Atividades antiulcerogênica gástrica e antiinflamatória intestinal de Albarema cochliacarpos

Detalhes bibliográficos
Autor(a) principal: Silva, Maria Silene da
Data de Publicação: 2010
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da Universidade Estadual de Campinas (UNICAMP)
Texto Completo: https://hdl.handle.net/20.500.12733/1611471
Resumo: Orientador: Alba Regina Monteiro Souza Brito
id UNICAMP-30_1a628ea9663037a20dc48453ddbbef4e
oai_identifier_str oai::477967
network_acronym_str UNICAMP-30
network_name_str Biblioteca Digital de Teses e Dissertações da Universidade Estadual de Campinas (UNICAMP)
repository_id_str
spelling Atividades antiulcerogênica gástrica e antiinflamatória intestinal de Albarema cochliacarposAntiiulcerogenic gastric and anti-inflammatory intestinal activities of Albarema cochliacarposAbarema cochliacarposÚlcera gástricaColitePolifenóisAbarema cochliacarposGastric ulcerColitisPolyphenolsOrientador: Alba Regina Monteiro Souza BritoTese (doutorado) - Universidade Estadual de Campinas, Instituto de BiologiaAbstract: Abarema cochliacarpos (Gomes) Barneby & Grimes, which belongs the Mimosaceae family, is popularly known as "barbatimao" by the tradicional communities in Northeastern Brazil that use its bark in the treatment of gastric ulcer and inflammation, among other uses. In this work, the antiulcerogenic activity of its extracts and fractions was screened, using the gastric ulcer model induced by absolute ethanol in rats and selecting of the butanolic fraction of the methanolic extracts at dosis of 150 mg/kg as being in the most effective one. The action mechanisms of this fraction were studied based on the mucus gastric content and gastric secretion models. We have also decided to test it in anti-inflammatory intestinal activity model. Acute toxicity of AC150 was evaluated in Swiss mice, and no symptoms or visible toxicity signs were observed. Wistar rats were used in the others experiments. The intraduodenal pathway was utilized only in the pylorus ligature model; in the other models the oral pathway was employed. The AC 150 was able to significantly increased the mucus production, as well as to change the biochemical parameters of the acid gastric secretion, reducing the gastric volume and increasing the pH, suggesting a cytoprotection and anti-secretory activity. AC150 showed healing activity in the chronic model of gastric ulcer induced by 30 % acetic acid. This was demonstrated by the reduction of lesion area, in agreement with HE histological analysis. The induction of cellular proliferation was indicated by the PCNA results. The immunolocation of HSP-70, COX-2 and VEGF in the gastric tissue suggested that the ulcer cicatrization activity was induced by the increased of the expression of these factors, as well as the increase of the mucus production highlightened in the PAS results. In the acute ulcerative colitis model induced by intra-colonic administration of trinitrobenzensulfonic acid (TNBS) (10 mg/animal) AC 100 and 150 mg/kg were able to significantly decrease the lesion and the mieloperoxidase (MPO) levels in the colonic mucosa. However, only AC150 decreased the levels of the tumour necrosis factor-alpha (TNF-a). The western blot analysis showed that AC 150 mg/kg induced the down-regulation of both cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) expression, and the involvement of signaling also demonstrated a reduction in the JNK activation in this dosis. In the chronic colitis model (TNBS, 30 mg/animal) AC 150 showed the same inhibition profile of the inflammatory parameters; nevertheless, the increase of the antiinflammatory cytokine production IL-10 was highlightened only in this model. These data suggested a significant protective effect of the chronic administration of AC during the chronic phase of the inflammatory response associated with TNBS induced colitis. Phytochemical studies by mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR) revealed that catechins were a major component into condensate class of tannins. Taken as a whole, these data allow us to conclude