PHOTOBIOMODULATORY EFFECT OF LOW-INTENSITY LASER RADIATION ON MULTICELLULAR SPHEROIDS
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Brasileira Multidisciplinar |
Texto Completo: | http://revistarebram.com/index.php/revistauniara/article/view/1136 |
Resumo: | Photobiomodulatory effects of low-intensity laser radiation (LILR) in cells cultured in standard, two-dimensional conditions are well established. Conversely, the characteristics of this effect in three-dimensional (3D) cultures, which are currently recommended due to the greater similarity with cellular behavior in vivo, have not yet been widely investigated. The objective of this work was to analyze the biomodulator effect of LILR, on the wavelength (λ) of 685 nm, on the constitution process and on the viability of cells cultured as multicellular spheroid (MSs). For this, agarose molds containing microwells were seeded (2x105 cell/ml) with osteogenic precursor cells (OPCs - MC3T3-E1) and kept under ideal culture conditions. The molds were irradiated for five consecutive days with doses of 0.5, 1.0 and 1.5 J/cm², the first irradiation being performed immediately after sowing. The process of constitution of MSs was analyzed and the cultures were submitted to the cell viability test. The results demonstrated that the LILR at λ 685 nm exerted a dose-dependent biomodulatory effect on cell metabolism and on the process of constituting the MSs of OPCs. These results demonstrate the potential of photobiomodulation to contribute to the process of constituting MSs, which can be explored in the strategies of multicellular spheroids therapy used in regenerative medicine and bioprinting. |
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PHOTOBIOMODULATORY EFFECT OF LOW-INTENSITY LASER RADIATION ON MULTICELLULAR SPHEROIDSPhotobiomodulatory effect of low-intensity laser radiation on multicellular spheroidsthree-dimensional cell culturemulticellular spheroidslow intensity laser therapylaser radiationbiomodulatory effectcultura celular tridimensionalesferoides multicelularesterapia laser de baixa intensidaderadiação laserefeito biomodulatórioPhotobiomodulatory effects of low-intensity laser radiation (LILR) in cells cultured in standard, two-dimensional conditions are well established. Conversely, the characteristics of this effect in three-dimensional (3D) cultures, which are currently recommended due to the greater similarity with cellular behavior in vivo, have not yet been widely investigated. The objective of this work was to analyze the biomodulator effect of LILR, on the wavelength (λ) of 685 nm, on the constitution process and on the viability of cells cultured as multicellular spheroid (MSs). For this, agarose molds containing microwells were seeded (2x105 cell/ml) with osteogenic precursor cells (OPCs - MC3T3-E1) and kept under ideal culture conditions. The molds were irradiated for five consecutive days with doses of 0.5, 1.0 and 1.5 J/cm², the first irradiation being performed immediately after sowing. The process of constitution of MSs was analyzed and the cultures were submitted to the cell viability test. The results demonstrated that the LILR at λ 685 nm exerted a dose-dependent biomodulatory effect on cell metabolism and on the process of constituting the MSs of OPCs. These results demonstrate the potential of photobiomodulation to contribute to the process of constituting MSs, which can be explored in the strategies of multicellular spheroids therapy used in regenerative medicine and bioprinting.Os efeitos biomoduladores da radiação laser de baixa intensidade (RLBI) em células cultivadas em condição bidimensional padrão já estão bem estabelecidos. Em contrapartida, as características desse efeito em cultivos tridimensionais (3D), atualmente recomendados devido à maior similaridade com o comportamento celular in vivo, ainda não foram amplamente investigados. O objetivo deste trabalho foi analisar o efeito biomodulador da RLBI, no comprimento de onda (λ) de 685 nm, no processo de constituição e na viabilidade de células cultivadas como esferoides multicelulares (EMs). Para tal, moldes de agarose contendo micropoços foram semeados (2x105 células/ml) com células precursoras osteogênicas (CPOs - MC3T3-E1) e mantidas sob condições ideais de cultivo. Os moldes foram irradiados por cinco dias consecutivos com doses de 0.5, 1.0 e 1.5 J/cm², sendo a primeira irradiação realizada imediatamente após a semeadura. Foram analisadas o processo de constituição dos EMs e os cultivos foram submetidas ao ensaio de viabilidade celular. Os resultados demonstraram que a RLBI no λ 685 nm exerceu um efeito biomodulador dose-dependente sobre o metabolismo celular e sobre o processo de constituição dos EMs de CPOs. Estes resultados evidenciam a potencialidade da fotobiomodulação em contribuir com o processo de constituição dos EMs, podendo ser explorado nas estratégias de terapia celular utilizadas em medicina regenerativa e bioimpressão. Revista Brasileira Multidisciplinar - ReBraM2021-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://revistarebram.com/index.php/revistauniara/article/view/113610.25061/2527-2675/ReBraM/2021.v24i2.1136Revista Brasileira Multidisciplinar - ReBraM; v. 24 n. 2 (2021): Maio-Agosto; 156-1672527-26751415-3580reponame:Revista Brasileira Multidisciplinarinstname:Universidade de Araraquara (UNIARA)instacron:UNIARAporhttp://revistarebram.com/index.php/revistauniara/article/view/1136/703Copyright (c) 2021 Revista Brasileira Multidisciplinarhttps://creativecommons.org/licenses/by-nd/4.0info:eu-repo/semantics/openAccessGomes Cardoso Gastaldi, GabrielaPaula de Oliveira, JulianaVicente Lopes da Silva, Jorge Alvarenga Rezende, Rodrigo Capaldo Amaral, André2021-09-13T15:14:21Zoai:ojs.revistarebram.com:article/1136Revistahttp://revistarebram.com/index.php/revistauniaraPRIhttps://revistarebram.com/index.php/revistauniara/oairevistauniara@uniara.com||2527-26752527-2675opendoar:2021-09-13T15:14:21Revista Brasileira Multidisciplinar - Universidade de Araraquara (UNIARA)false |
