Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed

Detalhes bibliográficos
Autor(a) principal: Costa, Diego Figueiredo da
Data de Publicação: 2021
Outros Autores: Morais, Davidianne de Andrade, Silva, Aline Ferreira da, Borges, Ismael Lira, Ferreira, Jefferson da Silva, Bezerra, Camila de Sousa, Pimenta, Carla Lauise Rodrigues Menezes, Silva, Maria Luana Cristiny Rodrigues, Azevedo, Sergio Santos de, Alves, Clebert José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/12373
Resumo: Most of the information about leptospirosis was obtained from experimental infections with rodents, which in spite of being relevant, do not provide all the answers about the disease in animals and human beings, given the variability of interactions which exist between the agent and the different hosts. Therefore, the objective of the present study was to verify the pathogenesis of the infection in native breeds of sheep of Brazil challenged with a strain of the serogroup Pomona.  A total of 10 sheep were divided into two groups with five animals according to the breed. In each group four sheep were challenged by intraperitoneal route with a strain of the serogroup Pomona and one was used as control. Sheep were monitored for 60 days, with collection of blood for serologic diagnosis, as well as vaginal fluid and urine for microbiological and molecular analyses. After this period the animals were submitted to euthanasia and necropsy, with collection of tissues for microbiological, molecular and histopathologic diagnosis. All the challenged sheep presented anti-Leptospira antibodies. Crossbred sheep presented a lower concentration of titers and the antibodies were detected for a shorter period of time when compared to the Santa Inês sheep, with statistical difference in the concentration of the titers on the days 15 (p<0.05), 45 (p<0.05) and 60 (p<0.05) post-infection. There was no significant difference between the groups when comparing the positivity rates of the microscopic agglutination test (MAT) (p>0.05). Eight positive reactions in the urine and vaginal fluid PCR were detected in both groups, being four (50%) in urine and four (50%) in the vaginal fluid, however without statistical difference (p>0.05). In both groups there was a greater proportion of PCR positive samples in kidneys (71.4%) in relation to uterus (28.6%), however without significant difference (p>0.05). There was no significant difference between the groups when comparing the positivity rates of the PCR (p>0.05). The isolation of leptospires from the urine and kidney of a crossbred sheep was possible. Therefore, it is possible that the native sheep, especially the crossbred, may have a relation of resistance with strains of the serogroup Pomona. However the intensity and duration of this relation need to be elucidated and longer-lasting investigations of natural and experimental infections are necessary in order to determine the epidemiological nature of this relation.
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spelling Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breedDinámica de la infección experimental con Leptospira serogrupo Pomona en diferentes razas de ovinos Dinâmica da infecção experimental com Leptospira sorogrupo Pomona em diferentes raças de ovinosResistanceLeptospirosisSanta InêsCrossbred sheepPomona. ResistenciaLeptospirosisSanta InêsOveja mestizaPomona.ResistênciaLeptospiroseSanta InêsOvelha cruzadaPomona.Most of the information about leptospirosis was obtained from experimental infections with rodents, which in spite of being relevant, do not provide all the answers about the disease in animals and human beings, given the variability of interactions which exist between the agent and the different hosts. Therefore, the objective of the present study was to verify the pathogenesis of the infection in native breeds of sheep of Brazil challenged with a strain of the serogroup Pomona.  