Strontium ranelate promotes increased peri-implant bone formation in ovariectomized rats.
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/9092 |
Resumo: | This study aimed to evaluate the systemic effect of strontium ranelate (SR) on peri-implant bone tissue. Thirty-six adult rats were divided into three experimental groups: sham (SHAM), ovariectomized (OVX) and ovariectomized rats treated with strontium ranelate (OVX-Sr). Strontium ranelate (625mg/kg) was administered by oral gavage on a daily basis. The implants were installed on the tibiae. The euthanasia occurred 42 and 60 days after the implants were installed, and the biomechanical (reverse torque); PCR-RT; histological; immunohistochemical; confocal microscopy and histometric analysis were performed. Quantitative data was subjected to statistical tests with significance level set at p<0.05. Significant increase in implant reverse torque in OVX-Sr was observed when compared to OVX. PCR analysis showed an increase in the genetic expression of the proteins responsible for bone formation in OVX-SR. In the histological analysis, SHAM and OVX-Sr showed a higher degree of maturation of peri-implant bone tissue. Ran-Sr presented higher immunolabeling for ALP and OPN proteins when compared to OVX. In the confocal microscopy, OVX-Sr there was good bone neoformation showed by incorporation of Alizarin red fluorochrome. The histometric analysis, bone implant contact (BIC) and neoformed bone area (NBA) presented statistically difference among all groups, and the Ran-Sr presented the highest BIC. Thus, strontium ranelate improves osseointegration and quality of neoformed bone tissue around implants in estrogen deficient rats. |
| id |
UNIFEI_3a4bb7c854add3b13c127a510757ede7 |
|---|---|
| oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/9092 |
| network_acronym_str |
UNIFEI |
| network_name_str |
Research, Society and Development |
| repository_id_str |
|
| spelling |
Strontium ranelate promotes increased peri-implant bone formation in ovariectomized rats.El ranelato de estroncio promueve el aumento de la formación ósea periimplantaria en ratas ovariectomizadas.Ranelato de Estrôncio promove aumento na formação óssea periimplantar em ratas ovariectomizadas.OsteoporosisStrontiumOsseointegrationBone.OsteoporosisEstroncioOsseointegracionHueso.OsteoporoseEstrôncioOsseointegraçãoOsso.This study aimed to evaluate the systemic effect of strontium ranelate (SR) on peri-implant bone tissue. Thirty-six adult rats were divided into three experimental groups: sham (SHAM), ovariectomized (OVX) and ovariectomized rats treated with strontium ranelate (OVX-Sr). Strontium ranelate (625mg/kg) was administered by oral gavage on a daily basis. The implants were installed on the tibiae. The euthanasia occurred 42 and 60 days after the implants were installed, and the biomechanical (reverse torque); PCR-RT; histological; immunohistochemical; confocal microscopy and histometric analysis were performed. Quantitative data was subjected to statistical tests with significance level set at p<0.05. Significant increase in implant reverse torque in OVX-Sr was observed when compared to OVX. PCR analysis showed an increase in the genetic expression of the proteins responsible for bone formation in OVX-SR. In the histological analysis, SHAM and OVX-Sr showed a higher degree of maturation of peri-implant bone tissue. Ran-Sr presented higher immunolabeling for ALP and OPN proteins when compared to OVX. In the confocal microscopy, OVX-Sr there was good bone neoformation showed by incorporation of Alizarin red fluorochrome. The histometric analysis, bone implant contact (BIC) and neoformed bone area (NBA) presented statistically difference among all groups, and the Ran-Sr presented the highest BIC. Thus, strontium ranelate improves osseointegration and quality of neoformed bone tissue around implants in estrogen deficient rats.Este estudio tiene como objetivo evaluar el efecto sistémico del ranelato de estroncio (SR) en el tejido óseo periimplantario. Treinta y seis ratas adultas fueron divididas en tres grupos experimentales: SHAM (SHAM), ovariectomizadas (OVX) y ovariectomizadas tratadas con ranelato de estroncio (OVX-Sr). El ranelato de estroncio (625mg/kg) se administró por vía oral diariamente. Los implantes se instalaron en la tibia. La eutanasia se produjo 42 y 60 días después de la instalación del implante, y se