Levan production by submerged fermentation using Zymomonas mobilis cct 4494
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/8526 |
Resumo: | Levan is an exopolysaccharide formed by fructose units produced by microorganisms during the fermentation of a culture medium containing sucrose, yeast extract and mineral salts. It is a biopolymer with industrial applications, both in the food area (color and flavor fixative, thickener and stabilizer of various foods) and also in the pharmaceutical (blood plasma substitute, immunomodulator, anticarcinogenic and hypocholesterolemic). This work aimed to specifically address aspects related to the design of levana production from the bacterium Zymomonas mobilis CCT 4494, varying the conditions of a submerged fermentation. The fermentation conditions for the production of levana were evaluated from a factorial planning and the response surface methodology was applied. The results demonstrate that the low p value found for the levan intercept (Y1), indicated that the levels studied in this research were well chosen and that the regression coefficient of the equation described the experiment with above 99% of confidence. Of the independent variables analyzed, agitation and its binary combination with temperature represented the most significant for this biopolymer biosynthesis. The highest production of levan obtained was 10.58 g.L-1, when using the initial sucrose concentration of 150 g.L-1. The values of biopolymer and biomass reduced significantly at temperatures above 30 °C and the fermentation product, such as the growth of Zymomonas mobilis CCT 4494, was proportional to the increase on concentration of sucrose tested. |
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Levan production by submerged fermentation using Zymomonas mobilis cct 4494Producción de levana por fermentación sumergida utilizando Zymomonas mobilis cct 4494Produção de levana por fermentação submersa utilizando Zymomonas mobilis cct 4494BiopolymersPolysaccharidesExopolysaccharidesBacteriaProduction.BiopolímerosPolisacáridosExopolysacáridosBactériasProducción.BiopolímerosPolissacarídeosExopolissacarídeosBactériaProdução.Levan is an exopolysaccharide formed by fructose units produced by microorganisms during the fermentation of a culture medium containing sucrose, yeast extract and mineral salts. It is a biopolymer with industrial applications, both in the food area (color and flavor fixative, thickener and stabilizer of various foods) and also in the pharmaceutical (blood plasma substitute, immunomodulator, anticarcinogenic and hypocholesterolemic). This work aimed to specifically address aspects related to the design of levana production from the bacterium Zymomonas mobilis CCT 4494, varying the conditions of a submerged fermentation. The fermentation conditions for the production of levana were evaluated from a factorial planning and the response surface methodology was applied. The results demonstrate that the low p value found for the levan intercept (Y1), indicated that the levels studied in this research were well chosen and that the regression coefficient of the equation described the experiment with above 99% of confidence. Of the independent variables analyzed, agitation and its binary combination with temperature represented the most significant for this biopolymer biosynthesis. The highest production of levan obtained was 10.58 g.L-1, when using the initial sucrose concentration of 150 g.L-1. The values of biopolymer and biomass reduced significantly at temperatures above 30 °C and the fermentation product, such as the growth of Zymomonas mobilis CCT 4494, was proportional to the increase on concentration of sucrose tested.Levana está formada por unidades de fructosa y producida por microorganismos a partir de la fermentación de un medio de cultivo que contiene sacarosa y sales minerales. Es un biopolímero con aplicaciones industriales utilizadas en el área de alimentos (fijador de color y sabor, espesante y estabilizador) y también en productos farmacéuticos (sustituto del plasma sanguíneo, inmunomodulador, anticarcinogénico e hipocolesterolémico). Este estudio tuvo como objetivo abordar específicamente aspectos relacionados con el diseño de la producción de levana por la bacteria Zymomonas mobilis CCT 4494, variando los parámetros