A total of 10 sheep were divided into two groups with five animals according to the breed. In each group four sheep were challenged by intraperitoneal route with a strain of the serogroup Pomona and one was used as control. Sheep were monitored for 60 days, with collection of blood for serologic diagnosis, as well as vaginal fluid and urine for microbiological and molecular analyses. After this period the animals were submitted to euthanasia and necropsy, with collection of tissues for microbiological, molecular and histopathologic diagnosis. All the challenged sheep presented anti-Leptospira antibodies. Crossbred sheep presented a lower concentration of titers and the antibodies were detected for a shorter period of time when compared to the Santa Inês sheep, with statistical difference in the concentration of the titers on the days 15 (p<0.05), 45 (p<0.05) and 60 (p<0.05) post-infection. There was no significant difference between the groups when comparing the positivity rates of the microscopic agglutination test (MAT) (p>0.05). Eight positive reactions in the urine and vaginal fluid PCR were detected in both groups, being four (50%) in urine and four (50%) in the vaginal fluid, however without statistical difference (p>0.05). In both groups there was a greater proportion of PCR positive samples in kidneys (71.4%) in relation to uterus (28.6%), however without significant difference (p>0.05). There was no significant difference between the groups when comparing the positivity rates of the PCR (p>0.05). The isolation of leptospires from the urine and kidney of a crossbred sheep was possible. Therefore, it is possible that the native sheep, especially the crossbred, may have a relation of resistance with strains of the serogroup Pomona. However the intensity and duration of this relation need to be elucidated and longer-lasting investigations of natural and experimental infections are necessary in order to determine the epidemiological nature of this relation.La mayor parte de la información sobre la leptospirosis se obtuvo de infecciones experimentales con roedores, que a pesar de ser relevantes, no brindan todas las respuestas sobre la enfermedad en animales y seres humanos, dada la variabilidad de interacciones que existen entre el agente y los diferentes hospedadores. Por lo tanto, el objetivo del presente estudio fue verificar la patogénesis de la infección en razas nativas de ovejas de Brasil desafiadas con una cepa del serogrupo Pomona. Un total de 10 ovejas se dividieron en dos grupos con cinco animales según la raza. En cada grupo, cuatro ovejas fueron desafiadas por vía intraperitoneal con una cepa del serogrupo Pomona y una se usó como control. Las ovejas fueron monitoreadas durante 60 días, con recolección de sangre para diagnóstico serológico, así como fluidos vaginales y orina para análisis microbiológicos y moleculares. Luego de este período los animales fueron sometidos a eutanasia y necropsia, con recolección de tejidos para diagnóstico microbiológico, molecular e histopatológico. Todas las ovejas desafiadas presentaron anticuerpos anti-Leptospira. Los ovinos mestizos presentaron menor concentración de títulos y los anticuerpos se detectaron por un período de tiempo más corto en comparación con el ovino Santa Inês, con diferencia estadística en la concentración de los títulos en los días 15 (p <0.05), 45 (p < 0,05) y 60 (p <0,05) post-infección. No hubo diferencia significativa entre los grupos al comparar las tasas de positividad de la prueba de aglutinación microscópica (MAT) (p> 0.05). Se detectaron ocho reacciones positivas en la PCR de orina y fluido vaginal en ambos grupos, siendo cuatro (50%) en orina y cuatro (50%) en fluido vaginal, sin embargo sin diferencia estadística (p> 0.05). En ambos grupos hubo una mayor proporción de muestras positivas a PCR en riñones (71,4%) en relación al útero (28,6%), sin embargo sin diferencia significativa (p> 0,05). No hubo diferencia significativa entre los grupos al comparar las tasas de positividad de la PCR (p> 0.05). Fue posible el aislamiento de leptospiras de la orina y el riñón de una oveja mestiza. Por tanto, es posible que la oveja autóctona brasileña, especialmente la mestiza, pueda tener una relación de resistencia con cepas del serogrupo Pomona. Sin embargo, es necesario dilucidar la intensidad y duración de esta relación y se necesitan investigaciones más