Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/12373 |
Resumo: | Most of the information about leptospirosis was obtained from experimental infections with rodents, which in spite of being relevant, do not provide all the answers about the disease in animals and human beings, given the variability of interactions which exist between the agent and the different hosts. Therefore, the objective of the present study was to verify the pathogenesis of the infection in native breeds of sheep of Brazil challenged with a strain of the serogroup Pomona. A total of 10 sheep were divided into two groups with five animals according to the breed. In each group four sheep were challenged by intraperitoneal route with a strain of the serogroup Pomona and one was used as control. Sheep were monitored for 60 days, with collection of blood for serologic diagnosis, as well as vaginal fluid and urine for microbiological and molecular analyses. After this period the animals were submitted to euthanasia and necropsy, with collection of tissues for microbiological, molecular and histopathologic diagnosis. All the challenged sheep presented anti-Leptospira antibodies. Crossbred sheep presented a lower concentration of titers and the antibodies were detected for a shorter period of time when compared to the Santa Inês sheep, with statistical difference in the concentration of the titers on the days 15 (p<0.05), 45 (p<0.05) and 60 (p<0.05) post-infection. There was no significant difference between the groups when comparing the positivity rates of the microscopic agglutination test (MAT) (p>0.05). Eight positive reactions in the urine and vaginal fluid PCR were detected in both groups, being four (50%) in urine and four (50%) in the vaginal fluid, however without statistical difference (p>0.05). In both groups there was a greater proportion of PCR positive samples in kidneys (71.4%) in relation to uterus (28.6%), however without significant difference (p>0.05). There was no significant difference between the groups when comparing the positivity rates of the PCR (p>0.05). The isolation of leptospires from the urine and kidney of a crossbred sheep was possible. Therefore, it is possible that the native sheep, especially the crossbred, may have a relation of resistance with strains of the serogroup Pomona. However the intensity and duration of this relation need to be elucidated and longer-lasting investigations of natural and experimental infections are necessary in order to determine the epidemiological nature of this relation. |
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Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breedDinámica de la infección experimental con Leptospira serogrupo Pomona en diferentes razas de ovinos Dinâmica da infecção experimental com Leptospira sorogrupo Pomona em diferentes raças de ovinosResistanceLeptospirosisSanta InêsCrossbred sheepPomona. ResistenciaLeptospirosisSanta InêsOveja mestizaPomona.ResistênciaLeptospiroseSanta InêsOvelha cruzadaPomona.Most of the information about leptospirosis was obtained from experimental infections with rodents, which in spite of being relevant, do not provide all the answers about the disease in animals and human beings, given the variability of interactions which exist between the agent and the different hosts. Therefore, the objective of the present study was to verify the pathogenesis of the infection in native breeds of sheep of Brazil challenged with a strain of the serogroup Pomona. A total of 10 sheep were divided into two groups with five animals according to the breed. In each group four sheep were challenged by intraperitoneal route with a strain of the serogroup Pomona and one was used as control. Sheep were monitored for 60 days, with collection of blood for serologic diagnosis, as well as vaginal fluid and urine for microbiological and molecular analyses. After this period the animals were submitted to euthanasia and necropsy, with collection of tissues for microbiological, molecular and histopathologic diagnosis. All the challenged sheep presented anti-Leptospira antibodies. Crossbred sheep presented a lower concentration of titers and the antibodies were detected for a shorter period of time when compared to the Santa Inês sheep, with statistical difference in the concentration of the titers on the days 15 (p<0.05), 45 (p<0.05) and 60 (p<0.05) post-infection. There was no significant difference between the groups when comparing the positivity rates of the microscopic agglutination test (MAT) (p>0.05). Eight positive reactions in the urine and vaginal fluid PCR were detected in both groups, being four (50%) in urine and four (50%) in the vaginal fluid, however without statistical difference (p>0.05). In both groups there was a greater proportion of PCR positive samples in kidneys (71.4%) in relation to uterus (28.6%), however without significant difference (p>0.05). There was no significant difference between the groups when comparing the positivity rates of the PCR (p>0.05). The isolation of leptospires from the urine and kidney of a crossbred sheep was possible. Therefore, it is possible that the native sheep, especially the crossbred, may have a relation of resistance with strains of the serogroup Pomona. However the intensity and duration of this relation need to be elucidated and longer-lasting investigations of natural and experimental infections are necessary in order to determine the epidemiological nature of this relation.La mayor parte de la información sobre la leptospirosis se obtuvo de infecciones experimentales con roedores, que a pesar de ser relevantes, no brindan todas las