Potential therapy with the inhibitor of TGF-β receptors LY2109761 for oral squamous cell carcinoma

Detalhes bibliográficos
Autor(a) principal: Rezende, Arthur Silva
Data de Publicação: 2021
Outros Autores: Carneiro, Anna Cecília Dias Maciel, Silva, Bruna Raphaela Oliveira, Carboni, Simone de Sales Costa Moreira, Crema, Virginia Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/18396
Resumo: One way of trying to control oral squamous cell carcinoma is to invest in new therapies focused on the molecular biology of receptors and their intracellular signaling pathways. This study aimed to evaluate the effect of LY2109761 (an inhibitor of TGF-β receptors) on cell migration in oral squamous cell carcinoma in vitro. Actin cytoskeleton of SCC-4 cells control and LY2109761 (1, 5 and 10 μM) treated on three-dimensional Matrigel were analysed by using confocal laser microscopy. Control and LY2109761 (1, 5 and 10 μM) treated cells that migrated through the membrane of three-dimensional cell migration assays were counted, significance was p<0.05. Control cells were seen with voluminous cytoplasm, cell cortex preserved and actin cytoskeleton well developed with well distributed actin filaments. Regardless of concentration, cells treated showed: rounded morphology and small size, scanty cytoplasm, cortical F-actin less clear that the control cells, and disruption of actin filaments. The migratory cells were inhibited by treatment with LY2109761 [F (3, 11) = 3742, p<0.0001], in a dose-dependent manner. These results suggest that LY2109761 exerts an inhibitory effect on the actin cytoskeleton and cell migration on SCC-4 cells, therefore, it is a promising therapeutic option for oral squamous cell carcinoma.
id UNIFEI_ce93c3171aa1d66d022b3f50fe482b8f
oai_identifier_str oai:ojs.pkp.sfu.ca:article/18396
network_acronym_str UNIFEI
network_name_str Research, Society and Development
repository_id_str
spelling Potential therapy with the inhibitor of TGF-β receptors LY2109761 for oral squamous cell carcinomaTerapia potencial con el inhibidor de los receptores de TGF-β LY2109761 para el carcinoma oral de células escamosasTerapia potencial com o inibidor dos receptores TGF-β LY2109761 para carcinoma de células escamosas oralMigración celularCitoesqueletoLY2109761Carcinoma oral de células escamosasSCC-4.Migração celularCitoesqueletoLY2109761Carcinoma de células escamosas oralSCC-4.Cell migrationCytoskeletonLY2109761Oral squamous cell carcinomaSCC-4.One way of trying to control oral squamous cell carcinoma is to invest in new therapies focused on the molecular biology of receptors and their intracellular signaling pathways. This study aimed to evaluate the effect of LY2109761 (an inhibitor of TGF-β receptors) on cell migration in oral squamous cell carcinoma in vitro. Actin cytoskeleton of SCC-4 cells control and LY2109761 (1, 5 and 10 μM) treated on three-dimensional Matrigel were analysed by using confocal laser microscopy. Control and LY2109761 (1, 5 and 10 μM) treated cells that migrated through the membrane of three-dimensional cell migration assays were counted, significance was p<0.05. Control cells were seen with voluminous cytoplasm, cell cortex preserved and actin cytoskeleton well developed with well distributed actin filaments. Regardless of concentration, cells treated showed: rounded morphology and small size, scanty cytoplasm, cortical F-actin less clear that the control cells, and disruption of actin filaments. The migratory cells were inhibited by treatment with LY2109761 [F (3, 11) = 3742, p<0.0001], in a dose-dependent manner. These results suggest that LY2109761 exerts an inhibitory effect on the actin cytoskeleton and cell migration on SCC-4 cells, therefore, it is a promising therapeutic option for oral squamous cell carcinoma.Una forma de intentar controlar el carcinoma oral de células escamosas es invertir en nuevas terapias centradas en la biología molecular de los receptores y sus vías de señalización intracelular. Este estudio tuvo como objetivo evaluar el efecto de LY2109761 (un inhibidor de los receptores de TGF-β) sobre la migración celular en el carcinoma oral de células escamosas in vitro. El