Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams

Detalhes bibliográficos
Autor(a) principal: Coelho, Isabelle Carvalho
Data de Publicação: 2015
Outros Autores: Silva, Francisco Laurindo da, Nunes, Maria do Rosário Conceição Moura, Lopes, Liliana Silva, Carneiro, Leandro Pessoa, Ferreira, Pedro Henrique Piauilino Benvindo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista de Epidemiologia e Controle de Infecção
Texto Completo: https://online.unisc.br/seer/index.php/epidemiologia/article/view/5468
Resumo: Background and Objective: Klebsiella pneumoniae is a Gram negative bacilli causing infections related to health care. In recent years, it has become a multiressistente microorganism, because of the production spectrum beta-lactamase enzymes extended and carbapenemases. Therefore, the aim of this study was to evaluate the susceptibility profile of K. pneumoniae in relation to carbapenems; identifying carbapenemase production and to determine the susceptibility profile of the isolates compared to other β -lactams. Methods: This is research on naexperimental basis with quantitative and qualitative approach, which used 27 isolates from different clinical specimens ofpatients whit infection, assigned by reference laboratories. Prior to susceptibility testing, the saples were re-isolated on MacConckey Agar culture meddium and the plates were incubated with semeios at 37° C for 24 hours. Susceptibility testing to carbapenems and other beta-lactams were performed according to the method of Kirby-Bauer. The antibiotic disks used in the tests were amoxicillin - clavulanate, cefoxitin, cefotaxime, ceftazidime, cefepime, imipenem, meropenem and ertapenem. The test for the detection of the enzyme carbapenemase was performed by the use of antibiotics: meropenem, and ertapenem, by Modified Hodge test. Results:All isolates were susceptible to beta-lactam group of carbapenems and no production of carbapenemases, but 18.5 % produced extend-spectrum beta- lactamase extended spectrum and 40.7 % of the strains were resistant to other beta-lactams. Conclusion: The production of beta- lactamase extended spectrum was one of the main factors involved in resistance to antibiotics tested , but other resistance mechanisms were not identified possibly involved. KEY WORDS: Klebsiella pneumoniae. Disease Susceptibility. Beta-lactams.
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spelling Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactamsAvaliação da suscetibilidade da Klebsiella pneumoniae aos betalactâmicos.Background and Objective: Klebsiella pneumoniae is a Gram negative bacilli causing infections related to health care. In recent years, it has become a multiressistente microorganism, because of the production spectrum beta-lactamase enzymes extended and carbapenemases. Therefore, the aim of this study was to evaluate the susceptibility profile of K. pneumoniae in relation to carbapenems; identifying carbapenemase production and to determine the susceptibility profile of the isolates compared to other β -lactams. Methods: This is research on naexperimental basis with quantitative and qualitative approach, which used 27 isolates from different clinical specimens ofpatients whit infection, assigned by reference laboratories. Prior to susceptibility testing, the saples were re-isolated on MacConckey Agar culture meddium and the plates were incubated with semeios at 37° C for 24 hours. Susceptibility testing to carbapenems and other beta-lactams were performed according to the method of Kirby-Bauer. The antibiotic disks used in the tests were amoxicillin - clavulanate, cefoxitin, cefotaxime, ceftazidime, cefepime, imipenem, meropenem and ertapenem. The test for the detection of the enzyme carbapenemase was performed by the use of antibiotics: meropenem, and ertapenem, by Modified