Phenylalanine ammonia lyase (PAL) activity in genotypes of Opuntia spp. against artificial infestation of Dactylopius opuntiae submitted to biotic and abiotic resistance inducers
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/15106 |
Resumo: | Products of biotic and abiotic origin are being investigated for use in pest management, many of these substances can act as resistance inducers, which induces the metabolism of enzymes involved in defense mechanisms. There is a search for efficient and alternative ways of controlling carmine cochineal (Dactylopius opuntiae Cockerell), the main pest of forage palm (Opuntia spp) in Northeast Brazil. The aim of the study was to evaluate the application of biotic and abiotic inducers on the palms to induce resistance through the activity of the antioxidant enzyme phenylalanine ammonia lyase. The experiment was carried out in a greenhouse at the Federal University of Agreste of Pernambuco, using an Completely Randomized Design (CRD), distributed in a factorial scheme (3x8x3) with three Opuntia genotypes, eight treatments: Trichoderma viride URM 6824, Trichoderma viride URM 6823, Aureobasidium pullulans URM 6874, salicylic acid, chitosan, amino butyric acid, distilled water with the insect (positive control), distilled water without the insect (negative control), with three collection periods and four repetitions. The results were subjected to analysis of variance (ANOVA), the means being compared by the Scott-Knott test at the level of 5% probability (p <0.05) and by orthogonal contrasts. The biological inducers T. viride URM 6824 and T. viride URM 6823 as well amino butyric acid influenced the increase in the enzymatic activity of phenylalanine ammonia lyase. This result demonstrates the potential for use in alternative management, and future studies are needed to deepen the applicability in field conditions. |
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Phenylalanine ammonia lyase (PAL) activity in genotypes of Opuntia spp. against artificial infestation of Dactylopius opuntiae submitted to biotic and abiotic resistance inducersActividad de fenilalanina amoniaco liasa (FAL) en Opuntia spp. contra la infestación artificial de Dactylopius opuntiae sometida a inductores de resistencia biótica y abióticaAtividade da fenilalanina amônia-liase (FAL) em genótipos de Opuntia spp. frente a infestação artificial de Dactylopius opuntiae submetidas a indutores de resistência bióticos e abióticosCarmine cochinealOxidative enzymesInfestationForage palm.Cochinilla carmínEnzimas oxidativasInfestaciónPalma forrajera.Cochonilha do carmimEnzimas oxidativasInfestaçãoPalma forrageira.Products of biotic and abiotic origin are being investigated for use in pest management, many of these substances can act as resistance inducers, which induces the metabolism of enzymes involved in defense mechanisms. There is a search for efficient and alternative ways of controlling carmine cochineal (Dactylopius opuntiae Cockerell), the main pest of forage palm (Opuntia spp) in Northeast Brazil. The aim of the study was to evaluate the application of biotic and abiotic inducers on the palms to induce resistance through the activity of the antioxidant enzyme phenylalanine ammonia lyase. The experiment was carried out in a greenhouse at the Federal University of Agreste of Pernambuco, using an Completely Randomized Design (CRD), distributed in a factorial scheme (3x8x3) with three Opuntia genotypes, eight treatments: Trichoderma viride URM 6824, Trichoderma viride URM 6823, Aureobasidium pullulans URM 6874, salicylic acid, chitosan, amino butyric acid, distilled water with the insect (positive control), distilled water without the insect (negative control), with three collection periods and four repetitions. The results were subjected to analysis of variance (ANOVA), the means being compared by the Scott-Knott test at the level of 5% probability (p <0.05) and by orthogonal contrasts. The biological inducers T. viride URM 6824 and T. viride URM 6823 as well amino butyric acid influenced the increase in the enzymatic activity of phenylalanine ammonia lyase. This result demonstrates the potential for use in alternative management, and future studies are needed to deepen the applicability in field conditions.Se están investigando productos de origen biótico y abiótico para su uso en el manejo de plagas, muchas de estas sustancias pueden actuar como inductores de resistencia, lo que induce el metabolismo de enzimas involucradas en los mecanismos de defensa. Se buscan formas eficientes y alternativas de control de la cochinilla carmín (Dactylopius opuntiae Cockerell), principal plaga de la palma forrajera (Opuntia spp) en el noreste de Brasil. El objetivo del estudio fue evaluar la aplicación de inductores