Antibiofilm activity of glycolic plant extracts on Klebsiella pneumoniae clinical isolates
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/20270 |
Resumo: | Objective: Evaluate the anti-biofilm activity of Gymnema sylvestre, Hamamelis virginiana, Juglans regia, Persea americana, Pfaffia paniculata, Rosmarinus officinalis, Stryphnodendron barbatiman and Thymus vulgaris extracts in 3 MDR strains and 1 ATCC (4352) of Klebsiella pneumoniae. Methods: First were made a screening with broth microdilution test, protocol M7-A9, according to CLSI. Extracts that presented values for Minimal Microbicidal Concentration (MMC) were selected for biofilms tests on 96 wells plates. Treatments used concentrations of 25 and 50 mg/mL, after biofilms were measured by biomass and MTT tests and statistically analyzed by ANOVA and Tukey test (p<0.05). Results: All extracts showed MIC for all K. pneumoniae strains studied, with values ranging from 12.5-100 mg/mL. Biomass of the strains ATCC and MDR strain (400381) of K. pneumoniae obtained reductions of 37.7 and 44.3% with P. paniculata and R. officinalis extracts. The isolate 386546 obtained a reduction of 29.7% (p<0.05) under R. officinalis action. Conclusion: Among the extracts studied, those that were most effective in the anti-biofilm action were J. regia, P. paniculata and R. officinalis. Therefore, these extracts could act as bactericidal agents against K. pneumoniae MDR. |
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Antibiofilm activity of glycolic plant extracts on Klebsiella pneumoniae clinical isolatesActividad antibiofilm de extractos glicólicos de plantas en aislados clínicos de Klebsiella pneumoniaeAtividade antibiofilme de extratos glicólicos de plantas em isolados clínicos de Klebsiella pneumoniaeKlebsiella pneumoniaeAntibiofilmeFitoterapiaBactériaResistênciaMicrobiologia.Klebsiella pneumoniaeAntibiofilmFitoterapiaBacteriaResistenciaMicrobiología.Klebsiella pneumoniaeAnti-biofilmPhytotherapyBacteriaResistanceMicrobiology.Objective: Evaluate the anti-biofilm activity of Gymnema sylvestre, Hamamelis virginiana, Juglans regia, Persea americana, Pfaffia paniculata, Rosmarinus officinalis, Stryphnodendron barbatiman and Thymus vulgaris extracts in 3 MDR strains and 1 ATCC (4352) of Klebsiella pneumoniae. Methods: First were made a screening with broth microdilution test, protocol M7-A9, according to CLSI. Extracts that presented values for Minimal Microbicidal Concentration (MMC) were selected for biofilms tests on 96 wells plates. Treatments used concentrations of 25 and 50 mg/mL, after biofilms were measured by biomass and MTT tests and statistically analyzed by ANOVA and Tukey test (p<0.05). Results: All extracts showed MIC for all K. pneumoniae strains studied, with values ranging from 12.5-100 mg/mL. Biomass of the strains ATCC and MDR strain (400381) of K. pneumoniae obtained reductions of 37.7 and 44.3% with P. paniculata and R. officinalis extracts. The isolate 386546 obtained a reduction of 29.7% (p<0.05) under R. officinalis action. Conclusion: Among the extracts studied, those that were most effective in the anti-biofilm action were J. regia, P. paniculata and R. officinalis. Therefore, these extracts could act as bactericidal agents against K. pneumoniae MDR.Objetivo: Evaluar la actividad anti-biofilm de extractos de Gymnema sylvestre, Hamamelis virginiana, Juglans regia, Persea americana, Pfaffia paniculata, Rosmarinus officinalis, Stryphnodendron barbatiman y Thymus vulgaris en 3 cepas MDR y 1 ATCC (4352) de Klebsiella pneumoniae. Métodos: Primero se realizó un cribado con caldo de prueba de microdilución, protocolo M7-A9, según CLSI. Los extractos que presentaban valores para la concentración microbicida mínima (CMM) se seleccionaron para las pruebas de biopelículas en placas de 96 pocillos. Los tratamientos utilizaron concentraciones de 25 y 50 mg/mL, luego de que se midieran las biopelículas mediante pruebas de biomassa (cristal violeta) y MTT, y se analizaran