Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Epidemiologia e Controle de Infecção |
Texto Completo: | https://online.unisc.br/seer/index.php/epidemiologia/article/view/5468 |
Resumo: | Background and Objective: Klebsiella pneumoniae is a Gram negative bacilli causing infections related to health care. In recent years, it has become a multiressistente microorganism, because of the production spectrum beta-lactamase enzymes extended and carbapenemases. Therefore, the aim of this study was to evaluate the susceptibility profile of K. pneumoniae in relation to carbapenems; identifying carbapenemase production and to determine the susceptibility profile of the isolates compared to other β -lactams. Methods: This is research on naexperimental basis with quantitative and qualitative approach, which used 27 isolates from different clinical specimens ofpatients whit infection, assigned by reference laboratories. Prior to susceptibility testing, the saples were re-isolated on MacConckey Agar culture meddium and the plates were incubated with semeios at 37° C for 24 hours. Susceptibility testing to carbapenems and other beta-lactams were performed according to the method of Kirby-Bauer. The antibiotic disks used in the tests were amoxicillin - clavulanate, cefoxitin, cefotaxime, ceftazidime, cefepime, imipenem, meropenem and ertapenem. The test for the detection of the enzyme carbapenemase was performed by the use of antibiotics: meropenem, and ertapenem, by Modified Hodge test. Results:All isolates were susceptible to beta-lactam group of carbapenems and no production of carbapenemases, but 18.5 % produced extend-spectrum beta- lactamase extended spectrum and 40.7 % of the strains were resistant to other beta-lactams. Conclusion: The production of beta- lactamase extended spectrum was one of the main factors involved in resistance to antibiotics tested , but other resistance mechanisms were not identified possibly involved. KEY WORDS: Klebsiella pneumoniae. Disease Susceptibility. Beta-lactams. |
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Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactamsAvaliação da suscetibilidade da Klebsiella pneumoniae aos betalactâmicos.Background and Objective: Klebsiella pneumoniae is a Gram negative bacilli causing infections related to health care. In recent years, it has become a multiressistente microorganism, because of the production spectrum beta-lactamase enzymes extended and carbapenemases. Therefore, the aim of this study was to evaluate the susceptibility profile of K. pneumoniae in relation to carbapenems; identifying carbapenemase production and to determine the susceptibility profile of the isolates compared to other β -lactams. Methods: This is research on naexperimental basis with quantitative and qualitative approach, which used 27 isolates from different clinical specimens ofpatients whit infection, assigned by reference laboratories. Prior to susceptibility testing, the saples were re-isolated on MacConckey Agar culture meddium and the plates were incubated with semeios at 37° C for 24 hours. Susceptibility testing to carbapenems and other beta-lactams were performed according to the method of Kirby-Bauer. The antibiotic disks used in the tests were amoxicillin - clavulanate, cefoxitin, cefotaxime, ceftazidime, cefepime, imipenem, meropenem and ertapenem. The test for the detection of the enzyme carbapenemase was performed by the use of antibiotics: meropenem, and ertapenem, by Modified Hodge test. Results:All isolates were susceptible to beta-lactam group of carbapenems and no production of carbapenemases, but 18.5 % produced extend-spectrum beta- lactamase extended spectrum and 40.7 % of the strains were resistant to other beta-lactams. Conclusion: The production of beta- lactamase extended spectrum was one of the main factors involved in resistance to antibiotics tested , but other resistance mechanisms were not identified possibly involved. KEY WORDS: Klebsiella pneumoniae. Disease Susceptibility. Beta-lactams.Justificativa e objetivos: A Klebsiellapneumoniae é um bacilo Gram negativo causador de infecções relacionadas à assistência à saúde. Nos últimos anos, tem se tornando um microrganismo multirresistente, em virtude da produção de enzimas beta-lactamases de espectro estendido e carbapenemases. O estudo teve como objetivos avaliar o perfil de suscetibilidade da K. pneumoniae em relação acarbapenêmicos; identificar produção de carbapenemases e determinar o perfil de suscetibilidade das cepas bacterianas em relação a outros β-lactâmicos. Métodos: Pesquisa com caráter experimental com abordagem quantiqualitativa. Utilizou-se 27 isolados, oriundos de diferentes espécimes clínicos de pacientes com vários tipos de infecção. Os testes de suscetibilidade aos beta-lactâmicos foram realizados pelo método de disco-difusão, seguindo o parâmetro de Kirby-Bauer. Nos testes de suscetibilidade foramutilizados discos de antibióticos, amoxicilina-clavulanato, cefoxitina, cefotaxima, ceftazidima, cefepime, imipenem, meropenem e ertapenem. Para a detecção de carbapenemase, mediante a utilização do teste de Hodge modificado foram utilizados os antibióticos meropenem, e ertapenem. Resultados: Entre ascepas de K. pneumoniae, utilizadas na pesquisa, 59,3% apresentaram suscetibilidade e 40,7% foram resistentes aos beta-lactâmicos, pertencentes ao grupo carbapenêmicos. Não foramidentificadas cepas produtoras de carbapenemases. 