The role of minocycline in cognitive impairment and dysfunction of the blood brain barrier in experimental pneumococcal meningitis

Detalhes bibliográficos
Autor(a) principal: Aveline, Paulo Eduardo
Data de Publicação: 2015
Outros Autores: Generoso, Jaqueline S, Simões, Lutiana R, Sangiogo, Gustavo, Valvassori, Samira S, Quevedo, João
Idioma: eng
Título da fonte: Repositório Institucional da UNESC
Texto Completo: http://repositorio.unesc.net/handle/1/7390
Resumo: Artigo submetido ao Curso de Medicina da UNESC como requisito parcial para obtenção do Título de Bacharel em Medicina.
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spelling Aveline, Paulo EduardoGeneroso, Jaqueline SSimões, Lutiana RSangiogo, GustavoValvassori, Samira SQuevedo, JoãoBarichello, TatianaUniversidade do Extremo Sul Catarinense2019-12-10T15:03:07Z2019-12-10T15:03:07Z2015-12http://repositorio.unesc.net/handle/1/7390Artigo submetido ao Curso de Medicina da UNESC como requisito parcial para obtenção do Título de Bacharel em Medicina.Bacterial meningitis is a life threatening infection associated with cognitive impairment in many survivors. The pathogen invades the CNS by penetrating through the luminal side of the cerebral endothelium, which is an integral part of the BBB. Microglia are the resident macrophages of the CNS which can trigger a host of immunological pathways. The inflammatory response from microglial activation can facilitate the elimination of invasive microorganisms; however, excessive or extended microglial activation can result in neuronal damage and eventually cell death. The inhibition of microglia using minocycline can be a relevant pharmacological tool to study the role of microglia in different CNS diseases. In this study, animals received either artificial cerebrospinal fluid or a Streptococcus pneumoniae suspension. The animals receive minocycline or saline immediately after induction. For the evaluation of the BBB integrity, the animals were killed at 12, 18 and 24 h after induction. For the behavioural tests, ten days after meningitis was induced, were subjected to open-field habituation and the step-down inhibitory task. In both cerebral structures the use of the minocycline prevented BBB disruption. In the behavioural tests the use of minocycline prevented habituation and aversive memory impairment in the meningitis/minocycline group when compared with meningitis/saline. Our results demonstrate that the minocycline was able to decrease long-term cognitive impairment and BBB dysfunction in rats survivors of meningitis representing a new pharmacological approach towards pneumococcal meningitis.Bacterial meningitisMinocyclineMicrogliaCognitive impairmentBlood-brain barrierThe role of minocycline in cognitive impairment and dysfunction of the blood brain barrier in experimental pneumococcal meningitisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisengreponame:Repositório Institucional da UNESCinstname:Universidade do Extremo Sul Catarinense (Unesc)instacron:UNESCinfo:eu-repo/semantics/openAccessORIGINALPaulo Eduardo Damasio.pdfPaulo Eduardo Damasio.pdfapplication/pdf224970http://repositorio.unesc.net/bitstream/1/7390/1/Paulo%20Eduardo%20Damasio.pdf3ccceba1e98a97733518be362a470135MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.unesc.net/bitstream/1/7390/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD521/73902019-12-10 13:03:11.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Repositório de Publicaçõeshttp://repositorio.unesc.net/
dc.title.pt_BR.fl_str_mv The role of minocycline in cognitive impairment and dysfunction of the blood brain barrier in experimental pneumococcal meningitis
title The role of minocycline in cognitive impairment and dysfunction of the blood brain barrier in experimental pneumococcal meningitis
spellingShingle The role of minocycline in cognitive impairment and dysfunction of the blood brain barrier in experimental pneumococcal meningitis
Aveline, Paulo Eduardo
Bacterial meningitis
Minocycline
Microglia
Cognitive impairment
Blood-brain barrier
title_short The role of minocycline in cognitive impairment and dysfunction of the blood brain barrier in experimental pneumococcal meningitis