estadísticamente mediante ANOVA y prueba de Tukey (p<0.05). Resultados: Todos los extractos mostraron CMM para todas las cepas de K. pneumoniae estudiadas, con valores que variaron de 12.5-100 mg/mL. La biomasa de las cepas ATCC y MDR (400381) de K. pneumoniae obtuvo reducciones de 37,7 y 44,3% con extractos de P. paniculata y R. officinalis. El aislado 386546 obtuvo una reducción del 29,7% (p <0,05) bajo la acción de R. officinalis. Conclusión: Entre los extractos estudiados, los más eficaces en la acción anti-biofilm fueron J. regia, P. paniculata y R. officinalis. Por tanto, estos extractos podrían actuar como agentes bactericidas frente a K. pneumoniae MDR.Objetivo: Avaliar a atividade antibiofilme dos extratos de Gymnema sylvestre, Hamamelis virginiana, Juglans regia, Persea americana, Pfaffia paniculata, Rosmarinus officinalis, Stryphnodendron barbatiman e Thymus vulgaris em 3 cepas clínicas e 1 ATCC (4352) de Klebsiella pneumoniae. Métodos: Primeiramente foi feita uma triagem com teste de microdiluição em caldo, protocolo M7-A9, conforme CLSI. Os extratos que apresentaram valores de Concentração Microbicida Mínima (CMM) foram selecionados para testes de biofilmes em placas de 96 poços. Os tratamentos utilizaram concentrações de 25 e 50 mg/mL, após os biofilmes foram mensurados pelos testes de biomassa (Cristal violeta) e MTT, e analisados estatisticamente por ANOVA e teste de Tukey (p <0,05). Resultados: Todos os extratos apresentaram CIM para todas as cepas de K. pneumoniae estudadas, com valores variando de 12,5-100 mg/mL. A biomassa das cepas ATCC e MDR (400381) de K. pneumoniae obteve reduções de 37,7 e 44,3% com os extratos de P. paniculata e R. officinalis. O isolado 386546 obteve redução de 29,7% (p <0,05) sob ação de R. officinalis. Conclusão: Dentre os extratos estudados, os mais eficazes na ação anti-biofilme foram J. regia, P. paniculata e R. officinalis. Portanto, esses extratos poderiam atuar como agentes bactericidas contra K. pneumoniae MDR.Research, Society and Development2021-09-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2027010.33448/rsd-v10i12.20270Research, Society and Development; Vol. 10 No. 12; e240101220270Research, Society and Development; Vol. 10 Núm. 12; e240101220270Research, Society and Development; v. 10 n. 12; e2401012202702525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/20270/18255Copyright (c) 2021 Lucas de Paula Ramos; Thaís Cristine Pereira; Marcela dos Santos Oliveira; Maria Angélica de Sá Assis; Suelen Simões Amaral; Leandro Wagner Figueira; Amanda Romangnoli; Felipe Eduardo Oliveira; Carlos Eduardo Rocha Santos; João Manoel Theotônio dos Santos; Patrícia Michelle Nagai de Lima; Luciane Dias de Oliveirahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRamos, Lucas de Paula Pereira, Thaís CristineOliveira, Marcela dos SantosAssis, Maria Angélica de Sá Amaral, Suelen Simões Figueira, Leandro Wagner Romangnoli, AmandaOliveira, Felipe Eduardo Santos, Carlos Eduardo Rocha Santos, João Manoel Theotônio dos Lima, Patrícia Michelle Nagai deOliveira, Luciane Dias de2021-11-14T20:26:51Zoai:ojs.pkp.sfu.ca:article/20270Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:39:58.381270Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Antibiofilm activity of glycolic plant extracts on Klebsiella pneumoniae clinical isolates Actividad antibiofilm de extractos glicólicos de plantas en aislados clínicos de Klebsiella pneumoniae Atividade antibiofilme de extratos glicólicos de plantas em isolados clínicos de Klebsiella pneumoniae |
title |
Antibiofilm activity of glycolic plant extracts on Klebsiella pneumoniae clinical isolates |
spellingShingle |
Antibiofilm activity of glycolic plant extracts on Klebsiella pneumoniae clinical isolates Ramos, Lucas de Paula Klebsiella pneumoniae Antibiofilme Fitoterapia Bactéria Resistência Microbiologia. Klebsiella pneumoniae Antibiofilm Fitoterapia Bacteria Resistencia Microbiología. Klebsiella pneumoniae Anti-biofilm Phytotherapy Bacteria Resistance Microbiology. |