realizaron análisis biomecánicos (torsión inversa); PCR-RT; histológicos; inmunohistoquímicos; microscopía confocal e histométricos. Los datos cuantitativos se sometieron a pruebas estadísticas con un nivel de significación establecido en p<0,05. Se observó un aumento significativo del torque inverso del implante en OVX-Sr en comparación con OVX. El análisis por PCR mostró un aumento en la expresión genética de las proteínas responsables de la formación de huesos en OVX-SR. En el análisis histológico, SHAM y OVX-Sr mostraron un mayor grado de maduración del tejido óseo periimplantario. OVX-Sr mostró una marca de inmunidad más alta para las proteínas ALP y OPN en comparación con OVX. En la microscopía confocal, OVX-Sr hubo una buena neoformación ósea mostrada por la incorporación del fluorocromo rojo de Alizarin. El análisis histórico, el contacto con el implante óseo (BIC) y el área ósea neoformada (NBA) mostraron diferencias estadísticas entre todos los grupos, y Ran-Sr mostró el BIC más alto. Así, el ranelato de estroncio mejora la osteointegración y la calidad del tejido óseo neoformado alrededor de los implantes en ratas con deficiencia de estrógeno.Este estudo objetiva avaliar o efeito sistémico do ranelato de estrôncio (SR) no tecido ósseo peri-implantar. Trinta e seis ratas adultas foram divididas em três grupos experimentais: SHAM (SHAM), ovariectomizadas (OVX) e ovariectomizadas tratadas com ranelato de estrôncio (OVX-Sr). O ranelato de estrôncio (625mg/kg) era administrado por gavagem oral diariamente. Os implantes foram instalados na tíbia. A eutanásia ocorreu 42 e 60 dias após a instalação dos implantes, e a biomecânica (torque reverso); PCR-RT; histológica; imunoistoquímica; microscopia confocal e análise histométrica foram realizadas. Os dados quantitativos foram submetidos a testes estatísticos com nível de significância fixado em p<0,05. Foi observado um aumento significativo do torque reverso do implante em OVX-Sr quando comparado com OVX. A análise por PCR mostrou um aumento na expressão genética das proteínas responsáveis pela formação óssea em OVX-SR. Na análise histológica, SHAM e OVX-Sr mostraram um maior grau de maturação do tecido ósseo periimplantar. OVX-Sr apresentou uma maior imunomarcação para as proteínas ALP e OPN quando comparadas com as OVX. Na microscopia confocal, OVX-Sr houve boa neoformação óssea mostrada pela incorporação de fluorocromo vermelho de Alizarina. A análise histométrica, o contacto do implante ósseo (BIC) e a área óssea neoformada (NBA) apresentaram diferenças estatísticas entre todos os grupos, e o Ran-Sr apresentou o BIC mais elevado. Assim, o ranelato de estrôncio melhora a osseointegração e a qualidade do tecido ósseo neoformado em torno de implantes em ratos deficientes em estrogénio.Research, Society and Development2020-10-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/909210.33448/rsd-v9i10.9092Research, Society and Development; Vol. 9 No. 10; e7539109092Research, Society and Development; Vol. 9 Núm. 10; e7539109092Research, Society and Development; v. 9 n. 10; e75391090922525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/9092/8429Copyright (c) 2020 Fernanda Costa Yogui; Ana Cláudia Ervolino-Silva; Letícia Pitol-Palin; Juliana Zorzi Coléte; Jaqueline Suemi Hassumi; Naara Gabriela Monteiro; Fábio Roberto de Souza Batista; Pedro Henrique Silva Gomes-Ferreira; Roberta Okamotohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessYogui, Fernanda CostaErvolino-Silva, Ana CláudiaPitol-Palin, LetíciaColéte, Juliana ZorziHassumi, Jaqueline SuemiMonteiro, Naara GabrielaBatista, Fábio Roberto de Souza Gomes-Ferreira, Pedro Henrique SilvaOkamoto, Roberta2020-10-31T12:03:23Zoai:ojs.pkp.sfu.ca:article/9092Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:31:25.486846Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Strontium ranelate promotes increased peri-implant bone formation in ovariectomized rats. El ranelato de estroncio promueve el aumento de la formación ósea periimplantaria en ratas ovariectomizadas. Ranelato de Estrôncio promove aumento na formação óssea periimplantar em ratas ovariectomizadas. |
| title |
Strontium ranelate promotes increased peri-implant bone formation in ovariectomized rats. |
| spellingShingle |
Strontium ranelate promotes increased peri-implant bone formation in ovariectomized rats. Yogui, Fernanda Costa Osteoporosis Strontium Osseointegration Bone. Osteoporosis Estroncio Osseointegracion Hueso. Osteoporose Estrôncio Osseointegração Osso. |
