Biochemical profile and clinical significance of MAPK/KINASE pathway genes in the diagnosis of thyroid neoplasms

Detalhes bibliográficos
Autor(a) principal: Santos, Joyce Nascimento
Data de Publicação: 2022
Outros Autores: Silva, Raissa Monteiro da, Bhattacharjee, Tanmoy Tapobrata, Kulcsar, Marco Aurélio Vamondes, Uno, Miyuki, Chammas, Roger, Canevari, Renata de Azevedo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/26671
Resumo: Thyroid neoplasms are the main types of endocrine malignancy, their incidence increasing in recent years. Routine diagnosis may present inconclusive results, leading to the need for using additional techniques that are more precise, such as molecular analysis. Therefore, it is essential to search for molecular markers for the diagnosis of these malignancies and their different histological types. The objective of this study was to identify diagnostic molecular markers for papillary thyroid carcinomas (PTC) and goiter lesions. For this, expression of genes belonging to the MAPK/KINASE pathway was assessed by the RT-qPCR technique. Additionally, complete biochemical profiles of the samples were obtained using Fourier transform infrared spectroscopy (FTIR). Results of RT-qPCR suggest that FOS, JUN, MAP2K6, CCNA1, SFN genes have the potential to be tumor markers of thyroid lesions, and the MAP2K6, CCNA1, SFN genes further have the potential to distinguish PTC samples from other thyroid lesions. FTIR results showed that PTC lesions can be distinguished from normal and benign tissues with 95.83% efficiency; changes in nucleic acids being the major classifying factor. Overall, results suggest potential of molecular and FTIR analysis in diagnosis of thyroid cancer.
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spelling Biochemical profile and clinical significance of MAPK/KINASE pathway genes in the diagnosis of thyroid neoplasmsPerfil bioquímico y significado clínico de los genes de la vía MAPK /CINASA en el diagnóstico de neoplasias tiroidesPerfil bioquímico e significado clínico de genes da via MAPK/KINASE no diagnóstico de neoplasias de tireoideThyroidGene expressionMolecular markerMAPK/KINASESpectroscopyFTIR.TireoideExpressão gênicaMarcador molecularMAPK/KINASEEspectroscopiaFTIR.TiroidesLa expresion genicaMarcador molecularMAPK/KINASEEspectroscopiaFTIR.Thyroid neoplasms are the main types of endocrine malignancy, their incidence increasing in recent years. Routine diagnosis may present inconclusive results, leading to the need for using additional techniques that are more precise, such as molecular analysis. Therefore, it is essential to search for molecular markers for the diagnosis of these malignancies and their different histological types. The objective of this study was to identify diagnostic molecular markers for papillary thyroid carcinomas (PTC) and goiter lesions. For this, expression of genes belonging to the MAPK/KINASE pathway was assessed by the RT-qPCR technique. Additionally, complete biochemical profiles of the samples were obtained using Fourier transform infrared spectroscopy (FTIR). Results of RT-qPCR suggest that FOS, JUN, MAP2K6, CCNA1, SFN genes have the potential to be tumor markers of thyroid lesions, and the MAP2K6, CCNA1, SFN genes further have the potential to distinguish PTC samples from other thyroid lesions. FTIR results showed that PTC lesions can be distinguished from normal and benign tissues with 95.83% efficiency; changes in nucleic acids being the major classifying factor. Overall, results suggest potential of molecular and FTIR analysis in diagnosis of thyroid cancer.Las neoplasias tiroideas son los principales tipos de malignidad endocrina, incrementándose su incidencia en los últimos años. El diagnóstico de rutina puede presentar resultados no concluyentes, lo que obliga a utilizar técnicas adicionales más precisas, como el análisis molecular. Por lo tanto, es fundamental la búsqueda de marcadores moleculares para el diagnóstico de estas neoplasias malignas y sus diferentes tipos histológicos. El