title_short |
Antibiofilm activity of glycolic plant extracts on Klebsiella pneumoniae clinical isolates |
title_full |
Antibiofilm activity of glycolic plant extracts on Klebsiella pneumoniae clinical isolates |
title_fullStr |
Antibiofilm activity of glycolic plant extracts on Klebsiella pneumoniae clinical isolates |
title_full_unstemmed |
Antibiofilm activity of glycolic plant extracts on Klebsiella pneumoniae clinical isolates |
title_sort |
Antibiofilm activity of glycolic plant extracts on Klebsiella pneumoniae clinical isolates |
author |
Ramos, Lucas de Paula |
author_facet |
Ramos, Lucas de Paula Pereira, Thaís Cristine Oliveira, Marcela dos Santos Assis, Maria Angélica de Sá Amaral, Suelen Simões Figueira, Leandro Wagner Romangnoli, Amanda Oliveira, Felipe Eduardo Santos, Carlos Eduardo Rocha Santos, João Manoel Theotônio dos Lima, Patrícia Michelle Nagai de Oliveira, Luciane Dias de |
author_role |
author |
author2 |
Pereira, Thaís Cristine Oliveira, Marcela dos Santos Assis, Maria Angélica de Sá Amaral, Suelen Simões Figueira, Leandro Wagner Romangnoli, Amanda Oliveira, Felipe Eduardo Santos, Carlos Eduardo Rocha Santos, João Manoel Theotônio dos Lima, Patrícia Michelle Nagai de Oliveira, Luciane Dias de |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ramos, Lucas de Paula Pereira, Thaís Cristine Oliveira, Marcela dos Santos Assis, Maria Angélica de Sá Amaral, Suelen Simões Figueira, Leandro Wagner Romangnoli, Amanda Oliveira, Felipe Eduardo Santos, Carlos Eduardo Rocha Santos, João Manoel Theotônio dos Lima, Patrícia Michelle Nagai de Oliveira, Luciane Dias de |
dc.subject.por.fl_str_mv |
Klebsiella pneumoniae Antibiofilme Fitoterapia Bactéria Resistência Microbiologia. Klebsiella pneumoniae Antibiofilm Fitoterapia Bacteria Resistencia Microbiología. Klebsiella pneumoniae Anti-biofilm Phytotherapy Bacteria Resistance Microbiology. |
topic |
Klebsiella pneumoniae Antibiofilme Fitoterapia Bactéria Resistência Microbiologia. Klebsiella pneumoniae Antibiofilm Fitoterapia Bacteria Resistencia Microbiología. Klebsiella pneumoniae Anti-biofilm Phytotherapy Bacteria Resistance Microbiology. |
description |
Objective: Evaluate the anti-biofilm activity of Gymnema sylvestre, Hamamelis virginiana, Juglans regia, Persea americana, Pfaffia paniculata, Rosmarinus officinalis, Stryphnodendron barbatiman and Thymus vulgaris extracts in 3 MDR strains and 1 ATCC (4352) of Klebsiella pneumoniae. Methods: First were made a screening with broth microdilution test, protocol M7-A9, according to CLSI. Extracts that presented values for Minimal Microbicidal Concentration (MMC) were selected for biofilms tests on 96 wells plates. Treatments used concentrations of 25 and 50 mg/mL, after biofilms were measured by biomass and MTT tests and statistically analyzed by ANOVA and Tukey test (p<0.05). Results: All extracts showed MIC for all K. pneumoniae strains studied, with values ranging from 12.5-100 mg/mL. Biomass of the strains ATCC and MDR strain (400381) of K. pneumoniae obtained reductions of 37.7 and 44.3% with P. paniculata and R. officinalis extracts. The isolate 386546 obtained a reduction of 29.7% (p<0.05) under R. officinalis action. Conclusion: Among the extracts studied, those that were most effective in the anti-biofilm action were J. regia, P. paniculata and R. officinalis. Therefore, these extracts could act as bactericidal agents against K. pneumoniae MDR. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/20270 10.33448/rsd-v10i12.20270 |
url |
https://rsdjournal.org/index.php/rsd/article/view/20270 |
identifier_str_mv |
10.33448/rsd-v10i12.20270 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/20270/18255 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 12; e240101220270 Research, Society and Development; Vol. 10 Núm. 12; e240101220270 Research, Society and Development; v. 10 n. 12; e240101220270 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052689842962432 |