Vitamin D Receptor (VDR) polymorphism and antiproliferative activity of cholecalciferol in cancer cells
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/10810 |
Resumo: | Vitamin D (VD) is a steroid hormone with multiple biological functions in the body and its activity requires the binding to the receptor named VDR. VDR polymorphisms seems to be involved in the development of several types of cancer. Herein we performed the genotyping of two VDR polymorphisms (Fok I and Taq I) in MCF-7 breast cancer and U87-MG glioblastoma (GBM) cell lines and investigated the antiproliferative effect of the VD analog cholecalciferol. Polymorphisms were identified by PCR-RFLP and the effect of VD was determined by viability and clonogenic assays. VD inhibited the growth of both tumor cells in vitro. MCF-7 cells were more sensitive than U87-MG cells at concentrations ranging from 0.1nM to 1000nM. The same primer pairs used for PCR amplification of VDR gene in MCF-7 failed to amplify a fragment of expected size in the U87-MG cell line. VDR Fok I and Taq I polymorphisms in breast cancer MCF-7 cells were characterized as FF (CC) and TT respectively. The absence of amplification of VDR gene fragment in U87-MG suggests a possible chromosomal rearrangement and/or impairment of gene expression of VDR which could interfere in the sensitivity of this cell line to vitamin D. |
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Research, Society and Development |
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Vitamin D Receptor (VDR) polymorphism and antiproliferative activity of cholecalciferol in cancer cellsPolimorfismo del Receptor de Vitamina D (VDR) y actividad antiproliferativa del colecalciferol en células cancerosasPolimorfismo do Receptor de Vitamina D (VDR) e atividade antiproliferativa do colecalciferol em células cancerígenasCâncer de MamaGlioblastomaReceptor de vitamina DPCR-RFLPSNPTumor cerebral.Breast CancerGlioblastomaVitamin D receptorPCR-RFLPSNPBrain cancer.Cáncer de mamaGlioblastomaReceptor de vitamina DPCR-RFLPSNPTumor cerebral.Vitamin D (VD) is a steroid hormone with multiple biological functions in the body and its activity requires the binding to the receptor named VDR. VDR polymorphisms seems to be involved in the development of several types of cancer. Herein we performed the genotyping of two VDR polymorphisms (Fok I and Taq I) in MCF-7 breast cancer and U87-MG glioblastoma (GBM) cell lines and investigated the antiproliferative effect of the VD analog cholecalciferol. Polymorphisms were identified by PCR-RFLP and the effect of VD was determined by viability and clonogenic assays. VD inhibited the growth of both tumor cells in vitro. MCF-7 cells were more sensitive than U87-MG cells at concentrations ranging from 0.1nM to 1000nM. The same primer pairs used for PCR amplification of VDR gene in MCF-7 failed to amplify a fragment of expected size in the U87-MG cell line. VDR Fok I and Taq I polymorphisms in breast cancer MCF-7 cells were characterized as FF (CC) and TT respectively. The absence of amplification of VDR gene fragment in U87-MG suggests a possible chromosomal rearrangement and/or impairment of gene expression of VDR which could interfere in the sensitivity of this cell line to vitamin D.La vitamina D (VD) es una hormona esteroide con múltiples funciones biológicas en el cuerpo y su actividad requiere unirse al receptor llamado VDR. Los polimorfismos de VDR parecen estar involucrados en el desarrollo de varios tipos de cánceres. Aquí, genotipamos dos polimorfismos VDR (Fok I y Taq I) en las líneas celulares de cáncer de mama MCF-7 y glioblastoma U87-MG (GBM) e investigamos el efecto antiproliferativo del análogo de colecalciferol de la VD. Se identificaron polimorfismos. mediante PCR-RFLP y el efecto de VD se determinó mediante ensayos de viabilidad y clonogénicos. VD inhibió el crecimiento de ambas células tumorales in vitro. Las células MCF-7 eran más sensibles que las células U87-MG a concentraciones que variaban de 0,1 nM a 1000 nM. Los mismos pares de iniciadores utilizados para la amplificación por PCR del gen VDR en MCF-7 no lograron amplificar un fragmento del tamaño esperado en la línea celular U87-MG. Los polimorfismos de VDR Fok I y Taq I en células de cáncer de mama MCF-7 se caracterizaron como FF (CC) y TT, respectivamente. La ausencia de amplificación del fragmento del gen VDR en U87-MG sugiere un posible reordenamiento cromosómico y/o expresión del gen VDR alterada que podría interferir con la sensibilidad de esta línea celular a VD.A vitamina D (VD) é um hormônio esteróide com múltiplas funções biológicas no corpo e sua atividade requer a ligação ao receptor denominado VDR. Os polimorfismos do VDR parecem estar envolvidos no desenvolvimento de vários tipos de cânceres. Aqui, realizamos a genotipagem de dois polimorfismos VDR (Fok I e Taq I) em linhagens celulares de câncer de mama MCF-7 e glioblastoma U87-MG (GBM) e investigamos o efeito antiproliferativo do colecalciferol análogo da VD. Os polimorfismos foram identificados por PCR-RFLP e o efeito da VD foi determinado por viabilidade e ensaios clonogênicos. A VD inibiu o crescimento de ambas as células tumorais in vitro. As células MCF-7 foram mais sensíveis do que as células U87-MG em concentrações que variam de 0,1 nM a 1000 nM. Os mesmos pares de primers usados para amplificação