citoesqueleto de actina del control de células SCC-4 y LY2109761 (1, 5 y 10 µM) tratados en Matrigel tridimensional se analizaron utilizando microscopía láser confocal. Se contaron las células de control y tratadas con LY2109761 (1, 5 y 10 µM) que migraron a través de la membrana de los ensayos de migración celular tridimensional, la significancia fue p <0,05. Se observaron células de control con citoplasma voluminoso, corteza celular conservada y citoesqueleto de actina bien desarrollado con filamentos de actina bien distribuidos. Independientemente de la concentración, las células tratadas mostraron: morfología redondeada y tamaño pequeño, citoplasma escaso, F-actina cortical menos clara que las células de control y rotura de los filamentos de actina. Las células migratorias se inhibieron mediante el tratamiento con LY2109761 [F (3, 11) = 3742, p <0,0001], de una manera dependiente de la dosis. Estos resultados sugieren que LY2109761 ejerce un efecto inhibidor sobre el citoesqueleto de actina y la migración celular en las células SCC-4, por lo tanto, es una opción terapéutica prometedora para el carcinoma oral de células escamosas.Uma forma de tentar controlar o carcinoma de células escamosas oral é investir em novas terapias voltadas para a biologia molecular dos receptores e suas vias de sinalização intracelular. Este estudo teve como objetivo avaliar o efeito do LY2109761 (um inibidor dos receptores TGF-β) na migração celular no carcinoma epidermóide oral in vitro. Citoesqueleto de actina de controle de células SCC-4 e LY2109761 (1, 5 e 10 μM) tratado em Matrigel tridimensional foram analisados ​​usando microscopia confocal a laser. Controle e células tratadas com LY2109761 (1, 5 e 10 μM) que migraram através da membrana de ensaios de migração de células tridimensionais foram contadas, a significância foi p <0,05. Células controle foram observadas com citoplasma volumoso, córtex celular preservado e citoesqueleto de actina bem desenvolvido com filamentos de actina bem distribuídos. Independentemente da concentração, as células tratadas apresentaram: morfologia arredondada e tamanho pequeno, citoplasma escasso, F-actina cortical menos clara que as células de controle e rompimento dos filamentos de actina. As células migratórias foram inibidas pelo tratamento com LY2109761 [F (3, 11) = 3742, p <0,0001], de uma forma dependente da dose. Esses resultados sugerem que o LY2109761 exerce um efeito inibitório sobre o citoesqueleto de actina e a migração celular nas células SCC-4, portanto, é uma opção terapêutica promissora para o carcinoma de células escamosas oral.Research, Society and Development2021-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1839610.33448/rsd-v10i9.18396Research, Society and Development; Vol. 10 No. 9; e54810918396Research, Society and Development; Vol. 10 Núm. 9; e54810918396Research, Society and Development; v. 10 n. 9; e548109183962525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/18396/16409Copyright (c) 2021 Arthur Silva Rezende; Anna Cecília Dias Maciel Carneiro; Bruna Raphaela Oliveira Silva; Simone de Sales Costa Moreira Carboni; Virginia Oliveira Cremahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRezende, Arthur SilvaCarneiro, Anna Cecília Dias Maciel Silva, Bruna Raphaela Oliveira Carboni, Simone de Sales Costa Moreira Crema, Virginia Oliveira2021-09-12T14:28:06Zoai:ojs.pkp.sfu.ca:article/18396Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:38:32.895519Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Potential therapy with the inhibitor of TGF-β receptors LY2109761 for oral squamous cell carcinoma
Terapia potencial con el inhibidor de los receptores de TGF-β LY2109761 para el carcinoma oral de células escamosas
Terapia potencial com o inibidor dos receptores TGF-β LY2109761 para carcinoma de células escamosas oral
title Potential therapy with the inhibitor of TGF-β receptors LY2109761 for oral squamous cell carcinoma
spellingShingle Potential therapy with the inhibitor of TGF-β receptors LY2109761 for oral squamous cell carcinoma
Rezende, Arthur Silva
Migración celular
Citoesqueleto
LY2109761
Carcinoma oral de células escamosas
SCC-4.