duraderas de las infecciones naturales y experimentales para determinar la naturaleza epidemiológica de esta relación.A maioria das informações sobre a leptospirose foi obtida a partir de infecções experimentais com roedores, que apesar de relevantes, não fornecem todas as respostas sobre a doença em animais e seres humanos, dada a variabilidade de interações existentes entre o agente e os diferentes hospedeiros. Portanto, o objetivo do presente estudo foi verificar a patogênese da infecção em raças nativas de ovinos do Brasil desafiados com uma cepa do sorogrupo Pomona. Um total de 10 ovelhas foram divididas em dois grupos com cinco animais de acordo com a raça. Em cada grupo quatro ovelhas foram desafiadas por via intraperitoneal com uma cepa do sorogrupo Pomona e uma foi utilizada como controle. As ovelhas foram acompanhadas por 60 dias, com coleta de sangue para diagnóstico sorológico, bem como de secreção vaginal e urina para análises microbiológicas e moleculares. Após esse período os animais foram submetidos à eutanásia e necropsia, com coleta de tecidos para diagnóstico microbiológico, molecular e histopatológico. Todas as ovelhas desafiadas apresentaram anticorpos anti-Leptospira. Ovinos mestiços apresentaram menor concentração de títulos e os anticorpos foram detectados por menor período de tempo quando comparados aos ovinos Santa Inês, com diferença estatística na concentração dos títulos nos dias 15 (p <0,05), 45 (p < 0,05) e 60 (p <0,05) pós-infecção. Não houve diferença significativa entre os grupos ao comparar as taxas de positividade do teste de aglutinação microscópica (MAT) (p> 0,05). Oito reações positivas na PCR de urina e fluido vaginal foram detectadas em ambos os grupos, sendo quatro (50%) na urina e quatro (50%) no fluido vaginal, porém sem diferença estatística (p> 0,05). Em ambos os grupos houve maior proporção de amostras positivas para PCR em rins (71,4%) em relação ao útero (28,6%), porém sem diferença significativa (p> 0,05). Não houve diferença significativa entre os grupos ao comparar as taxas de positividade da PCR (p> 0,05). O isolamento de leptospiras da urina e rim de ovelhas mestiças foi possível. Portanto, é possível que ovinos nativos, principalmente os mestiços, tenham relação de resistência com linhagens do sorogrupo Pomona. No entanto, a intensidade e a duração dessa relação precisam ser elucidadas e investigações mais duradouras das infecções naturais e experimentais são necessárias para determinar a natureza epidemiológica dessa relação.Research, Society and Development2021-02-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1237310.33448/rsd-v10i2.12373Research, Society and Development; Vol. 10 No. 2; e19510212373Research, Society and Development; Vol. 10 Núm. 2; e19510212373Research, Society and Development; v. 10 n. 2; e195102123732525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/12373/11135Copyright (c) 2021 Diego Figueiredo da Costa; Davidianne de Andrade Morais; Aline Ferreira da Silva; Ismael Lira Borges; Jefferson da Silva Ferreira; Camila de Sousa Bezerra; Carla Lauise Rodrigues Menezes Pimenta; Maria Luana Cristiny Rodrigues Silva; Sergio Santos de Azevedo; Clebert José Alveshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCosta, Diego Figueiredo da Morais, Davidianne de Andrade Silva, Aline Ferreira da Borges, Ismael Lira Ferreira, Jefferson da Silva Bezerra, Camila de Sousa Pimenta, Carla Lauise Rodrigues Menezes Silva, Maria Luana Cristiny Rodrigues Azevedo, Sergio Santos de Alves, Clebert José 2021-03-02T09:32:39Zoai:ojs.pkp.sfu.ca:article/12373Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:33:58.229482Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed
Dinámica de la infección experimental con Leptospira serogrupo Pomona en diferentes razas de ovinos
Dinâmica da infecção experimental com Leptospira sorogrupo Pomona em diferentes raças de ovinos
title Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed
spellingShingle Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed
Costa, Diego Figueiredo da
Resistance
Leptospirosis
Santa Inês
Crossbred sheep
Pomona.
Resistencia
Leptospirosis
Santa Inês
Oveja mestiza
Pomona.