respuestas sobre la enfermedad en animales y seres humanos, dada la variabilidad de interacciones que existen entre el agente y los diferentes hospedadores. Por lo tanto, el objetivo del presente estudio fue verificar la patogénesis de la infección en razas nativas de ovejas de Brasil desafiadas con una cepa del serogrupo Pomona. Un total de 10 ovejas se dividieron en dos grupos con cinco animales según la raza. En cada grupo, cuatro ovejas fueron desafiadas por vía intraperitoneal con una cepa del serogrupo Pomona y una se usó como control. Las ovejas fueron monitoreadas durante 60 días, con recolección de sangre para diagnóstico serológico, así como fluidos vaginales y orina para análisis microbiológicos y moleculares. Luego de este período los animales fueron sometidos a eutanasia y necropsia, con recolección de tejidos para diagnóstico microbiológico, molecular e histopatológico. Todas las ovejas desafiadas presentaron anticuerpos anti-Leptospira. Los ovinos mestizos presentaron menor concentración de títulos y los anticuerpos se detectaron por un período de tiempo más corto en comparación con el ovino Santa Inês, con diferencia estadística en la concentración de los títulos en los días 15 (p <0.05), 45 (p < 0,05) y 60 (p <0,05) post-infección. No hubo diferencia significativa entre los grupos al comparar las tasas de positividad de la prueba de aglutinación microscópica (MAT) (p> 0.05). Se detectaron ocho reacciones positivas en la PCR de orina y fluido vaginal en ambos grupos, siendo cuatro (50%) en orina y cuatro (50%) en fluido vaginal, sin embargo sin diferencia estadística (p> 0.05). En ambos grupos hubo una mayor proporción de muestras positivas a PCR en riñones (71,4%) en relación al útero (28,6%), sin embargo sin diferencia significativa (p> 0,05). No hubo diferencia significativa entre los grupos al comparar las tasas de positividad de la PCR (p> 0.05). Fue posible el aislamiento de leptospiras de la orina y el riñón de una oveja mestiza. Por tanto, es posible que la oveja autóctona brasileña, especialmente la mestiza, pueda tener una relación de resistencia con cepas del serogrupo Pomona. Sin embargo, es necesario dilucidar la intensidad y duración de esta relación y se necesitan investigaciones más duraderas de las infecciones naturales y experimentales para determinar la naturaleza epidemiológica de esta relación.A maioria das informações sobre a leptospirose foi obtida a partir de infecções experimentais com roedores, que apesar de relevantes, não fornecem todas as respostas sobre a doença em animais e seres humanos, dada a variabilidade de interações existentes entre o agente e os diferentes hospedeiros. Portanto, o objetivo do presente estudo foi verificar a patogênese da infecção em raças nativas de ovinos do Brasil desafiados com uma cepa do sorogrupo Pomona. Um total de 10 ovelhas foram divididas em dois grupos com cinco animais de acordo com a raça. Em cada grupo quatro ovelhas foram desafiadas por via intraperitoneal com uma cepa do sorogrupo Pomona e uma foi utilizada como controle. As ovelhas foram acompanhadas por 60 dias, com coleta de sangue para diagnóstico sorológico, bem como de secreção vaginal e urina para análises microbiológicas e moleculares. Após esse período os animais foram submetidos à eutanásia e necropsia, com coleta de tecidos para diagnóstico microbiológico, molecular e histopatológico. Todas as ovelhas desafiadas apresentaram anticorpos anti-Leptospira. Ovinos mestiços apresentaram menor concentração de títulos e os anticorpos foram detectados por menor período de tempo quando comparados aos ovinos Santa Inês, com diferença estatística na concentração dos títulos nos dias 15 (p <0,05), 45 (p < 0,05) e 60 (p <0,05) pós-infecção. Não houve diferença significativa entre os grupos ao comparar as taxas de positividade do teste de aglutinação microscópica (MAT) (p> 0,05). Oito reações positivas na PCR de urina e fluido vaginal foram detectadas em ambos os grupos, sendo quatro (50%) na urina e quatro (50%) no fluido vaginal, porém sem diferença estatística (p> 0,05). Em ambos os grupos houve maior proporção de amostras positivas para PCR em rins (71,4%) em relação ao útero (28,6%), porém sem diferença significativa (p> 0,05). Não houve diferença significativa entre os grupos ao comparar as taxas de positividade da PCR (p> 0,05). O isolamento de leptospiras da urina e rim de ovelhas mestiças foi possível. Portanto, é possível que ovinos nativos, principalmente os mestiços, tenham relação de resistência com linhagens do sorogrupo Pomona. No entanto, a intensidade e a duração dessa relação precisam ser elucidadas e investigações mais duradouras das infecções naturais e experimentais são necessárias para determinar a natureza epidemiológica dessa relação.Research, Society and Development2021-02-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1237310.33448/rsd-v10i2.12373Research, Society and Development; Vol. 10 No. 2; e19510212373Research, Society and Development; Vol. 10 Núm. 2; e19510212373Research, Society and Development; v. 10 n. 2; e195102123732525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/12373/11135Copyright (c) 2021 Diego Figueiredo da Costa; Davidianne de Andrade Morais; Aline Ferreira da Silva; Ismael Lira Borges; Jefferson da Silva Ferreira; Camila de Sousa Bezerra; Carla Lauise Rodrigues Menezes Pimenta; Maria Luana Cristiny Rodrigues Silva; Sergio Santos de Azevedo; Clebert José Alveshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCosta, Diego Figueiredo da Morais, Davidianne de Andrade Silva, Aline Ferreira da Borges, Ismael Lira Ferreira, Jefferson da Silva Bezerra, Camila de Sousa Pimenta, Carla Lauise Rodrigues Menezes Silva, Maria Luana Cristiny Rodrigues Azevedo, Sergio Santos de Alves, Clebert José 2021-03-02T09:32:39Zoai:ojs.pkp.sfu.ca:article/12373Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:33:58.229482Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed Dinámica de la infección experimental con Leptospira serogrupo Pomona en diferentes razas de ovinos Dinâmica da infecção experimental com Leptospira sorogrupo Pomona em diferentes raças de ovinos |