dc.title.none.fl_str_mv |
PHOTOBIOMODULATORY EFFECT OF LOW-INTENSITY LASER RADIATION ON MULTICELLULAR SPHEROIDS Photobiomodulatory effect of low-intensity laser radiation on multicellular spheroids |
title |
PHOTOBIOMODULATORY EFFECT OF LOW-INTENSITY LASER RADIATION ON MULTICELLULAR SPHEROIDS |
spellingShingle |
PHOTOBIOMODULATORY EFFECT OF LOW-INTENSITY LASER RADIATION ON MULTICELLULAR SPHEROIDS Gomes Cardoso Gastaldi, Gabriela three-dimensional cell culture multicellular spheroids low intensity laser therapy laser radiation biomodulatory effect cultura celular tridimensional esferoides multicelulares terapia laser de baixa intensidade radiação laser efeito biomodulatório |
title_short |
PHOTOBIOMODULATORY EFFECT OF LOW-INTENSITY LASER RADIATION ON MULTICELLULAR SPHEROIDS |
title_full |
PHOTOBIOMODULATORY EFFECT OF LOW-INTENSITY LASER RADIATION ON MULTICELLULAR SPHEROIDS |
title_fullStr |
PHOTOBIOMODULATORY EFFECT OF LOW-INTENSITY LASER RADIATION ON MULTICELLULAR SPHEROIDS |
title_full_unstemmed |
PHOTOBIOMODULATORY EFFECT OF LOW-INTENSITY LASER RADIATION ON MULTICELLULAR SPHEROIDS |
title_sort |
PHOTOBIOMODULATORY EFFECT OF LOW-INTENSITY LASER RADIATION ON MULTICELLULAR SPHEROIDS |
author |
Gomes Cardoso Gastaldi, Gabriela |
author_facet |
Gomes Cardoso Gastaldi, Gabriela Paula de Oliveira, Juliana Vicente Lopes da Silva, Jorge Alvarenga Rezende, Rodrigo Capaldo Amaral, André |
author_role |
author |
author2 |
Paula de Oliveira, Juliana Vicente Lopes da Silva, Jorge Alvarenga Rezende, Rodrigo Capaldo Amaral, André |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Gomes Cardoso Gastaldi, Gabriela Paula de Oliveira, Juliana Vicente Lopes da Silva, Jorge Alvarenga Rezende, Rodrigo Capaldo Amaral, André |
dc.subject.por.fl_str_mv |
three-dimensional cell culture multicellular spheroids low intensity laser therapy laser radiation biomodulatory effect cultura celular tridimensional esferoides multicelulares terapia laser de baixa intensidade radiação laser efeito biomodulatório |
topic |
three-dimensional cell culture multicellular spheroids low intensity laser therapy laser radiation biomodulatory effect cultura celular tridimensional esferoides multicelulares terapia laser de baixa intensidade radiação laser efeito biomodulatório |
description |
Photobiomodulatory effects of low-intensity laser radiation (LILR) in cells cultured in standard, two-dimensional conditions are well established. Conversely, the characteristics of this effect in three-dimensional (3D) cultures, which are currently recommended due to the greater similarity with cellular behavior in vivo, have not yet been widely investigated. The objective of this work was to analyze the biomodulator effect of LILR, on the wavelength (λ) of 685 nm, on the constitution process and on the viability of cells cultured as multicellular spheroid (MSs). For this, agarose molds containing microwells were seeded (2x105 cell/ml) with osteogenic precursor cells (OPCs - MC3T3-E1) and kept under ideal culture conditions. The molds were irradiated for five consecutive days with doses of 0.5, 1.0 and 1.5 J/cm², the first irradiation being performed immediately after sowing. The process of constitution of MSs was analyzed and the cultures were submitted to the cell viability test. The results demonstrated that the LILR at λ 685 nm exerted a dose-dependent biomodulatory effect on cell metabolism and on the process of constituting the MSs of OPCs. These results demonstrate the potential of photobiomodulation to contribute to the process of constituting MSs, which can be explored in the strategies of multicellular spheroids therapy used in regenerative medicine and bioprinting. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://revistarebram.com/index.php/revistauniara/article/view/1136 10.25061/2527-2675/ReBraM/2021.v24i2.1136 |
url |
http://revistarebram.com/index.php/revistauniara/article/view/1136 |
identifier_str_mv |
10.25061/2527-2675/ReBraM/2021.v24i2.1136 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://revistarebram.com/index.php/revistauniara/article/view/1136/703 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Revista Brasileira Multidisciplinar https://creativecommons.org/licenses/by-nd/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Revista Brasileira Multidisciplinar https://creativecommons.org/licenses/by-nd/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Revista Brasileira Multidisciplinar - ReBraM |
publisher.none.fl_str_mv |
Revista Brasileira Multidisciplinar - ReBraM |
dc.source.none.fl_str_mv |
Revista Brasileira Multidisciplinar - ReBraM; v. 24 n. 2 (2021): Maio-Agosto; 156-167 2527-2675 1415-3580 reponame:Revista Brasileira Multidisciplinar instname:Universidade de Araraquara (UNIARA) instacron:UNIARA |
instname_str |
Universidade de Araraquara (UNIARA) |
instacron_str |
UNIARA |
institution |
UNIARA |
reponame_str |
Revista Brasileira Multidisciplinar |
collection |
Revista Brasileira Multidisciplinar |
repository.name.fl_str_mv |
Revista Brasileira Multidisciplinar - Universidade de Araraquara (UNIARA) |
repository.mail.fl_str_mv |
revistauniara@uniara.com|| |
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1797174607141142528 |