Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin
Autor(a) principal: | |
---|---|
Data de Publicação: | 2005 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-91992005000400013 http://hdl.handle.net/11449/213102 |
Resumo: | Snake venom (sv) C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD). A very well studied sv C-type lectin is the heterodimeric toxin, convulxin (CVX), from the venoms of South American rattlesnakes, Crotalus durissus terrificus and C. d. cascavella. It consists of two subunits, alfa (CVXalpha , 13.9 kDa) and beta (CVXbeta , 12.6 kDa), joined by inter and intra-chain disulfide bounds, and is arranged in a tetrameric alpha4beta4 conformation. Convulxin is able to activate platelet and induce their aggregation by acting via p62/GPVI collagen receptor. Several cDNA precursors, homolog of CVX subunits, were cloned by PCR homology screening. As determined by computational analysis, one of them, named crotacetin beta subunit, was predicted as a polypeptide with a tridimensional conformation very similar to other subunits of convulxin-like snake toxins. Crotacetin was purified from C. durissus venoms by gel permeation and reverse phase high performance liquid chromatography. The heterodimeric crotacetin is expressed in the venoms of several C. durissus subspecies, but it is prevalent in the venom of C. durissus cascavella. As inferred from homology modeling, crotacetin induces platelet aggregation but noticeably exhibits antimicrobial activity against Gram-positive and Gram-negative bacteria. |
id |
UNSP_91f531c8c6f2702c7b2b5368669181b5 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/213102 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxinCrotalus durisssus venomsnake venom C-type lectinhomology modelingplatelet aggregationantimicrobial activitySnake venom (sv) C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD). A very well studied sv C-type lectin is the heterodimeric toxin, convulxin (CVX), from the venoms of South American rattlesnakes, Crotalus durissus terrificus and C. d. cascavella. It consists of two subunits, alfa (CVXalpha , 13.9 kDa) and beta (CVXbeta , 12.6 kDa), joined by inter and intra-chain disulfide bounds, and is arranged in a tetrameric alpha4beta4 conformation. Convulxin is able to activate platelet and induce their aggregation by acting via p62/GPVI collagen receptor. Several cDNA precursors, homolog of CVX subunits, were cloned by PCR homology screening. As determined by computational analysis, one of them, named crotacetin beta subunit, was predicted as a polypeptide with a tridimensional conformation very similar to other subunits of convulxin-like snake toxins. Crotacetin was purified from C. durissus venoms by gel permeation and reverse phase high performance liquid chromatography. The heterodimeric crotacetin is expressed in the venoms of several C. durissus subspecies, but it is prevalent in the venom of C. durissus cascavella. As inferred from homology modeling, crotacetin induces platelet aggregation but noticeably exhibits antimicrobial activity against Gram-positive and Gram-negative bacteria.Federal University of Ceará, Department of Biochemistry and Molecular BiologyUniversidade Estadual Paulista, Humanities and Exact SciencesUniversidade Estadual de Campinas, Department of BiochemistryUniversidade Estadual Paulista, Department of ChemistryInstitute of Nuclear Energy and Research, Molecular Biology CenterFederal University of Ceará, Department of Clinical and Toxicological AnalysesUniversidade Estadual Paulista, Humanities and Exact SciencesUniversidade Estadual Paulista, Department of ChemistryCentro de Estudos de Venenos e Animais PeçonhentosFederal University of CearáUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Institute of Nuclear Energy and ResearchRádis-baptista, G.Moreno, F. B. M. B.Nogueira, L. L.Martins, A. M. C.Toyama, D. O.Toyama, M. H. [UNESP]Azevedo Jr, W. F. [UNESP]Cavada, B. S.Yamane, T.2021-07-14T10:50:00Z2021-07-14T10:50:00Z2005-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article557-578application/pdfhttp://dx.doi.org/10.1590/S1678-91992005000400013Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 4, p. 557-578, 2005.1678-9199http://hdl.handle.net/11449/21310210.1590/S1678-91992005000400013S1678-91992005000400013S1678-91992005000400013.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins including Tropical Diseasesinfo:eu-repo/semantics/openAccess2023-11-01T06:09:18Zoai:repositorio.unesp.br:11449/213102Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:35:32.455780Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
title |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
spellingShingle |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin Rádis-baptista, G. Crotalus durisssus venom snake venom C-type lectin homology modeling platelet aggregation antimicrobial activity |
title_short |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
title_full |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
title_fullStr |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
title_full_unstemmed |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
title_sort |
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin |
author |
Rádis-baptista, G. |
author_facet |
Rádis-baptista, G. Moreno, F. B. M. B. Nogueira, L. L. Martins, A. M. C. Toyama, D. O. Toyama, M. H. [UNESP] Azevedo Jr, W. F. [UNESP] Cavada, B. S. Yamane, T. |
author_role |
author |
author2 |
Moreno, F. B. M. B. Nogueira, L. L. Martins, A. M. C. Toyama, D. O. Toyama, M. H. [UNESP] Azevedo Jr, W. F. [UNESP] Cavada, B. S. Yamane, T. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Ceará Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Institute of Nuclear Energy and Research |
dc.contributor.author.fl_str_mv |
Rádis-baptista, G. Moreno, F. B. M. B. Nogueira, L. L. Martins, A. M. C. Toyama, D. O. Toyama, M. H. [UNESP] Azevedo Jr, W. F. [UNESP] Cavada, B. S. Yamane, T. |
dc.subject.por.fl_str_mv |
Crotalus durisssus venom snake venom C-type lectin homology modeling platelet aggregation antimicrobial activity |
topic |
Crotalus durisssus venom snake venom C-type lectin homology modeling platelet aggregation antimicrobial activity |
description |
Snake venom (sv) C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD). A very well studied sv C-type lectin is the heterodimeric toxin, convulxin (CVX), from the venoms of South American rattlesnakes, Crotalus durissus terrificus and C. d. cascavella. It consists of two subunits, alfa (CVXalpha , 13.9 kDa) and beta (CVXbeta , 12.6 kDa), joined by inter and intra-chain disulfide bounds, and is arranged in a tetrameric alpha4beta4 conformation. Convulxin is able to activate platelet and induce their aggregation by acting via p62/GPVI collagen receptor. Several cDNA precursors, homolog of CVX subunits, were cloned by PCR homology screening. As determined by computational analysis, one of them, named crotacetin beta subunit, was predicted as a polypeptide with a tridimensional conformation very similar to other subunits of convulxin-like snake toxins. Crotacetin was purified from C. durissus venoms by gel permeation and reverse phase high performance liquid chromatography. The heterodimeric crotacetin is expressed in the venoms of several C. durissus subspecies, but it is prevalent in the venom of C. durissus cascavella. As inferred from homology modeling, crotacetin induces platelet aggregation but noticeably exhibits antimicrobial activity against Gram-positive and Gram-negative bacteria. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-12 2021-07-14T10:50:00Z 2021-07-14T10:50:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-91992005000400013 Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 4, p. 557-578, 2005. 1678-9199 http://hdl.handle.net/11449/213102 10.1590/S1678-91992005000400013 S1678-91992005000400013 S1678-91992005000400013.pdf |
url |
http://dx.doi.org/10.1590/S1678-91992005000400013 http://hdl.handle.net/11449/213102 |
identifier_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 4, p. 557-578, 2005. 1678-9199 10.1590/S1678-91992005000400013 S1678-91992005000400013 S1678-91992005000400013.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
557-578 application/pdf |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128223570034688 |