MICROPROPAGATION OF Aechmea setigera MART. EX SCHULT. & SCHULT. F.: A ENDEMIC BROMELIAD FROM WESTERN AMAZON

Detalhes bibliográficos
Autor(a) principal: Leão, João Ricardo Avelino
Data de Publicação: 2014
Outros Autores: Vasconcelos, Janaína Medeiros, Beltrão, Renata Teixeira, Raposo, Andrea, Fermino Junior, Paulo Cesar Poeta
Tipo de documento: Artigo
Idioma: por
Título da fonte: Biota Amazônia
Texto Completo: https://periodicos.unifap.br/index.php/biota/article/view/1030
Resumo: Bromeliads in the Amazon are generally unfamiliar. Aechmea setigera is endemic bromeliad from Amazon with ornamental potential. The objective of this work was to evaluate the physiological responses under the effect of growth regulators on the steps of micropropagation as well as establish a protocol as support for conservation. Seedlings germinated and grown in vitro were inoculated in MS liquid stationary culture, plus 6-benzylaminopurine (BAP) in different concentrations (0, 2.2, 4.4, 8.8 and 17.6 μM). For rooting, microshoots were transferred to MS medium with 0, 2.4, 4.9, 9.8 μM indole acetic acid (IAA) and indole butyric acid (IBA). After five subcultures, the use of 17.6 μM BAP showed the highest number and shorter length of adventitious shoots. The starch content was lower in shoots induced with lower concentrations of BAP. The use of indole butyric acid at concentrations 4.9 and 9.8 μM promotes the highest number of regenerated adventitious roots. Regenerated plantlets were acclimatized (99 %) using commercial substrate in environment with 50% shading. A. setigera micropropagation is a viable strategy for plantlets production and species conservation.Keywords: Bromeliaceae; in vitro propagation; starch contents; Western Amazon.
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spelling MICROPROPAGATION OF Aechmea setigera MART. EX SCHULT. & SCHULT. F.: A ENDEMIC BROMELIAD FROM WESTERN AMAZONMICROPROPAGAÇÃO DE Aechmea setigera Mart. ex Schult. & Schult. f.: UMA BROMÉLIA ENDÊMICA DA AMAZÔNIA OCIDENTALBromeliads in the Amazon are generally unfamiliar. Aechmea setigera is endemic bromeliad from Amazon with ornamental potential. The objective of this work was to evaluate the physiological responses under the effect of growth regulators on the steps of micropropagation as well as establish a protocol as support for conservation. Seedlings germinated and grown in vitro were inoculated in MS liquid stationary culture, plus 6-benzylaminopurine (BAP) in different concentrations (0, 2.2, 4.4, 8.8 and 17.6 μM). For rooting, microshoots were transferred to MS medium with 0, 2.4, 4.9, 9.8 μM indole acetic acid (IAA) and indole butyric acid (IBA). After five subcultures, the use of 17.6 μM BAP showed the highest number and shorter length of adventitious shoots. The starch content was lower in shoots induced with lower concentrations of BAP. The use of indole butyric acid at concentrations 4.9 and 9.8 μM promotes the highest number of regenerated adventitious roots. Regenerated plantlets were acclimatized (99 %) using commercial substrate in environment with 50% shading. A. setigera micropropagation is a viable strategy for plantlets production and species conservation.Keywords: Bromeliaceae; in vitro propagation; starch contents; Western Amazon.As bromélias da Amazônia são em geral pouco conhecidas. Aechmea setigera é uma bromélia endêmica da Amazônia com potencial ornamental. O objetivo do trabalho foi avaliar as respostas fisiológicas sob efeito de reguladores de crescimento nas etapas da micropropagação, bem como, estabelecer um protocolo como subsídio para a conservação. Plântulas germinadas e desenvolvidas in vitro foram inoculadas em meio de cultura MS líquidas estacionário acrescidas de 6-benzilaminopurina (BAP) em diferentes concentrações (0; 2,2; 4,4; 8,8 e 17,6 μM). Para o enraizamento, microbrotos foram transferidos para meio de cultura MS com 0; 2,4; 4,9; 9,8µM de ácido indolacético (AIA) e ácido indolbutírico (AIB). Após cinco subcultivos, o uso de 17,6 μM de BAP apresentou o maior número e o menor comprimento de brotações adventícias. Os teores de amido foram menores nos brotos induzidos com as menores concentrações de BAP. O uso de ácido indolbutírico nas concentrações de 4,9 e 9,8 μM promoveram o maior número de raízes adventícias regeneradas. Plantas jovens regeneradas foram aclimatizadas (99 %) utilizando substrato comercial em ambiente com 50 % de sombreamento. A micropropagação de A. setigera é uma estratégia viável para a produção de mudas e a conservação da espécie.Palavras-chave: Bromeliaceae, propagação in vitro, teores de amido, Amazônia Ocidental.Universidade Federal do AmapáCAPES e CNPqLeão, João Ricardo AvelinoVasconcelos, Janaína MedeirosBeltrão, Renata TeixeiraRaposo, AndreaFermino Junior, Paulo Cesar Poeta2014-08-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtigo Avaliado pelos Paresapplication/pdfhttps://periodicos.unifap.br/index.php/biota/article/view/103010.18561/2179-5746/biotaamazonia.v4n2p117-123Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota); v. 4, n. 2 (2014); 117-1232179-5746reponame:Biota Amazôniainstname:Universidade Federal do Amapá (UNIFAP)instacron:UNIFAPporhttps://periodicos.unifap.br/index.php/biota/article/view/1030/v4n2p117-123.pdfDireitos autorais 2014 Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota)http://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess2016-04-13T17:58:54Zoai:ojs.pkp.sfu.ca:article/1030Revistahttp://periodicos.unifap.br/index.php/biotaONGhttps://periodicos.unifap.br/index.php/biota/oai||juliosa@unifap.br2179-57462179-5746opendoar:2016-04-13T17:58:54Biota Amazônia - Universidade Federal do Amapá (UNIFAP)false