por PCR do gene VDR em MCF-7 não conseguiram amplificar um fragmento de tamanho esperado na linha de células U87-MG. Os polimorfismos VDR Fok I e Taq I em células MCF-7 de câncer de mama foram caracterizados como FF (CC) e TT, respectivamente. A ausência de amplificação do fragmento do gene VDR no U87-MG sugere um possível rearranjo cromossômico e/ou prejuízo da expressão gênica do VDR que poderia interferir na sensibilidade dessa linhagem celular à VD.Research, Society and Development2020-12-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1081010.33448/rsd-v9i12.10810Research, Society and Development; Vol. 9 No. 12; e8991210810Research, Society and Development; Vol. 9 Núm. 12; e8991210810Research, Society and Development; v. 9 n. 12; e89912108102525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/10810/9696Copyright (c) 2020 Andressa Rodrigues Lopes; Vitor Gabriel Felipe; Raquel Gouvêa dos Santos; Wagner Gouvêa dos Santoshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessLopes, Andressa Rodrigues Felipe, Vitor GabrielSantos, Raquel Gouvêa dosSantos, Wagner Gouvêa dos2020-12-30T23:32:22Zoai:ojs.pkp.sfu.ca:article/10810Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:32:47.857757Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Vitamin D Receptor (VDR) polymorphism and antiproliferative activity of cholecalciferol in cancer cells Polimorfismo del Receptor de Vitamina D (VDR) y actividad antiproliferativa del colecalciferol en células cancerosas Polimorfismo do Receptor de Vitamina D (VDR) e atividade antiproliferativa do colecalciferol em células cancerígenas |
title |
Vitamin D Receptor (VDR) polymorphism and antiproliferative activity of cholecalciferol in cancer cells |
spellingShingle |
Vitamin D Receptor (VDR) polymorphism and antiproliferative activity of cholecalciferol in cancer cells Lopes, Andressa Rodrigues Câncer de Mama Glioblastoma Receptor de vitamina D PCR-RFLP SNP Tumor cerebral. Breast Cancer Glioblastoma Vitamin D receptor PCR-RFLP SNP Brain cancer. Cáncer de mama Glioblastoma Receptor de vitamina D PCR-RFLP SNP Tumor cerebral. |
title_short |
Vitamin D Receptor (VDR) polymorphism and antiproliferative activity of cholecalciferol in cancer cells |
title_full |
Vitamin D Receptor (VDR) polymorphism and antiproliferative activity of cholecalciferol in cancer cells |
title_fullStr |
Vitamin D Receptor (VDR) polymorphism and antiproliferative activity of cholecalciferol in cancer cells |
title_full_unstemmed |
Vitamin D Receptor (VDR) polymorphism and antiproliferative activity of cholecalciferol in cancer cells |
title_sort |
Vitamin D Receptor (VDR) polymorphism and antiproliferative activity of cholecalciferol in cancer cells |
author |
Lopes, Andressa Rodrigues |
author_facet |
Lopes, Andressa Rodrigues Felipe, Vitor Gabriel Santos, Raquel Gouvêa dos Santos, Wagner Gouvêa dos |
author_role |
author |
author2 |
Felipe, Vitor Gabriel Santos, Raquel Gouvêa dos Santos, Wagner Gouvêa dos |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Lopes, Andressa Rodrigues Felipe, Vitor Gabriel Santos, Raquel Gouvêa dos Santos, Wagner Gouvêa dos |
dc.subject.por.fl_str_mv |
Câncer de Mama Glioblastoma Receptor de vitamina D PCR-RFLP SNP Tumor cerebral. Breast Cancer Glioblastoma Vitamin D receptor PCR-RFLP SNP Brain cancer. Cáncer de mama Glioblastoma Receptor de vitamina D PCR-RFLP SNP Tumor cerebral. |
topic |
Câncer de Mama Glioblastoma Receptor de vitamina D PCR-RFLP SNP Tumor cerebral. Breast Cancer Glioblastoma Vitamin D receptor PCR-RFLP SNP Brain cancer. Cáncer de mama Glioblastoma Receptor de vitamina D PCR-RFLP SNP Tumor cerebral. |
description |
Vitamin D (VD) is a steroid hormone with multiple biological functions in the body and its activity requires the binding to the receptor named VDR. VDR polymorphisms seems to be involved in the development of several types of cancer. Herein we performed the genotyping of two VDR polymorphisms (Fok I and Taq I) in MCF-7 breast cancer and U87-MG glioblastoma (GBM) cell lines and investigated the antiproliferative effect of the VD analog cholecalciferol. Polymorphisms were identified by PCR-RFLP and the effect of VD was determined by viability and clonogenic assays. VD inhibited the growth of both tumor cells in vitro. MCF-7 cells were more sensitive than U87-MG cells at concentrations ranging from 0.1nM to 1000nM. The same primer pairs used for PCR amplification of VDR gene in MCF-7 failed to amplify a fragment of expected size in the U87-MG cell line. VDR Fok I and Taq I polymorphisms in breast cancer MCF-7 cells were characterized as FF (CC) and TT respectively. The absence of amplification of VDR gene fragment in U87-MG suggests a possible chromosomal rearrangement and/or impairment of gene expression of VDR which could interfere in the sensitivity of this cell line to vitamin D. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/10810 10.33448/rsd-v9i12.10810 |
url |
https://rsdjournal.org/index.php/rsd/article/view/10810 |
identifier_str_mv |
10.33448/rsd-v9i12.10810 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/10810/9696 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 12; e8991210810 Research, Society and Development; Vol. 9 Núm. 12; e8991210810 Research, Society and Development; v. 9 n. 12; e8991210810 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052743492304896 |