objetivo de este estudio fue identificar marcadores moleculares de diagnóstico para carcinomas papilares de tiroides (CPT) y lesiones de bocio. Para ello, se evaluó la expresión de genes pertenecientes a la vía MAPK/KINASE mediante la técnica RT-qPCR. Además, se obtuvieron perfiles bioquímicos completos de las muestras mediante espectroscopia infrarroja transformada de Fourier (FTIR). Los resultados de RT-qPCR sugieren que los genes FOS, JUN, MAP2K6, CCNA1, SFN tienen el potencial de ser marcadores tumorales de lesiones tiroideas, y los genes MAP2K6, CCNA1, SFN tienen además el potencial de distinguir muestras de CPT de otras lesiones tiroideas. Los resultados de FTIR mostraron que las lesiones de CPT se pueden distinguir de los tejidos normales y benignos con una eficiencia del 95,83 %; siendo los cambios en los ácidos nucleicos el principal factor de clasificación. En general, los resultados sugieren el potencial del análisis molecular y FTIR en el diagnóstico del cáncer de tiroides.As neoplasias da tireoide são os principais tipos de malignidade endócrina, apresentando incidência aumentada nos últimos anos. O diagnóstico de rotina pode apresentar resultados inconclusivos, levando à necessidade de utilização de técnicas adicionais mais precisas, tais como a análise molecular. Portanto, é essencial a busca de marcadores moleculares para o diagnóstico dessas neoplasias e seus diferentes tipos histológicos. O objetivo deste estudo foi identificar marcadores moleculares diagnósticos para carcinoma papilífero de tireoide (CPT) e lesões de bócio. Para isso, a expressão de genes pertencentes à via MAPK/KNASE foi avaliada pela técnica de RT-qPCR. Adicionalmente, perfis bioquímicos completos das amostras foram obtidos por meio da espectroscopia de infravermelho com transformada de Fourier (FTIR). Os resultados do RT-qPCR sugerem que os genes FOS, JUN, MAP2K6, CCNA1, SFN têm o potencial de serem marcadores tumorais de lesões da tireoide, e os genes MAP2K6, CCNA1, SFN têm ainda o potencial de distinguir amostras de CPT de outras lesões de tireoide. Os resultados do FTIR mostraram que as lesões de CPT podem ser diferenciadas de tecidos normais e benignos com 95,83% de eficiência; sendo as alterações nos ácidos nucleicos o principal fator de classificação. No geral, os resultados sugerem o potencial da análise molecular e do FTIR no diagnóstico do câncer de tireoide.Research, Society and Development2022-03-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2667110.33448/rsd-v11i3.26671Research, Society and Development; Vol. 11 No. 3; e58411326671Research, Society and Development; Vol. 11 Núm. 3; e58411326671Research, Society and Development; v. 11 n. 3; e584113266712525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/26671/23594Copyright (c) 2022 Joyce Nascimento Santos; Raissa Monteiro da Silva; Tanmoy Tapobrata Bhattacharjee; Marco Aurélio Vamondes Kulcsar; Miyuki Uno; Roger Chammas; Renata de Azevedo Canevarihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSantos, Joyce NascimentoSilva, Raissa Monteiro daBhattacharjee, Tanmoy TapobrataKulcsar, Marco Aurélio Vamondes Uno, Miyuki Chammas, Roger Canevari, Renata de Azevedo2022-03-09T13:44:38Zoai:ojs.pkp.sfu.ca:article/26671Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:44:39.162020Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Biochemical profile and clinical significance of MAPK/KINASE pathway genes in the diagnosis of thyroid neoplasms
Perfil bioquímico y significado clínico de los genes de la vía MAPK /CINASA en el diagnóstico de neoplasias tiroides
Perfil bioquímico e significado clínico de genes da via MAPK/KINASE no diagnóstico de neoplasias de tireoide
title Biochemical profile and clinical significance of MAPK/KINASE pathway genes in the diagnosis of thyroid neoplasms
spellingShingle Biochemical profile and clinical significance of MAPK/KINASE pathway genes in the diagnosis of thyroid neoplasms
Santos, Joyce Nascimento
Thyroid
Gene expression
Molecular marker
MAPK/KINASE
Spectroscopy
FTIR.
