Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin

Detalhes bibliográficos
Autor(a) principal: Rádis-baptista, G.
Data de Publicação: 2005
Outros Autores: Moreno, F. B. M. B., Nogueira, L. L., Martins, A. M. C., Toyama, D. O., Toyama, M. H. [UNESP], Azevedo Jr, W. F. [UNESP], Cavada, B. S., Yamane, T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1678-91992005000400013
http://hdl.handle.net/11449/213102
Resumo: Snake venom (sv) C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD). A very well studied sv C-type lectin is the heterodimeric toxin, convulxin (CVX), from the venoms of South American rattlesnakes, Crotalus durissus terrificus and C. d. cascavella. It consists of two subunits, alfa (CVXalpha , 13.9 kDa) and beta (CVXbeta , 12.6 kDa), joined by inter and intra-chain disulfide bounds, and is arranged in a tetrameric alpha4beta4 conformation. Convulxin is able to activate platelet and induce their aggregation by acting via p62/GPVI collagen receptor. Several cDNA precursors, homolog of CVX subunits, were cloned by PCR homology screening. As determined by computational analysis, one of them, named crotacetin beta subunit, was predicted as a polypeptide with a tridimensional conformation very similar to other subunits of convulxin-like snake toxins. Crotacetin was purified from C. durissus venoms by gel permeation and reverse phase high performance liquid chromatography. The heterodimeric crotacetin is expressed in the venoms of several C. durissus subspecies, but it is prevalent in the venom of C. durissus cascavella. As inferred from homology modeling, crotacetin induces platelet aggregation but noticeably exhibits antimicrobial activity against Gram-positive and Gram-negative bacteria.
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spelling Crotacetin, a novel snake venom C-type lectin, is homolog of convulxinCrotalus durisssus venomsnake venom C-type lectinhomology modelingplatelet aggregationantimicrobial activitySnake venom (sv) C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD). A very well studied sv C-type lectin is the heterodimeric toxin, convulxin (CVX), from the venoms of South American rattlesnakes, Crotalus durissus terrificus and C. d. cascavella. It consists of two subunits, alfa (CVXalpha , 13.9 kDa) and beta (CVXbeta , 12.6 kDa), joined by inter and intra-chain disulfide bounds, and is arranged in a tetrameric alpha4beta4 conformation. Convulxin is able to activate platelet and induce their aggregation by acting via p62/GPVI collagen receptor. Several cDNA precursors, homolog of CVX subunits, were cloned by PCR homology screening. As determined by computational analysis, one of them, named crotacetin beta subunit, was predicted as a polypeptide with a tridimensional conformation very similar to other subunits of convulxin-like snake toxins. Crotacetin was purified from C. durissus venoms by gel permeation and reverse phase high performance liquid chromatography. The heterodimeric crotacetin is expressed in the venoms of several C. durissus subspecies, but it is prevalent in the venom of C. durissus cascavella. As inferred from homology modeling, crotacetin induces platelet aggregation but noticeably exhibits antimicrobial activity against Gram-positive and Gram-negative bacteria.Federal University of Ceará, Department of Biochemistry and Molecular BiologyUniversidade Estadual Paulista, Humanities and Exact SciencesUniversidade Estadual de Campinas, Department of BiochemistryUniversidade Estadual Paulista, Department of ChemistryInstitute of Nuclear Energy and Research, Molecular Biology CenterFederal University of Ceará, Department of Clinical and Toxicological AnalysesUniversidade Estadual Paulista, Humanities and Exact SciencesUniversidade Estadual Paulista, Department of ChemistryCentro de Estudos de Venenos e Animais PeçonhentosFederal University of CearáUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Institute of Nuclear Energy and ResearchRádis-baptista, G.Moreno, F. B. M. B.Nogueira, L. L.Martins, A. M. C.Toyama, D. O.Toyama, M. H. [UNESP]Azevedo Jr, W. F. [UNESP]Cavada, B. S.Yamane, T.2021-07-14T10:50:00Z2021-07-14T10:50:00Z2005-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article557-578application/pdfhttp://dx.doi.org/10.1590/S1678-91992005000400013Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 4, p. 557-578, 2005.1678-9199http://hdl.handle.net/11449/21310210.1590/S1678-91992005000400013S1678-91992005000400013S1678-91992005000400013.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins including Tropical Diseasesinfo:eu-repo/semantics/openAccess2023-11-01T06:09:18Zoai:repositorio.unesp.br:11449/213102Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:35:32.455780Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin
title Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin
spellingShingle Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin
Rádis-baptista, G.