title |
Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed |
spellingShingle |
Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed Costa, Diego Figueiredo da Resistance Leptospirosis Santa Inês Crossbred sheep Pomona. Resistencia Leptospirosis Santa Inês Oveja mestiza Pomona. Resistência Leptospirose Santa Inês Ovelha cruzada Pomona. |
title_short |
Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed |
title_full |
Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed |
title_fullStr |
Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed |
title_full_unstemmed |
Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed |
title_sort |
Dynamics of experimental infection with Leptospira serogroup Pomona in different sheep breed |
author |
Costa, Diego Figueiredo da |
author_facet |
Costa, Diego Figueiredo da Morais, Davidianne de Andrade Silva, Aline Ferreira da Borges, Ismael Lira Ferreira, Jefferson da Silva Bezerra, Camila de Sousa Pimenta, Carla Lauise Rodrigues Menezes Silva, Maria Luana Cristiny Rodrigues Azevedo, Sergio Santos de Alves, Clebert José |
author_role |
author |
author2 |
Morais, Davidianne de Andrade Silva, Aline Ferreira da Borges, Ismael Lira Ferreira, Jefferson da Silva Bezerra, Camila de Sousa Pimenta, Carla Lauise Rodrigues Menezes Silva, Maria Luana Cristiny Rodrigues Azevedo, Sergio Santos de Alves, Clebert José |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Costa, Diego Figueiredo da Morais, Davidianne de Andrade Silva, Aline Ferreira da Borges, Ismael Lira Ferreira, Jefferson da Silva Bezerra, Camila de Sousa Pimenta, Carla Lauise Rodrigues Menezes Silva, Maria Luana Cristiny Rodrigues Azevedo, Sergio Santos de Alves, Clebert José |
dc.subject.por.fl_str_mv |
Resistance Leptospirosis Santa Inês Crossbred sheep Pomona. Resistencia Leptospirosis Santa Inês Oveja mestiza Pomona. Resistência Leptospirose Santa Inês Ovelha cruzada Pomona. |
topic |
Resistance Leptospirosis Santa Inês Crossbred sheep Pomona. Resistencia Leptospirosis Santa Inês Oveja mestiza Pomona. Resistência Leptospirose Santa Inês Ovelha cruzada Pomona. |
description |
Most of the information about leptospirosis was obtained from experimental infections with rodents, which in spite of being relevant, do not provide all the answers about the disease in animals and human beings, given the variability of interactions which exist between the agent and the different hosts. Therefore, the objective of the present study was to verify the pathogenesis of the infection in native breeds of sheep of Brazil challenged with a strain of the serogroup Pomona. A total of 10 sheep were divided into two groups with five animals according to the breed. In each group four sheep were challenged by intraperitoneal route with a strain of the serogroup Pomona and one was used as control. Sheep were monitored for 60 days, with collection of blood for serologic diagnosis, as well as vaginal fluid and urine for microbiological and molecular analyses. After this period the animals were submitted to euthanasia and necropsy, with collection of tissues for microbiological, molecular and histopathologic diagnosis. All the challenged sheep presented anti-Leptospira antibodies. Crossbred sheep presented a lower concentration of titers and the antibodies were detected for a shorter period of time when compared to the Santa Inês sheep, with statistical difference in the concentration of the titers on the days 15 (p<0.05), 45 (p<0.05) and 60 (p<0.05) post-infection. There was no significant difference between the groups when comparing the positivity rates of the microscopic agglutination test (MAT) (p>0.05). Eight positive reactions in the urine and vaginal fluid PCR were detected in both groups, being four (50%) in urine and four (50%) in the vaginal fluid, however without statistical difference (p>0.05). In both groups there was a greater proportion of PCR positive samples in kidneys (71.4%) in relation to uterus (28.6%), however without significant difference (p>0.05). There was no significant difference between the groups when comparing the positivity rates of the PCR (p>0.05). The isolation of leptospires from the urine and kidney of a crossbred sheep was possible. Therefore, it is possible that the native sheep, especially the crossbred, may have a relation of resistance with strains of the serogroup Pomona. However the intensity and duration of this relation need to be elucidated and longer-lasting investigations of natural and experimental infections are necessary in order to determine the epidemiological nature of this relation. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-02-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12373 10.33448/rsd-v10i2.12373 |
url |
https://rsdjournal.org/index.php/rsd/article/view/12373 |
identifier_str_mv |
10.33448/rsd-v10i2.12373 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12373/11135 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 2; e19510212373 Research, Society and Development; Vol. 10 Núm. 2; e19510212373 Research, Society and Development; v. 10 n. 2; e19510212373 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052669665214464 |