Uso de L-Glutamina e Ácido Glutâmico + L-Glutamina em Rações para Leitões

• Autor(a) principal: Lescano, Diego Alberto
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: http://locus.ufv.br/handle/123456789/5819
• topic: Suíno - Alimentação e rações; Glutamina; Ácido glutâmico; Pig - Food and feed; Glutamine; Glutamic acid; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMAL::AVALIACAO DE ALIMENTOS PARA ANIMAIS

Four experiments were conducted to evaluate the effect of the use of four levels of L-glutamine (Gln) and four levels of Glutamic acid + L-glutamine in the diet of piglets after weaning, two performance tests and two tests of intestinal morphohistology. In each test diets were formulated based on corn, soybean meal, cooked corn, dairy products, blood plasma and industrial amino acids. In the performance trial (trial 1) forty-four (44) piglets were distributed in four treatments (T1 - 0.0%; T2 - 0.4%; T3 - 0.8% and T4 - 1.2% of Gln) on five replications, on 4 repetitions with 2 animals per experimental unit and one repetition with 3 animals per experimental unit. The experimental period was of 28 days, with the first period 18-32 days of life, the second instead of 32-46 days of life and overall period 18-46 days. The evaluated parameters were average daily feed intake (ADFI), average daily weight gain (ADG) and feed conversion (F:G). The addition of Gln in the diet did not affect (P> 0.05) performance parameters in periods. In the testing of morphohistology (trial 2) twenty-four (24) piglets of 18 days old were divided into four treatments (T1 - 0.0%, T2 - 0.4%, T3 - 0.8% and T4- 1, 2% of Gln) with six replicates and one animal per experimental unit. The animals were slaughtered at 7 days after weaning (25 days old) to collect samples of the three intestinal portions. The histologic evaluated parameters were villus height, crypt depth and villus height: crypt depth ratio of three segments. The addition of Gln in feed promoted linear and linear-quadratic reduction (P <0.05) in the villus height and crypt depth of the duodenum of piglets respectively. It is concluded that the addition of Gln in diets of piglets after weaning showed no beneficial effects on performance and in maintaining the integrity of the intestinal mucosa of piglets. In the performance test (Test 3) forty-four (44) piglets were distributed among four treatments (T1- 0.0%; T2 - 0.4%; T3-0.8% and T4 - 1.2% of Glutamic acid + Gln) on five replications, on 4 repetitions on 2 animals per experimental unit and one repetition with 3 animals per experimental unit. The experimental period was of 28 days, with the first period 18-32 days of life, the second instead of 32-46 days of life and total period 18-46 days. The evaluated parameters were daily feed intake (ADFI), daily weight gain (ADG) and feed conversion (F:G). Addition of Glutamic Acid + Gln in diets of piglets after weaning improved linearly (P <0.05) ADFI, ADG and F:G in the period 18-32 days of age, however significant effects were not observed (P> 0 , 05) the second instead of 32- 46 days old, since the total period showed a linear improvement (P <0.05) in the ADFI and F:G, and linear-quadratic improvement in ADG. In the testing of morphohistology (test 4) twenty-four (24) piglets of 18 days of age were divided into four treatments (T1 - 0.0%, T2 - 0.4%, T3 0,8% and T4-1 2% of glutamic Acid + Gln) with six replicates and one animal per experimental unit. The animals were slaughtered 7 days after weaning (25 days old) to collect samples of the three intestinal portions. The histologic parameters evaluated were villus height, crypt depth and villus height: crypt depth ratio of three segments. The addition of Glutamic Acid + Gln in the diet of piglets after weaning showed a linear effect (P <0.05) for the parameters of villus height and villus height: crypt depth ratio of duodenal villus height: crypt depth ratio of jejunum, villus height and villus height: crypt depth ratio of the ileum. As for the crypt depth duodenal parameter observed linear-quadratic effect. It is concluded that the addition of Glutamic Acid + Gln in diets of piglets after weaning showed beneficial effects on performance and in maintaining the integrity of the intestinal mucosa of piglets.