Migração celular
Citoesqueleto
LY2109761
Carcinoma de células escamosas oral
SCC-4.
Cell migration
Cytoskeleton
LY2109761
Oral squamous cell carcinoma
SCC-4.
title_short Potential therapy with the inhibitor of TGF-β receptors LY2109761 for oral squamous cell carcinoma
title_full Potential therapy with the inhibitor of TGF-β receptors LY2109761 for oral squamous cell carcinoma
title_fullStr Potential therapy with the inhibitor of TGF-β receptors LY2109761 for oral squamous cell carcinoma
title_full_unstemmed Potential therapy with the inhibitor of TGF-β receptors LY2109761 for oral squamous cell carcinoma
title_sort Potential therapy with the inhibitor of TGF-β receptors LY2109761 for oral squamous cell carcinoma
author Rezende, Arthur Silva
author_facet Rezende, Arthur Silva
Carneiro, Anna Cecília Dias Maciel
Silva, Bruna Raphaela Oliveira
Carboni, Simone de Sales Costa Moreira
Crema, Virginia Oliveira
author_role author
author2 Carneiro, Anna Cecília Dias Maciel
Silva, Bruna Raphaela Oliveira
Carboni, Simone de Sales Costa Moreira
Crema, Virginia Oliveira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rezende, Arthur Silva
Carneiro, Anna Cecília Dias Maciel
Silva, Bruna Raphaela Oliveira
Carboni, Simone de Sales Costa Moreira
Crema, Virginia Oliveira
dc.subject.por.fl_str_mv Migración celular
Citoesqueleto
LY2109761
Carcinoma oral de células escamosas
SCC-4.
Migração celular
Citoesqueleto
LY2109761
Carcinoma de células escamosas oral
SCC-4.
Cell migration
Cytoskeleton
LY2109761
Oral squamous cell carcinoma
SCC-4.
topic Migración celular
Citoesqueleto
LY2109761
Carcinoma oral de células escamosas
SCC-4.
Migração celular
Citoesqueleto
LY2109761
Carcinoma de células escamosas oral
SCC-4.
Cell migration
Cytoskeleton
LY2109761
Oral squamous cell carcinoma
SCC-4.
description One way of trying to control oral squamous cell carcinoma is to invest in new therapies focused on the molecular biology of receptors and their intracellular signaling pathways. This study aimed to evaluate the effect of LY2109761 (an inhibitor of TGF-β receptors) on cell migration in oral squamous cell carcinoma in vitro. Actin cytoskeleton of SCC-4 cells control and LY2109761 (1, 5 and 10 μM) treated on three-dimensional Matrigel were analysed by using confocal laser microscopy. Control and LY2109761 (1, 5 and 10 μM) treated cells that migrated through the membrane of three-dimensional cell migration assays were counted, significance was p<0.05. Control cells were seen with voluminous cytoplasm, cell cortex preserved and actin cytoskeleton well developed with well distributed actin filaments. Regardless of concentration, cells treated showed: rounded morphology and small size, scanty cytoplasm, cortical F-actin less clear that the control cells, and disruption of actin filaments. The migratory cells were inhibited by treatment with LY2109761 [F (3, 11) = 3742, p<0.0001], in a dose-dependent manner. These results suggest that LY2109761 exerts an inhibitory effect on the actin cytoskeleton and cell migration on SCC-4 cells, therefore, it is a promising therapeutic option for oral squamous cell carcinoma.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/18396
10.33448/rsd-v10i9.18396
url https://rsdjournal.org/index.php/rsd/article/view/18396
identifier_str_mv 10.33448/rsd-v10i9.18396
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/18396/16409
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 9; e54810918396
Research, Society and Development; Vol. 10 Núm. 9; e54810918396
Research, Society and Development; v. 10 n. 9; e54810918396
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052753004986368

Registros relacionados