bióticos y abióticos en las palmas para inducir resistencia a través de la actividad de la enzima antioxidante fenilalanina amoniaco liasa (FAL). El experimento se llevó a cabo en un invernadero de la Universidad Federal de Agreste de Pernambuco, utilizando un Diseño Completamente Aleatorio (DCA), distribuido en un esquema factorial (3x8x3) con tres genotipos de Opuntia, ocho tratamientos: Trichoderma viride URM 6824, Trichoderma viride URM 6823, Aureobasidium pullulans URM 6874, ácido salicílico, quitosano, ácido amino butírico, agua destilada con el insecto (control positivo), agua destilada sin el insecto (control negativo), con tres períodos de recolección y cuatro repeticiones. Los resultados se sometieron a análisis de varianza (ANOVA), comparándose las medias mediante la prueba de Scott-Knott al nivel de 5% de probabilidad (p <0,05) y mediante contrastes ortogonales. Los inductores biológicos T. viride URM 6824 y T. viride URM 6823 así como ácido amino butírico influyeron en el aumento de la actividad enzimática de FAL. Este resultado demuestra el potencial de uso en manejo alternativo, y se necesitan estudios futuros para profundizar la aplicabilidad en condiciones de campo.Produtos de origem biótica e abiótica estão sendo investigados para utilização no manejo de pragas, muitas destas substâncias podem atuar como indutores de resistência, o que induz a metabolização de enzimas envolvidas nos mecanismos de defesa. Há uma busca por formas eficientes e alternativas de controle da cochonilha do carmim (Dactylopius opuntiae Cockerell) principal praga da palma forrageira (Opuntia spp) no Nordeste do Brasil. O objetivo do estudo foi avaliar a aplicação de indutores bióticos e abióticos em palmas na indução de resistência através da atividade da enzima antioxidante fenilalanina amônia liase. O experimento foi conduzido em casa de vegetação na Universidade Federal do Agreste de Pernambuco, utilizando-se um Delineamento Inteiramente Casualizado (DIC), distribuído em esquema fatorial (3x8x3) sendo três genótipos de Opuntia, oito tratamentos: Trichoderma viride URM 6824, Trichoderma viride URM 6823, Aureobasidium pullulans URM 6874, ácido salicílico, quitosana, ácido amino butírico (BABA), água destilada com o inseto (controle positivo), água destilada sem o inseto (controle negativo), com três períodos de coleta e quatro repetições. Os resultados foram submetidos a análise de variância (ANOVA), sendo as médias comparadas pelo teste de Scott-Knott ao nível de 5% de probabilidade (p< 0,05) e por contrastes ortogonais. Os indutores biológicos T. viride URM 6824 e T. viride URM 6823 assim como o BABA influenciaram no aumento da atividade enzimática da fenilalanina amônia liase. Resultado demonstra a potencialidade de utilização no manejo alternativo, cabendo estudos futuros para aprofundamento sobre aplicabilidade em condições de campo.Research, Society and Development2021-05-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1510610.33448/rsd-v10i5.15106Research, Society and Development; Vol. 10 No. 5; e41610515106Research, Society and Development; Vol. 10 Núm. 5; e41610515106Research, Society and Development; v. 10 n. 5; e416105151062525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/15106/13546Copyright (c) 2021 Raquel Maria da Silva; Edcleyton José de Lima; Maria Alane Pereira Barbosa; Wellington Leal dos Santos; Edson Flávio Teixeira da Silva; Cesar Auguste Badji; Cristina Maria de Souza-Motta; João Tiago Correia Oliveira; Keila Aparecida Moreirahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Raquel Maria da Lima, Edcleyton José de Barbosa, Maria Alane Pereira Santos, Wellington Leal dos Silva, Edson Flávio Teixeira da Badji, Cesar Auguste Souza-Motta, Cristina Maria de Oliveira, João Tiago Correia Moreira, Keila Aparecida 2021-05-17T18:20:49Zoai:ojs.pkp.sfu.ca:article/15106Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:36:01.040139Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Phenylalanine ammonia lyase (PAL) activity in genotypes of Opuntia spp. against artificial infestation of Dactylopius opuntiae submitted to biotic and abiotic resistance inducers Actividad de fenilalanina amoniaco liasa (FAL) en Opuntia spp. contra la infestación artificial de Dactylopius opuntiae sometida a inductores de resistencia biótica y abiótica Atividade da fenilalanina amônia-liase (FAL) em genótipos de Opuntia spp. frente a infestação artificial de Dactylopius opuntiae submetidas a indutores de resistência bióticos e abióticos |
title |
Phenylalanine ammonia lyase (PAL) activity in genotypes of Opuntia spp. against artificial infestation of Dactylopius opuntiae submitted to biotic and abiotic resistance inducers |
spellingShingle |
