Antibiofilm activity of glycolic plant extracts on Klebsiella pneumoniae clinical isolates

• Autor(a) principal: Ramos, Lucas de Paula
• Data de Publicação: 2021
• Outros Autores: Pereira, Thaís Cristine, Oliveira, Marcela dos Santos, Assis, Maria Angélica de Sá, Amaral, Suelen Simões, Figueira, Leandro Wagner, Romangnoli, Amanda, Oliveira, Felipe Eduardo, Santos, Carlos Eduardo Rocha, Santos, João Manoel Theotônio dos, Lima, Patrícia Michelle Nagai de, Oliveira, Luciane Dias de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Research, Society and Development
• Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/20270
• topic: Klebsiella pneumoniae; Antibiofilme; Fitoterapia; Bactéria; Resistência; Microbiologia.; Klebsiella pneumoniae; Antibiofilm; Fitoterapia; Bacteria; Resistencia; Microbiología.; Klebsiella pneumoniae; Anti-biofilm; Phytotherapy; Bacteria; Resistance; Microbiology.

Objective: Evaluate the anti-biofilm activity of Gymnema sylvestre, Hamamelis virginiana, Juglans regia, Persea americana, Pfaffia paniculata, Rosmarinus officinalis, Stryphnodendron barbatiman and Thymus vulgaris extracts in 3 MDR strains and 1 ATCC (4352) of Klebsiella pneumoniae. Methods: First were made a screening with broth microdilution test, protocol M7-A9, according to CLSI. Extracts that presented values for Minimal Microbicidal Concentration (MMC) were selected for biofilms tests on 96 wells plates. Treatments used concentrations of 25 and 50 mg/mL, after biofilms were measured by biomass and MTT tests and statistically analyzed by ANOVA and Tukey test (p<0.05). Results: All extracts showed MIC for all K. pneumoniae strains studied, with values ranging from 12.5-100 mg/mL. Biomass of the strains ATCC and MDR strain (400381) of K. pneumoniae obtained reductions of 37.7 and 44.3% with P. paniculata and R. officinalis extracts. The isolate 386546 obtained a reduction of 29.7% (p<0.05) under R. officinalis action. Conclusion: Among the extracts studied, those that were most effective in the anti-biofilm action were J. regia, P. paniculata and R. officinalis. Therefore, these extracts could act as bactericidal agents against K. pneumoniae MDR.