Crotalus durisssus venom
snake venom C-type lectin
homology modeling
platelet aggregation
antimicrobial activity
title_short Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin
title_full Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin
title_fullStr Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin
title_full_unstemmed Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin
title_sort Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin
author Rádis-baptista, G.
author_facet Rádis-baptista, G.
Moreno, F. B. M. B.
Nogueira, L. L.
Martins, A. M. C.
Toyama, D. O.
Toyama, M. H. [UNESP]
Azevedo Jr, W. F. [UNESP]
Cavada, B. S.
Yamane, T.
author_role author
author2 Moreno, F. B. M. B.
Nogueira, L. L.
Martins, A. M. C.
Toyama, D. O.
Toyama, M. H. [UNESP]
Azevedo Jr, W. F. [UNESP]
Cavada, B. S.
Yamane, T.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Federal University of Ceará
Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
Institute of Nuclear Energy and Research
dc.contributor.author.fl_str_mv Rádis-baptista, G.
Moreno, F. B. M. B.
Nogueira, L. L.
Martins, A. M. C.
Toyama, D. O.
Toyama, M. H. [UNESP]
Azevedo Jr, W. F. [UNESP]
Cavada, B. S.
Yamane, T.
dc.subject.por.fl_str_mv Crotalus durisssus venom
snake venom C-type lectin
homology modeling
platelet aggregation
antimicrobial activity
topic Crotalus durisssus venom
snake venom C-type lectin
homology modeling
platelet aggregation
antimicrobial activity
description Snake venom (sv) C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD). A very well studied sv C-type lectin is the heterodimeric toxin, convulxin (CVX), from the venoms of South American rattlesnakes, Crotalus durissus terrificus and C. d. cascavella. It consists of two subunits, alfa (CVXalpha , 13.9 kDa) and beta (CVXbeta , 12.6 kDa), joined by inter and intra-chain disulfide bounds, and is arranged in a tetrameric alpha4beta4 conformation. Convulxin is able to activate platelet and induce their aggregation by acting via p62/GPVI collagen receptor. Several cDNA precursors, homolog of CVX subunits, were cloned by PCR homology screening. As determined by computational analysis, one of them, named crotacetin beta subunit, was predicted as a polypeptide with a tridimensional conformation very similar to other subunits of convulxin-like snake toxins. Crotacetin was purified from C. durissus venoms by gel permeation and reverse phase high performance liquid chromatography. The heterodimeric crotacetin is expressed in the venoms of several C. durissus subspecies, but it is prevalent in the venom of C. durissus cascavella. As inferred from homology modeling, crotacetin induces platelet aggregation but noticeably exhibits antimicrobial activity against Gram-positive and Gram-negative bacteria.
publishDate 2005
dc.date.none.fl_str_mv 2005-12
2021-07-14T10:50:00Z
2021-07-14T10:50:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-91992005000400013
Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 4, p. 557-578, 2005.
1678-9199
http://hdl.handle.net/11449/213102
10.1590/S1678-91992005000400013
S1678-91992005000400013
S1678-91992005000400013.pdf
url http://dx.doi.org/10.1590/S1678-91992005000400013
http://hdl.handle.net/11449/213102
identifier_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 4, p. 557-578, 2005.
1678-9199
10.1590/S1678-91992005000400013
S1678-91992005000400013
S1678-91992005000400013.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 557-578
application/pdf
dc.publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos
publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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