Cytotoxicity of an orabase from Libidibia Ferrea

Detalhes bibliográficos
Autor(a) principal: Oliveira, Glauber Palma de
Data de Publicação: 2021
Outros Autores: Gomes, Letícia da Silva Soares, Venâncio, Gisely Naura, Lima, Emerson Silva, Souza, Tatiane Pereira de, Bandeira, Maria Fulgência Costa Lima, Toda, Carina, Conde, Nikeila Chacon de Oliveira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/18713
Resumo: Introduction: Libidibia Ferrea is a plant with proven therapeutic properties for the prevention and treatment of oral diseases. Objective: To evaluate an orabase source of Libidibia ferrea for cytotoxicity. Methodologys: The hemolysis test was performed using the qualitative assessment method of free hemoglobin and the fibroblast cell culture test using the Alamar Blue method. The stem bark extract, base vehicle, its adjuvants and orabase base were tested. Results: No hemolysis test either the stem bark extract or the above and its components did not dissipate hemolysis when visually evaluating the supernatant from each well. In the Alamar Blue test, it did not show toxicity in any of the tested solutions. Conclusion: Based on the results, the stem bark extract and the orabase base of Libidibia ferrea L. were not toxic when tested in red blood cells and fibroblast cell cultures.
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spelling Cytotoxicity of an orabase from Libidibia FerreaCitotoxicidad de una orabasa de Libidibia FerreaCitotoxicidade de uma formulação em orabase de Libidibia FerreaPhytotherapyCytotocivityJucá.FitoterapiaCitotoxicidadJucá.FitoterapiaCitotoxicidadeJucá.Introduction: Libidibia Ferrea is a plant with proven therapeutic properties for the prevention and treatment of oral diseases. Objective: To evaluate an orabase source of Libidibia ferrea for cytotoxicity. Methodologys: The hemolysis test was performed using the qualitative assessment method of free hemoglobin and the fibroblast cell culture test using the Alamar Blue method. The stem bark extract, base vehicle, its adjuvants and orabase base were tested. Results: No hemolysis test either the stem bark extract or the above and its components did not dissipate hemolysis when visually evaluating the supernatant from each well. In the Alamar Blue test, it did not show toxicity in any of the tested solutions. Conclusion: Based on the results, the stem bark extract and the orabase base of Libidibia ferrea L. were not toxic when tested in red blood cells and fibroblast cell cultures.Introducción: Libidibia Ferrea es una planta con probadas propiedades terapéuticas para la prevención y el tratamiento de enfermedades bucodentales. Objetivo: evaluar una fuente de orabasa de Libidibia ferrea para la citotoxicidad. Metodología: La prueba de hemólisis se realizó mediante el método de evaluación cualitativa de hemoglobina libre y la prueba de cultivo celular de fibroblastos mediante el método Alamar Blue. Se ensayaron el extracto de corteza del tallo, el vehículo base, sus adyuvantes y la base de orabasa. Resultados: No se realizó prueba de hemólisis ni el extracto de corteza del tallo ni lo anterior y sus componentes no disiparon la hemólisis al evaluar visualmente el sobrenadante de cada pocillo. En la prueba de Alamar Blue, no mostró toxicidad en ninguna de las soluciones probadas. Conclusión: Según los resultados, el extracto de corteza de tallo y la base de orabasa de Libidibia ferrea L. no fueron tóxicos cuando se probaron en cultivos de glóbulos rojos y fibroblastos.A Libidibia Ferrea é uma planta que possui inúmeras propriedades terapêuticas comprovadas para prevenção e tratamento de afecções bucais. Objetivo: Avaliar uma formulação em orabase de Libidibia ferrea quanto a citotoxicidade. Metodologia: O teste de hemólise foi realizado através do método de avaliação qualitativa de hemoglobina livre e o teste de cultura de células de fibroblastos através do método de Alamar Blue. Foram testados o extrato da casca do caule, veículo da formulação, seus adjuvantes e a formulação orabase. Resultados: No teste de hemólise tanto o extrato da casca do caule quanto a formulação e seus componentes não apresentaram hemólise ao avaliar visualmente o sobrenadante de cada poço.  