Tireoide
Expressão gênica
Marcador molecular
MAPK/KINASE
Espectroscopia
FTIR.
Tiroides
La expresion genica
Marcador molecular
MAPK/KINASE
Espectroscopia
FTIR.
title_short Biochemical profile and clinical significance of MAPK/KINASE pathway genes in the diagnosis of thyroid neoplasms
title_full Biochemical profile and clinical significance of MAPK/KINASE pathway genes in the diagnosis of thyroid neoplasms
title_fullStr Biochemical profile and clinical significance of MAPK/KINASE pathway genes in the diagnosis of thyroid neoplasms
title_full_unstemmed Biochemical profile and clinical significance of MAPK/KINASE pathway genes in the diagnosis of thyroid neoplasms
title_sort Biochemical profile and clinical significance of MAPK/KINASE pathway genes in the diagnosis of thyroid neoplasms
author Santos, Joyce Nascimento
author_facet Santos, Joyce Nascimento
Silva, Raissa Monteiro da
Bhattacharjee, Tanmoy Tapobrata
Kulcsar, Marco Aurélio Vamondes
Uno, Miyuki
Chammas, Roger
Canevari, Renata de Azevedo
author_role author
author2 Silva, Raissa Monteiro da
Bhattacharjee, Tanmoy Tapobrata
Kulcsar, Marco Aurélio Vamondes
Uno, Miyuki
Chammas, Roger
Canevari, Renata de Azevedo
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Joyce Nascimento
Silva, Raissa Monteiro da
Bhattacharjee, Tanmoy Tapobrata
Kulcsar, Marco Aurélio Vamondes
Uno, Miyuki
Chammas, Roger
Canevari, Renata de Azevedo
dc.subject.por.fl_str_mv Thyroid
Gene expression
Molecular marker
MAPK/KINASE
Spectroscopy
FTIR.
Tireoide
Expressão gênica
Marcador molecular
MAPK/KINASE
Espectroscopia
FTIR.
Tiroides
La expresion genica
Marcador molecular
MAPK/KINASE
Espectroscopia
FTIR.
topic Thyroid
Gene expression
Molecular marker
MAPK/KINASE
Spectroscopy
FTIR.
Tireoide
Expressão gênica
Marcador molecular
MAPK/KINASE
Espectroscopia
FTIR.
Tiroides
La expresion genica
Marcador molecular
MAPK/KINASE
Espectroscopia
FTIR.
description Thyroid neoplasms are the main types of endocrine malignancy, their incidence increasing in recent years. Routine diagnosis may present inconclusive results, leading to the need for using additional techniques that are more precise, such as molecular analysis. Therefore, it is essential to search for molecular markers for the diagnosis of these malignancies and their different histological types. The objective of this study was to identify diagnostic molecular markers for papillary thyroid carcinomas (PTC) and goiter lesions. For this, expression of genes belonging to the MAPK/KINASE pathway was assessed by the RT-qPCR technique. Additionally, complete biochemical profiles of the samples were obtained using Fourier transform infrared spectroscopy (FTIR). Results of RT-qPCR suggest that FOS, JUN, MAP2K6, CCNA1, SFN genes have the potential to be tumor markers of thyroid lesions, and the MAP2K6, CCNA1, SFN genes further have the potential to distinguish PTC samples from other thyroid lesions. FTIR results showed that PTC lesions can be distinguished from normal and benign tissues with 95.83% efficiency; changes in nucleic acids being the major classifying factor. Overall, results suggest potential of molecular and FTIR analysis in diagnosis of thyroid cancer.
publishDate 2022
dc.date.none.fl_str_mv 2022-03-08
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/26671
10.33448/rsd-v11i3.26671
url https://rsdjournal.org/index.php/rsd/article/view/26671
identifier_str_mv 10.33448/rsd-v11i3.26671
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/26671/23594
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 3; e58411326671
Research, Society and Development; Vol. 11 Núm. 3; e58411326671
Research, Society and Development; v. 11 n. 3; e58411326671
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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