de una fermentación sumergida. Las condiciones de fermentación y producción fueron evaluadas por una planificación factorial y se aplicó la metodología de la superficie de respuesta. Los resultados muestran que el bajo valor p encontrado para la interceptación de levana (Y1) indicó que los niveles estudiados en esta investigación fueron bien elegidos y que el coeficiente de regresión de la ecuación describió el experimento por encima del 99% de confianza. De las variables independientes analizadas, la agitación y su combinación binaria con la temperatura mostraron las mayores diferencias significativas en los resultados. El cultivo de biopolímero más grande fue de 10,58 g.L-1 de levana. Los valores de Levana y biomasa disminuyeron significativamente a temperaturas superiores a 30 ºC y el producto de fermentación, como el crecimiento de Zymomonas mobilis CCT 4494, fue proporcional al aumento de la concentración de sacarosa probada.A levana é formada por unidades de frutose e produzida por micro-organismos a partir da fermentação de um meio de cultura contendo sacarose e sais minerais. É um biopolímero com aplicações industriais usado na área alimentícia (fixador de cores e sabores, espessante e estabilizante) e também na farmacêutica (substituto de plasma sanguíneo, imunomodulador, anticarcinogênico e hipocolesterolêmico). Esse trabalho teve como objetivo abordar especificamente aspectos relacionados ao delineamento da produção de levana pela bactéria Zymomonas mobilis CCT 4494, variando os parâmetros de uma fermentação submersa. As condições para a fermentação e produção foram avaliadas por um planejamento fatorial e foi aplicada a metodologia de superfície de resposta. Os resultados demonstram que o baixo valor de p encontrado para o intercepto levana (Y1), indicou que os níveis estudados nesta pesquisa foram bem escolhidos e que o coeficiente de regressão da equação descreveu o experimento acima de 99% de confiança. Das variáveis independentes analisadas, a agitação e sua combinação binária com a temperatura apresentaram as maiores diferenças significativas nos resultados. O maior cultivo do biopolímero foi de 10,58 g.L-1 de levana. Os valores de levana e da biomassa reduziram significativamente em temperaturas superiores a 30 °C e o produto da fermentação, como o crescimento de Zymomonas mobilis CCT 4494, foram proporcionais ao aumento da concentração de sacarose testada.Research, Society and Development2020-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/852610.33448/rsd-v9i10.8526Research, Society and Development; Vol. 9 No. 10; e3899108526Research, Society and Development; Vol. 9 Núm. 10; e3899108526Research, Society and Development; v. 9 n. 10; e38991085262525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/8526/7725Copyright (c) 2020 Letícia Biazzi Lima; Mariane Daniella Da Silva; Talita Garcia Lopes Viçoso; Maria Laura Ferreira Martins; Jaciara Jesus Silva; Jesley Pires Tomaz Silva; Simone Aparecida Biazzi Lapena; Simone Aparecida Biazzi Lapena; Crispin Humberto Garcia-Cruz; Fernanda Maria Pagane Guereschi Ernandeshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessLima, Letícia BiazziSilva, Mariane Daniella Da Viçoso, Talita Garcia LopesMartins, Maria Laura Ferreira Silva, Jaciara JesusSilva, Jesley Pires TomazLapena, Simone Aparecida BiazziGarcia-Cruz, Crispin Humberto Ernandes, Fernanda Maria Pagane Guereschi2020-10-31T12:03:23Zoai:ojs.pkp.sfu.ca:article/8526Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:30:58.969960Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Levan production by submerged fermentation using Zymomonas mobilis cct 4494 Producción de levana por fermentación sumergida utilizando Zymomonas mobilis cct 4494 Produção de levana por fermentação submersa utilizando Zymomonas mobilis cct 4494 |
| title |
Levan production by submerged fermentation using Zymomonas mobilis cct 4494 |
| spellingShingle |
Levan production by submerged fermentation using Zymomonas mobilis cct 4494 Lima, Letícia Biazzi Biopolymers Polysaccharides Exopolysaccharides Bacteria Production. Biopolímeros Polisacáridos Exopolysacáridos Bactérias Producción. Biopolímeros Polissacarídeos Exopolissacarídeos Bactéria Produção. |
| title_short |
Levan production by submerged fermentation using Zymomonas mobilis cct 4494 |
| title_full |