No teste de Alamar Blue não apresentou toxicidade em nenhuma das soluções testadas. Conclusão: Baseado nos resultados, o extrato da casca do caule e a formulação em orabase de Libidibia ferrea L. não foram tóxicos quando testados em hemácias e cultura de células de fibroblastos.Research, Society and Development2021-08-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1871310.33448/rsd-v10i10.18713Research, Society and Development; Vol. 10 No. 10; e133101018713Research, Society and Development; Vol. 10 Núm. 10; e133101018713Research, Society and Development; v. 10 n. 10; e1331010187132525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/18713/16670Copyright (c) 2021 Glauber Palma de Oliveira; Letícia da Silva Soares Gomes; Gisely Naura Venâncio; Emerson Silva Lima; Tatiane Pereira de Souza; Maria Fulgência Costa Lima Bandeira; Carina Toda; Nikeila Chacon de Oliveira Condehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessOliveira, Glauber Palma de Gomes, Letícia da Silva Soares Venâncio, Gisely Naura Lima, Emerson Silva Souza, Tatiane Pereira de Bandeira, Maria Fulgência Costa Lima Toda, CarinaConde, Nikeila Chacon de Oliveira 2021-10-02T21:49:16Zoai:ojs.pkp.sfu.ca:article/18713Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:38:46.863515Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Cytotoxicity of an orabase from Libidibia Ferrea
Citotoxicidad de una orabasa de Libidibia Ferrea
Citotoxicidade de uma formulação em orabase de Libidibia Ferrea
title Cytotoxicity of an orabase from Libidibia Ferrea
spellingShingle Cytotoxicity of an orabase from Libidibia Ferrea
Oliveira, Glauber Palma de
Phytotherapy
Cytotocivity
Jucá.
Fitoterapia
Citotoxicidad
Jucá.
Fitoterapia
Citotoxicidade
Jucá.
title_short Cytotoxicity of an orabase from Libidibia Ferrea
title_full Cytotoxicity of an orabase from Libidibia Ferrea
title_fullStr Cytotoxicity of an orabase from Libidibia Ferrea
title_full_unstemmed Cytotoxicity of an orabase from Libidibia Ferrea
title_sort Cytotoxicity of an orabase from Libidibia Ferrea
author Oliveira, Glauber Palma de
author_facet Oliveira, Glauber Palma de
Gomes, Letícia da Silva Soares
Venâncio, Gisely Naura
Lima, Emerson Silva
Souza, Tatiane Pereira de
Bandeira, Maria Fulgência Costa Lima
Toda, Carina
Conde, Nikeila Chacon de Oliveira
author_role author
author2 Gomes, Letícia da Silva Soares
Venâncio, Gisely Naura
Lima, Emerson Silva
Souza, Tatiane Pereira de
Bandeira, Maria Fulgência Costa Lima
Toda, Carina
Conde, Nikeila Chacon de Oliveira
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Glauber Palma de
Gomes, Letícia da Silva Soares
Venâncio, Gisely Naura
Lima, Emerson Silva
Souza, Tatiane Pereira de
Bandeira, Maria Fulgência Costa Lima
Toda, Carina
Conde, Nikeila Chacon de Oliveira
dc.subject.por.fl_str_mv Phytotherapy
Cytotocivity
Jucá.
Fitoterapia
Citotoxicidad
Jucá.
Fitoterapia
Citotoxicidade
Jucá.
topic Phytotherapy
Cytotocivity
Jucá.
Fitoterapia
Citotoxicidad
Jucá.
Fitoterapia
Citotoxicidade
Jucá.
description Introduction: Libidibia Ferrea is a plant with proven therapeutic properties for the prevention and treatment of oral diseases. Objective: To evaluate an orabase source of Libidibia ferrea for cytotoxicity. Methodologys: The hemolysis test was performed using the qualitative assessment method of free hemoglobin and the fibroblast cell culture test using the Alamar Blue method. The stem bark extract, base vehicle, its adjuvants and orabase base were tested. Results: No hemolysis test either the stem bark extract or the above and its components did not dissipate hemolysis when visually evaluating the supernatant from each well. In the Alamar Blue test, it did not show toxicity in any of the tested solutions. Conclusion: Based on the results, the stem bark extract and the orabase base of Libidibia ferrea L. were not toxic when tested in red blood cells and fibroblast cell cultures.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-07
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/18713
10.33448/rsd-v10i10.18713
url https://rsdjournal.org/index.php/rsd/article/view/18713
identifier_str_mv 10.33448/rsd-v10i10.18713
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/18713/16670
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 10; e133101018713
Research, Society and Development; Vol. 10 Núm. 10; e133101018713
Research, Society and Development; v. 10 n. 10; e133101018713
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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