Chronic administration of Abarema cochliacarpos attenuates colonic inflammation in rats

Detalhes bibliográficos
Autor(a) principal: Silva,Maria Silene da
Data de Publicação: 2011
Outros Autores: Sánchez-Fidalgo,Susana, Cárdeno,Ana, Talero,Elena, Silva,Marcelo Aparecido da, Vilegas,Wagner, Brito,Alba R. M. Souza, Lastra,Catalina A. de la
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista Brasileira de Farmacognosia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000400018
Resumo: Inflammatory bowel diseases are characterized by a chronic clinical course of relapse and remission associated with self-destructive inflammation of the gastrointestinal tract. Active extracts from plants have emerged as natural potential candidates for its treatment. Abarema cochliacarpos (Gomes) Barneby & Grimes, Fabaceae (Barbatimão), is a native medicinal plant in to Brazil. Previously we have demonstrated in an acute colitis model a marked protective effect of a butanolic extract, so we decided to assess its anti-inflammatory effect in a chronic ulcerative colitis model induced by trinitrobenzensulfonic acid (TNBS). Abarema cochliacarpos (150 mg/day, v.o.) was administered for fourteen consecutive days. This treatment decreased significantly macroscopic damage as compared with TNBS. Histological analysis showed that the extract improved the microscopic structure. Myeloperoxidase activity (MPO) was significantly decreased. Study of cytokines showed that TNF-α was diminished and IL-10 level was increased after Abarema cochliacarpos treatment. In order to elucidate inflammatory mechanisms, expression of cyclooxygenase (COX)-2 and nitric oxide synthase (iNOS) were studied showing a significant downregulation. In addition, there was reduction in the JNK and p-38 activation. Finally, IκB degradation was blocked by Abarema cochliacarpos treatment being consistent with an up-regulation of the NF-kappaB-binding activity. These results reinforce the anti-inflammatory effects described previously suggesting that Abarema cochliacarpos could provide a source for the search for new anti-inflammatory compounds useful in ulcerative colitis treatment.
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spelling Chronic administration of Abarema cochliacarpos attenuates colonic inflammation in ratsAbarema cochliacarposchronic colitisinhibitor protein kappa BJNKp-38trinitrobenzensulfonic acidInflammatory bowel diseases are characterized by a chronic clinical course of relapse and remission associated with self-destructive inflammation of the gastrointestinal tract. Active extracts from plants have emerged as natural potential candidates for its treatment. Abarema cochliacarpos (Gomes) Barneby & Grimes, Fabaceae (Barbatimão), is a native medicinal plant in to Brazil. Previously we have demonstrated in an acute colitis model a marked protective effect of a butanolic extract, so we decided to assess its anti-inflammatory effect in a chronic ulcerative colitis model induced by trinitrobenzensulfonic acid (TNBS). Abarema cochliacarpos (150 mg/day, v.o.) was administered for fourteen consecutive days. This treatment decreased significantly macroscopic damage as compared with TNBS. Histological analysis showed that the extract improved the microscopic structure. Myeloperoxidase activity (MPO) was significantly decreased. Study of cytokines showed that TNF-α was diminished and IL-10 level was increased after Abarema cochliacarpos treatment. In order to elucidate inflammatory mechanisms, expression of cyclooxygenase (COX)-2 and nitric oxide synthase (iNOS) were studied showing a significant downregulation. In addition, there was reduction in the JNK and p-38 activation. Finally, IκB degradation was blocked by Abarema cochliacarpos treatment being consistent with an up-regulation of the NF-kappaB-binding activity. These results reinforce the anti-inflammatory effects described previously suggesting that Abarema cochliacarpos could provide a source for the search for new anti-inflammatory compounds useful in ulcerative colitis treatment.Sociedade Brasileira de Farmacognosia2011-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000400018Revista Brasileira de Farmacognosia v.21 n.4 2011reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1590/S0102-695X2011005000122info:eu-repo/semantics/openAccessSilva,Maria Silene daSánchez-Fidalgo,SusanaCárdeno,AnaTalero,ElenaSilva,Marcelo Aparecido daVilegas,WagnerBrito,Alba R. M. SouzaLastra,Catalina A. de lapor2011-08-15T00:00:00Zoai:scielo:S0102-695X2011000400018Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2011-08-15T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false