dc.title.none.fl_str_mv MICROPROPAGATION OF Aechmea setigera MART. EX SCHULT. & SCHULT. F.: A ENDEMIC BROMELIAD FROM WESTERN AMAZON
MICROPROPAGAÇÃO DE Aechmea setigera Mart. ex Schult. & Schult. f.: UMA BROMÉLIA ENDÊMICA DA AMAZÔNIA OCIDENTAL
title MICROPROPAGATION OF Aechmea setigera MART. EX SCHULT. & SCHULT. F.: A ENDEMIC BROMELIAD FROM WESTERN AMAZON
spellingShingle MICROPROPAGATION OF Aechmea setigera MART. EX SCHULT. & SCHULT. F.: A ENDEMIC BROMELIAD FROM WESTERN AMAZON
Leão, João Ricardo Avelino
title_short MICROPROPAGATION OF Aechmea setigera MART. EX SCHULT. & SCHULT. F.: A ENDEMIC BROMELIAD FROM WESTERN AMAZON
title_full MICROPROPAGATION OF Aechmea setigera MART. EX SCHULT. & SCHULT. F.: A ENDEMIC BROMELIAD FROM WESTERN AMAZON
title_fullStr MICROPROPAGATION OF Aechmea setigera MART. EX SCHULT. & SCHULT. F.: A ENDEMIC BROMELIAD FROM WESTERN AMAZON
title_full_unstemmed MICROPROPAGATION OF Aechmea setigera MART. EX SCHULT. & SCHULT. F.: A ENDEMIC BROMELIAD FROM WESTERN AMAZON
title_sort MICROPROPAGATION OF Aechmea setigera MART. EX SCHULT. & SCHULT. F.: A ENDEMIC BROMELIAD FROM WESTERN AMAZON
author Leão, João Ricardo Avelino
author_facet Leão, João Ricardo Avelino
Vasconcelos, Janaína Medeiros
Beltrão, Renata Teixeira
Raposo, Andrea
Fermino Junior, Paulo Cesar Poeta
author_role author
author2 Vasconcelos, Janaína Medeiros
Beltrão, Renata Teixeira
Raposo, Andrea
Fermino Junior, Paulo Cesar Poeta
author2_role author
author
author
author
dc.contributor.none.fl_str_mv
CAPES e CNPq
dc.contributor.author.fl_str_mv Leão, João Ricardo Avelino
Vasconcelos, Janaína Medeiros
Beltrão, Renata Teixeira
Raposo, Andrea
Fermino Junior, Paulo Cesar Poeta
description Bromeliads in the Amazon are generally unfamiliar. Aechmea setigera is endemic bromeliad from Amazon with ornamental potential. The objective of this work was to evaluate the physiological responses under the effect of growth regulators on the steps of micropropagation as well as establish a protocol as support for conservation. Seedlings germinated and grown in vitro were inoculated in MS liquid stationary culture, plus 6-benzylaminopurine (BAP) in different concentrations (0, 2.2, 4.4, 8.8 and 17.6 μM). For rooting, microshoots were transferred to MS medium with 0, 2.4, 4.9, 9.8 μM indole acetic acid (IAA) and indole butyric acid (IBA). After five subcultures, the use of 17.6 μM BAP showed the highest number and shorter length of adventitious shoots. The starch content was lower in shoots induced with lower concentrations of BAP. The use of indole butyric acid at concentrations 4.9 and 9.8 μM promotes the highest number of regenerated adventitious roots. Regenerated plantlets were acclimatized (99 %) using commercial substrate in environment with 50% shading. A. setigera micropropagation is a viable strategy for plantlets production and species conservation.Keywords: Bromeliaceae; in vitro propagation; starch contents; Western Amazon.
publishDate 2014
dc.date.none.fl_str_mv 2014-08-02
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Artigo Avaliado pelos Pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.unifap.br/index.php/biota/article/view/1030
10.18561/2179-5746/biotaamazonia.v4n2p117-123
url https://periodicos.unifap.br/index.php/biota/article/view/1030
identifier_str_mv 10.18561/2179-5746/biotaamazonia.v4n2p117-123
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.unifap.br/index.php/biota/article/view/1030/v4n2p117-123.pdf
dc.rights.driver.fl_str_mv Direitos autorais 2014 Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota)
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos autorais 2014 Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota)
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv





dc.publisher.none.fl_str_mv Universidade Federal do Amapá
publisher.none.fl_str_mv Universidade Federal do Amapá
dc.source.none.fl_str_mv Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota); v. 4, n. 2 (2014); 117-123
2179-5746
reponame:Biota Amazônia
instname:Universidade Federal do Amapá (UNIFAP)
instacron:UNIFAP
instname_str Universidade Federal do Amapá (UNIFAP)
instacron_str UNIFAP
institution UNIFAP
reponame_str Biota Amazônia
collection Biota Amazônia
repository.name.fl_str_mv Biota Amazônia - Universidade Federal do Amapá (UNIFAP)
repository.mail.fl_str_mv ||juliosa@unifap.br
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