Phytochemical screening, in vitro antioxidant, photoprotective and hemolytic activities of ethyl acetate extracts of the fruits and branches from Spondias tuberosa (umbu)
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/11825 |
Resumo: | Spondias tuberosa is a medicinal plant used to treat inflammatory conditions and digestive disorders. The phytochemical screening, antioxidant, photoprotective and hemoytic activities of ethyl acetate extracts from the fruits and branches were investigated. Antioxidant activities were evaluated using DPPH, ABTS and phosphomolybdenum assays. The photoprotective and hemolytic activities was evaluated by the spectrophotometric method. Identification of compounds classes from the ethyl acetate branches extract was performed by HPLC. TLC analysis demonstrated presence of flavonoids, cinnamic derivatives, triterpenes and steroids in both extracts, and proanthocyanidins and leucoanthocyanidins only in branches, and it was identified by HPLC gallic acid, chlorogenic acid, caffeic acid, ferulic acid in the ethyl acetate extract of branches. Ethyl acetate branches extracts showed the best results: total phenolic compounds (83.88 ± 0.2 mg GAE/g), flavonoids (11.24 ± 2.0 mg QE/g), DPPH (88.80 ± 0.4 %), ABTS (68.92 ± 0.7%), phosphomolybdenum (27.94 ± 0.26%) and sun protection factor (SPF) with 15.50 ± 0.41. In addition, both extracts did not present hemolytic activity. Therefore, the branches extracts can be used in the production of photoprotective application with phytocosmetics. This work is the first report of photoprotective activity of Spondias tuberosa (umbu). |
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Phytochemical screening, in vitro antioxidant, photoprotective and hemolytic activities of ethyl acetate extracts of the fruits and branches from Spondias tuberosa (umbu) Cribado fitoquímico, actividades antioxidante, fotoprotectora y hemolítica in vitro de extractos de acetato de etilo de frutos y ramas de Spondias tuberosa (umbu)Triagem fitoquímica, atividades antioxidante, fotoprotetora e hemolítica in vitro dos extratos de acetato de etila dos frutos e ramos de Spondias tuberosa (umbu)Spondias tuberosaUmbuFotoproteçãoAtividade hemolíticaAntioxidantes.Spondias tuberosaUmbuFotoprotecciónActividad hemolíticaAntioxidantes. Spondias tuberosaUmbuPhotoprotectionHemolytic activityAntioxidants. Spondias tuberosa is a medicinal plant used to treat inflammatory conditions and digestive disorders. The phytochemical screening, antioxidant, photoprotective and hemoytic activities of ethyl acetate extracts from the fruits and branches were investigated. Antioxidant activities were evaluated using DPPH, ABTS and phosphomolybdenum assays. The photoprotective and hemolytic activities was evaluated by the spectrophotometric method. Identification of compounds classes from the ethyl acetate branches extract was performed by HPLC. TLC analysis demonstrated presence of flavonoids, cinnamic derivatives, triterpenes and steroids in both extracts, and proanthocyanidins and leucoanthocyanidins only in branches, and it was identified by HPLC gallic acid, chlorogenic acid, caffeic acid, ferulic acid in the ethyl acetate extract of branches. Ethyl acetate branches extracts showed the best results: total phenolic compounds (83.88 ± 0.2 mg GAE/g), flavonoids (11.24 ± 2.0 mg QE/g), DPPH (88.80 ± 0.4 %), ABTS (68.92 ± 0.7%), phosphomolybdenum (27.94 ± 0.26%) and sun protection factor (SPF) with 15.50 ± 0.41. In addition, both extracts did not present hemolytic activity. Therefore, the branches extracts can be used in the production of photoprotective application with phytocosmetics. This work is the first report of photoprotective activity of Spondias tuberosa (umbu).Spondias tuberosa es una planta medicinal que se usa para tratar afecciones inflamatorias y trastornos digestivos. Se investigó el cribado fitoquímico, antioxidante, fotoprotector y hemoítico de extractos de acetato de etilo de frutos y ramas. Las actividades antioxidantes se evaluaron mediante ensayos de DPPH, ABTS y fosfomolibdeno. La actividad fotoprotectora y hemolítica se evaluó mediante el método espectrofotométrico. La identificación de clases de compuestos a partir del extracto de ramas de acetato de etilo se realizó mediante HPLC. El análisis de TLC demostró presencia de flavonoides, derivados cinámicos, triterpenos y esteroides en ambos extractos y proantocianidinas y leucoantocianidinas solo en ramas, y se identificó por HPLC ácido gálico, ácido clorogénico, ácido cafeico, ácido ferúlico en el extracto de acetato de etilo de ramas. Los extractos de ramas de acetato de etilo mostraron los mejores resultados: compuestos