Hodge test. Results:All isolates were susceptible to beta-lactam group of carbapenems and no production of carbapenemases, but 18.5 % produced extend-spectrum beta- lactamase extended spectrum and 40.7 % of the strains were resistant to other beta-lactams. Conclusion: The production of beta- lactamase extended spectrum was one of the main factors involved in resistance to antibiotics tested , but other resistance mechanisms were not identified possibly involved. KEY WORDS: Klebsiella pneumoniae. Disease Susceptibility. Beta-lactams.Justificativa e objetivos: A Klebsiellapneumoniae é um bacilo Gram negativo causador de infecções relacionadas à assistência à saúde. Nos últimos anos, tem se tornando um microrganismo multirresistente, em virtude da produção de enzimas beta-lactamases de espectro estendido e carbapenemases. O estudo teve como objetivos avaliar o perfil de suscetibilidade da K. pneumoniae em relação acarbapenêmicos; identificar produção de carbapenemases e determinar o perfil de suscetibilidade das cepas bacterianas em relação a outros β-lactâmicos. Métodos: Pesquisa com caráter experimental com abordagem quantiqualitativa. Utilizou-se 27 isolados, oriundos de diferentes espécimes clínicos de pacientes com vários tipos de infecção. Os testes de suscetibilidade aos beta-lactâmicos foram realizados pelo método de disco-difusão, seguindo o parâmetro de Kirby-Bauer. Nos testes de suscetibilidade foramutilizados discos de antibióticos, amoxicilina-clavulanato, cefoxitina, cefotaxima, ceftazidima, cefepime, imipenem, meropenem e ertapenem. Para a detecção de carbapenemase, mediante a utilização do teste de Hodge modificado foram utilizados os antibióticos meropenem, e ertapenem. Resultados: Entre ascepas de K. pneumoniae, utilizadas na pesquisa, 59,3% apresentaram suscetibilidade e 40,7% foram resistentes aos beta-lactâmicos, pertencentes ao grupo carbapenêmicos. Não foramidentificadas cepas produtoras de carbapenemases. 18,5% das cepas de K. pneumoniaeforam identificadas como produtoras de β-lactamase de espectro estendido. Conclusão: Com base nos resultados obtidos, a resistência que 18,5% das cepas de K. pneumoniae apresentaram em relação aos β-lactâmicosfoi em função da produção de β-lactamases de espectro estendido, haja visto, que o teste de identificação para essas enzimas foi realizado. Sugere-se mais pesquisas na identificação de cepas produtoras de carbapenemases, dado a grande ocorrência desses microrganimos em isolados clínicos de pacientes com infecção. DESCRITORES: Klebsiella pneumoniae. Susceptibilidade. Beta-lactâmicos.Unisc2015-04-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://online.unisc.br/seer/index.php/epidemiologia/article/view/546810.17058/reci.v5i2.5468Revista de Epidemiologia e Controle de Infecção; Vol. 5 No. 2 (2015); 79-83Revista de Epidemiologia e Controle de Infecção; v. 5 n. 2 (2015); 79-832238-3360reponame:Revista de Epidemiologia e Controle de Infecçãoinstname:Universidade de Santa Cruz do Sul (UNISC)instacron:UNISCporhttps://online.unisc.br/seer/index.php/epidemiologia/article/view/5468/4294Coelho, Isabelle CarvalhoSilva, Francisco Laurindo daNunes, Maria do Rosário Conceição MouraLopes, Liliana SilvaCarneiro, Leandro PessoaFerreira, Pedro Henrique Piauilino Benvindoinfo:eu-repo/semantics/openAccess2019-01-21T12:55:14Zoai:ojs.online.unisc.br:article/5468Revistahttps://online.unisc.br/seer/index.php/epidemiologia/indexONGhttp://online.unisc.br/seer/index.php/epidemiologia/oai||liapossuelo@unisc.br|| julia.kern@hotmail.com||reci.unisc@gmail.com2238-33602238-3360opendoar:2019-01-21T12:55:14Revista de Epidemiologia e Controle de Infecção - Universidade de Santa Cruz do Sul (UNISC)false
dc.title.none.fl_str_mv Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams
Avaliação da suscetibilidade da Klebsiella pneumoniae aos betalactâmicos.
title Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams
spellingShingle Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams
Coelho, Isabelle Carvalho
title_short Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams
title_full Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams
title_fullStr Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams
title_full_unstemmed Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams
title_sort Assessment of the susceptibility of Klebsiella pneumoniae to beta-lactams
author Coelho, Isabelle Carvalho
author_facet Coelho, Isabelle Carvalho
Silva, Francisco Laurindo da
Nunes, Maria do Rosário Conceição Moura
Lopes, Liliana Silva
Carneiro, Leandro Pessoa
Ferreira, Pedro Henrique Piauilino Benvindo
author_role author
author2 Silva, Francisco Laurindo da
Nunes, Maria do Rosário Conceição Moura
Lopes, Liliana Silva
Carneiro, Leandro Pessoa
Ferreira, Pedro Henrique Piauilino Benvindo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Coelho, Isabelle Carvalho
Silva, Francisco Laurindo da
Nunes, Maria do Rosário Conceição Moura
Lopes, Liliana Silva
Carneiro, Leandro Pessoa
Ferreira, Pedro Henrique Piauilino Benvindo
description Background and Objective: Klebsiella pneumoniae is a Gram negative bacilli causing infections related to health care. In recent years, it has become a multiressistente microorganism, because of the production spectrum beta-lactamase enzymes extended and carbapenemases. Therefore, the aim of this study was to evaluate the susceptibility profile of K. pneumoniae in relation to carbapenems; identifying carbapenemase production and to determine the susceptibility profile of the isolates compared to other β -lactams. Methods: This is research on naexperimental basis with quantitative and qualitative approach, which used 27 isolates from different clinical specimens ofpatients whit infection, assigned by reference laboratories. Prior to susceptibility testing, the saples were re-isolated on MacConckey Agar culture meddium and the plates were incubated with semeios at 37° C for 24 hours. Susceptibility testing to carbapenems and other beta-lactams were performed according to the method of Kirby-Bauer. The antibiotic disks used in the tests were amoxicillin - clavulanate, cefoxitin, cefotaxime, ceftazidime, cefepime, imipenem, meropenem and ertapenem. The test for the detection of the enzyme carbapenemase was performed by the use of antibiotics: meropenem, and ertapenem, by Modified Hodge test. Results:All isolates were susceptible to beta-lactam group of carbapenems and no production of carbapenemases, but 18.5 % produced extend-spectrum beta- lactamase extended spectrum and 40.7 % of the strains were resistant to other beta-lactams. Conclusion: The production of beta- lactamase extended spectrum was one of the main factors involved in resistance to antibiotics tested , but other resistance mechanisms were not identified possibly involved. KEY WORDS: Klebsiella pneumoniae. Disease Susceptibility. Beta-lactams.
publishDate 2015
dc.date.none.fl_str_mv 2015-04-04
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dc.identifier.uri.fl_str_mv https://online.unisc.br/seer/index.php/epidemiologia/article/view/5468
10.17058/reci.v5i2.5468
url https://online.unisc.br/seer/index.php/epidemiologia/article/view/5468
identifier_str_mv 10.17058/reci.v5i2.5468
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dc.relation.none.fl_str_mv https://online.unisc.br/seer/index.php/epidemiologia/article/view/5468/4294
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv Revista de Epidemiologia e Controle de Infecção; Vol. 5 No. 2 (2015); 79-83
Revista de Epidemiologia e Controle de Infecção; v. 5 n. 2 (2015); 79-83
2238-3360
reponame:Revista de Epidemiologia e Controle de Infecção
instname:Universidade de Santa Cruz do Sul (UNISC)
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instname_str Universidade de Santa Cruz do Sul (UNISC)
instacron_str UNISC
institution UNISC
reponame_str Revista de Epidemiologia e Controle de Infecção
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repository.name.fl_str_mv Revista de Epidemiologia e Controle de Infecção - Universidade de Santa Cruz do Sul (UNISC)
repository.mail.fl_str_mv ||liapossuelo@unisc.br|| julia.kern@hotmail.com||reci.unisc@gmail.com
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