Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | The Journal of venomous animals and toxins including tropical diseases (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400013 |
Resumo: | Snake venom (sv) C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD). A very well studied sv C-type lectin is the heterodimeric toxin, convulxin (CVX), from the venoms of South American rattlesnakes, Crotalus durissus terrificus and C. d. cascavella. It consists of two subunits, alfa (CVXalpha , 13.9 kDa) and beta (CVXbeta , 12.6 kDa), joined by inter and intra-chain disulfide bounds, and is arranged in a tetrameric alpha4beta4 conformation. Convulxin is able to activate platelet and induce their aggregation by acting via p62/GPVI collagen receptor. Several cDNA precursors, homolog of CVX subunits, were cloned by PCR homology screening. As determined by computational analysis, one of them, named crotacetin beta subunit, was predicted as a polypeptide with a tridimensional conformation very similar to other subunits of convulxin-like snake toxins. Crotacetin was purified from C. durissus venoms by gel permeation and reverse phase high performance liquid chromatography. The heterodimeric crotacetin is expressed in the venoms of several C. durissus subspecies, but it is prevalent in the venom of C. durissus cascavella. As inferred from homology modeling, crotacetin induces platelet aggregation but noticeably exhibits antimicrobial activity against Gram-positive and Gram-negative bacteria. |
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The Journal of venomous animals and toxins including tropical diseases (Online) |
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Crotacetin, a novel snake venom C-type lectin, is homolog of convulxinCrotalus durisssus venomsnake venom C-type lectinhomology modelingplatelet aggregationantimicrobial activitySnake venom (sv) C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD). A very well studied sv C-type lectin is the heterodimeric toxin, convulxin (CVX), from the venoms of South American rattlesnakes, Crotalus durissus terrificus and C. d. cascavella. It consists of two subunits, alfa (CVXalpha , 13.9 kDa) and beta (CVXbeta , 12.6 kDa), joined by inter and intra-chain disulfide bounds, and is arranged in a tetrameric alpha4beta4 conformation. Convulxin is able to activate platelet and induce their aggregation by acting via p62/GPVI collagen receptor. Several cDNA precursors, homolog of CVX subunits, were cloned by PCR homology screening. As determined by computational analysis, one of them, named crotacetin beta subunit, was predicted as a polypeptide with a tridimensional conformation very similar to other subunits of convulxin-like snake toxins. Crotacetin was purified from C. durissus venoms by gel permeation and reverse phase high performance liquid chromatography. The heterodimeric crotacetin is expressed in the venoms of several C. durissus subspecies, but it is prevalent in the venom of C. durissus cascavella. As inferred from homology modeling, crotacetin induces platelet aggregation but noticeably exhibits antimicrobial activity against Gram-positive and Gram-negative bacteria.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2005-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400013Journal of Venomous Animals and Toxins including Tropical Diseases v.11 n.4 2005reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/S1678-91992005000400013info:eu-repo/semantics/openAccessRádis-Baptista,G.Moreno,F. B. M. B.Nogueira,L. L.Martins,A. M. C.Toyama,D. O.Toyama,M. H.Azevedo Jr,W. F.Cavada,B. S.Yamane,T.eng2005-11-21T00:00:00Zoai:scielo:S1678-91992005000400013Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2005-11-21T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
title |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
spellingShingle |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin Rádis-Baptista,G. Crotalus durisssus venom snake venom C-type lectin homology modeling platelet aggregation antimicrobial activity |
title_short |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
title_full |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
title_fullStr |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
title_full_unstemmed |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
title_sort |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
author |
Rádis-Baptista,G. |
author_facet |
Rádis-Baptista,G. Moreno,F. B. M. B. Nogueira,L. L. Martins,A. M. C. Toyama,D. O. Toyama,M. H. Azevedo Jr,W. F. Cavada,B. S. Yamane,T. |
author_role |
author |
author2 |
Moreno,F. B. M. B. Nogueira,L. L. Martins,A. M. C. Toyama,D. O. Toyama,M. H. Azevedo Jr,W. F. Cavada,B. S. Yamane,T. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rádis-Baptista,G. Moreno,F. B. M. B. Nogueira,L. L. Martins,A. M. C. Toyama,D. O. Toyama,M. H. Azevedo Jr,W. F. Cavada,B. S. Yamane,T. |
dc.subject.por.fl_str_mv |
Crotalus durisssus venom snake venom C-type lectin homology modeling platelet aggregation antimicrobial activity |
topic |
Crotalus durisssus venom snake venom C-type lectin homology modeling platelet aggregation antimicrobial activity |
description |
Snake venom (sv) C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD). A very well studied sv C-type lectin is the heterodimeric toxin, convulxin (CVX), from the venoms of South American rattlesnakes, Crotalus durissus terrificus and C. d. cascavella. It consists of two subunits, alfa (CVXalpha , 13.9 kDa) and beta (CVXbeta , 12.6 kDa), joined by inter and intra-chain disulfide bounds, and is arranged in a tetrameric alpha4beta4 conformation. Convulxin is able to activate platelet and induce their aggregation by acting via p62/GPVI collagen receptor. Several cDNA precursors, homolog of CVX subunits, were cloned by PCR homology screening. As determined by computational analysis, one of them, named crotacetin beta subunit, was predicted as a polypeptide with a tridimensional conformation very similar to other subunits of convulxin-like snake toxins. Crotacetin was purified from C. durissus venoms by gel permeation and reverse phase high performance liquid chromatography. The heterodimeric crotacetin is expressed in the venoms of several C. durissus subspecies, but it is prevalent in the venom of C. durissus cascavella. As inferred from homology modeling, crotacetin induces platelet aggregation but noticeably exhibits antimicrobial activity against Gram-positive and Gram-negative bacteria. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1678-91992005000400013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
dc.source.none.fl_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases v.11 n.4 2005 reponame:The Journal of venomous animals and toxins including tropical diseases (Online) instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
The Journal of venomous animals and toxins including tropical diseases (Online) |
collection |
The Journal of venomous animals and toxins including tropical diseases (Online) |
repository.name.fl_str_mv |
The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
||editorial@jvat.org.br |
_version_ |
1748958537581592576 |