fenólicos totales (83,88 ± 0,2 mg GAE/g), flavonoides (11,24 ± 2,0 mg QE/g), DPPH (88,80 ± 0,4%), ABTS (68,92 ± 0,7%), fosfomolibdeno (27,94 ± 0,26%) y factor de protección solar (SPF) con 15,50 ± 0,41. Además, ambos extractos no presentaron actividad hemolítica. Por tanto, los extractos de ramas se pueden utilizar en la producción de aplicaciones fotoprotectoras con fitocosméticos. Este trabajo es el primer reporte de actividad fotoprotectora de Spondias tuberosa (umbu).Spondias tuberosa é uma planta medicinal usada para tratar doenças inflamatórias e distúrbios digestivos. Foram investigadas as atividades fitoquímica, antioxidante, fotoprotetora e hemótica dos extratos de acetato de etila dos frutos e ramos. As atividades antioxidantes foram avaliadas pelos ensaios de DPPH, ABTS e fosfomolibdênio. As atividades fotoprotetora e hemolítica foram avaliadas pelo método espectrofotométrico. A identificação das classes de compostos do extrato dos ramos de acetato de etila foi realizada por HPLC. A análise por TLC demonstrou a presença de flavonóides, derivados cinâmicos, triterpenos e esteróides em ambos os extratos, e proantocianidinas e leucoantocianidinas apenas em ramos, e foi identificada por HPLC ácido gálico, ácido clorogênico, ácido caféico, ácido ferúlico no extrato de acetato de etila dos ramos. Extratos de ramos de acetato de etila apresentaram os melhores resultados: compostos fenólicos totais (83,88 ± 0,2 mg GAE/g), flavonóides (11,24 ± 2,0 mg QE/g), DPPH (88,80 ± 0,4%), ABTS (68,92 ± 0,7%), fosfomolibdênio (27,94 ± 0,26%) e fator de proteção solar (FPS) com 15,50 ± 0,41. Além disso, ambos os extratos não apresentaram atividade hemolítica. Portanto, os extratos dos ramos podem ser utilizados na produção de aplicações fotoprotetoras com fitocosméticos. Este trabalho é o primeiro relato da atividade fotoprotetora de Spondias tuberosa (umbu).Research, Society and Development2021-01-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1182510.33448/rsd-v10i1.11825Research, Society and Development; Vol. 10 No. 1; e38610111825Research, Society and Development; Vol. 10 Núm. 1; e38610111825Research, Society and Development; v. 10 n. 1; e386101118252525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/11825/10637Copyright (c) 2021 Amanda Dias de Araújo ; Fernanda Granja da Silva Oliveira; Francinete Francis Lacerda; Carlos Eduardo Sales da Silva; Bruno Souza dos Santos; Clovis Macêdo Bezerra-Filho; Bárbara de Azevedo Ramos; Wolfgang Harand; Nicácio Henrique da Silva; Patrícia Maria Guedes Paiva ; Jackson Roberto Guedes da Silva Almeida; Márcia Vanusa da Silva; Maria Tereza dos Santos Correia https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAraújo , Amanda Dias de Oliveira, Fernanda Granja da Silva Lacerda, Francinete Francis Silva, Carlos Eduardo Sales da Santos, Bruno Souza dos Bezerra-Filho, Clovis Macêdo Ramos, Bárbara de Azevedo Harand, Wolfgang Silva, Nicácio Henrique da Paiva , Patrícia Maria Guedes Almeida, Jackson Roberto Guedes da Silva Silva, Márcia Vanusa da Correia , Maria Tereza dos Santos 2021-02-20T21:19:23Zoai:ojs.pkp.sfu.ca:article/11825Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:33:33.289014Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Phytochemical screening, in vitro antioxidant, photoprotective and hemolytic activities of ethyl acetate extracts of the fruits and branches from Spondias tuberosa (umbu) Cribado fitoquímico, actividades antioxidante, fotoprotectora y hemolítica in vitro de extractos de acetato de etilo de frutos y ramas de Spondias tuberosa (umbu) Triagem fitoquímica, atividades antioxidante, fotoprotetora e hemolítica in vitro dos extratos de acetato de etila dos frutos e ramos de Spondias tuberosa (umbu) |
| title |
Phytochemical screening, in vitro antioxidant, photoprotective and hemolytic activities of ethyl acetate extracts of the fruits and branches from Spondias tuberosa (umbu) |
| spellingShingle |
Phytochemical screening, in vitro antioxidant, photoprotective and hemolytic activities of ethyl acetate extracts of the fruits and branches from Spondias tuberosa (umbu) Araújo , Amanda Dias de Spondias tuberosa Umbu Fotoproteção Atividade hemolítica Antioxidantes. Spondias tuberosa Umbu Fotoprotección Actividad hemolítica Antioxidantes. Spondias tuberosa Umbu Photoprotection Hemolytic activity Antioxidants. |
| title_short |
Phytochemical screening, in vitro antioxidant, photoprotective and hemolytic activities of ethyl acetate extracts of the fruits and branches from Spondias tuberosa (umbu) |
| title_full |
Phytochemical screening, in vitro antioxidant, photoprotective and hemolytic activities of ethyl acetate extracts of the fruits and branches from Spondias tuberosa (umbu) |