dc.title.none.fl_str_mv Chronic administration of Abarema cochliacarpos attenuates colonic inflammation in rats
title Chronic administration of Abarema cochliacarpos attenuates colonic inflammation in rats
spellingShingle Chronic administration of Abarema cochliacarpos attenuates colonic inflammation in rats
Silva,Maria Silene da
Abarema cochliacarpos
chronic colitis
inhibitor protein kappa B
JNK
p-38
trinitrobenzensulfonic acid
title_short Chronic administration of Abarema cochliacarpos attenuates colonic inflammation in rats
title_full Chronic administration of Abarema cochliacarpos attenuates colonic inflammation in rats
title_fullStr Chronic administration of Abarema cochliacarpos attenuates colonic inflammation in rats
title_full_unstemmed Chronic administration of Abarema cochliacarpos attenuates colonic inflammation in rats
title_sort Chronic administration of Abarema cochliacarpos attenuates colonic inflammation in rats
author Silva,Maria Silene da
author_facet Silva,Maria Silene da
Sánchez-Fidalgo,Susana
Cárdeno,Ana
Talero,Elena
Silva,Marcelo Aparecido da
Vilegas,Wagner
Brito,Alba R. M. Souza
Lastra,Catalina A. de la
author_role author
author2 Sánchez-Fidalgo,Susana
Cárdeno,Ana
Talero,Elena
Silva,Marcelo Aparecido da
Vilegas,Wagner
Brito,Alba R. M. Souza
Lastra,Catalina A. de la
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva,Maria Silene da
Sánchez-Fidalgo,Susana
Cárdeno,Ana
Talero,Elena
Silva,Marcelo Aparecido da
Vilegas,Wagner
Brito,Alba R. M. Souza
Lastra,Catalina A. de la
dc.subject.por.fl_str_mv Abarema cochliacarpos
chronic colitis
inhibitor protein kappa B
JNK
p-38
trinitrobenzensulfonic acid
topic Abarema cochliacarpos
chronic colitis
inhibitor protein kappa B
JNK
p-38
trinitrobenzensulfonic acid
description Inflammatory bowel diseases are characterized by a chronic clinical course of relapse and remission associated with self-destructive inflammation of the gastrointestinal tract. Active extracts from plants have emerged as natural potential candidates for its treatment. Abarema cochliacarpos (Gomes) Barneby & Grimes, Fabaceae (Barbatimão), is a native medicinal plant in to Brazil. Previously we have demonstrated in an acute colitis model a marked protective effect of a butanolic extract, so we decided to assess its anti-inflammatory effect in a chronic ulcerative colitis model induced by trinitrobenzensulfonic acid (TNBS). Abarema cochliacarpos (150 mg/day, v.o.) was administered for fourteen consecutive days. This treatment decreased significantly macroscopic damage as compared with TNBS. Histological analysis showed that the extract improved the microscopic structure. Myeloperoxidase activity (MPO) was significantly decreased. Study of cytokines showed that TNF-α was diminished and IL-10 level was increased after Abarema cochliacarpos treatment. In order to elucidate inflammatory mechanisms, expression of cyclooxygenase (COX)-2 and nitric oxide synthase (iNOS) were studied showing a significant downregulation. In addition, there was reduction in the JNK and p-38 activation. Finally, IκB degradation was blocked by Abarema cochliacarpos treatment being consistent with an up-regulation of the NF-kappaB-binding activity. These results reinforce the anti-inflammatory effects described previously suggesting that Abarema cochliacarpos could provide a source for the search for new anti-inflammatory compounds useful in ulcerative colitis treatment.
publishDate 2011
dc.date.none.fl_str_mv 2011-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000400018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2011000400018
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.1590/S0102-695X2011005000122
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
publisher.none.fl_str_mv Sociedade Brasileira de Farmacognosia
dc.source.none.fl_str_mv Revista Brasileira de Farmacognosia v.21 n.4 2011
reponame:Revista Brasileira de Farmacognosia (Online)
instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron:SBFGNOSIA
instname_str Sociedade Brasileira de Farmacognosia (SBFgnosia)
instacron_str SBFGNOSIA
institution SBFGNOSIA
reponame_str Revista Brasileira de Farmacognosia (Online)
collection Revista Brasileira de Farmacognosia (Online)
repository.name.fl_str_mv Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)
repository.mail.fl_str_mv rbgnosia@ltf.ufpb.br
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