The genotypic profile of milk proteins in Holstein cows raised in South of Brazil

Detalhes bibliográficos
Autor(a) principal: Pilonetto, Fabrício
Data de Publicação: 2022
Outros Autores: Coelho Ladeira, Giovanni, Salvian, Mayara, Zampar, Aline, Cucco, Diego de Córdova
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/31530
Resumo: Genotyping in dairy cattle allows knowing the alleles and genotypes associated with milk proteins that affect yield and milk quality. This study aimed to verify the genotype profile for κ-casein I and II (κ-CN), α-s1-casein (α-CN) and β-lactoglobulin (β-LG), for Holstein cows raised in southern Brazil, where there is no scientific evidence on the presence of these alleles in dairy herds. Hair follicles samples from each animal were used for DNA extraction and genotyping. We calculated the allelic and genotypic frequencies for four types of milk protein variants in the population, and a chi-square test was employed to see whether the population of animals used was in Hardy-Weinberg equilibrium. Allele A and AA genotype showed the highest frequency for both κ-CN (56.77 to 80.36%). For α-s1-CN and β-LG, the B allele was the most frequent, as BB (80.26%) and AB (47.15%), respectively. Due to the presence of alleles associated with protein levels in milk, we concluded that these herd cows present milk technological properties interesting to produce dairy products.
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spelling The genotypic profile of milk proteins in Holstein cows raised in South of BrazilEl perfil genotípico de las proteínas de la leche en vacas Holstein criadas en el Sur de BrasilPerfil genotípico de proteínas do leite em vacas da raça Holandês criadas no Sul do Brasil Frequência alélicaCaseínaLactoglobulinaProdução de leite.Frecuencia alélicaCaseínaLactoglobulinaProducción de leche.Allele frequencyCaseinLactoglobulinMilk yield.Genotyping in dairy cattle allows knowing the alleles and genotypes associated with milk proteins that affect yield and milk quality. This study aimed to verify the genotype profile for κ-casein I and II (κ-CN), α-s1-casein (α-CN) and β-lactoglobulin (β-LG), for Holstein cows raised in southern Brazil, where there is no scientific evidence on the presence of these alleles in dairy herds. Hair follicles samples from each animal were used for DNA extraction and genotyping. We calculated the allelic and genotypic frequencies for four types of milk protein variants in the population, and a chi-square test was employed to see whether the population of animals used was in Hardy-Weinberg equilibrium. Allele A and AA genotype showed the highest frequency for both κ-CN (56.77 to 80.36%). For α-s1-CN and β-LG, the B allele was the most frequent, as BB (80.26%) and AB (47.15%), respectively. Due to the presence of alleles associated with protein levels in milk, we concluded that these herd cows present milk technological properties interesting to produce dairy products.El genotipado en bovinos lecheros permite conocer los alelos y genotipos asociados a las proteínas de la leche que afectan el rendimiento y la calidad de la leche. Este estudio tuvo como objetivo verificar el perfil genotípico de κ-caseína I y II (κ-CN), α-s1-caseína (α-CN) y β-lactoglobulina (β-LG), para vacas Holstein criadas en el sur de Brasil, donde no hay evidencia científica sobre la presencia de estos alelos en hatos lecheros. Se utilizaron muestras de folículos pilosos de cada animal para la extracción de ADN y el genotipado. Calculamos las frecuencias alélicas y genotípicas para cuatro tipos de variantes de proteína de leche en la población, y se empleó una prueba de chi-cuadrado para ver si la población de animales utilizada estaba en equilibrio de Hardy-Weinberg. El alelo A y el genotipo AA mostraron la mayor frecuencia para ambos κ-CN (56,77 a 80,36%). Para α-s1-CN y β-LG, el alelo B fue el más frecuente, al igual que BB (80,26%) y AB (47,15%), respectivamente. Debido a la presencia de alelos asociados a los niveles de proteína en la leche, concluimos que estas vacas del hato presentan propiedades tecnológicas de la leche interesantes para la elaboración de productos lácteos.A genotipagem em bovinos leiteiros permite conhecer os alelos e genótipos associados às proteínas que afetam a qualidade e a produção de leite. Este estudo teve como objetivo verificar o perfil genotípico para κ-caseína I e II (κ-CN), α-s1-caseína (α-CN) e β-lactoglobulina (β-LG), para vacas Holandesas criadas no sul do Brasil, onde não há evidências científicas sobre a presença desses alelos em rebanhos leiteiros. Amostras de folículos pilosos de cada animal foram usadas para extração de DNA e genotipagem. Calculamos as frequências alélicas e genotípicas para quatro tipos de variantes de proteína do leite na população, e um teste qui-quadrado foi empregado para verificar se a população de animais