| title_short |
Strontium ranelate promotes increased peri-implant bone formation in ovariectomized rats. |
| title_full |
Strontium ranelate promotes increased peri-implant bone formation in ovariectomized rats. |
| title_fullStr |
Strontium ranelate promotes increased peri-implant bone formation in ovariectomized rats. |
| title_full_unstemmed |
Strontium ranelate promotes increased peri-implant bone formation in ovariectomized rats. |
| title_sort |
Strontium ranelate promotes increased peri-implant bone formation in ovariectomized rats. |
| author |
Yogui, Fernanda Costa |
| author_facet |
Yogui, Fernanda Costa Ervolino-Silva, Ana Cláudia Pitol-Palin, Letícia Coléte, Juliana Zorzi Hassumi, Jaqueline Suemi Monteiro, Naara Gabriela Batista, Fábio Roberto de Souza Gomes-Ferreira, Pedro Henrique Silva Okamoto, Roberta |
| author_role |
author |
| author2 |
Ervolino-Silva, Ana Cláudia Pitol-Palin, Letícia Coléte, Juliana Zorzi Hassumi, Jaqueline Suemi Monteiro, Naara Gabriela Batista, Fábio Roberto de Souza Gomes-Ferreira, Pedro Henrique Silva Okamoto, Roberta |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Yogui, Fernanda Costa Ervolino-Silva, Ana Cláudia Pitol-Palin, Letícia Coléte, Juliana Zorzi Hassumi, Jaqueline Suemi Monteiro, Naara Gabriela Batista, Fábio Roberto de Souza Gomes-Ferreira, Pedro Henrique Silva Okamoto, Roberta |
| dc.subject.por.fl_str_mv |
Osteoporosis Strontium Osseointegration Bone. Osteoporosis Estroncio Osseointegracion Hueso. Osteoporose Estrôncio Osseointegração Osso. |
| topic |
Osteoporosis Strontium Osseointegration Bone. Osteoporosis Estroncio Osseointegracion Hueso. Osteoporose Estrôncio Osseointegração Osso. |
| description |
This study aimed to evaluate the systemic effect of strontium ranelate (SR) on peri-implant bone tissue. Thirty-six adult rats were divided into three experimental groups: sham (SHAM), ovariectomized (OVX) and ovariectomized rats treated with strontium ranelate (OVX-Sr). Strontium ranelate (625mg/kg) was administered by oral gavage on a daily basis. The implants were installed on the tibiae. The euthanasia occurred 42 and 60 days after the implants were installed, and the biomechanical (reverse torque); PCR-RT; histological; immunohistochemical; confocal microscopy and histometric analysis were performed. Quantitative data was subjected to statistical tests with significance level set at p<0.05. Significant increase in implant reverse torque in OVX-Sr was observed when compared to OVX. PCR analysis showed an increase in the genetic expression of the proteins responsible for bone formation in OVX-SR. In the histological analysis, SHAM and OVX-Sr showed a higher degree of maturation of peri-implant bone tissue. Ran-Sr presented higher immunolabeling for ALP and OPN proteins when compared to OVX. In the confocal microscopy, OVX-Sr there was good bone neoformation showed by incorporation of Alizarin red fluorochrome. The histometric analysis, bone implant contact (BIC) and neoformed bone area (NBA) presented statistically difference among all groups, and the Ran-Sr presented the highest BIC. Thus, strontium ranelate improves osseointegration and quality of neoformed bone tissue around implants in estrogen deficient rats. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-10-18 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/9092 10.33448/rsd-v9i10.9092 |
| url |
https://rsdjournal.org/index.php/rsd/article/view/9092 |
| identifier_str_mv |
10.33448/rsd-v9i10.9092 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/9092/8429 |
| dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Research, Society and Development |
| publisher.none.fl_str_mv |
Research, Society and Development |
| dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 10; e7539109092 Research, Society and Development; Vol. 9 Núm. 10; e7539109092 Research, Society and Development; v. 9 n. 10; e7539109092 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
| instname_str |
Universidade Federal de Itajubá (UNIFEI) |
| instacron_str |
UNIFEI |
| institution |
UNIFEI |
| reponame_str |
Research, Society and Development |
| collection |
Research, Society and Development |
| repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
| repository.mail.fl_str_mv |
rsd.articles@gmail.com |
| _version_ |
1797052661405581312 |