| title_fullStr |
Phytochemical screening, in vitro antioxidant, photoprotective and hemolytic activities of ethyl acetate extracts of the fruits and branches from Spondias tuberosa (umbu) |
| title_full_unstemmed |
Phytochemical screening, in vitro antioxidant, photoprotective and hemolytic activities of ethyl acetate extracts of the fruits and branches from Spondias tuberosa (umbu) |
| title_sort |
Phytochemical screening, in vitro antioxidant, photoprotective and hemolytic activities of ethyl acetate extracts of the fruits and branches from Spondias tuberosa (umbu) |
| author |
Araújo , Amanda Dias de |
| author_facet |
Araújo , Amanda Dias de Oliveira, Fernanda Granja da Silva Lacerda, Francinete Francis Silva, Carlos Eduardo Sales da Santos, Bruno Souza dos Bezerra-Filho, Clovis Macêdo Ramos, Bárbara de Azevedo Harand, Wolfgang Silva, Nicácio Henrique da Paiva , Patrícia Maria Guedes Almeida, Jackson Roberto Guedes da Silva Silva, Márcia Vanusa da Correia , Maria Tereza dos Santos |
| author_role |
author |
| author2 |
Oliveira, Fernanda Granja da Silva Lacerda, Francinete Francis Silva, Carlos Eduardo Sales da Santos, Bruno Souza dos Bezerra-Filho, Clovis Macêdo Ramos, Bárbara de Azevedo Harand, Wolfgang Silva, Nicácio Henrique da Paiva , Patrícia Maria Guedes Almeida, Jackson Roberto Guedes da Silva Silva, Márcia Vanusa da Correia , Maria Tereza dos Santos |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Araújo , Amanda Dias de Oliveira, Fernanda Granja da Silva Lacerda, Francinete Francis Silva, Carlos Eduardo Sales da Santos, Bruno Souza dos Bezerra-Filho, Clovis Macêdo Ramos, Bárbara de Azevedo Harand, Wolfgang Silva, Nicácio Henrique da Paiva , Patrícia Maria Guedes Almeida, Jackson Roberto Guedes da Silva Silva, Márcia Vanusa da Correia , Maria Tereza dos Santos |
| dc.subject.por.fl_str_mv |
Spondias tuberosa Umbu Fotoproteção Atividade hemolítica Antioxidantes. Spondias tuberosa Umbu Fotoprotección Actividad hemolítica Antioxidantes. Spondias tuberosa Umbu Photoprotection Hemolytic activity Antioxidants. |
| topic |
Spondias tuberosa Umbu Fotoproteção Atividade hemolítica Antioxidantes. Spondias tuberosa Umbu Fotoprotección Actividad hemolítica Antioxidantes. Spondias tuberosa Umbu Photoprotection Hemolytic activity Antioxidants. |
| description |
Spondias tuberosa is a medicinal plant used to treat inflammatory conditions and digestive disorders. The phytochemical screening, antioxidant, photoprotective and hemoytic activities of ethyl acetate extracts from the fruits and branches were investigated. Antioxidant activities were evaluated using DPPH, ABTS and phosphomolybdenum assays. The photoprotective and hemolytic activities was evaluated by the spectrophotometric method. Identification of compounds classes from the ethyl acetate branches extract was performed by HPLC. TLC analysis demonstrated presence of flavonoids, cinnamic derivatives, triterpenes and steroids in both extracts, and proanthocyanidins and leucoanthocyanidins only in branches, and it was identified by HPLC gallic acid, chlorogenic acid, caffeic acid, ferulic acid in the ethyl acetate extract of branches. Ethyl acetate branches extracts showed the best results: total phenolic compounds (83.88 ± 0.2 mg GAE/g), flavonoids (11.24 ± 2.0 mg QE/g), DPPH (88.80 ± 0.4 %), ABTS (68.92 ± 0.7%), phosphomolybdenum (27.94 ± 0.26%) and sun protection factor (SPF) with 15.50 ± 0.41. In addition, both extracts did not present hemolytic activity. Therefore, the branches extracts can be used in the production of photoprotective application with phytocosmetics. This work is the first report of photoprotective activity of Spondias tuberosa (umbu). |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-01-19 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/11825 10.33448/rsd-v10i1.11825 |
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https://rsdjournal.org/index.php/rsd/article/view/11825 |
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10.33448/rsd-v10i1.11825 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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https://rsdjournal.org/index.php/rsd/article/view/11825/10637 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 10 No. 1; e38610111825 Research, Society and Development; Vol. 10 Núm. 1; e38610111825 Research, Society and Development; v. 10 n. 1; e38610111825 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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Research, Society and Development |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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