Levan production by submerged fermentation using Zymomonas mobilis cct 4494 |
| title_fullStr |
Levan production by submerged fermentation using Zymomonas mobilis cct 4494 |
| title_full_unstemmed |
Levan production by submerged fermentation using Zymomonas mobilis cct 4494 |
| title_sort |
Levan production by submerged fermentation using Zymomonas mobilis cct 4494 |
| author |
Lima, Letícia Biazzi |
| author_facet |
Lima, Letícia Biazzi Silva, Mariane Daniella Da Viçoso, Talita Garcia Lopes Martins, Maria Laura Ferreira Silva, Jaciara Jesus Silva, Jesley Pires Tomaz Lapena, Simone Aparecida Biazzi Garcia-Cruz, Crispin Humberto Ernandes, Fernanda Maria Pagane Guereschi |
| author_role |
author |
| author2 |
Silva, Mariane Daniella Da Viçoso, Talita Garcia Lopes Martins, Maria Laura Ferreira Silva, Jaciara Jesus Silva, Jesley Pires Tomaz Lapena, Simone Aparecida Biazzi Garcia-Cruz, Crispin Humberto Ernandes, Fernanda Maria Pagane Guereschi |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lima, Letícia Biazzi Silva, Mariane Daniella Da Viçoso, Talita Garcia Lopes Martins, Maria Laura Ferreira Silva, Jaciara Jesus Silva, Jesley Pires Tomaz Lapena, Simone Aparecida Biazzi Garcia-Cruz, Crispin Humberto Ernandes, Fernanda Maria Pagane Guereschi |
| dc.subject.por.fl_str_mv |
Biopolymers Polysaccharides Exopolysaccharides Bacteria Production. Biopolímeros Polisacáridos Exopolysacáridos Bactérias Producción. Biopolímeros Polissacarídeos Exopolissacarídeos Bactéria Produção. |
| topic |
Biopolymers Polysaccharides Exopolysaccharides Bacteria Production. Biopolímeros Polisacáridos Exopolysacáridos Bactérias Producción. Biopolímeros Polissacarídeos Exopolissacarídeos Bactéria Produção. |
| description |
Levan is an exopolysaccharide formed by fructose units produced by microorganisms during the fermentation of a culture medium containing sucrose, yeast extract and mineral salts. It is a biopolymer with industrial applications, both in the food area (color and flavor fixative, thickener and stabilizer of various foods) and also in the pharmaceutical (blood plasma substitute, immunomodulator, anticarcinogenic and hypocholesterolemic). This work aimed to specifically address aspects related to the design of levana production from the bacterium Zymomonas mobilis CCT 4494, varying the conditions of a submerged fermentation. The fermentation conditions for the production of levana were evaluated from a factorial planning and the response surface methodology was applied. The results demonstrate that the low p value found for the levan intercept (Y1), indicated that the levels studied in this research were well chosen and that the regression coefficient of the equation described the experiment with above 99% of confidence. Of the independent variables analyzed, agitation and its binary combination with temperature represented the most significant for this biopolymer biosynthesis. The highest production of levan obtained was 10.58 g.L-1, when using the initial sucrose concentration of 150 g.L-1. The values of biopolymer and biomass reduced significantly at temperatures above 30 °C and the fermentation product, such as the growth of Zymomonas mobilis CCT 4494, was proportional to the increase on concentration of sucrose tested. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-10-01 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
| status_str |
publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/8526 10.33448/rsd-v9i10.8526 |
| url |
https://rsdjournal.org/index.php/rsd/article/view/8526 |
| identifier_str_mv |
10.33448/rsd-v9i10.8526 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/8526/7725 |
| dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Research, Society and Development |
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Research, Society and Development |
| dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 10; e3899108526 Research, Society and Development; Vol. 9 Núm. 10; e3899108526 Research, Society and Development; v. 9 n. 10; e3899108526 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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UNIFEI |
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UNIFEI |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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1797052659828523008 |