title_full The role of minocycline in cognitive impairment and dysfunction of the blood brain barrier in experimental pneumococcal meningitis
title_fullStr The role of minocycline in cognitive impairment and dysfunction of the blood brain barrier in experimental pneumococcal meningitis
title_full_unstemmed The role of minocycline in cognitive impairment and dysfunction of the blood brain barrier in experimental pneumococcal meningitis
title_sort The role of minocycline in cognitive impairment and dysfunction of the blood brain barrier in experimental pneumococcal meningitis
author Aveline, Paulo Eduardo
author_facet Aveline, Paulo Eduardo
Generoso, Jaqueline S
Simões, Lutiana R
Sangiogo, Gustavo
Valvassori, Samira S
Quevedo, João
author_role author
author2 Generoso, Jaqueline S
Simões, Lutiana R
Sangiogo, Gustavo
Valvassori, Samira S
Quevedo, João
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Aveline, Paulo Eduardo
Generoso, Jaqueline S
Simões, Lutiana R
Sangiogo, Gustavo
Valvassori, Samira S
Quevedo, João
dc.contributor.advisor1.fl_str_mv Barichello, Tatiana
contributor_str_mv Barichello, Tatiana
dc.subject.por.fl_str_mv Bacterial meningitis
Minocycline
Microglia
Cognitive impairment
Blood-brain barrier
topic Bacterial meningitis
Minocycline
Microglia
Cognitive impairment
Blood-brain barrier
dc.description.pt_BR.fl_txt_mv Artigo submetido ao Curso de Medicina da UNESC como requisito parcial para obtenção do Título de Bacharel em Medicina.
dc.description.abstract.por.fl_txt_mv Bacterial meningitis is a life threatening infection associated with cognitive impairment in many survivors. The pathogen invades the CNS by penetrating through the luminal side of the cerebral endothelium, which is an integral part of the BBB. Microglia are the resident macrophages of the CNS which can trigger a host of immunological pathways. The inflammatory response from microglial activation can facilitate the elimination of invasive microorganisms; however, excessive or extended microglial activation can result in neuronal damage and eventually cell death. The inhibition of microglia using minocycline can be a relevant pharmacological tool to study the role of microglia in different CNS diseases. In this study, animals received either artificial cerebrospinal fluid or a Streptococcus pneumoniae suspension. The animals receive minocycline or saline immediately after induction. For the evaluation of the BBB integrity, the animals were killed at 12, 18 and 24 h after induction. For the behavioural tests, ten days after meningitis was induced, were subjected to open-field habituation and the step-down inhibitory task. In both cerebral structures the use of the minocycline prevented BBB disruption. In the behavioural tests the use of minocycline prevented habituation and aversive memory impairment in the meningitis/minocycline group when compared with meningitis/saline. Our results demonstrate that the minocycline was able to decrease long-term cognitive impairment and BBB dysfunction in rats survivors of meningitis representing a new pharmacological approach towards pneumococcal meningitis.
description Artigo submetido ao Curso de Medicina da UNESC como requisito parcial para obtenção do Título de Bacharel em Medicina.
publishDate 2015
dc.date.created.fl_str_mv 2015-12
dc.date.accessioned.fl_str_mv 2019-12-10T15:03:07Z
dc.date.available.fl_str_mv 2019-12-10T15:03:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bachelorThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.unesc.net/handle/1/7390
url http://repositorio.unesc.net/handle/1/7390
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.coverage.spatial.pt_BR.fl_str_mv Universidade do Extremo Sul Catarinense
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNESC
instname:Universidade do Extremo Sul Catarinense (Unesc)
instacron:UNESC
instname_str Universidade do Extremo Sul Catarinense (Unesc)
instacron_str UNESC
institution UNESC
reponame_str Repositório Institucional da UNESC
collection Repositório Institucional da UNESC
bitstream.url.fl_str_mv http://repositorio.unesc.net/bitstream/1/7390/1/Paulo%20Eduardo%20Damasio.pdf
http://repositorio.unesc.net/bitstream/1/7390/2/license.txt
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