Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study

Detalhes bibliográficos
Autor(a) principal: SOARES,Priscilla Barbosa Ferreira
Data de Publicação: 2016
Outros Autores: ROCHA,Aletheia Moraes, REIS,Manuella Verdinelli de Paula, MOURA,Camilla Christian Gomes, SOARES,Carlos José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista de Odontologia da UNESP
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-25772016000400234
Resumo: Abstract Objective This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO3), (3) Triethanolamine (C6H15NO3), (4) 2-Amino-2-Methil-1-Propanol (C4H11NO). Fibroblasts were plated at 2×104/ well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT formazan method. Data were analyzed by 3-way ANOVA followed by Tukey’s and Dunnet’s test. Result There are no significant effect on the cell viability regarding type of CW, period of evaluation, and the interactions between CW and period of evaluation, CW and pH adjustment method, pH adjustment method and period of evaluation (p>0.05). Conclusion The product used for CW pH adjustment did not influenced HFF viability, thought there are a tendency of better performance in natural CW.
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spelling Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro studyTooth avulsioncoconut watercell viabilityAbstract Objective This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO3), (3) Triethanolamine (C6H15NO3), (4) 2-Amino-2-Methil-1-Propanol (C4H11NO). Fibroblasts were plated at 2×104/ well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT formazan method. Data were analyzed by 3-way ANOVA followed by Tukey’s and Dunnet’s test. Result There are no significant effect on the cell viability regarding type of CW, period of evaluation, and the interactions between CW and period of evaluation, CW and pH adjustment method, pH adjustment method and period of evaluation (p>0.05). Conclusion The product used for CW pH adjustment did not influenced HFF viability, thought there are a tendency of better performance in natural CW.Universidade Estadual Paulista Júlio de Mesquita Filho2016-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-25772016000400234Revista de Odontologia da UNESP v.45 n.4 2016reponame:Revista de Odontologia da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/1807-2577.09216info:eu-repo/semantics/openAccessSOARES,Priscilla Barbosa FerreiraROCHA,Aletheia MoraesREIS,Manuella Verdinelli de PaulaMOURA,Camilla Christian GomesSOARES,Carlos Joséeng2016-09-09T00:00:00Zoai:scielo:S1807-25772016000400234Revistahttps://www.revodontolunesp.com.br/PUBhttps://old.scielo.br/oai/scielo-oai.php||adriana@foar.unesp.br1807-25770101-1774opendoar:2016-09-09T00:00Revista de Odontologia da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study
title Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study
spellingShingle Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study
SOARES,Priscilla Barbosa Ferreira
Tooth avulsion
coconut water
cell viability
title_short Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study
title_full Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study
title_fullStr Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study
title_full_unstemmed Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study
title_sort Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study
author SOARES,Priscilla Barbosa Ferreira
author_facet SOARES,Priscilla Barbosa Ferreira
ROCHA,Aletheia Moraes
REIS,Manuella Verdinelli de Paula
MOURA,Camilla Christian Gomes
SOARES,Carlos José
author_role author
author2 ROCHA,Aletheia Moraes
REIS,Manuella Verdinelli de Paula
MOURA,Camilla Christian Gomes
SOARES,Carlos José
author2_role author
author
author
author
dc.contributor.author.fl_str_mv SOARES,Priscilla Barbosa Ferreira
ROCHA,Aletheia Moraes
REIS,Manuella Verdinelli de Paula
MOURA,Camilla Christian Gomes
SOARES,Carlos José
dc.subject.por.fl_str_mv Tooth avulsion
coconut water
cell viability
topic Tooth avulsion
coconut water
cell viability
description Abstract Objective This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO3), (3) Triethanolamine (C6H15NO3), (4) 2-Amino-2-Methil-1-Propanol (C4H11NO). Fibroblasts were plated at 2×104/ well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT formazan method. Data were analyzed by 3-way ANOVA followed by Tukey’s and Dunnet’s test. Result There are no significant effect on the cell viability regarding type of CW, period of evaluation, and the interactions between CW and period of evaluation, CW and pH adjustment method, pH adjustment method and period of evaluation (p>0.05). Conclusion The product used for CW pH adjustment did not influenced HFF viability, thought there are a tendency of better performance in natural CW.
publishDate 2016
dc.date.none.fl_str_mv 2016-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-25772016000400234
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-25772016000400234
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1807-2577.09216
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Estadual Paulista Júlio de Mesquita Filho
publisher.none.fl_str_mv Universidade Estadual Paulista Júlio de Mesquita Filho
dc.source.none.fl_str_mv Revista de Odontologia da UNESP v.45 n.4 2016
reponame:Revista de Odontologia da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Revista de Odontologia da UNESP
collection Revista de Odontologia da UNESP
repository.name.fl_str_mv Revista de Odontologia da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv ||adriana@foar.unesp.br
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