that the AC150 of Abarema cochliacarpos has chemical compounds that present antiulcerogenic gastric and anti-inflammatory activities, which may be attributed to the tannins and (+)-catechins of this fraction.Abstract: Abarema cochliacarpos (Gomes) Barneby & Grimes, which belongs the Mimosaceae family, is popularly known as "barbatimao" by the tradicional communities in Northeastern Brazil that use its bark in the treatment of gastric ulcer and inflammation, among other uses. In this work, the antiulcerogenic activity of its extracts and fractions was screened, using the gastric ulcer model induced by absolute ethanol in rats and selecting of the butanolic fraction of the methanolic extracts at dosis of 150 mg/kg as being in the most effective one. The action mechanisms of this fraction were studied based on the mucus gastric content and gastric secretion models. We have also decided to test it in anti-inflammatory intestinal activity model. Acute toxicity of AC150 was evaluated in Swiss mice, and no symptoms or visible toxicity signs were observed. Wistar rats were used in the others experiments. The intraduodenal pathway was utilized only in the pylorus ligature model; in the other models the oral pathway was employed. The AC 150 was able to significantly increased the mucus production, as well as to change the biochemical parameters of the acid gastric secretion, reducing the gastric volume and increasing the pH, suggesting a cytoprotection and anti-secretory activity. AC150 showed healing activity in the chronic model of gastric ulcer induced by 30 % acetic acid. This was demonstrated by the reduction of lesion area, in agreement with HE histological analysis. The induction of cellular proliferation was indicated by the PCNA results. The immunolocation of HSP-70, COX-2 and VEGF in the gastric tissue suggested that the ulcer cicatrization activity was induced by the increased of the expression of these factors, as well as the increase of the mucus production highlightened in the PAS results. In the acute ulcerative colitis model induced by intra-colonic administration of trinitrobenzensulfonic acid (TNBS) (10 mg/animal) AC 100 and 150 mg/kg were able to significantly decrease the lesion and the mieloperoxidase (MPO) levels in the colonic mucosa. However, only AC150 decreased the levels of the tumour necrosis factor-alpha (TNF-a). The western blot analysis showed that AC 150 mg/kg induced the down-regulation of both cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) expression, and the involvement of signaling also demonstrated a reduction in the JNK activation in this dosis. In the chronic colitis model (TNBS, 30 mg/animal) AC 150 showed the same inhibition profile of the inflammatory parameters; nevertheless, the increase of the antiinflammatory cytokine production IL-10 was highlightened only in this model. These data suggested a significant protective effect of the chronic administration of AC during the chronic phase of the inflammatory response associated with TNBS induced colitis. Phytochemical studies by mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR) revealed that catechins were a major component into condensate class of tannins. Taken as a whole, these data allow us to conclude that the AC150 of Abarema cochliacarpos has chemical compounds that present antiulcerogenic gastric and anti-inflammatory activities, which may be attributed to the tannins and (+)-catechins of this fraction.DoutoradoFisiologiaDoutor em Biologia Funcional e Molecular[s.n.]Brito, Alba Regina Monteiro Souza, 1954-Areas, Miguel ArcanjoBatista, Leonia MariaCola, MariaSalvador, Marcos JoséUniversidade Estadual de Campinas (UNICAMP). Instituto de BiologiaPrograma de Pós-Graduação em Biologia Funcional e MolecularUNIVERSIDADE ESTADUAL DE CAMPINASSilva, Maria Silene da2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdf107 p. : il.https://hdl.handle.net/20.500.12733/1611471SILVA, Maria Silene da. Atividades antiulcerogênica gástrica e antiinflamatória intestinal de Albarema cochliacarpos. 2010. 107 p. Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: https://hdl.handle.net/20.500.12733/1611471. Acesso em: 3 set. 2024.https://repositorio.unicamp.br/acervo/detalhe/477967porreponame:Biblioteca Digital de Teses e Dissertações da Universidade Estadual de Campinas (UNICAMP)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPinfo:eu-repo/semantics/openAccess2017-02-18T05:48:07Zoai::477967Biblioteca Digital de Teses e DissertaçõesPUBhttp://repositorio.unicamp.br/oai/tese/oai.aspsbubd@unicamp.bropendoar:2017-02-18T05:48:07Biblioteca Digital de Teses e Dissertações da Universidade Estadual de Campinas (UNICAMP) - Universidade Estadual de Campinas (UNICAMP)false
dc.title.none.fl_str_mv Atividades antiulcerogênica gástrica e antiinflamatória intestinal de Albarema cochliacarpos
Antiiulcerogenic gastric and anti-inflammatory intestinal activities of Albarema cochliacarpos
title Atividades antiulcerogênica gástrica e antiinflamatória intestinal de Albarema cochliacarpos
spellingShingle Atividades antiulcerogênica gástrica e antiinflamatória intestinal de Albarema cochliacarpos
Silva, Maria Silene da
Abarema cochliacarpos
Úlcera gástrica
Colite
Polifenóis
Abarema cochliacarpos
Gastric ulcer
Colitis
Polyphenols
title_short Atividades antiulcerogênica gástrica e antiinflamatória intestinal de Albarema cochliacarpos
title_full Atividades antiulcerogênica gástrica e antiinflamatória intestinal de Albarema cochliacarpos
title_fullStr Atividades antiulcerogênica gástrica e antiinflamatória intestinal de Albarema cochliacarpos
title_full_unstemmed Atividades antiulcerogênica gástrica e antiinflamatória intestinal de Albarema cochliacarpos
title_sort Atividades antiulcerogênica gástrica e antiinflamatória intestinal de Albarema cochliacarpos
author Silva, Maria Silene da
author_facet Silva, Maria Silene da
author_role author
dc.contributor.none.fl_str_mv Brito, Alba Regina Monteiro Souza, 1954-
Areas, Miguel Arcanjo
Batista, Leonia Maria
Cola, Maria
Salvador, Marcos José
Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia
Programa de Pós-Graduação em Biologia Funcional e Molecular
UNIVERSIDADE ESTADUAL DE CAMPINAS
dc.contributor.author.fl_str_mv Silva, Maria Silene da
dc.subject.por.fl_str_mv Abarema cochliacarpos
Úlcera gástrica
Colite
Polifenóis
Abarema cochliacarpos
Gastric ulcer
Colitis
Polyphenols
topic Abarema cochliacarpos
Úlcera gástrica
Colite
Polifenóis
Abarema cochliacarpos
Gastric ulcer
Colitis
Polyphenols
description Orientador: Alba Regina Monteiro Souza Brito
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/20.500.12733/1611471
SILVA, Maria Silene da. Atividades antiulcerogênica gástrica e antiinflamatória intestinal de Albarema cochliacarpos. 2010. 107 p. Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: https://hdl.handle.net/20.500.12733/1611471. Acesso em: 3 set. 2024.
url https://hdl.handle.net/20.500.12733/1611471
identifier_str_mv SILVA, Maria Silene da. Atividades antiulcerogênica gástrica e antiinflamatória intestinal de Albarema cochliacarpos. 2010. 107 p. Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia, Campinas, SP. Disponível em: https://hdl.handle.net/20.500.12733/1611471. Acesso em: 3 set. 2024.
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://repositorio.unicamp.br/acervo/detalhe/477967
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
107 p. : il.
dc.publisher.none.fl_str_mv [s.n.]
publisher.none.fl_str_mv [s.n.]
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da Universidade Estadual de Campinas (UNICAMP)
instname:Universidade Estadual de Campinas (UNICAMP)
instacron:UNICAMP
instname_str Universidade Estadual de Campinas (UNICAMP)
instacron_str UNICAMP
institution UNICAMP
reponame_str Biblioteca Digital de Teses e Dissertações da Universidade Estadual de Campinas (UNICAMP)
collection Biblioteca Digital de Teses e Dissertações da Universidade Estadual de Campinas (UNICAMP)
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da Universidade Estadual de Campinas (UNICAMP) - Universidade Estadual de Campinas (UNICAMP)
repository.mail.fl_str_mv sbubd@unicamp.br
_version_ 1809189025625407488

Registros relacionados