utilizada estava em equilíbrio de Hardy-Weinberg. O alelo A e o genótipo AA apresentaram a maior frequência para ambos os κ-CN (56,77 a 80,36%). Para α-s1-CN e β-LG, o alelo B foi o mais frequente, assim como BB (80,26%) e AB (47,15%), respectivamente. Devido à presença de alelos associados aos níveis de proteína no leite, concluímos que essas vacas de rebanho apresentam propriedades tecnológicas do leite interessantes para a produção de produtos lácteos.Research, Society and Development2022-07-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3153010.33448/rsd-v11i9.31530Research, Society and Development; Vol. 11 No. 9; e2911931530Research, Society and Development; Vol. 11 Núm. 9; e2911931530Research, Society and Development; v. 11 n. 9; e29119315302525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/31530/26889Copyright (c) 2022 Fabrício Pilonetto; Giovanni Coelho Ladeira; Mayara Salvian; Aline Zampar; Diego de Córdova Cuccohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessPilonetto, FabrícioCoelho Ladeira, GiovanniSalvian, MayaraZampar, AlineCucco, Diego de Córdova2022-07-21T12:36:16Zoai:ojs.pkp.sfu.ca:article/31530Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:47:50.473845Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv The genotypic profile of milk proteins in Holstein cows raised in South of Brazil
El perfil genotípico de las proteínas de la leche en vacas Holstein criadas en el Sur de Brasil
Perfil genotípico de proteínas do leite em vacas da raça Holandês criadas no Sul do Brasil
title The genotypic profile of milk proteins in Holstein cows raised in South of Brazil
spellingShingle The genotypic profile of milk proteins in Holstein cows raised in South of Brazil
Pilonetto, Fabrício
Frequência alélica
Caseína
Lactoglobulina
Produção de leite.
Frecuencia alélica
Caseína
Lactoglobulina
Producción de leche.
Allele frequency
Casein
Lactoglobulin
Milk yield.
title_short The genotypic profile of milk proteins in Holstein cows raised in South of Brazil
title_full The genotypic profile of milk proteins in Holstein cows raised in South of Brazil
title_fullStr The genotypic profile of milk proteins in Holstein cows raised in South of Brazil
title_full_unstemmed The genotypic profile of milk proteins in Holstein cows raised in South of Brazil
title_sort The genotypic profile of milk proteins in Holstein cows raised in South of Brazil
author Pilonetto, Fabrício
author_facet Pilonetto, Fabrício
Coelho Ladeira, Giovanni
Salvian, Mayara
Zampar, Aline
Cucco, Diego de Córdova
author_role author
author2 Coelho Ladeira, Giovanni
Salvian, Mayara
Zampar, Aline
Cucco, Diego de Córdova
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Pilonetto, Fabrício
Coelho Ladeira, Giovanni
Salvian, Mayara
Zampar, Aline
Cucco, Diego de Córdova
dc.subject.por.fl_str_mv Frequência alélica
Caseína
Lactoglobulina
Produção de leite.
Frecuencia alélica
Caseína
Lactoglobulina
Producción de leche.
Allele frequency
Casein
Lactoglobulin
Milk yield.
topic Frequência alélica
Caseína
Lactoglobulina
Produção de leite.
Frecuencia alélica
Caseína
Lactoglobulina
Producción de leche.
Allele frequency
Casein
Lactoglobulin
Milk yield.
description Genotyping in dairy cattle allows knowing the alleles and genotypes associated with milk proteins that affect yield and milk quality. This study aimed to verify the genotype profile for κ-casein I and II (κ-CN), α-s1-casein (α-CN) and β-lactoglobulin (β-LG), for Holstein cows raised in southern Brazil, where there is no scientific evidence on the presence of these alleles in dairy herds. Hair follicles samples from each animal were used for DNA extraction and genotyping. We calculated the allelic and genotypic frequencies for four types of milk protein variants in the population, and a chi-square test was employed to see whether the population of animals used was in Hardy-Weinberg equilibrium. Allele A and AA genotype showed the highest frequency for both κ-CN (56.77 to 80.36%). For α-s1-CN and β-LG, the B allele was the most frequent, as BB (80.26%) and AB (47.15%), respectively. Due to the presence of alleles associated with protein levels in milk, we concluded that these herd cows present milk technological properties interesting to produce dairy products.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-02
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/31530
10.33448/rsd-v11i9.31530
url https://rsdjournal.org/index.php/rsd/article/view/31530
identifier_str_mv 10.33448/rsd-v11i9.31530
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/31530/26889
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 9; e2911931530
Research, Society and Development; Vol. 11 Núm. 9; e2911931530
Research, Society and Development; v. 11 n. 9; e2911931530
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052716313214976

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