Resistência
Leptospirose
Santa Inês
Ovelha cruzada
Pomona.
title_short Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed
title_full Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed
title_fullStr Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed
title_full_unstemmed Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed
title_sort Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed
author Costa, Diego Figueiredo da
author_facet Costa, Diego Figueiredo da
Morais, Davidianne de Andrade
Silva, Aline Ferreira da
Borges, Ismael Lira
Ferreira, Jefferson da Silva
Bezerra, Camila de Sousa
Pimenta, Carla Lauise Rodrigues Menezes
Silva, Maria Luana Cristiny Rodrigues
Azevedo, Sergio Santos de
Alves, Clebert José
author_role author
author2 Morais, Davidianne de Andrade
Silva, Aline Ferreira da
Borges, Ismael Lira
Ferreira, Jefferson da Silva
Bezerra, Camila de Sousa
Pimenta, Carla Lauise Rodrigues Menezes
Silva, Maria Luana Cristiny Rodrigues
Azevedo, Sergio Santos de
Alves, Clebert José
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Diego Figueiredo da
Morais, Davidianne de Andrade
Silva, Aline Ferreira da
Borges, Ismael Lira
Ferreira, Jefferson da Silva
Bezerra, Camila de Sousa
Pimenta, Carla Lauise Rodrigues Menezes
Silva, Maria Luana Cristiny Rodrigues
Azevedo, Sergio Santos de
Alves, Clebert José
dc.subject.por.fl_str_mv Resistance
Leptospirosis
Santa Inês
Crossbred sheep
Pomona.
Resistencia
Leptospirosis
Santa Inês
Oveja mestiza
Pomona.
Resistência
Leptospirose
Santa Inês
Ovelha cruzada
Pomona.
topic Resistance
Leptospirosis
Santa Inês
Crossbred sheep
Pomona.
Resistencia
Leptospirosis
Santa Inês
Oveja mestiza
Pomona.
Resistência
Leptospirose
Santa Inês
Ovelha cruzada
Pomona.
description Most of the information about leptospirosis was obtained from experimental infections with rodents, which in spite of being relevant, do not provide all the answers about the disease in animals and human beings, given the variability of interactions which exist between the agent and the different hosts. Therefore, the objective of the present study was to verify the pathogenesis of the infection in native breeds of sheep of Brazil challenged with a strain of the serogroup Pomona.  A total of 10 sheep were divided into two groups with five animals according to the breed. In each group four sheep were challenged by intraperitoneal route with a strain of the serogroup Pomona and one was used as control. Sheep were monitored for 60 days, with collection of blood for serologic diagnosis, as well as vaginal fluid and urine for microbiological and molecular analyses. After this period the animals were submitted to euthanasia and necropsy, with collection of tissues for microbiological, molecular and histopathologic diagnosis. All the challenged sheep presented anti-Leptospira antibodies. Crossbred sheep presented a lower concentration of titers and the antibodies were detected for a shorter period of time when compared to the Santa Inês sheep, with statistical difference in the concentration of the titers on the days 15 (p<0.05), 45 (p<0.05) and 60 (p<0.05) post-infection. There was no significant difference between the groups when comparing the positivity rates of the microscopic agglutination test (MAT) (p>0.05). Eight positive reactions in the urine and vaginal fluid PCR were detected in both groups, being four (50%) in urine and four (50%) in the vaginal fluid, however without statistical difference (p>0.05). In both groups there was a greater proportion of PCR positive samples in kidneys (71.4%) in relation to uterus (28.6%), however without significant difference (p>0.05). There was no significant difference between the groups when comparing the positivity rates of the PCR (p>0.05). The isolation of leptospires from the urine and kidney of a crossbred sheep was possible. Therefore, it is possible that the native sheep, especially the crossbred, may have a relation of resistance with strains of the serogroup Pomona. However the intensity and duration of this relation need to be elucidated and longer-lasting investigations of natural and experimental infections are necessary in order to determine the epidemiological nature of this relation.
publishDate 2021
dc.date.none.fl_str_mv 2021-02-10
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/12373
10.33448/rsd-v10i2.12373
url https://rsdjournal.org/index.php/rsd/article/view/12373
identifier_str_mv 10.33448/rsd-v10i2.12373
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/12373/11135
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 2; e19510212373
Research, Society and Development; Vol. 10 Núm. 2; e19510212373
Research, Society and Development; v. 10 n. 2; e19510212373
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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