18,5% das cepas de K. pneumoniaeforam identificadas como produtoras de β-lactamase de espectro estendido. Conclusão: Com base nos resultados obtidos, a resistência que 18,5% das cepas de K. pneumoniae apresentaram em relação aos β-lactâmicosfoi em função da produção de β-lactamases de espectro estendido, haja visto, que o teste de identificação para essas enzimas foi realizado. Sugere-se mais pesquisas na identificação de cepas produtoras de carbapenemases, dado a grande ocorrência desses microrganimos em isolados clínicos de pacientes com infecção. DESCRITORES: Klebsiella pneumoniae. Susceptibilidade. Beta-lactâmicos.Unisc2015-04-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://online.unisc.br/seer/index.php/epidemiologia/article/view/546810.17058/reci.v5i2.5468Revista de Epidemiologia e Controle de Infecção; Vol. 5 No. 2 (2015); 79-83Revista de Epidemiologia e Controle de Infecção; v. 5 n. 2 (2015); 79-832238-3360reponame:Revista de Epidemiologia e Controle de Infecçãoinstname:Universidade de Santa Cruz do Sul (UNISC)instacron:UNISCporhttps://online.unisc.br/seer/index.php/epidemiologia/article/view/5468/4294Coelho, Isabelle CarvalhoSilva, Francisco Laurindo daNunes, Maria do Rosário Conceição MouraLopes, Liliana SilvaCarneiro, Leandro PessoaFerreira, Pedro Henrique Piauilino Benvindoinfo:eu-repo/semantics/openAccess2019-01-21T12:55:14Zoai:ojs.online.unisc.br:article/5468Revistahttps://online.unisc.br/seer/index.php/epidemiologia/indexONGhttp://online.unisc.br/seer/index.php/epidemiologia/oai||liapossuelo@unisc.br|| julia.kern@hotmail.com||reci.unisc@gmail.com2238-33602238-3360opendoar:2019-01-21T12:55:14Revista de Epidemiologia e Controle de Infecção - Universidade de Santa Cruz do Sul (UNISC)false |
dc.title.none.fl_str_mv |
Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams Avaliação da suscetibilidade da Klebsiella pneumoniae aos betalactâmicos. |
title |
Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams |
spellingShingle |
Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams Coelho, Isabelle Carvalho |
title_short |
Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams |
title_full |
Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams |
title_fullStr |
Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams |
title_full_unstemmed |
Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams |
title_sort |
Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams |
author |
Coelho, Isabelle Carvalho |
author_facet |
Coelho, Isabelle Carvalho Silva, Francisco Laurindo da Nunes, Maria do Rosário Conceição Moura Lopes, Liliana Silva Carneiro, Leandro Pessoa Ferreira, Pedro Henrique Piauilino Benvindo |
author_role |
author |
author2 |
Silva, Francisco Laurindo da Nunes, Maria do Rosário Conceição Moura Lopes, Liliana Silva Carneiro, Leandro Pessoa Ferreira, Pedro Henrique Piauilino Benvindo |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Coelho, Isabelle Carvalho Silva, Francisco Laurindo da Nunes, Maria do Rosário Conceição Moura Lopes, Liliana Silva Carneiro, Leandro Pessoa Ferreira, Pedro Henrique Piauilino Benvindo |
description |
Background and Objective: Klebsiella pneumoniae is a Gram negative bacilli causing infections related to health care. In recent years, it has become a multiressistente microorganism, because of the production spectrum beta-lactamase enzymes extended and carbapenemases. Therefore, the aim of this study was to evaluate the susceptibility profile of K. pneumoniae in relation to carbapenems; identifying carbapenemase production and to determine the susceptibility profile of the isolates compared to other β -lactams. Methods: This is research on naexperimental basis with quantitative and qualitative approach, which used 27 isolates from different clinical specimens ofpatients whit infection, assigned by reference laboratories. Prior to susceptibility testing, the saples were re-isolated on MacConckey Agar culture meddium and the plates were incubated with semeios at 37° C for 24 hours. Susceptibility testing to carbapenems and other beta-lactams were performed according to the method of Kirby-Bauer. The antibiotic disks used in the tests were amoxicillin - clavulanate, cefoxitin, cefotaxime, ceftazidime, cefepime, imipenem, meropenem and ertapenem. The test for the detection of the enzyme carbapenemase was performed by the use of antibiotics: meropenem, and ertapenem, by Modified Hodge test. Results:All isolates were susceptible to beta-lactam group of carbapenems and no production of carbapenemases, but 18.5 % produced extend-spectrum beta- lactamase extended spectrum and 40.7 % of the strains were resistant to other beta-lactams. Conclusion: The production of beta- lactamase extended spectrum was one of the main factors involved in resistance to antibiotics tested , but other resistance mechanisms were not identified possibly involved. KEY WORDS: Klebsiella pneumoniae. Disease Susceptibility. Beta-lactams. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-04-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://online.unisc.br/seer/index.php/epidemiologia/article/view/5468 10.17058/reci.v5i2.5468 |
url |
https://online.unisc.br/seer/index.php/epidemiologia/article/view/5468 |
identifier_str_mv |
10.17058/reci.v5i2.5468 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://online.unisc.br/seer/index.php/epidemiologia/article/view/5468/4294 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Unisc |
publisher.none.fl_str_mv |
Unisc |
dc.source.none.fl_str_mv |
Revista de Epidemiologia e Controle de Infecção; Vol. 5 No. 2 (2015); 79-83 Revista de Epidemiologia e Controle de Infecção; v. 5 n. 2 (2015); 79-83 2238-3360 reponame:Revista de Epidemiologia e Controle de Infecção instname:Universidade de Santa Cruz do Sul (UNISC) instacron:UNISC |
instname_str |
Universidade de Santa Cruz do Sul (UNISC) |
instacron_str |
UNISC |
institution |
UNISC |
reponame_str |
Revista de Epidemiologia e Controle de Infecção |
collection |
Revista de Epidemiologia e Controle de Infecção |
repository.name.fl_str_mv |
Revista de Epidemiologia e Controle de Infecção - Universidade de Santa Cruz do Sul (UNISC) |
repository.mail.fl_str_mv |
||liapossuelo@unisc.br|| julia.kern@hotmail.com||reci.unisc@gmail.com |
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