Phenylalanine ammonia lyase (PAL) activity in genotypes of Opuntia spp. against artificial infestation of Dactylopius opuntiae submitted to biotic and abiotic resistance inducers Silva, Raquel Maria da Carmine cochineal Oxidative enzymes Infestation Forage palm. Cochinilla carmín Enzimas oxidativas Infestación Palma forrajera. Cochonilha do carmim Enzimas oxidativas Infestação Palma forrageira. |
title_short |
Phenylalanine ammonia lyase (PAL) activity in genotypes of Opuntia spp. against artificial infestation of Dactylopius opuntiae submitted to biotic and abiotic resistance inducers |
title_full |
Phenylalanine ammonia lyase (PAL) activity in genotypes of Opuntia spp. against artificial infestation of Dactylopius opuntiae submitted to biotic and abiotic resistance inducers |
title_fullStr |
Phenylalanine ammonia lyase (PAL) activity in genotypes of Opuntia spp. against artificial infestation of Dactylopius opuntiae submitted to biotic and abiotic resistance inducers |
title_full_unstemmed |
Phenylalanine ammonia lyase (PAL) activity in genotypes of Opuntia spp. against artificial infestation of Dactylopius opuntiae submitted to biotic and abiotic resistance inducers |
title_sort |
Phenylalanine ammonia lyase (PAL) activity in genotypes of Opuntia spp. against artificial infestation of Dactylopius opuntiae submitted to biotic and abiotic resistance inducers |
author |
Silva, Raquel Maria da |
author_facet |
Silva, Raquel Maria da Lima, Edcleyton José de Barbosa, Maria Alane Pereira Santos, Wellington Leal dos Silva, Edson Flávio Teixeira da Badji, Cesar Auguste Souza-Motta, Cristina Maria de Oliveira, João Tiago Correia Moreira, Keila Aparecida |
author_role |
author |
author2 |
Lima, Edcleyton José de Barbosa, Maria Alane Pereira Santos, Wellington Leal dos Silva, Edson Flávio Teixeira da Badji, Cesar Auguste Souza-Motta, Cristina Maria de Oliveira, João Tiago Correia Moreira, Keila Aparecida |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Raquel Maria da Lima, Edcleyton José de Barbosa, Maria Alane Pereira Santos, Wellington Leal dos Silva, Edson Flávio Teixeira da Badji, Cesar Auguste Souza-Motta, Cristina Maria de Oliveira, João Tiago Correia Moreira, Keila Aparecida |
dc.subject.por.fl_str_mv |
Carmine cochineal Oxidative enzymes Infestation Forage palm. Cochinilla carmín Enzimas oxidativas Infestación Palma forrajera. Cochonilha do carmim Enzimas oxidativas Infestação Palma forrageira. |
topic |
Carmine cochineal Oxidative enzymes Infestation Forage palm. Cochinilla carmín Enzimas oxidativas Infestación Palma forrajera. Cochonilha do carmim Enzimas oxidativas Infestação Palma forrageira. |
description |
Products of biotic and abiotic origin are being investigated for use in pest management, many of these substances can act as resistance inducers, which induces the metabolism of enzymes involved in defense mechanisms. There is a search for efficient and alternative ways of controlling carmine cochineal (Dactylopius opuntiae Cockerell), the main pest of forage palm (Opuntia spp) in Northeast Brazil. The aim of the study was to evaluate the application of biotic and abiotic inducers on the palms to induce resistance through the activity of the antioxidant enzyme phenylalanine ammonia lyase. The experiment was carried out in a greenhouse at the Federal University of Agreste of Pernambuco, using an Completely Randomized Design (CRD), distributed in a factorial scheme (3x8x3) with three Opuntia genotypes, eight treatments: Trichoderma viride URM 6824, Trichoderma viride URM 6823, Aureobasidium pullulans URM 6874, salicylic acid, chitosan, amino butyric acid, distilled water with the insect (positive control), distilled water without the insect (negative control), with three collection periods and four repetitions. The results were subjected to analysis of variance (ANOVA), the means being compared by the Scott-Knott test at the level of 5% probability (p <0.05) and by orthogonal contrasts. The biological inducers T. viride URM 6824 and T. viride URM 6823 as well amino butyric acid influenced the increase in the enzymatic activity of phenylalanine ammonia lyase. This result demonstrates the potential for use in alternative management, and future studies are needed to deepen the applicability in field conditions. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-11 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/15106 10.33448/rsd-v10i5.15106 |
url |
https://rsdjournal.org/index.php/rsd/article/view/15106 |
identifier_str_mv |
10.33448/rsd-v10i5.15106 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/15106/13546 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 5; e41610515106 Research, Society and Development; Vol. 10 Núm. 5; e41610515106 Research, Society and Development; v. 10 n. 5; e41610515106 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052785446879232 |