author author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Abrahão, Walter Andrade, Ana Cláudia dos S. P. Arantes, Thalita S. Rodrigues, Rodrigo A. L. Machado, Talita B. Dornas, Fábio P. Landell, Melissa F. Furst, Cinthia Borges, Luiz G. A. Dutra, Lara A. L. Almeida, Gabriel Trindade, Giliane de S. Bergier, Ivan Borges, Iara A. Cortines, Juliana R. Oliveira, Danilo B. de Kroon, Erna G. Abrahão, Jônatas S. |
dc.subject.pt-BR.fl_str_mv |
Giant viruses Prospection Brazil Antarctica Pandoravirus Cedratvirus Marseillevirus Mimivirus |
topic |
Giant viruses Prospection Brazil Antarctica Pandoravirus Cedratvirus Marseillevirus Mimivirus |
description |
Since the discovery of giant viruses infecting amoebae in 2003, many dogmas of virology have been revised and the search for these viruses has been intensified. Over the last few years, several new groups of these viruses have been discovered in various types of samples and environments.In this work, we describe the isolation of 68 giant viruses of amoeba obtained from environmental samples from Brazil and Antarctica. Isolated viruses were identified by hemacolor staining, PCR assays and electron microscopy (scanning and/or transmission). A total of 64 viruses belonging to the Mimiviridae family were isolated (26 from lineage A, 13 from lineage B, 2 from lineage C and 23 from unidentified lineages) from different types of samples, including marine water from Antarctica, thus being the first mimiviruses isolated in this extreme environment to date. Furthermore, a marseillevirus was isolated from sewage samples along with two pandoraviruses and a cedratvirus (the third to be isolated in the world so far). Considering the different type of samples, we found a higher number of viral groups in sewage samples. Our results reinforce the importance of prospective studies in different environmental samples, therefore improving our comprehension about the circulation anddiversity of these viruses in nature. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-04-04T10:39:36Z |
dc.date.available.fl_str_mv |
2018-04-04T10:39:36Z |
dc.date.issued.fl_str_mv |
2018-01-24 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12985-018-0930-x http://www.locus.ufv.br/handle/123456789/18626 |
dc.identifier.issn.none.fl_str_mv |
1743422X |
identifier_str_mv |
1743422X |
url |
http://dx.doi.org/10.1186/s12985-018-0930-x http://www.locus.ufv.br/handle/123456789/18626 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
v. 15, n. 22, p. 01-10, 2018 |
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Virology Journal |
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Virology Journal |
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